Biochimie, Journal Year: 2020, Volume and Issue: 178, P. 39 - 48
Published: Aug. 13, 2020
Language: Английский
PLoS Genetics, Journal Year: 2019, Volume and Issue: 15(8), P. e1008288 - e1008288
Published: Aug. 8, 2019
Neuronal physiology is particularly sensitive to acute stressors that affect excitability, many of which can trigger seizures and epilepsies. Although intrinsic neuronal homeostasis plays an important role in maintaining overall nervous system robustness its resistance stressors, the specific genetic molecular mechanisms underlie these processes are not well understood. Here we used a reverse approach Drosophila test hypothesis voltage-gated ion channels contribute homeostasis, robustness, stress resistance. We found activity potassium channel seizure (sei), ortholog mammalian ERG family, essential for protecting flies from heat-induced seizures. sei broadly expressed system, our data indicate impact on organismal environmental primarily mediated via action excitatory neurons, octopaminergic as neuropile ensheathing perineurial glia. Furthermore, studies suggest human mutations (hERG), have been implicated cardiac Long QT Syndrome (LQTS), may also high incidence LQTS patients cardiovascular-independent neurogenic pathway.
Language: Английский
Citations
20
Epilepsia, Journal Year: 2022, Volume and Issue: 63(10), P. 2543 - 2560
Published: July 29, 2022
The switch between nonseizure and seizure states involves profound alterations in network excitability synchrony. In this study, we aimed to identify compare features of neural dynamics across multiple zebrafish epilepsy models.Inspired by video-electroencephalographic recordings patients, developed a framework study spontaneous photically evoked locomotor activity larvae, combining high-throughput behavioral tracking whole-brain vivo two-photon calcium imaging.Our setup allowed us dissect physiological that are divergent or convergent models. We observed exhibit great diversity Nonetheless, during photic stimulation, hyperexcitability rapid response were well conserved models, highlighting the reliability for assays. Intriguingly, several initial elevated is often followed decay prominent depressed state. Elevated following state seizure-prone networks significantly reduced antiseizure medication valproic acid. Finally, depression stimulation temporally overlap with slow recruitment astroglial signals enhanced networks.We argue fast likely due homeostatic mechanisms triggered excessive activity. An improved understanding interplay might suggest tailored therapies.
Language: Английский
Citations
11
Current Opinion in Neurobiology, Journal Year: 2023, Volume and Issue: 80, P. 102702 - 102702
Published: March 23, 2023
Language: Английский
Citations
6
National Science Review, Journal Year: 2022, Volume and Issue: 9(12)
Published: July 27, 2022
Abstract While endogenous lipids are known to exhibit rhythmic oscillations, less is about how specific modulate circadian behavior. Through a series of loss-of-function and gain-of-function experiments on ceramide phosphoethanolamine (CPE) synthase Drosophila, we demonstrated that pan-glial-specific deficiency in membrane CPE, the structural analog mammalian sphingomyelin (SM), leads arrhythmic locomotor behavior shortens lifespan, while reverse true for increasing CPE. Comparative proteomics uncovered dysregulated synaptic glutamate utilization transport CPE-deficient flies. An extensive genetic screen was conducted verify role differentially expressed proteins regulation. Arrhythmic locomotion under cpes1 mutant background rescued only by restoring CPE or SM through expressing their respective synthases. Our results underscore essential maintaining homeostasis modulating Drosophila. The findings suggest region-specific elevations functional can benefit
Language: Английский
Citations
10
PLoS Biology, Journal Year: 2022, Volume and Issue: 20(9), P. e3001599 - e3001599
Published: Sept. 28, 2022
