An RXLR effector disrupts vesicle trafficking at ER-Golgi interface for Phytophthora capsici pathogenicity DOI Open Access
Jihyun Kim, Jesse Kaleku, Haeun Kim

et al.

Molecules and Cells, Journal Year: 2024, Volume and Issue: unknown, P. 100158 - 100158

Published: Nov. 1, 2024

Phytophthora species, an oomycetes plant pathogen, secrete effectors into cell throughout their life cycle for manipulating host immunity to achieve successful colonization. However, the molecular mechanisms underlying effector-triggered necrotic death remain elusive. In this study, we identified RXLR effector (Pc12) from capsici which contributes virulence and induces necrosis by triggering a distinct endoplasmic reticulum (ER) stress response through its interaction with Rab13-2. The induced Pc12 did not exhibit conventional (ETI)-mediated hypersensitive death, including involvement of NLR downstream signaling components transcriptional reprogramming defense-related genes. Instead, it alters localization ER-resident proteins confines secretory within ER. directly interacts Rab13-2, is primarily localized ER Golgi apparatus, resulting in diminished Rab13-2 signal on apparatus. Furthermore, exhibits increased affinity interactor, Rab escort protein 1 (REP1), presence Pc12. Structural predictions revealed that specific residue crucial binding C-terminus Substitution reduced impaired P. infection, while maintaining REP1 prenylated acceptor (PRA1). These findings provide insight how pathogen form facilitate colonization disrupting recycling involved vesicle trafficking at ER-Golgi interface.

Language: Английский

Pathogen perception and signaling in plant immunity DOI Creative Commons
Peter N. Dodds, Jian Chen, Megan A. Outram

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(5), P. 1465 - 1481

Published: Jan. 23, 2024

Abstract Plant diseases are a constant and serious threat to agriculture ecological biodiversity. Plants possess sophisticated innate immunity system capable of detecting responding pathogen infection prevent disease. Our understanding this has grown enormously over the past century. Early genetic descriptions plant disease resistance virulence were embodied in gene-for-gene hypothesis, while physiological studies identified pathogen-derived elicitors that could trigger defense responses cells tissues. Molecular these phenomena have now coalesced into an integrated model involving cell surface intracellular detection specific molecules proteins culminating induction various cellular responses. Extracellular receptors engage distinct signaling processes but converge on many similar outputs with substantial evidence for integration pathways interdependent networks controlling outcomes. Many molecular details recognition known, providing opportunities bioengineering enhance protection from Here we provide overview current main principles immunity, emphasis key scientific milestones leading insights.

Language: Английский

Citations

32
Uptake of oomycete RXLR effectors into host cells by clathrin-mediated endocytosis DOI Creative Commons
Haixia Wang, Shumei Wang, Wei Wang

et al.

The Plant Cell, Journal Year: 2023, Volume and Issue: 35(7), P. 2504 - 2526

Published: March 13, 2023

Abstract Filamentous (oomycete and fungal) plant pathogens deliver cytoplasmic effector proteins into host cells to facilitate disease. How RXLR effectors from the potato late blight pathogen Phytophthora infestans enter is unknown. One possible route involves clathrin-mediated endocytosis (CME). Transient silencing of NbCHC, encoding clathrin heavy chain, or endosome marker gene NbAra6 a Rab GTPase in model Nicotiana benthamiana, attenuated P. infection reduced translocation fusions transgenic strains cells. By contrast, PP1c isoforms, susceptibility factors not required for endocytosis, but did attenuate uptake. Endosome enrichment by ultracentrifugation sucrose gradient fractionation revealed co-localization Pi04314-RFP with clathrin-coated vesicles. Immunopurification clathrin- NbAra6-associated vesicles during showed that AvrBlb1-RFP, apoplastic PiSCR74-RFP, were co-immunoprecipitated secreting these effectors. Tandem mass spectrometry analyses NbAra6-GFP associated endocytic alongside multiple effectors, including PiSCR74, which do Our data show uptake occurs via CME.

