Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens DOI Creative Commons
Hanh An Nguyen, Nae Yoon Lee

Sensors, Journal Year: 2023, Volume and Issue: 23(22), P. 9112 - 9112

Published: Nov. 11, 2023

A pipette-free and fully integrated device that can be used to accurately recognize the presence of infectious pathogens is an important useful tool in point-of-care testing, particularly when aiming decrease unpredictable threats posed by disease outbreak. In this study, a paper developed integrate three main processes required for detecting pathogens, including DNA extraction, loop-mediated isothermal amplification (LAMP), detection. All key reagents, sodium dodecyl sulfate (SDS), NaOH, LAMP carmoisine, are placed on device. The operated simply via sliding folding without using any bulky equipment, results directly observed naked eye. optimized concentrations hydroxide (NaOH), carmoisine were found 0.1%, 0.1 M, 0.5 mg/mL, respectively. was detect Enterococcus faecium at as low 102 CFU/mL within 60 min. Also, E. spiked milk successfully detected device, demonstrating feasible application real sample analysis.

Language: Английский

Nanoparticle‐Based Bioaffinity Assays: From the Research Laboratory to the Market DOI Creative Commons
Zdeněk Farka, Julian C. Brandmeier, Matthias J. Mickert

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(3)

Published: Dec. 2, 2023

Abstract Advances in the development of new biorecognition elements, nanoparticle‐based labels as well instrumentation have inspired design bioaffinity assays. This review critically discusses potential nanoparticles to replace current enzymatic or molecular immunoassays and other Successful implementations commercial assays need for rapid tests incorporating different roles such capture support, signal generation amplification systems are highlighted. The limited number applied can be explained by challenges associated with analysis real samples (e.g., blood, urine, nasal swabs) that difficult resolve, particularly if same performance achieved more easily conventional labels. Lateral flow based on visual detection red‐colored line formed colloidal gold a notable exception, exemplified SARS‐CoV‐2 antigen moved from initial laboratory testing widespread market adaption less than two years.

Language: Английский

Citations

26
Point-of-care testing: state-of-the art and perspectives DOI Creative Commons
Mario Plebani, James H. Nichols, Peter B. Luppa

et al.

Clinical Chemistry and Laboratory Medicine (CCLM), Journal Year: 2024, Volume and Issue: unknown

Published: June 17, 2024

Abstract Point-of-care testing (POCT) is becoming an increasingly popular way to perform laboratory tests closer the patient. This option has several recognized advantages, such as accessibility, portability, speed, convenience, ease of use, ever-growing test panels, lower cumulative healthcare costs when used within appropriate clinical pathways, better patient empowerment and engagement, reduction certain pre-analytical errors, especially those related specimen transportation. On other hand, POCT also poses some limitations risks, namely risk accuracy reliability compared traditional tests, quality control connectivity issues, high dependence on operators (with varying levels expertise or training), challenges data management, higher per individual test, regulatory compliance issues need for validation prior use (especially rapid diagnostic tests; RDTs), well additional preanalytical sources error that may remain undetected in this type testing, which usually based whole blood samples (i.e., presence interfering substances, clotting, hemolysis, etc.). There no doubt a breakthrough innovation medicine, but discussion its requires further debate initiatives. collective opinion paper, composed abstracts lectures presented at two-day expert meeting “Point-Of-Care-Testing: State Art Perspective” (Venice, April 4–5, 2024), aims provide thoughtful overview state-of-the-art POCT, current applications, advantages potential limitations, interesting reflections future perspectives particular field medicine.

Language: Английский

Citations

15
Ag‐Cu Nanoarchitecture for Enhanced LSPR Absorption: the Role of Surface Roughness and near‐field Interactions DOI Creative Commons
Sanjay Kumar, Pavel Čurda, Hana Mašková

et al.

Advanced Materials Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract The plasmonic properties and biosensing performance of a nanocomposite with incorporated Cu Ag nanoparticles (NPs) are studied experimentally. concept's viability is verified by detecting specific anti‐decorin‐binding protein A (DbpA) from Borrelia afzelii antibodies, typically produced living organism in response to Lyme disease infection, carried out real‐life negative positive blood sera. It shown that the addition NPs on top silver regularly boosts absorption caused optical electrical changes vicinity surface compared only. Finite‐difference time‐domain (FDTD) simulations near‐field interaction different environments propose suitable Cu–Ag coupling modes responsible for modulation localized Surface Plasmon Resonance (LSPR) bands. Furthermore, increased roughness, resulting architecture combined metals, significantly enlarges effective area sensing applications, which consequentially leads better species immobilization. observed distinguishable differences between sera LSPR response, high sensitivity selectivity together stability (suppressed aging) opens door concepts rapid, low‐cost, sensitive LPSR‐based biodetection platforms.

