The Material World of 3D‐Bioprinted and Microfluidic‐Chip Models of Human Liver Fibrosis DOI
Ana Margarida Carvalho, Ruchi Bansal, Cristina C. Barrias

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(2)

Published: Nov. 14, 2023

Biomaterials are extensively used to mimic cell-matrix interactions, which essential for cell growth, function, and differentiation. This is particularly relevant when developing in vitro disease models of organs rich extracellular matrix, like the liver. Liver involves a chronic wound-healing response with formation scar tissue known as fibrosis. At early stages, liver can be reverted, but progresses, reversion no longer possible, there cure. Research new therapies hampered by lack adequate that replicate mechanical properties biochemical stimuli present fibrotic Fibrosis associated changes composition matrix directly influence behavior. could play an role better emulating microenvironment. In this paper, recent cutting-edge biomaterials creating human fibrosis revised, combination cells, bioprinting, and/or microfluidics. These technologies have been instrumental intricate structure unhealthy promote medium perfusion improves growth respectively. A comprehensive analysis impact material hints cell-material interactions tridimensional context provided.

Language: Английский

A review on four-dimensional (4D) bioprinting in pursuit of advanced tissue engineering applications DOI
Zia Ullah Arif, Muhammad Yasir Khalid, Waqas Ahmed

et al.

Bioprinting, Journal Year: 2022, Volume and Issue: 27, P. e00203 - e00203

Published: March 30, 2022

Language: Английский

Citations

90
Integrated gradient tissue-engineered osteochondral scaffolds: Challenges, current efforts and future perspectives DOI Creative Commons

Xiaolian Niu,

Ning Li, Zhipo Du

et al.

Bioactive Materials, Journal Year: 2022, Volume and Issue: 20, P. 574 - 597

Published: July 1, 2022

The osteochondral defect repair has been most extensively studied due to the rising demand for new therapies diseases such as osteoarthritis. Tissue engineering proposed a promising strategy meet of simultaneous regeneration both cartilage and subchondral bone by constructing integrated gradient tissue-engineered scaffold (IGTEOS). This review brought forward main challenges establishing satisfactory IGTEOS from perspectives complexity physiology microenvironment tissue, limitations obtaining desired required scaffold. Then, we comprehensively discussed summarized current efforts resolve above challenges, including architecture strategies, fabrication techniques

Language: Английский

Citations

70
Classification, processing, and applications of bioink and 3D bioprinting: A detailed review DOI

Sania Raees,

Faheem Ullah, Fatima Javed

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 232, P. 123476 - 123476

Published: Jan. 31, 2023

Language: Английский

Citations

67
Recent Advances in Biodegradable and Biocompatible Synthetic Polymers Used in Skin Wound Healing DOI Open Access
Ruojiao Xu, Y.Z. Fang, Zhao Zhang

et al.

Materials, Journal Year: 2023, Volume and Issue: 16(15), P. 5459 - 5459

Published: Aug. 3, 2023

The treatment of skin wounds caused by trauma and pathophysiological disorders has been a growing healthcare challenge, posing great economic burden worldwide. use appropriate wound dressings can help to facilitate the repair healing rate defective skin. Natural polymer biomaterials such as collagen hyaluronic acid with excellent biocompatibility have shown promote restoration However, low mechanical properties fast degradation limited their applications. Skin based on biodegradable biocompatible synthetic polymers not only overcome shortcomings natural but also possess favorable for applications in wounds. Herein, we listed several used dressing materials, PVA, PCL, PLA, PLGA, PU, PEO/PEG, focusing composition, fabrication techniques, functions promoting healing. Additionally, future development prospects polymer-based are put forward. Our review aims provide new insights further using polymers.

Language: Английский

Citations

64
Recent progress of 3D printed vascularized tissues and organs DOI Creative Commons
Ke Zheng, Muyuan Chai,

Bingping Luo

et al.

Smart Materials in Medicine, Journal Year: 2024, Volume and Issue: 5(2), P. 183 - 195

Published: Jan. 12, 2024

Since the need for vascular networks to supply oxygen and nutrients while expelling metabolic waste, most cells can only survive within 200 μm of blood vessels; thus, construction well-developed vessel is essential manufacture artificial tissues organs. Three-dimensional (denoted as 3D) printing a scalable, reproducible high-precision manufacturing technology. In past several years, there have been many breakthroughs in building various vascularized tissues, greatly promoting development biological tissue engineering. This paper highlights latest progress 3D printed organs, including heart, liver, lung, kidney, penis. We also discuss application status potential above prospect further requirement technology clinically useable tissues.

Language: Английский

Citations

17
Applications of 3D Bioprinting in Tissue Engineering and Regenerative Medicine DOI Open Access
Gia Saini, Nicole Segaran, Joseph L. Mayer

et al.

Journal of Clinical Medicine, Journal Year: 2021, Volume and Issue: 10(21), P. 4966 - 4966

Published: Oct. 26, 2021

Regenerative medicine is an emerging field that centers on the restoration and regeneration of functional components damaged tissue. Tissue engineering application regenerative seeks to create tissue whole organs. Using 3D printing technologies, native mimics can be created utilizing biomaterials living cells. Recently, has begun employ bioprinting methods highly specialized models improve upon conventional methods. Here, we review use in advancement by describing process its advantages over other Materials techniques are also reviewed, addition future clinical applications, challenges, directions field.

