Nematode homologs of the sour taste receptor Otopetrin1 are evolutionarily conserved acid-sensitive proton channels DOI Creative Commons
Shitian Li, Umar Al-Sheikh, Yili Chen

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Jan. 26, 2023

Numerous taste receptors and related molecules have been identified in vertebrates invertebrates. Otopetrin1 has recently as mammalian sour receptor which is essential for acid sensation. However, whether other Otopetrin proteins are involved PH-sensing remains unknown. In C. elegans, there eight otopetrin homologous genes but their expression patterns functions not reported so far. Through heterologous HEK293T cells, we found that ceOTOP1a can be activated by NMDG+ solution without conventional cations, generated inward currents blocked zinc ions. Moreover, channels widely expressed numerous tissues, especially sensory neurons the nematode. These results suggest biophysical characteristics of nematodes generally conserved. a series single gene mutations otopetrins, were constructed CRISPR-Cas9 method, did affect either calcium responses ASH polymodal to stimulation or avoidance behaviors, suggesting might diverse among species. This study reveals nematode Otopetrins evolutionarily conserved acid-sensitive proton channels, provides framework further revealing function mechanisms both invertebrates vertebrates.

Language: Английский

Alkaline taste sensation through the alkaliphile chloride channel in Drosophila DOI
Ting‐Wei Mi, J. Mack,

Wyatt Koolmees

et al.

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(3), P. 466 - 480

Published: March 20, 2023

Language: Английский

Citations

16
Tastant-receptor interactions: insights from the fruit fly DOI

Christian Arntsen,

Jacqueline Guillemin,

Kayla Audette

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: April 11, 2024

Across species, taste provides important chemical information about potential food sources and the surrounding environment. As details chemicals receptors responsible for gustation are discovered, a complex view of system is emerging with significant contributions from research using fruit fly, Drosophila melanogaster , as model organism. In this brief review, we summarize recent advances in their relevance to more broadly. Our goal highlight molecular mechanisms underlying first step gustatory circuits: ligand-receptor interactions primary cells. After an introduction how it encodes canonical modalities sweet, bitter, salty, describe insights into nature carboxylic acid amino detection context sour umami taste, respectively. analysis extends non-canonical including metals, fatty acids, bacterial components, highlights unexpected signaling pathways that have recently been identified Comparing intricate cellular underpinnings ligands detected vivo flies reveals both specific promiscuous receptor selectivity encoding. Throughout compare contextualize these findings mammalian not only emphasize conservation chemosensory systems, but demonstrate power organism elucidating neurobiology feeding.

Language: Английский

Citations

5
Mechanisms of Carboxylic Acid Attraction in Drosophila melanogaster DOI Open Access
Bhanu Shrestha, Youngseok Lee

Molecules and Cells, Journal Year: 2021, Volume and Issue: 44(12), P. 900 - 910

Published: Oct. 29, 2021

Sour is one of the fundamental taste modalities that enable perception in animals. Chemoreceptors embedded organs are pivotal to discriminate between different chemicals ensure survival. Animals generally prefer slightly acidic food and avoid highly alternatives. We recently proposed all acids aversive at high concentrations, a response mediated by low pH as well specific anions

Language: Английский

Citations

30
Zinc activation of OTOP proton channels identifies structural elements of the gating apparatus DOI Creative Commons
Bochuan Teng, Joshua P. Kaplan, Ziyu Liang

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: April 11, 2023

Otopetrin proteins (OTOPs) form proton-selective ion channels that are expressed in diverse cell types where they mediate detection of acids or regulation pH. In vertebrates there three family members: OTOP1 is required for formation otoconia the vestibular system and it forms receptor sour taste, while functions OTOP2 OTOP3 not yet known. Importantly, gating mechanisms any OTOP well understood. Here, we show zinc (Zn 2+) , as other transition metals including copper (Cu 2+ ), potently activates murine (mOTOP3). Zn pre-exposure increases magnitude mOTOP3 currents to a subsequent acid stimulus by much 10-fold. contrast, mOTOP2 insensitive activation . Swapping extracellular tm 11–12 linker between was sufficient eliminate confer on mOTOP2. Mutation alanine H531 E535 within H234 E238 5–6 reduced eliminated indicating these residues likely contribute activating site. Kinetic modeling data consistent with stabilizing opn2+en state channel, competing H + channels. These results establish linkers part apparatus target drug discovery. an essential micronutrient its will undoubtedly have important physiological sequelae.

