Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 87 - 123
Published: Nov. 29, 2024
Language: Английский
Journal of Neuroimmune Pharmacology, Journal Year: 2025, Volume and Issue: 20(1)
Published: April 21, 2025
Abstract The pathophysiology of diabetes-induced brain injury involves pyroptosis, an inflammatory programmed cell death. This study aimed to investigate the potential protective effect cranberry extract (CE) against injury. Type 1 diabetes was induced by intraperitoneal injection streptozotocin in rats. Brain tissue samples were investigated for biochemical determination reduced glutathione (GSH), superoxide dismutase (SOD), and malondialdehyde (MDA), quantitative RT-PCR gene expression glial cell-derived neurotrophic factor (GDNF), lncRNA GAS-5, pyroptosis markers. ELISA used determine caspase-1 level immunohistochemical staining assessing IL-1β. Prophylactic dosing CE diabetic rats improved cognitive behavior significantly suppressed MDA concentration, genes (gasdermin D caspase 1), GAS-5. In addition, elevated GSH SOD activity, GDNF markedly IL-1β positive stained cells score brain. Phytochemical characterization FT-IR UPLC-PDA-MS/MS revealed cyanidin arabinoside, procyanidins, quercetin, isorhamnetin as key components. protects dysfunction targeting redox-related signaling pathways inducing anti-inflammatory effect. LncRNA GAS-5 downregulation pathway inhibition may contribute its beneficial effects, suggesting therapeutic potential.
Language: Английский
Citations
0
Computational Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 100355 - 100355
Published: May 1, 2025
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: March 29, 2024
Abstract Isorhamnetin is a natural flavonoid compound, rich in brass, alkaloids, and sterols with high medicinal value. This study investigated the effects of isorhamnetin on liver injury oxidative inflammatory responses heat-stroke-affected rats dry-heat environment. Fifty Sprague Dawley were randomly divided into five groups: normal temperature control (NC, saline), (DHC, low-dose isorhamnetin-pretreated (L-AS, 25 mg/Kg), medium-dose (M-AS, 50 high-dose (H-AS, 100 mg/Kg) group. Saline was administered to NC DHC groups corresponding concentrations remaining three for 1 week. Blood tissue analyzed stress inflammation. The histopathological score, serum enzyme (alanine transaminase, aspartate lactate dehydrogenase), index (superoxide dismutase [SOD], catalase [CAT], malondialdehyde), inflammation (tumor necrosis factor α [TNF-α], interleukin [IL]-1β, IL-6, lipopolysaccharides) significantly higher group than ( P < 0.05). These values L-AS, M-AS, H-AS lower those decreased an increase concentration 0.05), while CAT SOD showed opposite tendency expression nuclear kappa B (NF-κB), caspase-3, heat shock protein (HSP-70) Comparison between revealed that NF-кB caspase-3 decreased, HSP-70 continued difference significant among all 0.05); however, L-AS did not differ. In summary, has protective against rats. effect may be related its activities concerning antioxidative stress, anti-inflammatory response, inhibition expression, enhancement expression.
Language: Английский
Citations
2
Clinical Nutrition, Journal Year: 2024, Volume and Issue: 43(9), P. 1952 - 1971
Published: July 14, 2024
Parenteral nutrition (PN) is a life-saving procedure conducted to maintain proper nutritional state in patients with severe intestinal failure who cannot be fed orally. A serious complication of PN therapy liver failure, known as failure-associated disease (IFALD). The pathogenesis IFALD multifactorial and includes inhibition the farnesoid X receptor (FXR) by components, bacteria translocation from impaired intestines, intravenous line-associated bloodstream infection. Currently, most frequently researched therapeutic option for using lipid emulsions based on soy or fish oil and, therefore, free phytosterols FXR antagonists. Nevertheless, potential side effects lack soybean delivery seem outweigh benefits, especially pediatric population. admixture provides all necessary nutrients; however, it deprived exogenous natural bioactive compounds (NBCs) plant origin, such polyphenols, characterized health-promoting properties. Among them, many substances have already been demonstrate hepatoprotective effect various diseases. Therefore, searching new options among NBCs seems reasonable potentially successful. This review summarizes recent research polyphenols their use treating diseases, metabolic dysfunction-associated steatotic diseases (MASLD). Furthermore, scientific reports, we described molecular mechanism action selected that exert We also summarized current knowledge pathogenesis, undergoing clinical trials, presented future perspective therapy.