Language: Английский

Citations

38
Symbiont-host interactome mapping reveals effector-targeted modulation of hormone networks and activation of growth promotion DOI Creative Commons
Rory Osborne, Laura Rehneke, Silke Lehmann

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 10, 2023

Abstract Plants have benefited from interactions with symbionts for coping challenging environments since the colonisation of land. The mechanisms symbiont-mediated beneficial effects and similarities differences to pathogen strategies are mostly unknown. Here, we use 106 (effector-) proteins, secreted by symbiont Serendipita indica ( Si ) modulate host physiology, map Arabidopsis thaliana proteins. Using integrative network analysis, show significant convergence on target-proteins shared pathogens exclusive targeting proteins in phytohormone signalling network. Functional planta screening phenotyping effectors interacting reveals previously unknown hormone functions direct activities mediated Arabidopsis. Thus, target a molecular microbe-host interface. At same time specifically plant constitute powerful resource elucidating function boosting productivity.

Language: Английский

Citations

19
Recent developments in plant-downy mildew interactions DOI Creative Commons
Mahmut Tör,

Tom Wood,

Anne Webb

et al.

Seminars in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 148-149, P. 42 - 50

Published: Jan. 18, 2023

Downy mildews are obligate oomycete pathogens that attack a wide range of plants and can cause significant economic impacts on commercial crops ornamental plants. Traditionally, downy mildew disease control relied an integrated strategies, incorporate cultural practices, deployment resistant cultivars, crop rotation, application contact systemic pesticides, biopesticides. Recent advances in genomics provided data significantly advanced understanding evolution, taxonomy classification. In addition, also revealed these oomycetes have reduced numbers virulence factor genes comparison to hemibiotrophic necrotrophic oomycetes. However, do deploy arrays proteins, including so-called RXLR proteins promote or recognized as avirulence factors. Pathogenomics being applied population studies determine the genetic diversity within populations manage by selection appropriate varieties management strategies. Genome editing technologies been used manipulate host susceptibility different grapevine sweet basil thereby provide new soucres resistance against mildews. Previously, it has proved difficult transform because their lifestyle. recent exploitation RNA interference machinery through Host-Induced Gene Silencing (HIGS) Spray-Induced (SIGS) indicate functional is now possible. Altogether, breakthrough attendant fundamental will advance our ability mitigate diseases.

Language: Английский

Citations

17
Plant genes related to Phytophthora pathogens resistance DOI Creative Commons
Qi Li, Hai Zhu, Gan Ai

et al.

Phytopathology Research, Journal Year: 2024, Volume and Issue: 6(1)

Published: Feb. 29, 2024

Abstract Plants have evolved a multilayered and sophisticated immune system to establish effective resistance variety of pathogens. Phytophthora species are among the most notorious plant pathogens, causing destructive diseases on agricultural crops. Understanding is crucial for protecting crops from diseases. Here, we summarize recent work genes involved in against including cell surface pattern recognition receptors, cytoplasmic nucleotide-binding leucine-rich repeat regulator genes, non-host small RNA, long non-coding RNA also discussed this review. Although molecular mechanisms only proportion them been clarified, emergence new defense will offer exciting opportunities utilization these disease breeding as well generation disease-resistant crop germplasms.

Language: Английский

Citations

6
Chloroplast elongation factors break the growth–immunity trade-off by simultaneously promoting yield and defence DOI

Yetong Qi,

Jiahui Wu,

Zhu Yang

et al.

Nature Plants, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 19, 2024

Language: Английский

Citations

5
A tell tail sign: a conserved C-terminal tail-anchor domain targets a subset of pathogen effectors to the plant endoplasmic reticulum DOI Creative Commons
Emily Breeze,

Victoria Vale,

Hazel McLellan

et al.

