Hsa_circ_0042666 inhibits proliferation and invasion via regulating miR-223/TGFBR3 axis in laryngeal squamous cell carcinoma DOI Open Access
Zhenxing Wei,

Kunpeng Chang,

Chongsheng Fan

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2019, Volume and Issue: 119, P. 109365 - 109365

Published: Sept. 13, 2019

Circular RNAs (circRNAs) have been reported to play critical roles in tumorigenesis. However, the of circRNAs laryngeal squamous cell carcinoma (LSCC) are still largely unknown. In our present study, we identified a novel circRNA hsa_circ_0042666, which was poorly expressed LSCC. Low hsa_circ_0042666 expression closely associated with advanced tumor stage, lymph-node metastasis, and poor overall survival. vitro function assays, showed that dramatically reduced LSCC cells proliferation invasion vitro. mechanism, data indicated could competitively bind miR-223 as miRNA sponge regulate TGFBR3 progression. Altogether, these findings elucidated regulated by miR-223/TGFBR3 axis, might provide therapeutic strategy for treatment

Language: Английский

Exosomal circRNAs: biogenesis, effect and application in human diseases DOI Creative Commons

Yangxia Wang,

Jinbo Liu,

Junfen Ma

et al.

Molecular Cancer, Journal Year: 2019, Volume and Issue: 18(1)

Published: July 5, 2019

Exosomes have emerged as critical mediators of intercellular communication, both locally and systemically, by regulating a diverse range biological processes between cells. Circular RNA (circRNA) is novel member endogenous noncoding RNAs with widespread distribution cellular functions. Recently, circular been identified for their enrichment stability in exosomes. In this review, we outline the origin, biogenesis function exosomal circRNAs well roles various diseases. Although precise mechanisms gene regulation remain largely elusive, potential applications disease biomarkers therapeutic targets.

Language: Английский

Citations

540

Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes DOI
Huiwen Xiong, Zhipeng Huang,

Zhejun Yang

et al.

Small, Journal Year: 2021, Volume and Issue: 17(35)

Published: June 2, 2021

Exosomes, known as nanometer-sized vesicles (30-200 nm), are secreted by many types of cells. Cancer-derived exosomes have great potential to be biomarkers for early clinical diagnosis and evaluation cancer therapeutic efficacy. Conventional detection methods limited low sensitivity reproducibility. There hundreds papers published with different in recent years address these challenges. Therefore, this review, pioneering researches about various strategies comprehensively summarized the analytical performance tests is evaluated. Furthermore, exosome molecular composition (protein nucleic acid) profiling, a single their application reviewed. Finally, principles applications machine learning method presented.

Language: Английский

Citations

116

circRNAs and Exosomes: A Mysterious Frontier for Human Cancer DOI Creative Commons
Xuefei Shi, Bin Wang,

Xueren Feng

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2019, Volume and Issue: 19, P. 384 - 392

Published: Nov. 29, 2019

Exosomes are nano-sized membrane-bound vesicles and contain active substances (DNA, noncoding RNA [ncRNA], protein), which provide a novel method of transferring effector messages between cells. Circular RNAs (circRNAs), kind ncRNA, have attracted increasing attention over the last decade given advances in whole-genome transcriptome sequencing technologies. It has become increasingly clear that circRNAs regulate gene expression through various actions play diverse roles many fields human cancer biology. Notably, several studies reported enriched exosomes exosomal an important role Exosomal can be taken up by neighboring or distant cells affect aspects physiological pathological conditions recipient cells, potentially promoting cell communication tumor metastasis. Herein, we briefly review molecular mechanisms recent findings regarding circRNAs, highlight specific cancer.

Language: Английский

Citations

115

The potential role of exosomal circRNAs in the tumor microenvironment: insights into cancer diagnosis and therapy DOI Creative Commons
Juan Li, Guo Zhang, Chenguang Liu

et al.

Theranostics, Journal Year: 2021, Volume and Issue: 12(1), P. 87 - 104

Published: Nov. 10, 2021

Exosomes are multifunctional regulators of intercellular communication by carrying various messages under both physiological and pathological status cancer patients. Accumulating studies have identified the presence circular RNAs (circRNAs) in exosomes with crucial regulatory roles diverse pathophysiological processes. Exosomal circRNAs derived from donor cells can modulate crosstalk recipient locally or remotely to enhance development propagation, play tumor microenvironment (TME), leading significant enhancement immunity, metabolism, angiogenesis, drug resistance, epithelial mesenchymal transition (EMT), invasion metastasis. In this review, we describe advances exosomal their modulating hallmarks, especially those TME. Moreover, clinical application potential diagnosis therapy highlighted, bridging gap between basic knowledge practice.

Language: Английский

Citations

80

Circular RNAs in the tumour microenvironment DOI Creative Commons
Zhonghua Ma, You Shuai, Gao X

et al.

Molecular Cancer, Journal Year: 2020, Volume and Issue: 19(1)

Published: Jan. 14, 2020

Abstract Background Circular RNAs (circRNAs) are a new class of endogenous non-coding (ncRNAs) widely expressed in eukaryotic cells. Mounting evidence has highlighted circRNAs as critical regulators various tumours. More importantly, have been revealed to recruit and reprogram key components involved the tumour microenvironment (TME), mediate signaling pathways, thus affecting tumourigenesis, angiogenesis, immune response, metastatic progression. Main body abstract In this review, we briefly introduce biogenesis, characteristics classification circRNAs, describe mechanistic models circRNAs. Further, provide first systematic overview interplay between cellular/non-cellular counterparts TME highlight potential prospective biomarkers or targets cancer clinics. Finally, discuss biological mechanisms through which drive development resistance, revealing mystery drug resistance Short conclusion Deep understanding emerging role their involvements may therapeutic for patients. The combined targeting co-activated achieve higher efficiency become mode therapy future.

