Investigating the PI3K/AKT/mTOR axis in Buzhong Yiqi Decoction’s anti-colorectal cancer activity DOI Creative Commons
Song Qiao, Xiaolong Li,

Shangzhen Yang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 10, 2025

Buzhong Yiqi Decoction (BZYQD) is a traditional Chinese medicine renowned for its anti-colorectal cancer (CRC) properties. However, the bioactive components and mechanisms of BZYQD against CRC remain unknown. In this study, LC-MS was used to analyze chemical composition BZYQD. Next, network pharmacology molecular docking investigate core targets CRC. Finally, we experimentally validated potential mechanism through in vitro studies. Our results identified 26 BZYQD; 75 "hithubs" were screened by pharmacology, mainly involving pathways such as including cancer, P13K-Akt signaling pathway, proteoglycans kaposi sarcoma-associated herpesvirus, lipid atherosclerosis pathways. Based on number key pathways, two most critical AKT1 PIK3CA selected. The component-target indicated that astragaloside IV, gancaonin A, quercetin, poricoic acid licoisoflavanone are anti-CRC Molecular showed strong binding affinity between these targets. phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway emerged primary target Further studies confirmed BZYQD's activity mediated PI3K/AKT/mTOR axis influences macrophage polarization. exerts therapeutic effects multiple components, targets, study elucidates effective treatment provides preliminary validation experimental

Language: Английский

PRM1201 effectively inhibits colorectal cancer metastasis via shaping gut microbiota and short- chain fatty acids DOI
Ru Jia,

Shiyun Shao,

Ping Zhang

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 132, P. 155795 - 155795

Published: June 3, 2024

Language: Английский

Citations

7
Host–microbe interaction-mediated resistance to DSS-induced inflammatory enteritis in sheep DOI Creative Commons

Shuo Yan,

Ruilin Du,

Wenna Yao

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Oct. 21, 2024

The disease resistance phenotype is closely related to immunomodulatory function and immune tolerance has far-reaching implications in animal husbandry human health. Microbes play an important role the initiation, prevention, treatment of diseases, but mechanisms host-microbiota interactions disease-resistant phenotypes are poorly understood. In this study, we hope uncover explain microbes intestinal diseases their action identify new potential treatments.

Language: Английский

Citations

7
Christensenella strain resources, genomic/metabolomic profiling, and association with host at species level DOI Creative Commons

Xin‐Wei Sun,

Haojie Huang, Xiaomeng Wang

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: May 9, 2024

The gut commensal bacteria

Language: Английский

Citations

6
Traditional Medicine Xianglian Pill Suppresses high-fat diet-related colorectal cancer via inactivating TLR4/MyD88 by remodeling gut microbiota composition and bile acid metabolism DOI
Chenxiao Ye,

Changhong Wu,

Yan Li

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 333, P. 118411 - 118411

Published: May 31, 2024

Language: Английский

Citations

6
Lung-intestinal axis, Shuangshen granules attenuate lung metastasis by regulating the intestinal microbiota and related metabolites DOI Creative Commons
Juan Li,

Bolun Shi,

Xiaoling Ren

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 132, P. 155831 - 155831

Published: June 15, 2024

Based on the proposed lung-intestinal axis, there is a significant correlation between microbiota and lung metastasis. Targeting microbial composition valuable in modulating host response to cancer therapeutics. As traditional Chinese medicine (TCM) formula, Shuangshen granules (SSG) are clinically useful delaying metastasis, but its underlying mechanisms remain unknown.

Language: Английский

Citations

4
Ginsenoside Re Prevents Depression-like Behaviors via Inhibition of Inflammation, Oxidative Stress, and Activating BDNF/TrkB/ERK/CREB Signaling: An In Vivo and In Vitro Study DOI
Hongyu Chen,

Mengmeng Dong,

Huihan He

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(36), P. 19838 - 19851

Published: Aug. 26, 2024

Depression is a widespread disease, with high mortality and recurrence rates. Recent studies have shown that elevated cytokine levels are implicated in the molecular mechanisms of depression. Oxidative stress contributes to stimulation production. Growing evidence suggests ginsenoside Re (Gs-Re) exerts neuroprotective effect on hippocampus by suppressing oxidative inflammation. However, mechanism Gs-Re treatment depression remain understudied. This study aimed evaluate antidepressant-like effects possible underlying mechanisms. In this article, was studied both vitro (H

Language: Английский

Citations

4
Kajf Alleviated Colorectal Cancer Liver Metastasis by Reshaping the Gut Microbiota and Inhibiting M2-Like Macrophage Polarization DOI
Zongmei Zheng, Yizhao Du, Hua Jiang

et al.

