Spatiotemporal control of root immune responses during microbial colonization DOI Creative Commons
Huei‐Hsuan Tsai, Jiachang Wang, Niko Geldner

et al.

Current Opinion in Plant Biology, Journal Year: 2023, Volume and Issue: 74, P. 102369 - 102369

Published: May 2, 2023

The entire evolutionary trajectory of plants towards large and complex multi-cellular organisms has been accompanied by incessant interactions with omnipresent unicellular microbes. This led to the evolution highly microbial communities, whose members display spectrum pathogenic mutualistic behaviors. Plant roots are dynamic, fractally growing organs even small Arabidopsis harbor millions individual microbes diverse taxa. It is evident that at different positions on a root surface could experience fundamentally environments, which, moreover, rapidly change over time. Differences in spatial scales between compares humans cities they inhabit. Such considerations make it mechanisms root-microbe can only be understood if analyzed relevant temporal scales. review attempts provide an overview rapid recent progress made mapping manipulating plant damage immune responses cellular resolution, as well visualizing bacterial communities their transcriptional activities. We further discuss impact such approaches will have for more predictive understanding interactions.

Language: Английский

Commensal lifestyle regulated by a negative feedback loop between Arabidopsis ROS and the bacterial T2SS DOI Creative Commons
Frederickson Entila, Xiaowei Han, Akira Mine

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 11, 2024

Despite the plant health-promoting effects of microbiota, these assemblages also comprise potentially detrimental microbes. How immunity controls its microbiota to promote health under conditions remains largely unknown. We find that commensal bacteria isolated from healthy Arabidopsis plants trigger diverse patterns reactive oxygen species (ROS) production dependent on immune receptors and completely NADPH oxidase RBOHD selectively inhibited specific commensals, notably Xanthomonas L148. Through random mutagenesis, we L148 gspE, encoding a type II secretion system (T2SS) component, is required for damaging rbohD mutant plants. In planta bacterial transcriptomics reveals suppresses most T2SS gene expression including gspE. colonization protected against pathogen, when gspE was by ROS or mutation. Thus, negative feedback loop between tames leaf turns it into microbe beneficial host.

Language: Английский

Citations

32
Nonpathogenic Pseudomonas syringae derivatives and its metabolites trigger the plant “cry for help” response to assemble disease suppressing and growth promoting rhizomicrobiome DOI Creative Commons
Yunpeng Liu, Huihui Zhang, Jing Wang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 1, 2024

Abstract Plants are capable of assembling beneficial rhizomicrobiomes through a “cry for help” mechanism upon pathogen infestation; however, it remains unknown whether we can use nonpathogenic strains to induce plants assemble rhizomicrobiome against invasion. Here, used series derivatives Pseudomonas syringae pv. tomato DC3000 elicit different levels the immune response Arabidopsis and revealed that two induced soil-borne legacy, demonstrating similar triggering effect as wild-type DC3000. In addition, an increase in abundance Devosia rhizosphere by decreased root exudation myristic acid was confirmed be responsible growth promotion disease suppression legacy. Furthermore, could heat-killed flg22 blocked effector triggered immunity (ETI) -eliciting derivative conclusion, demonstrate potential bacteria bacterial elicitors promote generation disease-suppressive soils.

Language: Английский

Citations

30
Exploring the potential of endophyte-plant interactions for improving crop sustainable yields in a changing climate DOI Creative Commons

Lorenzo Sena,

E. Mica, Giampiero Valé

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: March 20, 2024

Climate change poses a major threat to global food security, significantly reducing crop yields as cause of abiotic stresses, and for boosting the spread new old pathogens pests. Sustainable management route mitigation challenge recruiting an array solutions tools aims. Among these, deployment positive interactions between micro-biotic components agroecosystems plants can play highly significant role, part agro-ecological revolution. Endophytic microorganisms have emerged promising solution tackle this challenge. Arbuscular Mycorrhizal Fungi (AMF) endophytic bacteria fungi demonstrated their potential alleviate stresses such drought heat stress, well impacts biotic stresses. They enhance in sustainable way also by other mechanisms, improving nutrient uptake, or direct effects on plant physiology. In review we summarize update main types endophytes, highlight several studies that demonstrate efficacy explore possible avenues implementing crop-microbiota interactions. The mechanisms underlying these are complex require comprehensive understanding. For reason, omic technologies genomics, transcriptomics, proteomics, metabolomics been employed unravel, higher level information, network microorganisms. Therefore, discuss various approaches techniques used so far study plant-endophyte

Language: Английский

Citations

21
The plant microbiota: composition, functions, and engineering DOI
Matteo Chialva, Luisa Lanfranco, Paola Bonfante

et al.

Current Opinion in Biotechnology, Journal Year: 2021, Volume and Issue: 73, P. 135 - 142

Published: Aug. 12, 2021

Language: Английский

Citations

94
Sculpting the soil microbiota DOI
Valéria Custódio, Mathieu Gonin, Georg Stabl

et al.

The Plant Journal, Journal Year: 2021, Volume and Issue: 109(3), P. 508 - 522

Published: Nov. 7, 2021

Soil is a living ecosystem, the health of which depends on fine interactions among its abiotic and biotic components. These form delicate equilibrium maintained through multilayer network that absorbs certain perturbations guarantees soil functioning. Deciphering principles governing within soils critical importance for their management conservation. Here, we focus microbiota discuss complexity impact composition function interaction with plants. We how physical aspects influence microbiota-plant support plant growth responses to nutrient deficiencies. predict understanding determining configuration functioning will contribute design microbiota-based strategies preserve natural resources develop more environmentally friendly agricultural practices.