Language: Английский
Citations
2
RSC Advances, Journal Year: 2022, Volume and Issue: 12(54), P. 34971 - 34989
Published: Jan. 1, 2022
Taiju and Duoju are products of Hangbaiju (HJ) obtained during different collection periods, they have been commonly used as ingredients in tea beverages dietary traditional Chinese medicine. This study reports an integrated strategy based on metabolomics, bioinformatics molecular docking to further explore the effect harvesting period metabolic profile clinical efficacy HJ. Firstly, gas chromatography-mass spectrometry (GC-MS) ultra-high performance liquid chromatography-quadrupole time-of-flight mass (UHPLC-Q-TOF/MS) were employed for non-targeted metabolomics profiling essential oils flavonoids. A sequential window acquisition all theoretical fragment-ion spectra information-dependent (SWATH-IDA) bi-directionally verified (SIBDV) method was developed that integrates advantages both SWATH IDA characterizing Chemometric methods then screen potential chemical markers. Furthermore, HJ is effective hepatoprotective functions. Therefore, hepatocellular-carcinoma-related differentially expressed genes using bioinformatics, corresponding proteins molecularly docked with diagnostic In total, 78 volatile 63 flavonoids tentatively identified. The results allowed selection 11 metabolites (5 6 flavonoids), which nominated novel markers material authentication Duoju. Additionally, two associated hepatoma screened bioinformatics. All six flavonoid binding energies <-5 kcal mol-1 considered be anti-hepatoma biomarkers. Noticeably, Taiju, content hydroxygenkwanin showed a downward trend, but other five flavored difference compounds had upward trend. bestows unique flavor leading differences sensory aroma conclusion, transformation secondary dominant trend growth. These findings lay foundation food development distinguishing applications.
Language: Английский
Citations
8
Drug Research, Journal Year: 2024, Volume and Issue: 74(04), P. 156 - 163
Published: March 8, 2024
Abstract Diosgenin is a sapogenin with antidiabetic, antioxidant, and anti-inflammatory properties. The current study investigated whether diosgenin could ameliorate carbon tetrachloride (CCL4)-induced liver injury. To cause injury, CCL4 was injected intraperitoneally twice week for 8 weeks. Daily oral administration of at doses 20, 40, 80 mg/kg started one day before injection continued Finally, serum levels aspartate aminotransferase (AST), alanine (ALT), also albumin were assessed. Catalase superoxide dismutase (SOD) activities in addition to glutathione (GSH) malondialdehyde (MDA) quantified the homogenate routine histological evaluation conducted. Elevated enzymes decreased level caused by significantly restored following 40 mg/kg. Long-term increased inflammatory apoptotic factors such as IL-1β, caspase 3, TNF-α, IL-6 SOD catalase well GSH homogenates; while MDA increased. Treatment injured animals. In addition, MDA, or activity treatment. Additionally, aptly prevented aberrant changes. According obtained results, can dose-dependently diminish CCl4-induced functional deficits changes dose-dependent manner, possibly due its antioxidant anti-inflammation properties, beneficial effect comparable known hepatoprotective agent silymarin.
Language: Английский
Citations
1
Chemical Papers, Journal Year: 2024, Volume and Issue: 78(11), P. 6573 - 6584
Published: June 22, 2024
Language: Английский
Citations
1
Livers, Journal Year: 2022, Volume and Issue: 2(4), P. 425 - 435
Published: Dec. 13, 2022
Acetaminophen (APAP) is a widely used pain reliever that can cause liver injury or failure in response to an overdose. Understanding the mechanisms of APAP-induced cell death critical for identifying new therapeutic targets. In this respect it was hypothesized hepatocytes die by oncotic necrosis, apoptosis, necroptosis, ferroptosis and more recently pyroptosis. The latter characterized caspase-dependent gasdermin cleavage into C-terminal N-terminal fragment, which forms pores plasma membrane. release potassium, interleukin-1β (IL-1β), IL-18, other small molecules sublytic phase, be main function certain types such as inflammatory cells. Alternatively, process progress full lysis (pyroptosis) with extensive contents release. This review discusses experimental evidence involvement pyroptosis APAP hepatotoxicity well arguments against relevant mechanism hepatocytes. Based on evaluation currently available literature understanding pathophysiology, concluded pyroptotic unlikely contributor injury.
Language: Английский
Citations
7
Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 16, 2024
Language: Английский
Citations
1
Frontiers in Molecular Neuroscience, Journal Year: 2022, Volume and Issue: 15
Published: April 25, 2022
Glycine receptors (GlyRs) are the primary mediators of fast inhibitory transmission in mammalian spinal cord, where they modulate sensory and motor signaling. Mutations GlyR genes as well some other underlie hereditary disorder hyperekplexia, characterized by episodic muscle stiffness exaggerated startle responses. Here, we have investigated pain-related behavior expression cord deficient mutant mouse spastic (spa). In mice, number is reduced due to a β subunit gene (Glrb) mutation resulting aberrant splicing GlyRβ transcripts. Via direct physical interaction with anchoring protein gephyrin, this crucially involved postsynaptic clustering heteromeric GlyRs. We show that differentially affects aspects homozygous Glrbspa/Glrbspa mice. While response latencies noxious heat were unchanged, chemically induced revealed reduction licking time an increase flinching homozygotes during both phases formalin test. Mechanically nocifensive was although hind paw inflammation (by zymosan) resulted allodynia comparable wild-type Immunohistochemical staining massive dotted GlyRα immunoreactivity ventral dorsal horns, suggesting clustered at synaptic sites. Transcripts for all variants, however, not throughout horn These findings suggest loss functional subunits hence synaptically localized GlyRs compromises processing differentially, depending on stimulus modality.
Language: Английский
Citations
6