Language: Английский

Citations

79

Tumor Cells-derived exosomal CircRNAs: Novel cancer drivers, molecular mechanisms, and clinical opportunities DOI
Ali Vahabi, Jafar Rezaie, Mehdi Hassanpour

et al.

Biochemical Pharmacology, Journal Year: 2022, Volume and Issue: 200, P. 115038 - 115038

Published: April 13, 2022

Language: Английский

Citations

55

Roles and clinical application of exosomal circRNAs in the diagnosis and treatment of malignant tumors DOI Creative Commons
Dong Ye,

Mengdan Gong,

Yongqin Deng

et al.

Journal of Translational Medicine, Journal Year: 2022, Volume and Issue: 20(1)

Published: April 5, 2022

Abstract Exosomes are microvesicles secreted by cells. They contain a variety of bioactive substances with important roles in intercellular communication. Circular RNA (circRNA), type nucleic acid molecule found exosomes, forms covalently bonded closed loop without 5′ caps or 3′ poly(A) tails. It is structurally stable, widely distributed, and tissue specific. CircRNAs mainly act as microRNA sponges have regulatory gene expression; they superior to other non-coding RNAs molecular diagnostic markers drug treatment targets. Exosomal-derived circRNAs the body fluids tumor patients can modulate proliferation, invasion, metastasis, resistance. be used effective biomarkers for early non-invasive diagnosis prognostic evaluation tumors, also represent ideal targets precision therapeutic intervention. This review provides theoretical basis exploring applications exosomal malignant treatment. We describe biological functions occurrence development their potential utility treatment, possible mechanisms.

Language: Английский

Citations

44

CircCCT2/miR-146a-5p/IRAK1 axis promotes the development of head and neck squamous cell carcinoma DOI Creative Commons
Long He,

L L. Li,

Liting Zhao

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 14, 2025

Head and neck squamous cell carcinoma (HNSCC), a highly invasive malignancy with poor prognosis, is one of the most common cancers globally. Circular RNAs (circRNAs) have become key regulators human malignancies, but further studies are necessary to fully understand their functions possible causes in HNSCC. CircCCT2 expression levels HNSCC tissues cells were measured via qPCR. was characterized by Sanger sequencing, qRT-PCR, RNase R & Actinomycin D treatment, nucleoplasmic separation FISH experiments. CCK-8 colony formation assays performed determine proliferation, Transwell used migration invasion. A xenograft tumor model study influence circCCT2 on vivo. Dual-luciferase gene reporter, RIP, western blotting, rescue experiments, explore target-binding relationships regulatory mechanisms. significantly upregulated cells. High associated advanced T stage, N clinical stage prognosis. Functionally, we verified that promotes development vitro Mechanistically, functioning as competitive endogenous RNA (ceRNA) or miRNA sponge, binds directly miR-146a-5p increases interleukin-1 receptor-associated kinase 1 (IRAK1) levels, which enhances malignant driving epithelial-mesenchymal transition (EMT). through miR-146a-5p/IRAK1 axis, revealing potential biomarker target for

Language: Английский

Citations

1

Circular RNAs—The Road Less Traveled DOI Creative Commons
Ashirbad Guria,

Priyanka Sharma,

Sankar Natesan

et al.

Frontiers in Molecular Biosciences, Journal Year: 2020, Volume and Issue: 6

Published: Jan. 10, 2020

Circular RNAs are the recent addition in non-coding RNA family which has started gaining recognition after a decade of its non-existence. However, first few reports beginning this and accumulating evidences thereafter evoked researchers to navigate further quest for exploration circular RNAs. Joining 5' 3' ends molecules by backsplicing forms during co-transcriptional or post-transcriptional processes. These capable effectively sponging microRNAs, thereby regulates cellular processess, as evidenced from numerous animal plant systems. Preliminary studies have shown that imperative role transcriptional regulation, protein translation, possible therapeutic potential. High stability is rendered closed ends; nevertheless they prone degradation circulating endonucleases serum exosomes microRNA-mediated cleavage due high complementarity. identification using diverse methodologies delineating mechanism regulation molecular architecture given new direction research. In current review, we discussed biogenesis, structure, properties, degradation, growing detection methods system.

Language: Английский

Citations

67

The Biology and Function of Extracellular Vesicles in Cancer Development DOI Creative Commons
Xinyi Zhang, Dianfeng Liu, Yongjian Gao

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: Nov. 5, 2021

Extracellular vesicles (EVs) exert their biological functions by delivering proteins, metabolites, and nucleic acids to recipient cells. EVs play important roles in cancer development. The anti-tumor effect of is cargos carrying affect cell-to-cell communication. characteristics communication can potentially be applied for the therapy cancers, such as gastric cancer. In addition, used an effective deliver ncRNAs, peptides, drugs, target tumor tissues. have ability regulate cell apoptosis, autophagy, proliferation, migration ncRNA peptides that were engaged with associated signaling pathways This review focuses on composition, cargo, function, mechanism, application cancers.

Language: Английский

Citations

50