A molecular mechanism for high salt taste in Drosophila DOI Creative Commons
Sasha A.T. McDowell, Molly Stanley, Michael D. Gordon

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Feb. 28, 2022

SUMMARY Dietary salt detection and consumption are crucial to maintaining fluid ionic homeostasis. To optimize intake, animals employ salt-dependent activation of multiple taste pathways. Generally, sodium activates attractive cells, but attraction is overridden at high concentrations by cation non-selective aversive cells. In flies, avoidance driven both ‘bitter’ neurons a class glutamatergic ‘high salt’ expressing pickpocket23 (ppk23). Although the cellular basis has been described, many molecular mechanisms remain elusive. Here, we show that ionotropic receptor 7c (IR7c) expressed in neurons, where it functions with co-receptors IR76b IR25a detect salt. Misexpression IR7c sweet which endogenously express IR25a, confers responsiveness non-sodium salts, indicating sufficient convert sodium-selective receptor. Furthermore, resultant transformation neuron tuning switches potassium chloride from an tastant. This research provides insight into monovalent divalent coding full repertoire IRs needed form functional

Language: Английский

Molecular and neuronal mechanisms for amino acid taste perception in the Drosophila labellum DOI Creative Commons
Binod Aryal, Subash Dhakal, Bhanu Shrestha

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(6), P. 1376 - 1386.e4

Published: Feb. 16, 2022

Language: Английский

Citations

51
A molecular mechanism for high salt taste in Drosophila DOI Creative Commons
Sasha A.T. McDowell, Molly Stanley, Michael D. Gordon

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(14), P. 3070 - 3081.e5

Published: June 29, 2022

Language: Английский

Citations

42
Molecular sensors in the taste system of Drosophila DOI
Bhanu Shrestha, Youngseok Lee

Genes & Genomics, Journal Year: 2023, Volume and Issue: 45(6), P. 693 - 707

Published: Feb. 24, 2023

Language: Английский

Citations

23
Molecular Basis of Hexanoic Acid Taste in Drosophila melanogaster DOI Open Access
Roshani Nhuchhen Pradhan, Bhanu Shrestha, Youngseok Lee

et al.

Molecules and Cells, Journal Year: 2023, Volume and Issue: 46(7), P. 451 - 460

Published: May 19, 2023

Animals generally prefer nutrients and avoid toxic harmful chemicals.Recent behavioral physiological studies have identified that sweet-sensing gustatory receptor neurons (GRNs) in Drosophila melanogaster mediate appetitive behaviors toward fatty acids.Sweet-sensing GRN activation requires the function of ionotropic receptors IR25a, IR56d, IR76b, as well GR64e.However, we reveal hexanoic acid (HA) is rather than nutritious to D. melanogaster.HA one major components fruit Morinda citrifolia (noni).Thus, analyzed responses noni acids, HA, via electrophysiology proboscis extension response (PER) assay.Electrophysiological tests show this reminiscent arginine-mediated neuronal responses.Here, determined a low concentration HA induced attraction, which was mediated by GRNs, high aversion, bitter-sensing GRNs.We also demonstrated elicits attraction mainly GR64d IR56d expressed but activates three (GR32a, GR33a, GR66a) GRNs.The mechanism sensing biphasic dose dependent manner.Furthermore, inhibit sugar-mediated like other bitter compounds.Taken together, discovered binary HA-sensing may be evolutionarily meaningful foraging niche insects.

Language: Английский

Citations

20
A single pair of pharyngeal neurons functions as a commander to reject high salt in Drosophila melanogaster DOI Creative Commons
Jiun Sang, Subash Dhakal, Bhanu Shrestha

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 27, 2023

Salt (NaCl), is an essential nutrient for survival, while excessive salt can be detrimental. In the fruit fly, Drosophila melanogaster , internal taste organs in pharynx are critical gatekeepers impacting decision to accept or reject a food. Currently, our understanding of mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high rudimentary. Here, we found that member ionotropic family, Ir60b expressed exclusively pair GRNs activated by salt. Using two-way choice assay (DrosoX) measure ingestion volume, demonstrate IR60b and two co-receptors IR25a IR76b required prevent consumption. Mutants lacking external but retaining exhibit much higher avoidance than flies with all missing three IRs. Our findings highlight vital role IRs GRN control

Language: Английский

Citations

17
Avoiding alkaline taste through ionotropic receptors DOI Creative Commons
Prakash Pandey,

Bhanu Shrestha,

Youngseok Lee

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 110087 - 110087

Published: May 23, 2024

Taste organs contain distinct gustatory receptors that help organisms differentiate between nourishing and potentially harmful foods. The detection of high pH levels plays a crucial role in food selection, but the specific responsible for perceiving elevated foods have remained unknown. By using

Language: Английский

Citations

7
Protocol for binary food choice assays using Drosophila melanogaster DOI Creative Commons
Binod Aryal, Subash Dhakal, Bhanu Shrestha

et al.

STAR Protocols, Journal Year: 2022, Volume and Issue: 3(2), P. 101410 - 101410

Published: May 18, 2022

Food preference is a fundamental behavior for animals to choose nutritious foods while rejecting containing toxins. Here, we describe binary food choice assays using Drosophila melanogaster, which are straightforward approaches the characterization of two-way tastants. We detail preparation flies and dye-containing food, followed by binary-choice feeding determination index (PI). This protocol simple, sensitive, reproducible in qualitatively detecting attractive or aversive characteristics toward any For complete details on use execution this protocol, please refer Aryal et al. (2022).

Language: Английский

Citations

25
The taste of vitamin C in Drosophila DOI
Bhanu Shrestha, Binod Aryal, Youngseok Lee

et al.

EMBO Reports, Journal Year: 2023, Volume and Issue: 24(6)

Published: April 28, 2023

Language: Английский

Citations

16
Taste Detection of Flonicamid in Drosophila melanogaster DOI
Muhammad Atif, Youngseok Lee

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104302 - 104302

Published: March 1, 2025

Language: Английский

Citations

0
Harnessing Insect Chemosensory and Mechanosensory Receptors Involved in Feeding for Precision Pest Management DOI Creative Commons
Ting‐Wei Mi,

Cheng‐Wang Sheng,

C. Lee

et al.

Life, Journal Year: 2025, Volume and Issue: 15(1), P. 110 - 110

Published: Jan. 16, 2025

Chemosensation and mechanosensation are vital to insects’ survival behavior, shaping critical physiological processes such as feeding, metabolism, mating, reproduction. During insects rely on diverse chemosensory mechanosensory receptors distinguish between nutritious harmful substances, enabling them select suitable food sources while avoiding toxins. These distributed across various body parts, allowing detect environmental cues about quality adjust their behaviors accordingly. A deeper understanding of insect sensory physiology, especially during not only enhances our knowledge biology but also offers significant opportunities for practical applications. This review highlights recent advancements in research feeding-related receptors, covering a wide range species, from the model organism Drosophila melanogaster agricultural human pests. Additionally, this examines potential targeting precision pest control. Disrupting feeding reproduction emerges promising strategy management. By interfering with these essential behaviors, we can effectively control populations minimizing impacts promoting ecological balance.

Language: Английский

Citations

0