Operando Decoding Ion‐Conductive Switch in Stimuli‐Responsive Hydrogel by Nanodiamond‐Based Quantum Sensing DOI Creative Commons
Ruqiang Dou, Zan Li, Guoli Zhu

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Thermal-responsive hydrogels are developed as ion-conductive switchs for energy storage devices, however, the molecule mechanism of switch on/off remains unclear. Here, poly(N-isopropylacrylamide-co-acrylamide) hydrogel is synthesized a model material and nanodiamond (ND) based quantum sensing phase change study developed. First, micro-scale separation with cross-linked mesh structure after sol-gel transition visualized in situ water molecules trapped by polymer chains on chemically "frozen" state. Then, nano-scale inhomogeneous distributions viscosity, thermal conductivity ionic mobility at high temperature observed measuring rotation, translation zero-field splitting NDs. Besides, found to be dependent not only but also concentration. These observations suggested that physical "wall" induced microscopic scale blocked ion conduction pathways, providing potential intrinsic explanation migration shut-down temperature.

Language: Английский

Lift-CM: An integrated Lift-heater Centrifugal Microfluidic Platform for point-of-care pathogen nucleic acid detection using Isothermal amplification and CRISPR/Cas12a DOI

Taowei Shu,

Xueer Yin,

Qihua Xiong

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 274, P. 117178 - 117178

Published: Jan. 18, 2025

Language: Английский

Citations

2
Tuning the Dynamic Reaction Balance of CRISPR/Cas12a and RPA in One Pot: A Key to Switch Nucleic Acid Quantification DOI
Zhihao Yao, Kaiyu He, Hongmei Wang

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(7), P. 3511 - 3519

Published: April 23, 2024

Excavating nucleic acid quantitative capabilities by combining clustered regularly interspaced short palindromic repeats (CRISPR) and isothermal amplification in one pot is of common interest. However, the mutual interference between CRISPR cleavage primary obstacle to detection. Though several works have demonstrated enhanced detection sensitivity reducing inhibition on pot, few paid attention process even dynamic reaction processes two. Herein, we find that DNA quantification can be realized regulating either recombinase polymerase (RPA) efficiency or CRISPR/Cas12a cleaving (namely, tuning balance) pot. The sensitive utilizing dual PAM-free crRNAs for recognition. varied RPA primer concentration with stabilized systems significantly affects performances. Alternatively, also achieved stabilizing while concentration. capability proved detecting targets from Lactobacillus acetotolerans SARS-CoV-2. performance toward real samples comparable real-time PCR L. spiked fermented food SARS-CoV-2 clinical samples. We expect presented method will a powerful tool quantifying other targets.

Language: Английский

Citations

14
A Machine Vision Perspective on Droplet‐Based Microfluidics DOI Creative Commons
Ji‐Xiang Wang, Hongmei Wang,

Huang Lai

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Abstract Microfluidic droplets, with their unique properties and broad applications, are essential in chemical, biological, materials synthesis research. Despite the flourishing studies on artificial intelligence‐accelerated microfluidics, most research efforts have focused upstream design phase of microfluidic systems. Generating user‐desired droplets still remains laborious, inefficient, time‐consuming. To address long‐standing challenges associated accurate efficient identification, sorting, analysis morphology generation rate single double emulsion a novel machine vision approach utilizing deformable detection transformer (DETR) algorithm is proposed. This method enables rapid precise (detection relative error < 4% precision > 94%) across various scales scenarios, including real‐world simulated environments. identification (MDIA), web‐based tool powered by Deformable DETR, which supports transfer learning to enhance accuracy specific user scenarios developed. MDIA characterizes diameter, number, frequency, other parameters. As more training data added users, MDIA's capability universality expand, contributing comprehensive database for droplet microfluidics. The work highlights potential intelligence advancing regulation, fabrication, label‐free analysis, accelerating biochemical sciences engineering.