Language: Английский

Citations

10
Harnessing Insect Chemosensory and Mechanosensory Receptors Involved in Feeding for Precision Pest Management DOI Creative Commons
Ting‐Wei Mi,

Cheng‐Wang Sheng,

C. Lee

et al.

Life, Journal Year: 2025, Volume and Issue: 15(1), P. 110 - 110

Published: Jan. 16, 2025

Chemosensation and mechanosensation are vital to insects’ survival behavior, shaping critical physiological processes such as feeding, metabolism, mating, reproduction. During insects rely on diverse chemosensory mechanosensory receptors distinguish between nutritious harmful substances, enabling them select suitable food sources while avoiding toxins. These distributed across various body parts, allowing detect environmental cues about quality adjust their behaviors accordingly. A deeper understanding of insect sensory physiology, especially during not only enhances our knowledge biology but also offers significant opportunities for practical applications. This review highlights recent advancements in research feeding-related receptors, covering a wide range species, from the model organism Drosophila melanogaster agricultural human pests. Additionally, this examines potential targeting precision pest control. Disrupting feeding reproduction emerges promising strategy management. By interfering with these essential behaviors, we can effectively control populations minimizing impacts promoting ecological balance.

Language: Английский

Citations

0
New dimensions in the molecular genetics of insect chemoreception DOI
Gaëlle J.S. Talross, John R. Carlson

Trends in Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
An inhibitory mechanism for suppressing high salt intake in Drosophila DOI
Manali Dey, Anindya Ganguly, Anupama Dahanukar

et al.

Chemical Senses, Journal Year: 2023, Volume and Issue: 48

Published: Jan. 1, 2023

Abstract High concentrations of dietary salt are harmful to health. Like most animals, Drosophila melanogaster attracted foods that have low salt, but show strong taste avoidance high foods. Salt in known on multiple classes neurons, activating Gr64f sweet-sensing neurons drive food acceptance and 2 others (Gr66a bitter Ppk23 salt) rejection. Here we find NaCl elicits a bimodal dose-dependent response which activity with depressed salt. also inhibits the sugar this action is independent neuron’s Consistent electrophysiological analysis, feeding suppression presence correlates inhibition neuron activity, remains if genetically silenced. Other salts such as Na2SO4, KCl, MgSO4, CaCl2, FeCl3 act behavior same way. A comparison effects various suggests dictated by cationic moiety rather than anionic component Notably, salt-dependent not observed Gr66a neurons—response canonical tastant, denatonium, altered Overall, study characterizes mechanism appetitive can deter ingestion potentially salts.

Language: Английский

Citations

9
The power of Drosophila genetics in studying insect toxicology and chemical ecology DOI Creative Commons
Jia Huang, Youngseok Lee

Crop Health, Journal Year: 2023, Volume and Issue: 1(1)

Published: Nov. 27, 2023

Language: Английский

Citations

9
Gating elements for carvacrol activation of the OTOP1 proton channel DOI Creative Commons
Jingmei Hu,

Yalan Han,

Anna Luo

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Sept. 9, 2024

Language: Английский

Citations

2
Pharyngeal neuronal mechanisms governing sour taste perception in Drosophila melanogaster DOI Creative Commons

Bhanu Shrestha,

Jiun Sang, Suman Rimal

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Oct. 2, 2024

Sour taste, which is elicited by low pH, may serve to help animals distinguish appetitive from potentially harmful food sources. In all species studied date, the attractiveness of oral acids contingent on concentration. Many carboxylic are attractive at ecologically relevant concentrations but become aversive beyond some maximal Recent work found that Drosophila ionotropic receptors IR25a and IR76b expressed sweet-responsive gustatory receptor neurons (GRNs) in labellum, a peripheral organ, mediate feeding behaviors toward dilute acids. Here, we disclose existence pharyngeal sensors melanogaster detect ingested also involved responses These rely IR51b, IR94a, IR94h, together with IR76b, drive We then demonstrate optogenetic activation either Ir94a + or Ir94h GRNs promotes an response, confirming their contributions behavior. Our discovery internal sour taste opens up new avenues for investigating sensation tastants insects.

Language: Английский

Citations

2