Breaking the limits in glycan recognition by NMR DOI Creative Commons
Jesús Jiménez‐Barbero

The Project Repository Journal, Journal Year: 2023, Volume and Issue: 17(1), P. 90 - 93

Published: July 3, 2023

Breaking the limits in glycan recognition by NMR (RECGLYCANMR) is a multidisciplinary project that combines state-of-the-art chemistry and chemical biology methods, including vitro in-cell NMR, biophysics protocols under crowding conditions to ask answer pivotal questions related sugar molecular Nature are intimately infection inflammation diseases.

Language: Английский

NMR investigations of glycan conformation, dynamics, and interactions DOI
Jesús Angulo, Ana Ardá, Sara Bertuzzi

et al.

Progress in Nuclear Magnetic Resonance Spectroscopy, Journal Year: 2024, Volume and Issue: 144-145, P. 97 - 152

Published: Oct. 12, 2024

Language: Английский

Citations

3
Con A lectin binding by synthetic bivalent arabinomannan tri- and pentasaccharides reveals connectivity-dependent functional valencies DOI

Ipsita Parija,

Shivender Yadav, Narayanaswamy Jayaraman

et al.

Carbohydrate Research, Journal Year: 2024, Volume and Issue: 536, P. 109050 - 109050

Published: Feb. 1, 2024

Language: Английский

Citations

2
Multivalent calix[4]arene-based mannosylated dendrons as new FimH ligands and inhibitors DOI
Alessandro Palmioli, Luca Moretti, Carlo Alberto Vezzoni

et al.

Bioorganic Chemistry, Journal Year: 2023, Volume and Issue: 138, P. 106613 - 106613

Published: May 18, 2023

Language: Английский

Citations

5
Fluorinated Man9 as a High Mannose Mimetic to Unravel Its Recognition by DC-SIGN Using NMR DOI Creative Commons

Adrián Silva-Díaz,

Jonathan Ramírez-Cárdenas, Juan C. Muñoz‐García

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(48), P. 26009 - 26015

Published: Nov. 18, 2023

Lectins are capable of reading out the structural information contained in carbohydrates through specific recognition processes. Determining binding epitope sugar is fundamental to understanding this event. Nuclear magnetic resonance (NMR) a powerful tool obtain solution; however, when involved complex oligosaccharide, such as high mannose, signal overlap found NMR spectra precludes an accurate analysis interaction. The introduction tags into these oligosaccharides could overcome problems and facilitate studies. Here, we show preparation Man9 mannose with some fluorine study interaction its receptor, dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN). This fluorinated ligand has allowed us apply heteronuclear two-dimensional (2D) 1H,19F STD-TOCSYreF experiments, using initial slope approach, which facilitated Man9/DC-SIGN interaction, unequivocally providing epitope.

Language: Английский

Citations

4
Bacterial Glycolipid Acting on Protein Transport Across Membranes DOI Creative Commons
Shoko Mori, Masafumi Shionyu, Keiko Shimamoto

et al.

ChemBioChem, Journal Year: 2024, Volume and Issue: 25(10)

Published: Feb. 24, 2024

Abstract The process of protein transport across membranes involves a variety factors and has been extensively investigated. Traditionally, proteinaceous translocons chaperones have recognized as crucial in this process. However, recent studies highlighted the significant roles played by lipids glycolipid present biological membrane transport. Membrane can influence efficiency altering physicochemical properties membranes. Notably, our revealed that diacylglycerol (DAG) attenuates mobility core region, leading to dramatic suppression integration. Conversely, Escherichia coli inner membranes, named integrase (MPIase), enhances integration not only through alteration but also via direct interactions with proteins. This review explores mechanisms mediated lipids, specifically DAG, MPIase. Our results, along employed analysis methods such fluorescence measurements, nuclear magnetic resonance, surface plasmon docking simulation, are presented elucidate these mechanisms.

Language: Английский

Citations

1
Biophysical Assays for Investigating Modulators of Macromolecular Complexes: An Overview DOI Creative Commons

Martina Garbagnoli,

Pasquale Linciano, Roberta Listro

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(16), P. 17691 - 17705

Published: April 10, 2024

Drug discovery is a lengthy and intricate process, in its early stage, crucial steps are the selection of therapeutic target identification novel ligands. Most targets dysregulated pathogenic cells; typically, their activation or deactivation leads to desired effect, while other cases, interfering with target-natural binder complex achieves results. Biophysical assays suitable strategy for finding new ligands interferent agents, being able evaluate ligand-protein interactions assessing effect small molecules (SMols) on macromolecular complexes. This mini-review provides detailed analysis widely used biophysical methods, including fluorescence-based approaches, circular dichroism, isothermal titration calorimetry, microscale thermophoresis, NMR spectroscopy. After brief description methodologies, examples interaction competition experiments described, together an advantages disadvantages each technique. overview most relevant technologies that can help identifying SMols not only bind proteins but also interfere

Language: Английский

Citations

1
Novel Pseudo-Two-Dimensional 19F NMR Spectroscopy for Rapid Simultaneous Detection of Amines in Complex Mixture DOI

Xijian Wu,

Xin Tong,

Biling Huang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(42), P. 16818 - 16824

Published: Oct. 10, 2024

Rapid detection of amines in complex mixtures presents a significant challenge. Here, we introduce novel nuclear magnetic resonance (NMR) method for amine utilizing probe with two fluorine atoms distinct chemical environments. Upon interaction an amine, the generates atomic peaks, which are used to create coordinates, revealing shifts on 19F NMR spectroscopy. This innovative approach allows clear distinction signals two-dimensional plane. has been effectively employed analyzing pharmaceuticals and amino acids Ophiopogon japonicus dry white wine, providing robust general analysis.

Language: Английский

Citations

1
A consensus structural motif for the capsular polysaccharide of Cryptococcus Neoformans by NMR/MD DOI Creative Commons
James H. Prestegard

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(7)

Published: Feb. 9, 2024

Language: Английский

Citations

0
In situ PET-RAFT polymerization to prepare guanidine-and-carbohydrate modified ZnO nanoparticles DOI

Junyan Zhao,

Yu Rao,

Hengyuan Zhang

et al.

Polymer Chemistry, Journal Year: 2024, Volume and Issue: 15(21), P. 2140 - 2147

Published: Jan. 1, 2024

ZnO–polymer core–shell nanoparticles were successfully prepared using a simple in situ open-to-air PET-RAFT method.

Language: Английский

Citations

0
JoVE Video Dataset DOI Open Access
Sara Bertuzzi, Ana Poveda, Ana Ardá

et al.

Published: May 19, 2024

The interactions of glycans with proteins modulate many events related to health and disease. In fact, the establishment these recognition their biological consequences are intimately three-dimensional structures both partners, as well dynamic features presentation on corresponding cell compartments. NMR techniques unique disentangle characteristics and, indeed, diverse NMR-based methodologies have been developed applied monitor binding associate receptors. This protocol outlines procedures acquire, process analyze two most powerful employed in NMR-glycobiology field, 1H-Saturation transfer difference (STD) 1H,15N-Heteronuclear single quantum coherence (HSQC) titration experiments, which complementarily offer information from glycan protein perspective, respectively. Indeed, when combined they a toolkit for elucidating structural aspects molecular processes. comprehensive approach enhances our understanding glycan-protein contributes advancing research chemical glycobiology field.

Language: Английский

Citations

0