Control of intracellular pH and bicarbonate by CO2 diffusion into human sperm

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 5, 2023

The reaction of CO2 with H2O to form bicarbonate (HCO3-) and H+ controls sperm motility fertilization via HCO3--stimulated cAMP synthesis. A complex network signaling proteins participates in this reaction. Here, we identify key players that regulate intracellular pH (pHi) HCO3- human by quantitative mass spectrometry (MS) kinetic patch-clamp fluorometry. resting pHi is set amiloride-sensitive Na+/H+ exchange. sperm-specific putative exchanger SLC9C1, unlike its sea urchin homologue, not gated voltage or cAMP. Transporters channels implied transport are detected, may be present at copy numbers < 10 molecules/sperm cell. Instead, produced diffusion into cells readjustment the CO2/HCO3-/H+ equilibrium. proton channel Hv1 serve as a unidirectional valve blunts acidification ensuing from This work provides new framework for study male infertility.

Language: Английский

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Time series transcriptome analysis implicates the circadian clock in the Drosophila melanogaster female’s response to sex peptide

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(5)

Published: Jan. 27, 2023

Sex peptide (SP), a seminal fluid protein of

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Functional Diversity and Evolution of the Drosophila Sperm Proteome

Molecular & Cellular Proteomics, Journal Year: 2022, Volume and Issue: 21(10), P. 100281 - 100281

Published: Aug. 17, 2022

Spermatozoa are central to fertilization and the evolutionary fitness of sexually reproducing organisms. As such, a deeper understanding sperm proteomes (and associated reproductive tissues) has proven critical advancement fields sexual selection biology. Due their extraordinary complexity, proteome depth-of-coverage is dependent on advancements in technology related bioinformatics, both which have made significant decade since last Drosophila was published. Here, we provide an updated version melanogaster (DmSP3) using improved separation detection methods genome annotation. Combined with previous versions proteome, DmSP3 contains total 3176 proteins, first label-free quantitation for 2125 proteins. The top 20 most abundant proteins included structural elements α- β-tubulins leucyl-aminopeptidases. Both gene content protein abundance were significantly reduced X chromosome, consistent prior genomic studies chromosome evolution. We identified 9 16 Y-linked including known testis-specific male fertility factors. also almost one-half ribosomal DmSP3. role this subset unknown. Surprisingly, our expanded 122 seminal fluid (Sfps), originally accessory glands. show that fraction 'sperm-associated Sfps' recalcitrant concentrated salt detergent treatments, suggesting subclass Sfps expressed testes may additional functions sperm, per se. Overall, results add growing landscape biology particular provides quantitative evidence at level findings supporting meiotic sex-chromosome inactivation model male-specific

Language: Английский

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Synthesis and Scope of the Role of Postmating Prezygotic Isolation in Speciation

Cold Spring Harbor Perspectives in Biology, Journal Year: 2023, Volume and Issue: 16(10), P. a041429 - a041429

Published: Dec. 27, 2023

How barriers to gene flow arise and are maintained key questions in evolutionary biology. Speciation research has mainly focused on that occur either before mating or after zygote formation. In comparison, postmating prezygotic (PMPZ) isolation-a barrier acts gamete release but formation-is less frequently investigated may hold a unique role generating biodiversity. Here we discuss the distinctive features of PMPZ isolation, including primary drivers molecular mechanisms underpinning isolation. We then present first comprehensive survey isolation research, revealing it is widespread form across eukaryotes. The also exposes obstacles studying part attributable challenges involved directly measuring uncovering its causal mechanisms. Finally, identify outstanding knowledge gaps provide recommendations for improving future This will allow us better understand nature this often-neglected reproductive contribution speciation.

Language: Английский

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Deciphering dynamic interactions between spermatozoa and the ovarian microenvironment through integrated multi-omics approaches in viviparous Sebastes schlegelii

Development, Journal Year: 2024, Volume and Issue: 151(20)

Published: April 4, 2024

ABSTRACT The ovarian microenvironment plays a crucial role in ensuring the reproductive success of viviparous teleosts. However, molecular mechanism underlying interaction between spermatozoa and has remained elusive. This study aimed to contribute better understanding this process black rockfish (Sebastes schlegelii) using integrated multi-omics approaches. results demonstrated significant upregulation complement-related proteins pattern recognition receptors, along with remodeling glycans on surface at early spermatozoa-storage stage (1 month after mating). As were stored over time, complement progressively repressed by tryptophan hippurate, indicating remarkable adaptation microenvironment. Before fertilization, notable cellular junction was observed. revealed that bind ZPB2a protein through GSTM3 promotes survival movement GSTM3-dependent manner. These findings shed light key influences dynamics female tract, providing valuable insights into networks regulating species internal fertilization.