Cell division, wherein 1 cell divides into 2 daughter cells, is fundamental to all living organisms. Cytokinesis, the final step in begins with formation of an actomyosin contractile ring, positioned midway between segregated chromosomes. Constriction ring concomitant membrane deposition a specified spatiotemporal manner generates cleavage furrow that physically separates cytoplasm. Unique lipids specific biophysical properties have been shown localize intercellular bridges (also called midbody) connecting dividing cells; however, their biological roles and delivery mechanisms remain largely unknown. In this study, we show ceramide phosphoethanolamine (CPE), structural analog sphingomyelin, has unique acyl chain anchors Drosophila spermatocytes essential for meiotic cytokinesis. The head group CPE also important spermatogenesis. We find aberrant central spindle behavior but not mislocalization phosphatidylinositol phosphates (PIPs) at plasma responsible male cytokinesis defect CPE-deficient animals. Further, demonstrate enrichment multivesicular bodies marked by Rab7, which turn furrow. Volume electron microscopy analysis using correlative light focused ion beam scanning shows CPE-enriched Rab7 positive endosomes are juxtaposed on material. Correlative transmission reveal as body-like organelle releases its intraluminal vesicles vicinity ingressing furrows. Genetic ablation or Rab35 expression dominant negative Rab11 results significant defects. function required localization Our imply endosomal membranes crucial
Language: Английский
Citations
9
Communications Biology, Journal Year: 2019, Volume and Issue: 2(1)
Published: July 3, 2019
Abstract Although numerous studies have demonstrated that neuronal mechanisms regulate alcohol-related behaviors, very few investigated the direct role of glia in behavioral responses to alcohol. The results described here begin fill this gap alcohol behavior and gliobiology fields. Since Drosophila exhibit conserved their CNS are similar mammalian glia, we used exploring behavior. We found knockdown Cysteine proteinase-1 ( Cp1 ) increased sedation effect was specific cortex adulthood. These data implicate behaviors. Cortex functionally homologous astrocytes is orthologous Cathepsin L. Our raise possibility cathepsins may influence mammals via roles astrocytes.
Language: Английский
Citations
13
Metabolomics, Journal Year: 2020, Volume and Issue: 16(10)
Published: Oct. 1, 2020
Language: Английский
Citations
11
Metabolites, Journal Year: 2021, Volume and Issue: 11(12), P. 862 - 862
Published: Dec. 11, 2021
Ports are a good example of how coastal environments, gathering set diverse ecosystems, subjected to pollution factors coming from human activities both on land and at sea. Among them, trace element as copper represents major factor. Abundant in port ecosystem, is transported by runoff water results features (corrosion structures, fuel, anti-fouling products, etc.). The variegated scallop Mimachlamys varia common the Atlantic areas likely be directly influenced pollution, due its sessile filtering lifestyle. Thus, aim present study investigate disruption metabolism, under short exposure (48 h) concentration frequently encountered waters largest marina Europe (82 μg/L). For this, we chose non-targeted metabolomic approach using ultra-high performance liquid chromatography coupled high resolution mass spectrometry (UHPLC-HRMS), offering level sensitivity allowing without priori entire metabolome. We described 28 metabolites clearly modulated copper. They reflected action several biological functions such osmoregulation, oxidative stress, reproduction energy metabolism.
Language: Английский
Citations
10
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 2, 2024
Dihydroceramide desaturases convert dihydroceramides to ceramides, the precursors of all complex sphingolipids. Reduction DEGS1 dihydroceramide desaturase function causes pediatric neurodegenerative disorder hypomyelinating leukodystrophy-18 (HLD-18). We discovered that infertile crescent (ifc), Drosophila homolog, is expressed primarily in glial cells promote CNS development by guarding against neurodegeneration. Loss ifc massive accumulation and severe morphological defects cortex glia, including endoplasmic reticulum (ER) expansion, failure neuronal ensheathment, lipid droplet depletion. RNAi knockdown upstream ceramide synthase schlank glia mutants rescues ER suggesting drives this phenotype. but not neurons cell death, promotes survival. Our work identifies as primary site disease progression HLD-18 may inform on juvenile forms ALS, which also feature elevated levels.
Language: Английский
Citations
1
Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)
Published: March 18, 2024
Video Abstract.
Language: Английский
Citations
1