Natural Products and Bioprospecting,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: March 13, 2025
Abstract
Phytochemical
study
of
the
n
-BuOH
extract
Ilex
asprella
resulted
in
discovery
ten
new
pentacyclic
triterpenoid
glycosides,
comprising
nine
ursane-type
glycosides
(
1
−
9
)
and
one
oleanane-type
glycoside
10
),
along
with
seven
known
compounds
11
17
).
Compound
is
first
reported
19,22-epoxy
ursane
glycoside,
whereas
4
5
are
rare
examples
containing
a
28,19-lactone
group.
The
structural
characterization
these
was
achieved
using
spectroscopic
chemical
techniques,
as
well
single-crystal
X-ray
analysis.
Compounds
7
,
12
15
exhibited
moderate
cytotoxic
activities
against
H1975
HCC827
cancer
cells.
Graphical
abstract
Computational and Structural Biotechnology Journal,
Journal Year:
2023,
Volume and Issue:
21, P. 5358 - 5371
Published: Jan. 1, 2023
Natural
products
synthesized
by
plants
have
substantial
industrial
and
medicinal
values
are
therefore
attracting
increasing
interest
in
various
related
industries.
Among
the
key
enzyme
families
involved
biosynthesis
of
natural
products,
uridine
diphosphate-dependent
glycosyltransferases
(UGTs)
play
a
crucial
role
plants.
In
recent
years,
significant
efforts
been
made
to
elucidate
catalytic
mechanisms
substrate
recognition
plant
UGTs
improve
them
for
desired
functions.
this
review,
we
presented
comprehensive
overview
all
currently
published
structures
UGTs,
along
with
in-depth
analyses
corresponding
mechanisms.
addition,
summarized
evaluated
protein
engineering
strategies
applied
activities
particular
focus
on
high-throughput
screening
methods.
The
primary
objective
review
is
provide
readers
understanding
serve
as
valuable
reference
latest
techniques
used
their
activities.
ACS Synthetic Biology,
Journal Year:
2024,
Volume and Issue:
13(4), P. 1059 - 1076
Published: March 28, 2024
Triterpenoids
possess
a
range
of
biological
activities
and
are
extensively
utilized
in
the
pharmaceutical,
food,
cosmetic,
chemical
industries.
Traditionally,
they
acquired
through
synthesis
plant
extraction.
However,
these
methods
have
drawbacks,
including
high
energy
consumption,
environmental
pollution,
being
time-consuming.
Recently,
de
novo
triterpenoids
microbial
cell
factories
has
been
achieved.
This
represents
promising
environmentally
friendly
alternative
to
traditional
supply
methods.
Saccharomyces
cerevisiae,
known
for
its
robustness,
safety,
ample
precursor
supply,
stands
out
as
an
ideal
candidate
triterpenoid
biosynthesis.
challenges
persist
industrial
production
economic
feasibility
Consequently,
metabolic
engineering
approaches
applied
improve
yield,
leading
substantial
progress.
review
explores
biosynthesis
mechanisms
S.
cerevisiae
strategies
efficient
production.
Finally,
also
discusses
current
proposes
potential
solutions,
offering
insights
future
engineering.
Applied Microbiology and Biotechnology,
Journal Year:
2024,
Volume and Issue:
108(1)
Published: Feb. 21, 2024
Abstract
Terpenoids
are
a
class
of
structurally
complex,
naturally
occurring
compounds
found
predominantly
in
plant,
animal,
and
microorganism
secondary
metabolites.
Classical
terpenoids
typically
have
carbon
atoms
multiples
five
follow
well-defined
skeletons,
whereas
noncanonical
deviate
from
these
patterns.
These
often
result
the
methyltransferase-catalyzed
methylation
modification
substrate
units,
leading
to
irregular
skeletons.
In
this
comprehensive
review,
various
activities
applications
terpenes
been
summarized.
Importantly,
review
delves
into
biosynthetic
pathways
terpenes,
including
those
with
C6,
C7,
C11,
C12,
C16
bacteria
fungi
host.
It
also
covers
triterpenes
synthesized
non-squalene
substrates
nortriterpenes
Ganoderma
lucidum
,
providing
detailed
examples
elucidate
intricate
processes
involved.
Finally,
outlines
potential
future
terpenoids.
conclusion,
insights
gathered
provide
reference
for
understanding
biosynthesis
pave
way
discovery
additional
unique
novel
terpenes.
Key
points
•
The
introduced
.
skeletons
presented
microbial
is
summarized
Molecules,
Journal Year:
2023,
Volume and Issue:
28(3), P. 1437 - 1437
Published: Feb. 2, 2023
Ginsenosides
are
major
bioactive
compounds
present
in
the
Panax
species.
exhibit
various
pharmaceutical
properties,
including
anticancer,
anti-inflammatory,
antimetastatic,
hypertension,
and
neurodegenerative
disorder
activities.
Although
several
commercial
products
have
been
presented
on
market,
most
of
current
chemical
processes
an
unfriendly
environment
a
high
cost
downstream
processing.
Compared
to
plant
extraction,
microbial
production
exhibits
efficiency,
selectivity,
saves
time
for
manufacturing
industrial
products.
To
reach
full
potential
resource
ginsenoside,
suitable
microorganism
has
developed
as
novel
approach.
In
this
review,
cell
biological
mechanisms
anticancer
activities
state
research
ginsenosides
summarized.
Microbial
hosts,
native
endophytes
engineered
microbes,
used
promising
approaches.
Furthermore,
challenges
perspectives
using
hosts
produce
discussed.
The Plant Journal,
Journal Year:
2024,
Volume and Issue:
118(3), P. 731 - 752
Published: Jan. 16, 2024
SUMMARY
Prunella
vulgaris
is
one
of
the
bestselling
and
widely
used
medicinal
herbs.
It
recorded
as
an
ace
medicine
for
cleansing
protecting
liver
in
Chinese
Pharmacopoeia
has
been
main
constitutions
many
herbal
tea
formulas
China
centuries.
also
a
traditional
folk
Europe
other
countries
Asia.
Pentacyclic
triterpenoids
are
major
class
bioactive
compounds
produced
P.
.
However,
their
biosynthetic
mechanism
remains
to
be
elucidated.
Here,
we
report
chromosome‐level
reference
genome
using
approach
combining
Illumina,
ONT,
Hi‐C
technologies.
671.95
Mb
size
with
scaffold
N50
49.10
complete
BUSCO
98.45%.
About
98.31%
sequence
was
anchored
into
14
pseudochromosomes.
Comparative
analysis
revealed
recent
WGD
Genome‐wide
identified
35
932
protein‐coding
genes
(PCGs),
which
59
encode
enzymes
involved
2,3‐oxidosqualene
biosynthesis.
In
addition,
10
PvOSC
,
358
PvCYP
177
PvUGT
were
identified,
five
PvOSCs
25
PvCYPs
9
PvUGTs
predicted
biosynthesis
pentacyclic
triterpenoids.
Biochemical
activity
assay
PvOSC2,
PvOSC4,
PvOSC6
recombinant
proteins
showed
that
they
mixed
amyrin
synthase
(MAS),
lupeol
(LUS),
β‐amyrin
(BAS),
respectively.
The
results
provide
solid
foundation
further
elucidating