Language: Английский

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Post-Mating Responses in Insects Induced by Seminal Fluid Proteins and Octopamine

Biology, Journal Year: 2023, Volume and Issue: 12(10), P. 1283 - 1283

Published: Sept. 26, 2023

Following insect mating, females often exhibit a series of physiological, behavioral, and gene expression changes. These post-mating responses (PMRs) are induced by seminal fluid components other than sperm, which not only form network proteins to assist sperm localization, supplement female-specific protein requirements, facilitate the formation specialized functional structures, but also activate neuronal signaling pathways in insects. This review primarily discusses roles (SFPs) octopamine (OA) various PMRs It explores regulatory mechanisms mediation conditions they trigger PMRs, along with differences induce. Insect involve transition from signaling, ultimately manifested through neural regulation expression. The intricate formed as result significantly influences female behavior organ function, contributing both successful reproduction outcomes sexual conflict.

Language: Английский

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Characterization of reproductive proteins in the Mexican fruit fly points towards the evolution of novel functions

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2022, Volume and Issue: 289(1977)

Published: June 29, 2022

Seminal fluid proteins (Sfps) modify female phenotypes and have wide-ranging evolutionary implications on fitness in many insects. However, the Mexican fruit fly, Anastrepha ludens , a highly destructive agricultural pest, functions of Sfps are still largely unknown. To gain insights into regulated by Sfps, we used nano-liquid chromatography mass spectrometry to conduct proteomic analysis soluble from reproductive organs A. . The predicted be transferred males females during copulation were 100 accessory glands, 69 testes 20 ejaculatory bulb, resulting 141 unique after accounting for redundancies multiple tissues. These included orthologues Drosophila melanogaster involved mainly oogenesis, spermatogenesis, immune response, lifespan fecundity. In particular, found one protein associated with olfactory response repellent stimuli (Scribble), two related memory formation (aPKC Shibire). Together, these results raise possibility that could play role regulating responses indicative novel this important pest.

Language: Английский

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Females translate male mRNA transferred during mating

iScience, Journal Year: 2024, Volume and Issue: 27(8), P. 110442 - 110442

Published: July 3, 2024

Although RNA is found in the seminal fluid of diverse organisms, it unknown whether functional within females. We developed a proteomic method (VESPA, Variant Enabled SILAC Proteomic Analysis) to test hypothesis that Drosophila male translated by 67 male-derived, female-translated proteins (mdFTPs) female lower reproductive tracts, many with predicted functions relevant reproduction. Knockout experiments indicate mdFTPs play roles postmating interactions, affecting fertilization success, and formation/persistence insemination reaction mass, trait hypothesized be involved sexual conflict. These findings advance our understanding reproduction revealing mechanism molecular interactions between sexes strengthens extends influences on previously unrecognized ways. Given species carry fluid, this discovery has broad significance for mechanisms cooperation conflict during

Language: Английский

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Plasticity in moth mating behavior and ejaculate proteomic diversity induced by male competition

Journal of Insect Physiology, Journal Year: 2024, Volume and Issue: 156, P. 104673 - 104673

Published: July 1, 2024

Language: Английский

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An Orphan Gene Enhances Male Reproductive Success in Plutella xylostella

Molecular Biology and Evolution, Journal Year: 2024, Volume and Issue: 41(7)

Published: July 1, 2024

Abstract Plutella xylostella exhibits exceptional reproduction ability, yet the genetic basis underlying high reproductive capacity remains unknown. Here, we demonstrate that an orphan gene, lushu, which encodes a sperm protein, plays crucial role in male success. Lushu is located on Z chromosome and prevalent across different P. populations worldwide. We subsequently generated lushu mutants using transgenic CRISPR/Cas9 system. Knockout of results reduced mating efficiency accelerated death adult males. Furthermore, our findings highlight deficiency transfer sperms from males to females, potentially resulting hindered competition. Additionally, knockout disrupted gene expression energy-related pathways elevated insulin levels Our reveal performance has evolved through birth newly evolved, lineage-specific with enormous potentiality fecundity These insights hold valuable implications for identifying target control, particularly relation species-specific traits are pivotal determining fecundity.

Language: Английский

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