Language: Английский

Citations

1
A nanoparticle-assisted signal-enhancement technique for lateral flow immunoassays DOI
Fang Gao, Shaonian Ye, Lin Huang

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(28), P. 6735 - 6756

Published: Jan. 1, 2024

Lateral flow immunoassay (LFIA), an affordable and rapid paper-based detection technology, is employed extensively in clinical diagnosis, environmental monitoring, food safety analysis. The COVID-19 pandemic underscored the validity adoption of LFIA performing large-scale public health testing. unprecedented demand for prompt diagnostic responses advances nanotechnology have fueled rise next-generation technologies. utilization nanoparticles to amplify signals represents innovative approach aimed at augmenting sensitivity. This review probes nanoparticle-assisted amplification strategies applications secure low limits expedited response rates. Emphasis placed on comprehending correlation between physicochemical properties performance. Lastly, we shed light challenges opportunities this prolific field.

Language: Английский

Citations

5
Optofluidic biosensing: Devices, strategies, and applications DOI Creative Commons
Genni Testa, Gianluca Persichetti, Romeo Bernini

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 178, P. 117865 - 117865

Published: July 10, 2024

Optofluidics is an interdisciplinary domain merging optics and microfluidics has driven significant progress in the development of small-scale, high-performance optical biosensors, aiming at final concept lab-on-chip. The successful these outcomes requires overcoming challenges related to effective integration optics, microfluidics, biorecognition elements a micron-scale level. opportunities offered by optofluidics, including efficient liquid handling strong light-matter interaction, have inspired creation many optofluidic biosensors with high performance versatile applications, ranging from single molecule detection up cell analysis. review presents critically analyzes various schemes their fabrication techniques. Different strategies bioassays are examined for impact on device feasibility. Finally, manuscript discusses open future trends this field, pointing towards ongoing evolution as promising area research application.

Language: Английский

Citations

5
Immunochromatographic method based on PCN-222 with dual-signal output for the rapid and sensitive detection of furazolidone metabolites in animal-derived food DOI Creative Commons

Siyao Zhong,

Haizhi Huang, Xiaoyao Wang

et al.

LWT, Journal Year: 2024, Volume and Issue: 198, P. 115996 - 115996

Published: March 25, 2024

The residues of furazolidone and its metabolites (3-amino-2-oxazolidinone [AOZ]) in food would have adverse effects on humans. Herein, a colorimetric fluorescent dual-signal output immunochromatographic method with zirconium-based porphyrin metal-organic framework (PCN-222) as the signal label was developed for detection 3-[(4-carboxyphenyl) monomethyl] amino-2-oxazolidinone (CPAOZ), derivative metabolite. PCN-222 synthesized using solvothermal method, material characterized via scanning electron microscopy, Fourier-transform-infrared spectroscopy, powder X-ray diffraction, which proved successful synthesis PCN-222. Synthesized PCN-222, nanotag, bound to antibodies prepare PCN-222-mAbs dual optical properties. Under optimized conditions, PCN-222- lateral flow assay (LFA) exhibited great linear relationship range 0.25–100 ng/mL. visual limit CPAOZ signals were 1.0 ng/mL 0.5 ng/mL, respectively, sensitivity 5- 10-fold higher than that colloidal gold-based LFA method. In addition, PCN-222-LFA successfully applied shrimp, chicken milk samples achieving satisfactory recoveries from 92.34% 117.44%. This study provides an alternative, convenient sensitive rapid veterinary drug residues.

Language: Английский

Citations

4
Advanced techniques and nanotechnologies for point-of-care testing DOI Creative Commons
Valeria Secchi, Alice Armanni, Linda Barbieri

et al.

Frontiers in Nanotechnology, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 15, 2025

Hospitals and other healthcare facilities harbor a complex interplay of microbial pathogens. The correct understanding pathogens distribution evolution is therefore crucial for infections control the design effective prevention strategies. In parallel, integration cutting-edge nanotechnologies early detection monitoring these specific target considered most approach to face nosocomial infections. this context, point-of-care (POC) testing, also known as near-patient becoming increasingly important. review we provide systematic insight into recent scientific technological advances in pathogen that explore advanced realize devices nanobiosensors, with improved selectivity sensitivity. particular, report on diffused affordable developed still developing POC aim increase sensitivity, speed accuracy different environments, from intensive care units outside laboratory hospital settings. text sub-divided several sections, each one focused type nanomaterials techniques actually employed.

Language: Английский

Citations

0
Three-dimensional windmill-shaped CAuNP based on chirality transfer and size engineering for highly sensitive immunochromatography assay DOI

Zhengzheng Wang,

Yuting Shang,

Liqing Xi

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160079 - 160079

Published: Jan. 1, 2025

Language: Английский

Citations

0
A review: early detection of oral cancer biomarkers using microfluidic colorimetric point-of-care devices DOI Creative Commons
Aniket Balapure, Satish Kumar Dubey, Arshad Javed

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(36), P. 6098 - 6118

Published: Jan. 1, 2024

A comprehensive salivary biomarker study for oral cancer identification is carried out. Lateral flow assays (LFAs) and paper-based devices, low-resource settings, have been extensively examined.

Language: Английский

Citations

3
Research progress and application exploration of typical microreactor technologies for health monitoring and disease Diagnosis/Treatment DOI

Zhongjian Tan,

Yun Zheng, Huanhuan Shi

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 155938 - 155938

Published: Sept. 1, 2024

Language: Английский

Citations

3