Language: Английский

Citations

1
Dual-directional small-sampling deep-learning modelling on co-flowing microfluidic droplet generation DOI
Ji‐Xiang Wang,

Jian Qian,

Hongmei Wang

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 485, P. 149467 - 149467

Published: Feb. 10, 2024

Language: Английский

Citations

7
CRISPR/Cas12a triggered SERS and naked eye dual-mode biosensor for ultrasensitive and on-site detection of nucleic acid via cascade signal amplification DOI
Jianghua Liu, Jiahui Chen, Shijie Jia

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 404, P. 135249 - 135249

Published: Jan. 2, 2024

Language: Английский

Citations

6
High-Throughput and Integrated CRISPR/Cas12a-Based Molecular Diagnosis Using a Deep Learning Enabled Microfluidic System DOI
Li Zhang, Huili Wang, Sheng Yang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(35), P. 24236 - 24251

Published: Aug. 22, 2024

CRISPR/Cas-based molecular diagnosis demonstrates potent potential for sensitive and rapid pathogen detection, notably in SARS-CoV-2 mutation tracking. Yet, a major hurdle hindering widespread practical use is its restricted throughput, limited integration, complex reagent preparation. Here, system, microfluidic multiplate-based ultrahigh throughput analysis of variants concern using CRISPR/Cas12a nonextraction RT-LAMP (mutaSCAN), proposed detection with resource requirements. With the aid self-developed reagents deep-learning enabled prototype device, our mutaSCAN system can detect mock swab samples below 30 min as low 250 copies/mL up to 96 per round. Clinical specimens were tested this accuracy routine testing (22 wildtype samples, 26 mutational samples) was 98% 100%, respectively. No false-positive results found negative (n = 24) samples.

Language: Английский

Citations

5
RNA-Dependent RNA Targeting by CRISPR-Cas Systems: Characterizations and Applications DOI Open Access

Natalia M. Gunitseva,

M.A. Evteeva, Anna S. Borisova

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 6894 - 6894

Published: April 7, 2023

Genome editing technologies that are currently available and described have a fundamental impact on the development of molecular biology medicine, industrial agricultural biotechnology other fields. However, genome based detection manipulation targeted RNA is promising alternative to control gene expression at spatiotemporal transcriptomic level without complete elimination. The innovative CRISPR-Cas RNA-targeting systems changed conception biosensing also allowed effectors be used in various applications; for example, genomic editing, effective virus diagnostic tools, biomarkers, transcription regulations. In this review, we discussed current state-of-the-art specific known bind cleave substrates summarized potential applications versatile systems.

Language: Английский

Citations

13
Recent advances in the CRISPR/Cas system-based visual detection method DOI
Jinrong Chen, Hang Su, June Hyun Kim

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Reviews the development of visual detection methods in recent years based on different Cas and analyzes their advantages disadvantages as well challenges future research.

Language: Английский

Citations

4
Harnessing the power of clustered regularly interspaced short palindromic repeats (CRISPR) based microfluidics for next-generation molecular diagnostics DOI
Rasanpreet Kaur, Saurabh Kumar Gupta, Arjun Chauhan

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: Aug. 8, 2024

Language: Английский

Citations

4
Leveraging Synthetic Antibody–DNA Conjugates to Expand the CRISPR-Cas12a Biosensing Toolbox DOI
Elisa Paialunga,

N. Bagheri,

Marianna Rossetti

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

We report here the use of antibody-DNA conjugates (Ab-DNA) to activate collateral cleavage activity CRISPR-Cas12a enzyme. Our findings demonstrate that Ab-DNA effectively trigger CRISPR-Cas12a, enabling transduction antibody-mediated recognition events into fluorescence outputs. developed two different immunoassays using an as activator Cas12a: CRISPR-based immunosensing assay (CIA) for detecting SARS-CoV-2 spike S protein, which shows superior sensitivity compared with traditional enzyme-linked immunosorbent (ELISA), and immunomagnetic (CIMA). Notably, CIMA successfully detected protein in undiluted saliva a limit detection (LOD) 890 pM 2 h assay. results underscore benefits integrating Cas12a-based signal amplification antibody methods. The potential conjugates, combined CRISPR technology, offers promising alternative conventional enzymes used could facilitate development versatile analytical platforms non-nucleic acid targets.

Language: Английский

Citations

0