Language: Английский

Citations

64
Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots DOI Creative Commons

Katarzyna W. Wolinska,

Nathan Vannier,

Thorsten Thiergart

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2021, Volume and Issue: 118(49)

Published: Dec. 1, 2021

Significance Understanding how host–microbe homeostasis is controlled and maintained in plant roots key to enhance productivity. However, the factors that contribute maintenance of this equilibrium between their multikingdom microbial communities remain largely unknown. Here, we observed a link fungal load health, showed modulation abundance tightly by two-layer regulatory circuit involving host innate immune system on one hand bacterial root commensals another hand. Our results shed light into microbe–microbe interactions act concert prevent dysbiosis Arabidopsis thaliana roots, thereby promoting health maintaining growth-promoting activities commensals.

Language: Английский

Citations

60
Transcriptomic and Metabolomic Approaches Deepen Our Knowledge of Plant–Endophyte Interactions DOI Creative Commons
Xueliang Chen,

Sun Mei-Chen,

Sun‐Li Chong

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 12

Published: Jan. 27, 2022

In natural systems, plant-symbiont-pathogen interactions play important roles in mitigating abiotic and biotic stresses plants. Symbionts have their own special recognition ways, but they may share some similar characteristics with pathogens based on studies of model microbes Multi-omics technologies could be applied to study plant-microbe interactions, especially plant-endophyte interactions. Endophytes are naturally occurring that inhabit plants, do not cause apparent symptoms them, arise as an advantageous source novel metabolites, agriculturally promoters, stress resisters host Although biochemical, physiological, molecular investigations demonstrated endophytes confer benefits hosts, terms promoting plant growth, increasing metabolic capabilities, enhancing resistance, consist complex mechanisms between the two symbionts. Further knowledge these gained by adopting a multi-omics approach. The involved interaction, which can range from colonization protection against adverse conditions, has been investigated transcriptomics metabolomics. This review aims provide effective means ways applying solve current problems characterization involving colonization. obtained results should useful for identifying key determinants such would also timely theoretical material basis interaction applications.

Language: Английский

Citations

54
The Use of Synthetic Microbial Communities to Improve Plant Health DOI
Samuel J. Martins,

Josephine Pasche,

Hiago Antonio Oliveira da Silva

et al.

Phytopathology, Journal Year: 2023, Volume and Issue: 113(8), P. 1369 - 1379

Published: March 1, 2023

Despite the numerous benefits plants receive from probiotics, maintaining consistent results across applications is still a challenge. Cultivation-independent methods associated with reduced sequencing costs have considerably improved overall understanding of microbial ecology in plant environment. As result, now, it possible to engineer consortium microbes aiming for health. Such synthetic communities (SynComs) contain carefully chosen species produce desired microbiome function. Microbial biofilm formation, production secondary metabolites, and ability induce resistance are some traits consider when designing SynComs. Plant-associated not assembled randomly. Ecological theories suggest that these defined phylogenetic organization structured by general community assembly rules. Using machine learning, we can study rules target functions generate phenotypes. Well-structured assemblages more likely lead stable SynCom thrives under environmental stressors as compared classical selection single activities or taxonomy. However, ensuring colonization long-term phenotype stability one challenges overcome SynComs, may change over time horizontal gene transfer retained mutations. Here, explored advances made research regarding health, focusing on bacteria, they most dominant form other members commonly found studies.

Language: Английский

Citations

37
Volatile methyl jasmonate from roots triggers host-beneficial soil microbiome biofilms DOI Creative Commons
Omkar Kulkarni, Mrinmoy Mazumder,

Shruthi Kini

et al.

Nature Chemical Biology, Journal Year: 2023, Volume and Issue: 20(4), P. 473 - 483

Published: Nov. 13, 2023

Abstract The rhizosphere is a niche surrounding plant roots, where soluble and volatile molecules mediate signaling between plants the associated microbiota. preferred lifestyle of soil microorganisms in form biofilms. However, less known about whether root organic compounds (rVOCs) can influence biofilms beyond 2–10 mm zone influenced by exudates. We report that rVOCs shift microbiome composition growth dynamics complex This evolutionarily conserved from ferns to higher plants. Methyl jasmonate (MeJA) bioactive signal rapidly triggers both biofilm changes. In contrast planktonic community, resulting community provides ecological benefits host distance via enhancement. Thus, defense signal, MeJA, co-opted for assembling host-beneficial microbiota extending sphere rhizosphere.

Language: Английский

Citations

31
The need for an integrated multi‐OMICs approach in microbiome science in the food system DOI Creative Commons
Ilario Ferrocino, Kalliopi Rantsiou, Ryan McClure

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2023, Volume and Issue: 22(2), P. 1082 - 1103

Published: Jan. 12, 2023

Microbiome science as an interdisciplinary research field has evolved rapidly over the past two decades, becoming a popular topic not only in scientific community and among general public, but also food industry due to growing demand for microbiome-based technologies that provide added-value solutions. expanded context of systems, strongly driven by methodological advances different -omics fields leverage our understanding microbial diversity function. However, managing integrating complex layers are still challenging. Within Coordinated Support Action MicrobiomeSupport (https://www.microbiomesupport.eu/), project supported European Commission, workshop "Metagenomics, Metaproteomics Metabolomics: need data integration microbiome research" gathered 70 participants from relevant discuss challenges promote switch descriptive studies functional studies, elucidating biology interactive roles microbiomes systems. A combination is proposed. This will reduce biases resulting each individual technology result more comprehensive view biological system whole. Although combinations datasets rare, advanced bioinformatics tools artificial intelligence approaches can contribute understanding, prediction, management microbiome, thereby providing basis improvement quality safety.

Language: Английский

Citations

30