Aptamer–field-effect transistors overcome Debye length limitations for small-molecule sensing

Science, Journal Year: 2018, Volume and Issue: 362(6412), P. 319 - 324

Published: Sept. 6, 2018

Transistor sensing in salt solutions Molecular binding to receptors on the surface of field-effect transistors (FETs) can be sensed through changes transconductance. However, saline typically used with biomolecules create an electrical double layer that masks any events occur within about 1 nanometer from surface. Nakatsuka et al. overcame this limitation by using large, negatively charged DNA stem loop structures that, upon ligand binding, cause conformational FET, even high ionic strength. The authors demonstrate molecules such as dopamine artificial cerebrospinal fluid well neutral glucose and zwitterion like sphingosine-1-phosphate. Science , issue p. 319

Language: Английский

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Electrically-Transduced Chemical Sensors Based on Two-Dimensional Nanomaterials

Chemical Reviews, Journal Year: 2019, Volume and Issue: 119(1), P. 478 - 598

Published: Jan. 3, 2019

Electrically–transduced sensors, with their simplicity and compatibility standard electronic technologies, produce signals that can be efficiently acquired, processed, stored, analyzed. Two dimensional (2D) nanomaterials, including graphene, phosphorene (BP), transition metal dichalcogenides (TMDCs), others, have proven to attractive for the fabrication of high–performance electrically-transduced chemical sensors due remarkable physical properties originating from 2D structure. This review highlights advances in sensing rely on materials. The structural components such are described, underlying operating principles different types architectures discussed. features, properties, surface chemistry nanostructures dictate performance reviewed. Key application materials, both a historical analytical perspective, summarized four groups analytes: gases, volatile compounds, ions, biomolecules. is discussed context molecular design, structure–property relationships, device technology. outlook challenges opportunities nanomaterials future development also presented.

Language: Английский

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Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor

Nature Biomedical Engineering, Journal Year: 2019, Volume and Issue: 3(6), P. 427 - 437

Published: March 25, 2019

Language: Английский

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Solid-state nanopore sensors

Nature Reviews Materials, Journal Year: 2020, Volume and Issue: 5(12), P. 931 - 951

Published: Sept. 21, 2020

Language: Английский

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Detection principles of biological and chemical FET sensors

Biosensors and Bioelectronics, Journal Year: 2017, Volume and Issue: 98, P. 437 - 448

Published: July 5, 2017

The seminal importance of detecting ions and molecules for point-of-care tests has driven the search more sensitive, specific, robust sensors. Electronic detection holds promise future miniaturized in-situ applications can be integrated into existing electronic manufacturing processes technology. resulting small devices will inherently well suited multiplexed parallel detection. In this review, different field-effect transistor (FET) structures principles are discussed, including label-free indirect mechanisms. fundamental principle governing every potentiometric sensor is introduced, state-of-the-art FET reviewed. This followed by an analysis electrolyte interfaces their influence on operation. Finally, fundamentals mechanisms reviewed some schemes discussed. conclusion, current commercial efforts briefly considered.

Language: Английский

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Novel graphene-based biosensor for early detection of Zika virus infection

Biosensors and Bioelectronics, Journal Year: 2017, Volume and Issue: 100, P. 85 - 88

Published: Aug. 24, 2017

We have developed a cost-effective and portable graphene-enabled biosensor to detect Zika virus with highly specific immobilized monoclonal antibody. Field Effect Biosensing (FEB) antibodies covalently linked graphene enables real-time, quantitative detection of native viral (ZIKV) antigens. The percent change in capacitance response doses antigen (ZIKV NS1) coincides levels clinical significance buffer at concentrations as low 450 pM. Potential diagnostic applications were demonstrated by measuring simulated human serum. Selectivity was validated using Japanese Encephalitis NS1, homologous potentially cross-reactive antigen. Further, the platform can simultaneously provide advanced data nonclinical biophysical kinetics tools, making it adaptable both research possible applications. speed, sensitivity, selectivity this first-of-its-kind make an ideal candidate for development medical test.

Language: Английский

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Ultrasensitive detection of nucleic acids using deformed graphene channel field effect biosensors

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: March 24, 2020

Abstract Field-effect transistor (FET)-based biosensors allow label-free detection of biomolecules by measuring their intrinsic charges. The limit these sensors is determined the Debye screening charges from counter ions in solutions. Here, we use FETs with a deformed monolayer graphene channel for nucleic acids. These devices even millimeter scale channels show an ultra-high sensitivity buffer and human serum sample down to 600 zM 20 aM, respectively, which are ∼18 ∼600 acid molecules. Computational simulations reveal that nanoscale deformations can form ‘electrical hot spots’ sensing reduce charge at concave regions. Moreover, could exhibit band-gap, allowing exponential change source-drain current small numbers Collectively, phenomena ultrasensitive electronic biomolecular structures.

Language: Английский

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Synthesis, Assembly, and Applications of Hybrid Nanostructures for Biosensing

Chemical Reviews, Journal Year: 2017, Volume and Issue: 117(20), P. 12942 - 13038

Published: Sept. 13, 2017

The robust, sensitive, and selective detection of targeted biomolecules in their native environment by prospective nanostructures holds much promise for real-time, accurate, high throughput biosensing. However, order to be competitive, current biosensor nanotechnologies need significant improvements, especially specificity, integration, rate, long-term stability complex bioenvironments. Advancing biosensing chemically "noisy" bioenvironments require careful engineering nanoscale components that are highly biorecognition ligands capable exquisite binding, seamless integration at a level devices have yet achieve. This review summarizes recent advances the synthesis, assembly, applications nanoengineered reporting transducing critical efficient First, major classes nanostructured components, both inorganic reporters organic transducers, discussed context synthetic control individual compositions, shapes, properties. Second, design surface functionalities path, characterization interfacial architectures, multiple into multifunctional ordered extensively examined. Third, examples structures created from hybrid nanomaterials reviewed, with distinct emphasis on tailor nanosensor designs specific operating environments. Finally, we offer perspective future developments nanohybrid materials nanosensors, outline possible directions pursued may yield breakthrough results, envision exciting potential high-performance will cause disruptive improvements field

Language: Английский

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Biosensing based on field-effect transistors (FET): Recent progress and challenges

TrAC Trends in Analytical Chemistry, Journal Year: 2020, Volume and Issue: 133, P. 116067 - 116067

Published: Oct. 9, 2020

Language: Английский

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Highly Sensitive and Multiplexed Protein Measurements

Chemical Reviews, Journal Year: 2018, Volume and Issue: 119(1), P. 293 - 321

Published: Aug. 28, 2018

Proteins are involved in many biological processes. Misfolded, truncated, or mutated proteins as well over- underexpressed have been implicated diseases. Therefore, detection and quantification of is extremely important. Conventional techniques such the enzyme-linked immunosorbent assay, Western Blot, mass spectrometry enabled discovery study samples. However, important present at low concentrations, rendering them undetectable using conventional techniques. Furthermore, limited ability to simultaneously measure multiple a sample has constrained our fully proteome. In this review, we comprehensively discuss approaches for protein detection. We first fundamentals assays, including affinity reagents, surface functionalization, assay formats, signal detection, multiplexing. then challenges with these methods review existing highly sensitive multiplexed Finally, recent advances from literature future directions.

Language: Английский

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