Language: Английский

Citations

76
3D Cell Cultures: Evolution of an Ancient Tool for New Applications DOI Creative Commons
Andrea Cacciamali, Riccardo Villa, Silvia Dotti

et al.

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: July 22, 2022

Recently, research is undergoing a drastic change in the application of animal model as unique investigation strategy, considering an alternative approach for development science future. Although conventional monolayer cell cultures represent established and widely used vitro method, lack tissue architecture complexity such fails to inform true biological processes vivo . Recent advances culture techniques have revolutionized tools biomedical by creating powerful three-dimensional (3D) models recapitulate heterogeneity, structure functions primary tissues. These also bridge gap between traditional two-dimensional (2D) single-layer models. 3D systems allow researchers recreate human organs diseases one dish thus holds great promise many applications regenerative medicine, drug discovery, precision cancer research, gene expression studies. Bioengineering has made important contribution context using scaffolds that help mimic microenvironments which cells naturally reside, supporting mechanical, physical biochemical requirements cellular growth function. We therefore speak based on organoids, bioreactors, organ-on-a-chip up bioprinting each these provides its own advantages applications. All prove be excellent candidates methods testing, well revolutionizing technology. will able provide new ideas study interactions both basic more specialized compliance with 3R principle. In this review, we comparison 2D culture, details some different currently available discussing their strengths potential

Language: Английский

Citations

61
Recent Applications of Electrospun Nanofibrous Scaffold in Tissue Engineering DOI Creative Commons
Hamza Abu Owida, Jamal Al-Nabulsi, Feras Alnaimat

et al.

Applied Bionics and Biomechanics, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 15

Published: Feb. 9, 2022

Tissue engineering is a relatively new area of research that combines medical, biological, and fundamentals to create tissue-engineered constructs regenerate, preserve, or slightly increase the functions tissues. To mature tissue, extracellular matrix should be imitated by engineered structures, allow for oxygen nutrient transmission, release toxins during tissue repair. Numerous recent studies have been devoted developing three-dimensional nanostructures engineering. One most effective these methods electrospinning. nanofibrous scaffolds constructed over last few decades repair restoration. The current review gives an overview attempts construct meshes as various tissues such bone, cartilage, cardiovascular, skin Also, article addresses improvements difficulties in regeneration using

Language: Английский

Citations

44
3D Bioprinting of Novel κ-Carrageenan Bioinks: An Algae-Derived Polysaccharide DOI Creative Commons
Diana M. C. Marques, João C. Silva, Ana Paula Serro

et al.

Bioengineering, Journal Year: 2022, Volume and Issue: 9(3), P. 109 - 109

Published: March 6, 2022

Novel green materials not sourced from animals and with low environmental impact are becoming increasingly appealing for biomedical cellular agriculture applications. Marine biomaterials a rich source of structurally diverse compounds various biological activities. Kappa-carrageenan (κ-c) is potential candidate tissue engineering applications due to its gelation properties, mechanical strength, similar structural composition glycosaminoglycans (GAGs), possessing several advantages when compared other algae-based typically used in bioprinting such as alginate. For those reasons, this material was selected the main polysaccharide component bioinks developed herein. In work, pristine κ-carrageenan were successfully formulated first time fabricate 3D scaffolds by bioprinting. Ink formulation printing parameters optimized, allowing manufacturing complex structures. Mechanical compression tests dry weight determination revealed young's modulus between 24.26 99.90 kPa water contents above 97%. Biocompatibility assays, using mouse fibroblast cell line, showed high viability attachment. The bioprinted cells spread throughout exhibiting typical fibroblast-like morphology controls. bio-/printed structures remained stable under culture conditions up 11 days, preserving values. Overall, we established strategy manufacture through novel agriculture.

Language: Английский

Citations

39
Formulation and Evaluation of PVA/Gelatin/Carrageenan Inks for 3D Printing and Development of Tissue‐Engineered Heart Valves DOI Creative Commons
Arman Jafari, Seyyed Vahid Niknezhad, Maryam Kaviani

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 34(7)

Published: Oct. 10, 2023

Abstract Congenital and acquired valvular heart diseases (VHDs) are significant causes of mortality worldwide. With valve replacement being the primary solution for VHD, current options display shortcomings, including calcification, thrombogenicity, hemodynamic alteration, leading to repetitive surgeries. Tissue engineering, however, has shown great potential fabricating valves (HVs) with fewer complications. Here, a series inks developed, combining poly(vinyl alcohol), gelatin, carrageenan 3D printing tissue‐engineered (TEHVs). The inks/hydrogels investigated characterize their physico‐chemical, morphological, mechanical, rheological characteristics. In vitro in vivo biocompatibility, immune response, hemolysis, thrombogenicity also evaluated. Moreover, hydrodynamics TEHVs under physiological conditions reported. Inks demonstrate mechanical characteristics comparable native leaflets. Subcutaneous implantation reveals that hydrogels do not induce chronic inflammation can undergo remodeling. hemocompatibility assessments show minimal hemolysis low thrombogenicity. Different sizes types HVs successfully printed high fidelity air. hydrodynamic assessment confirms withstand aortic conditions. Altogether, 3D‐printed be promising alternative solve problems associated options.

Language: Английский

Citations

35