Current Biology,
Journal Year:
2022,
Volume and Issue:
32(18), P. 4025 - 4039.e3
Published: Aug. 18, 2022
The
maturation
of
sleep
behavior
across
a
lifespan
(sleep
ontogeny)
is
an
evolutionarily
conserved
phenomenon.
Mammalian
studies
have
shown
that
in
addition
to
increased
duration,
early
life
exhibits
stark
differences
compared
with
mature
regard
states.
How
the
intrinsic
output
circuits
contributes
ontogeny
poorly
understood.
fruit
fly
Drosophila
melanogaster
multifaceted
changes
from
juvenile
adulthood.
Here,
we
use
non-invasive
probabilistic
approach
investigate
architecture
and
flies.
Increased
flies
driven
primarily
by
decreased
probability
transitioning
wake
characterized
more
time
deeper
Functional
manipulations
sleep-promoting
neurons
dorsal
fan-shaped
body
(dFB)
suggest
these
differentially
regulate
Transcriptomic
analysis
dFB
at
different
ages
subsequent
RNAi
screen
implicate
genes
involved
circuit
maturation.
These
results
reveal
dynamic
transcriptional
states
contribute
lifespan.
The
central
complex
(CX)
plays
a
key
role
in
many
higher-order
functions
of
the
insect
brain
including
navigation
and
activity
regulation.
Genetic
tools
for
manipulating
individual
cell
types,
knowledge
what
neurotransmitters
neuromodulators
they
express,
will
be
required
to
gain
mechanistic
understanding
how
these
are
implemented.
We
generated
characterized
split-GAL4
driver
lines
that
express
or
small
subsets
about
half
CX
types.
surveyed
neuropeptide
receptor
expression
using
fluorescent
situ
hybridization.
About
neuropeptides
we
examined
were
expressed
only
few
cells,
while
rest
dozens
hundreds
cells.
Neuropeptide
receptors
more
broadly
at
lower
levels.
Using
our
GAL4
drivers
mark
found
51
85
types
least
one
21
multiple
neuropeptides.
Surprisingly,
all
co-expressed
molecule
neurotransmitter.
Finally,
used
identify
whose
activation
affects
sleep,
identified
other
link
circadian
clock
CX.
well-characterized
genetic
information
on
neurotransmitter
provide
should
enhance
studies
Nature Neuroscience,
Journal Year:
2024,
Volume and Issue:
27(2), P. 359 - 372
Published: Jan. 23, 2024
The
sleep-wake
cycle
is
determined
by
circadian
and
sleep
homeostatic
processes.
However,
the
molecular
impact
of
these
processes
their
interaction
in
different
brain
cell
populations
are
unknown.
To
fill
this
gap,
we
profiled
single-cell
transcriptome
adult
Drosophila
brains
across
four
times.
We
show
type-specific
transcriptomic
changes,
with
glia
displaying
largest
variation.
Glia
also
among
few
types
whose
gene
expression
correlates
both
homeostat
clock.
drive
level
affect
clock
regulators
glia,
disrupting
genes
specifically
impairs
rebound
after
deprivation.
These
findings
provide
a
comprehensive
view
effects
on
distinct
an
entire
animal
reveal
as
site
two
to
determine
dynamics.
Science Advances,
Journal Year:
2024,
Volume and Issue:
10(8)
Published: Feb. 21, 2024
Identifying
different
sleep
stages
in
humans
and
other
mammals
has
traditionally
relied
on
electroencephalograms.
Such
an
approach
is
not
feasible
certain
animals
such
as
invertebrates,
although
these
could
also
be
sleeping
stages.
Here,
we
perform
long-term
multichannel
local
field
potential
recordings
the
brains
of
behaving
flies
undergoing
spontaneous
bouts.
We
acquired
consistent
spatial
potentials
across
multiple
flies,
allowing
us
to
compare
brain
activity
awake
periods.
Using
machine
learning,
uncover
distinct
temporal
explore
associated
spectral
features
fly
brain.
Further,
analyze
electrophysiological
correlates
microbehaviors
with
confirm
existence
a
stage
rhythmic
proboscis
extensions
show
that
this
sleep-related
behavior
differ
significantly
from
those
same
during
wakefulness,
indicating
dissociation
between
states
wherein
behaviors
reside.
The
central
complex
(CX)
plays
a
key
role
in
many
higher-order
functions
of
the
insect
brain
including
navigation
and
activity
regulation.
Genetic
tools
for
manipulating
individual
cell
types,
knowledge
what
neurotransmitters
neuromodulators
they
express,
will
be
required
to
gain
mechanistic
understanding
how
these
are
implemented.
We
generated
characterized
split-GAL4
driver
lines
that
express
or
small
subsets
about
half
CX
types.
surveyed
neuropeptide
receptor
expression
using
fluorescent
situ
hybridization.
About
neuropeptides
we
examined
were
expressed
only
few
cells,
while
rest
dozens
hundreds
cells.
Neuropeptide
receptors
more
broadly
at
lower
levels.
Using
our
GAL4
drivers
mark
found
51
85
types
least
one
21
multiple
neuropeptides.
Surprisingly,
all
co-expressed
neurotransmitter.
Finally,
used
identify
whose
activation
affects
sleep,
identified
other
link
circadian
clock
CX.
well-characterized
genetic
information
on
neurotransmitter
provide
should
enhance
studies
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: June 14, 2024
Abstract
Living
organisms
synchronize
their
biological
activities
with
the
earth’s
rotation
through
circadian
clock,
a
molecular
mechanism
that
regulates
biology
and
behavior
daily.
This
synchronization
factually
maximizes
positive
(e.g.,
social
interactions,
feeding)
during
safe
periods,
minimizes
exposure
to
dangers
predation,
darkness)
typically
at
night.
Beyond
basic
regulation,
some
behaviors
like
sleep
have
an
additional
layer
of
homeostatic
control,
ensuring
those
essential
are
fulfilled.
While
is
predominantly
governed
by
secondary
regulator,
though
not
well-understood,
ensures
adherence
necessary
amounts
hints
fundamental
function
beyond
simple
energy
conservation
safety.
Here
we
explore
regulation
across
seven
Drosophila
species
diverse
ecological
niches,
revealing
while
circadian-driven
aspects
consistent,
varies
significantly.
The
findings
suggest
in
Drosophilids,
evolved
primarily
for
purposes.
more
complex,
homeostatically
regulated
functions
appear
independently
species-specific
manner,
universally
conserved.
laboratory
model
may
reproduce
recapitulate
primordial
evolution.
