Acta Physiologica,
Journal Year:
2015,
Volume and Issue:
216(1), P. 15 - 41
Published: May 25, 2015
Abstract
Skeletal
muscles
comprise
a
substantial
portion
of
whole
body
mass
and
are
integral
for
locomotion
metabolic
health.
Increasing
age
is
associated
with
declines
in
both
muscle
function
(e.g.
strength‐related
performance,
power)
quantitatively
outweighing
those
volume.
The
mechanisms
behind
these
multi‐faceted
involving
intrinsic
age‐related
dysregulation
environmental
influences
such
as
nutritional
physical
activity.
Ageing
degree
‘anabolic
resistance’
to
key
inputs,
which
likely
accelerates
the
processes
driving
ageing.
On
this
basis,
strategies
sensitize
and/or
promote
anabolic
responses
nutrition
activity
be
imperative
alleviating
progression
trajectory
sarcopenia.
Both
resistance‐
aerobic‐type
exercises
confer
functional
health
benefits
older
age,
clutch
research
suggests
that
enhancement
responsiveness
exercise
may
achieved
by
optimizing
modifications
muscle‐loading
paradigms
(workload,
volume,
blood
flow
restriction)
or
support
essential
amino
acid/leucine)
patterns.
Nonetheless,
more
work
needed
holistic
view
ageing
studies
taken
into
account.
This
should
include
improved
characterization
study
recruits,
activity/nutritional
behaviours,
limit
confounding
variables
influencing
whether
findings
attributable
other
influences.
on
balance,
partially
related
decline
aerobic
capacity.
There
also
good
evidence
flexibility
impaired
age.
Nature Communications,
Journal Year:
2015,
Volume and Issue:
6(1)
Published: Sept. 24, 2015
Abstract
Single-cell
analysis
reveals
aspects
of
cellular
physiology
not
evident
from
population-based
studies,
particularly
in
the
case
highly
multiplexed
methods
such
as
mass
cytometry
(CyTOF)
able
to
correlate
levels
multiple
signalling,
differentiation
and
cell
fate
markers.
Immunofluorescence
(IF)
microscopy
adds
information
on
morphology
microenvironment
that
are
obtained
using
flow-based
techniques,
but
multiplicity
conventional
IF
is
limited.
This
has
motivated
development
imaging
require
specialized
instrumentation,
exotic
reagents
or
proprietary
protocols
difficult
reproduce
most
laboratories.
Here
we
report
a
public-domain
method
for
achieving
high
single-cell
cyclic
immunofluorescence
(CycIF),
simple
versatile
procedure
which
four-colour
staining
alternates
with
chemical
inactivation
fluorophores
progressively
build
multichannel
image.
Because
CycIF
uses
standard
instrumentation
no
more
expensive
than
IF,
it
suitable
high-throughput
assays
screening
applications.
Cell Research,
Journal Year:
2018,
Volume and Issue:
28(3), P. 281 - 295
Published: Feb. 9, 2018
The
mitochondrial
network
is
not
only
required
for
the
production
of
energy,
essential
cofactors
and
amino
acids,
but
also
serves
as
a
signaling
hub
innate
immune
apoptotic
pathways.
Multiple
mechanisms
have
evolved
to
identify
combat
dysfunction
maintain
health
organism.
One
such
pathway
unfolded
protein
response
(UPR
Cells,
Journal Year:
2016,
Volume and Issue:
5(3), P. 34 - 34
Published: Aug. 18, 2016
Rab7
is
a
small
GTPase
that
belongs
to
the
Rab
family
and
controls
transport
late
endocytic
compartments
such
as
endosomes
lysosomes.
The
mechanism
of
action
in
pathway
has
been
extensively
studied.
fundamental
for
lysosomal
biogenesis,
positioning
functions,
trafficking
degradation
several
signaling
receptors,
thus
also
having
implications
on
signal
transduction.
Several
interacting
proteins
have
being
identified
leading
discovery
number
different
important
beside
its
established
role
endocytosis.
Furthermore,
specific
functions
neurons.
This
review
highlights
discusses
importance
cellular
pathways
processes.
