Amyloid Beta Peptides Lead to Mast Cell Activation in a Novel 3D Hydrogel Model DOI Open Access

Jingshu Liu,

Sihan Liu,

Li Zeng

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(15), P. 12002 - 12002

Published: July 26, 2023

Alzheimer's disease (AD) is a prevalent neurodegenerative and the world's primary cause of dementia among elderly population. The aggregation toxic amyloid-beta (Aβ) one main pathological hallmarks AD brain. Recently, neuroinflammation has been recognized as major features AD, which involves network interactions between immune cells. mast cell (MC) an innate type known to serve first responder changes crosstalk with microglia neurons. Although increased number cells were found near sites Aβ deposition, how are activated in not clear. We developed 3D culture system MCs investigated activation by peptides. Because collagen I component extracellular matrix (ECM) brain, we encapsulated human LADR gels formed I. that 3D-cultured survived proliferated at same level suspension. Additionally, they can be induced secrete inflammatory cytokines well MC proteases tryptase chymase typical activators interleukin 33 (IL-33) IgE/anti-IgE. Culturing peptides Aβ1-42, Aβ1-40, Aβ25-35 caused mediators, Aβ1-42 inducing maximum activation. These data indicate respond amyloid deposition elicit responses demonstrate validity gel model investigate environment understand AD.

Language: Английский

Adipokines: masterminds of metabolic inflammation DOI
Herbert Tilg, Gianluca Ianiro, Antonio Gasbarrini

et al.

Nature reviews. Immunology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Language: Английский

Citations

18
Mast cells in the autonomic nervous system and potential role in disorders with dysautonomia and neuroinflammation DOI Creative Commons
Theoharis C. Theoharides,

Assma Twahir,

Duraisamy Kempuraj

et al.

Annals of Allergy Asthma & Immunology, Journal Year: 2023, Volume and Issue: 132(4), P. 440 - 454

Published: Nov. 10, 2023

Mast cells (MC) are ubiquitous in the body, and they critical for not only allergic diseases but also immunity inflammation, including having potential involvement pathophysiology of dysautonomias neuroinflammatory disorders. MC located perivascularly close to nerve endings sites such as carotid bodies, heart, hypothalamus, pineal gland, adrenal gland that would allow them regulate be affected by autonomic nervous system (ANS). stimulated allergens many other triggers some from ANS can affect release neurosensitizing, proinflammatory, vasoactive mediators. Hence, may able homeostatic functions seem dysfunctional conditions, postural orthostatic tachycardia syndrome, autism spectrum disorder, myalgic encephalomyelitis/chronic fatigue Long-COVID syndrome. The evidence indicates there is a possible association between these conditions associated with activation. There no effective treatment any form than minimizing symptoms. Given ways could activated numerous mediators released, it important develop inhibit stimulation ANS-relevant

Language: Английский

Citations

16
Longitudinal analysis of the lung proteome reveals persistent repair months after mild to moderate COVID-19 DOI Creative Commons

S.M. Kanth,

J.A. Huapaya, Salina Gairhe

et al.

Cell Reports Medicine, Journal Year: 2024, Volume and Issue: 5(7), P. 101642 - 101642

Published: July 1, 2024

In order to assess homeostatic mechanisms in the lung after COVID-19, changes protein signature of bronchoalveolar lavage from 45 patients with mild moderate disease at three phases (acute, recovery, and convalescent) are evaluated over a year. During acute phase, inflamed uninflamed phenotypes characterized by expression tissue repair host defense response molecules. With inflammatory fibrogenic mediators decline clinical symptoms abate. However, 9 months, quantified radiographic abnormalities resolve majority patients, yet compared healthy persons, all showed ongoing activation cellular processes depression renin-kallikrein-kinin, coagulation, complement systems. This dissociation prolonged reparative symptom resolution suggests that occult disruption proteome is underrecognized may be relevant recovery other serious viral pneumonias.

Language: Английский

Citations

4
Amyloid Beta Peptides Lead to Mast Cell Activation in a Novel 3D Hydrogel Model DOI Open Access

Jingshu Liu,

Sihan Liu,

Li Zeng

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(15), P. 12002 - 12002

Published: July 26, 2023

Alzheimer's disease (AD) is a prevalent neurodegenerative and the world's primary cause of dementia among elderly population. The aggregation toxic amyloid-beta (Aβ) one main pathological hallmarks AD brain. Recently, neuroinflammation has been recognized as major features AD, which involves network interactions between immune cells. mast cell (MC) an innate type known to serve first responder changes crosstalk with microglia neurons. Although increased number cells were found near sites Aβ deposition, how are activated in not clear. We developed 3D culture system MCs investigated activation by peptides. Because collagen I component extracellular matrix (ECM) brain, we encapsulated human LADR gels formed I. that 3D-cultured survived proliferated at same level suspension. Additionally, they can be induced secrete inflammatory cytokines well MC proteases tryptase chymase typical activators interleukin 33 (IL-33) IgE/anti-IgE. Culturing peptides Aβ1-42, Aβ1-40, Aβ25-35 caused mediators, Aβ1-42 inducing maximum activation. These data indicate respond amyloid deposition elicit responses demonstrate validity gel model investigate environment understand AD.

Language: Английский

Citations

5