Talanta, Journal Year: 2024, Volume and Issue: 285, P. 127402 - 127402
Published: Dec. 16, 2024
Language: Английский
ACS Nano, Journal Year: 2024, Volume and Issue: 18(36), P. 25155 - 25169
Published: Aug. 27, 2024
Steviol glycosides (SGs) are a class of high-potency noncalorie natural sweeteners made up common diterpenoid core and varying glycans. Thus, the diversity glycans in composition, linkage, isomerism results tremendous structural complexity SG family, which poses challenges for precise identification leads to fact that SGs frequently used mixtures their variances biological activity remain largely unexplored. Here we show wild-type aerolysin nanopore can detect discriminate diverse species through modulable electro-osmotic flow effect at varied applied voltages. At low voltages, neutral molecule was drawn stuck pore entrance due an energy barrier around R220 sites. The ensuing binding events enable majority species. Increasing voltage break cause translocation events, allowing unambiguous several pairs differing by only one hydroxyl group recognition accumulation from multiple sensing regions Based on data 15 SGs, deep learning-based artificial intelligence (AI) model created process individual blockage achieving rapid, automated, single-molecule quantification real samples. This work highlights value analysis complex glycans-containing glycosides, as well potential sensitive rapid quality assurance glycoside products with use AI.
Language: Английский
Citations
5
Organic & Biomolecular Chemistry, Journal Year: 2024, Volume and Issue: 22(38), P. 7767 - 7785
Published: Jan. 1, 2024
Glycan is an essential cell component that usually exists in either a free form or glycoconjugated form. Glycosylation affects the regulatory function of glycoconjugates health and disease development, indicating key role glycan organisms. Because complexity diversity structures, it challenging to prepare structurally well-defined glycans, which hinders investigation biological functions at molecular level. Chemoenzymatic synthesis attractive approach for preparing complex because avoids tedious protecting group manipulations chemical ensures high regio- stereo-selectivity glucosides during assembly. Herein, enzymes, such as glycosyltransferases (GTs) glycosidases (GHs), sugar donors involved chemoenzymatic human glycans are initially discussed. Many state-of-the-art methodologies subsequently displayed summarized illustrate development synthetic example,
Language: Английский
Citations
4
Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 2, 2025
Nanopores are promising sensors for glycan analysis with the accurate identification of complex glycans laying foundation nanopore-based sequencing. However, their applicability toward continuous sequencing has not yet been demonstrated. Here, we present a proof-of-concept by combining nanopore technology glycosidase-hydrolyzing reactions. By continuously monitoring changes in characteristic current generated translocation hydrolysis products through nanopore, sequence can be accurately identified based on specificity glycosidases. With machine learning, improved accuracy to over 98%, allowing reliable determination consecutive building blocks and glycosidic linkages chains while reducing need operator expertise. This approach was validated real samples, calibrated using hydrophilic interaction chromatography-high-performance liquid chromatography (HILIC-HPLC) mass spectrometry (MS). We achieved ten units natural chains, which provided first evidence feasibility nanopore-glycosidase-compatible system Compared traditional methods, this strategy enhances efficiency 5-fold. Additionally, introduced concept 'inverse sequencing', focuses electrical signal rather than monosaccharide identification. eliminates reliance fingerprint libraries typically required putative 'forward hydrolysis' strategies. When challenges both inverse strategies' addressed, will pave way establishing at single-molecule level.
Language: Английский
Citations
0
Talanta, Journal Year: 2025, Volume and Issue: 286, P. 127544 - 127544
Published: Jan. 10, 2025
Language: Английский
Citations
0
Soft Matter, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Nanopore sequencing technology has revolutionized single-molecule analysis through its unique capability to detect and characterize individual biomolecules with unprecedented precision.
Language: Английский
Citations
0
Sensors, Journal Year: 2024, Volume and Issue: 24(16), P. 5442 - 5442
Published: Aug. 22, 2024
Saccharides, being one of the fundamental molecules life, play essential roles in physiological and pathological functions cells. However, their intricate structures pose challenges for detection. Nanopore technology, with its high sensitivity capability single-molecule-level analysis, has revolutionized identification structural analysis saccharide molecules. This review focuses on recent advancements nanopore technology carbohydrate detection, presenting an array methods that leverage molecular complexity saccharides. Biological techniques utilize specific protein binding or pore modifications to trigger typical resistive pulses, enabling high-sensitivity detection monosaccharides oligosaccharides. In solid-state sensing, boronic acid modification pH gating mechanisms are employed recognition quantitative polysaccharides. The integration artificial intelligence algorithms can further enhance accuracy reliability analyses. Serving as a crucial tool we foresee significant potential application disease diagnosis, drug screening, biosensing, fostering innovative progress related research domains.
Language: Английский
Citations
2
ChemBioChem, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 5, 2024
Abstract This prospect explores the integration of enrichment strategies with nanopore detection to advance clinical glycoproteomics. Glycoproteins, crucial for understanding biological processes, pose challenges due their low abundance and structural diversity. Enrichment techniques using lectin affinity, boronate hydrazide chemistry especially molecular imprinted polymers may selectively specifically isolate glycoproteins from complex samples, while technology enables label‐free, real‐time, single‐molecule analysis. approach holds promise disease‐related glycosylation studies, biomarker discovery, personalized medicine, streamlined Standardization, optimization, data analysis remain challenges, requiring interdisciplinary collaborations technological advancements. Overall, this offer transformative potential glycoproteomics innovative diagnostic therapeutic strategies.
Language: Английский
Citations
0
The Journal of Physical Chemistry Letters, Journal Year: 2024, Volume and Issue: unknown, P. 11792 - 11800
Published: Nov. 18, 2024
Nanopore sensing is now reshaping analytical proteomics with its simplicity, convenience, and high sensitivity. Determining the length of polyglutamine (polyQ) crucial for rapid screening Huntington's disease. In this computational study, we present a cross-nanoslit detection approach to determine polyQ length, where nanoslit carved within two-dimensional (2D) in-plane heterostructure graphene (GRA) hexagonal boron nitride (hBN). We designed an hBN strip embedded in sheet. With such design, peptides can spontaneously linearly stretch out on stripe. By tuning strength external electric field, molecular transportation along stripe be effectively regulated. Subsequent motion applied record time-dependent signals. The signal features are then utilized train machine learning classification models. machine-learning-assisted recognition enables accurate determination protein's length. This nanoslit-sensing method may offer theoretical guidance 2D design peptide lengths protein-related diseases.
Language: Английский
Citations
0
Talanta, Journal Year: 2024, Volume and Issue: 285, P. 127402 - 127402
Published: Dec. 16, 2024
Language: Английский
Citations
0