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 74(10), P. 3188 - 3202

Published: March 1, 2023

Abstract The endoplasmic reticulum (ER) is the entry point to secretory pathway and, as such, critical for adaptive responses biotic stress, when demand de novo synthesis of immunity-related proteins and signalling components increases significantly. Successful phytopathogens have evolved an arsenal small effector which collectively reconfigure multiple host pathways promote virulence; a small, but important, subset are targeted endomembrane system including ER. We identified validated conserved C-terminal tail-anchor motif in set pathogen effectors known localize ER from oomycetes Hyaloperonospora arabidopsidis Plasmopara halstedii (downy mildew Arabidopsis sunflower, respectively) used this protein topology develop bioinformatic pipeline identify putative ER-localized within effectorome related oomycete, Phytophthora infestans, causal agent potato late blight. Many P. infestans converged on NAC transcription factors, indicating that family target pathogens.

Language: Английский

Citations

11
Phytophthora RxLR effector Pi18609 suppresses host immunity by promoting turnover of a positive immune regulator StBBX27 DOI Open Access

Qingguo Sun,

M. Fan,

Huishan Qiu

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(5)

Published: March 1, 2025

Oomycete pathogens deliver a number of RxLR effector proteins into host plant cells to manipulate immunity by targeting diverse proteins. Here, we reveal that Phytophthora infestans Pi18609 targets potato B-box transcription factor (BBX), StBBX27, which was identified positively regulate late blight resistance. Silencing the ortholog NbBBX27 in Nicotiana benthamiana or RNA interfering StBBX27 increases colonization P. infestans, whereas transient expression N. stable overexpression enhances Overexpression activates immune responses including upregulation defense-related genes such as StWRKY8, StOSML13, StCHTB4, and StSERK2, burst reactive oxygen species (ROS). In addition, directly binds G-box WRKY8 SERK2 promoters their expression, could be suppressed Pi18609. Furthermore, revealed ubiquitin E3 ligase COP1 promotes turnover proteasome-mediated system. Moreover, degradation mediated COP1. Collectively, this study reveals regulates is promote its turnover. This research extends our knowledge on BBXs function mechanisms behind effectors suppression immunity.

Language: Английский

Citations

0
FIRE Mimics a 14-3-3–Binding Motif to Promote Phytophthora palmivora Infection DOI Creative Commons
Édouard Evangelisti,

Alex Guyon,

Liron Shenhav

et al.

Molecular Plant-Microbe Interactions, Journal Year: 2023, Volume and Issue: 36(6), P. 315 - 322

Published: Feb. 3, 2023

The oomycete Phytophthora palmivora infects a wide range of tropical crops worldwide. Like other filamentous plant pathogens, it secretes effectors to colonize tissues. Here, we characterize FIRE, an RXLR effector that contains canonical mode I 14-3-3 phospho-sensor–binding motif is conserved in several species. FIRE phosphorylated planta and interacts with multiple proteins. Binding sensitive the R18 inhibitor. promotes susceptibility co-localizes its target around haustoria. This work uncovers new type mechanism. It demonstrates substrate mimicry for proteins cross-kingdom strategy used by both prokaryotic eukaryotic pathogens suppress host immunity. [Formula: see text] Copyright © 2023 Author(s). open access article distributed under CC BY 4.0 International license .

Language: Английский

Citations

10
Tuning the Wavelength: Manipulation of Light Signaling to Control Plant Defense DOI Open Access
Susan Breen, Hazel McLellan, Paul R. J. Birch

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3803 - 3803

Published: Feb. 14, 2023

The growth-defense trade-off in plants is a phenomenon whereby must balance the allocation of their resources between developmental growth and defense against attack by pests pathogens. Consequently, there are series points where signaling can negatively regulate defenses inhibit growth. Light perception various photoreceptors has major role control thus many it influence defense. Plant pathogens secrete effector proteins to manipulate hosts. Evidence emerging that some these effectors target light pathways. Several from different kingdoms life have converged on key chloroplast processes take advantage regulatory crosstalk. Moreover, plant also perceive react complex ways own growth, development, virulence. Recent work shown varying wavelengths may provide novel way controlling or preventing disease outbreaks plants.

Language: Английский

Citations

10