Published: Jan. 1, 2025

Background: Colorectal cancer liver metastasis (CRLM) is one of the most devastated complications colorectal (CRC) and usually indicates a poor prognosis for CRC. The herbal formula Kang-Ai-Jing-Fang (KAJF) has been applied in clinical against CRC several decades, but its underlying mechanism still undetermined.Purpose: study aims to explore anti-metastatic effect KAJF on CRLM by manipulating gut microbiota inhibiting M2-like macrophage activation.Methods: were orally administrated model mice induced intrasplenic injection murine cells. Fecal transplantation (FMT) was verify role KAJF-reshaped intervention's inhibition CRLM. Network pharmacology molecular docking conducted identify core targets active compounds. 16s rRNA, untargeted liquid chromatograph mass spectrometer (LC-MS/MS) metabolomics transcriptomics performed depict structure, metabolites profile differential genes.Results: effectively inhibited restored dysbiosis upregulating abundance butyrate-producing (Lactobacillus, Bacteroides, Bifidobacterium), further FMT validated therapeutic effects KAJF-remodeled bacteria delay enrichment. Additionally, net analysis revealed that ingredients from such as quercetin, luteolin, beta-sitosterol are highly interacted with TNF, AKT1 TP53, respectively. What' more, it observed population F4/80+ CD206+ macrophages related cytokines (CCL17, CCL22, IL10, IL4, TGF etc.) downregulated after treatment, well reduction CD4+ T cells myeloid-derived suppressor (MDSCs), an increasement CD8+ cells.Conclusions: research demonstrated suppressed partially regulating microbial metabolites, polarization.

Language: Английский

Citations

0
Shengjiang Xiexin Decoction ameliorates DSS-induced ulcerative colitis via activating Wnt/β-Catenin signaling to enhance epithelium renovation and modulating intestinal flora DOI
Chen Chen, Xiaohu Lin, Yimin Xie

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 139, P. 156456 - 156456

Published: Feb. 1, 2025

Language: Английский

Citations

0
Inhibition of Stemness and PD-L1 Expression by Pien Tze Huang Enhances T Cell-Mediated Killing of Colorectal Cancer DOI
Qin Lu, Zhuqing Zhang,

Sihan Liu

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119447 - 119447

Published: Feb. 1, 2025

Language: Английский

Citations

0
Chaihushugan powder regulates the gut microbiota to alleviate mitochondrial oxidative stress in the gastric tissues of rats with functional dyspepsia DOI Creative Commons
Xuejiao Liu,

Keming Yang,

Yuebo Jia

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 18, 2025

Functional dyspepsia (FD) is a prevalent functional gastrointestinal disorder associated with oxidative stress (OS) and dysbiosis. Chaihushugan powder (CHSGP) demonstrates efficacy in treating FD; however, the underlying therapeutic mechanism not yet elucidated. This study aims to investigate effects of CHSGP on OS gut microbiota (GM) FD rats, particular emphasis role GM as potential target for antioxidant properties CHSGP. The rat model was established modified tail-clamp stimulation administration decoction at dosage 9.6 g/kg via gavage duration 4 weeks. depleted by cocktail metronidazole (200 mg/kg), ampicillin neomycin sulfate vancomycin (100 mg/kg). Fecal transplantation (FMT) performed CHSGP-treated fecal supernatant 10 mL/kg. motility measured using rates gastric emptying small intestine propulsion. Hematoxylin eosin staining employed elucidate pathological changes, while transmission electron microscope used examine microstructures interstitial cells Cajal (ICC). Chemiluminescence, colorimetric assay, immunofluorescence co-staining, western blot assay were identify OS-related markers (ROS, SOD, NOX4, PRDX1, TRX2). Sequencing utilizing 16S rDNA. promoted motility, protected microstructure ICC, reduced rats. composition also regulated However, these disappeared after depletion. Fortunately, FMT therapy reinstated them. might regulate alleviate mitochondrial tissues

Language: Английский

Citations

0