Aptamers 101: aptamer discovery andin vitroapplications in biosensors and separations

Chemical Science, Journal Year: 2023, Volume and Issue: 14(19), P. 4961 - 4978

Published: Jan. 1, 2023

A primer that covers the discovery and validation process for novel aptamers highlights recent applications of in biosensing cell separations.

Language: Английский

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Applications of Graphene in Five Senses, Nervous System, and Artificial Muscles

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(2), P. 482 - 514

Published: Jan. 19, 2023

Graphene remains of great interest in biomedical applications because biocompatibility. Diseases relating to human senses interfere with life satisfaction and happiness. Therefore, the restoration by artificial organs or sensory devices may bring a bright future recovery patients. In this review, we update most recent progress graphene based sensors for mimicking such as retina image sensors, eardrums, gas chemical tactile sensors. The brain-like processors are discussed on conventional transistors well memristor related neuromorphic computing. brain–machine interface is introduced providing single pathway. Besides, muscles summarized means actuators order react physical world. Future opportunities remain elevating performances human-like their clinical applications.

Language: Английский

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Long-term monitoring of ultratrace nucleic acids using tetrahedral nanostructure-based NgAgo on wearable microneedles

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 2, 2024

Abstract Real-time and continuous monitoring of nucleic acid biomarkers with wearable devices holds potential for personal health management, especially in the context pandemic surveillance or intensive care unit disease. However, achieving high sensitivity long-term stability remains challenging. Here, we report a tetrahedral nanostructure-based Natronobacterium gregoryi Argonaute (NgAgo) stable ultratrace unamplified acids (cell-free DNAs RNAs) vivo sepsis on device. This integrated wireless consists flexible circuit board, microneedle biosensor, stretchable epidermis patch enrichment capability. We comprehensively investigate recognition mechanism by NgAgo/guide DNA signal transformation within Debye distance. In experiments demonstrate suitability real-time cell-free RNA 0.3 fM up to 14 days. These results provide strategy highly sensitive molecular on-body detection acid.

Language: Английский

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Graphene and its derivatives (GO, rGO and GQD): a comprehensive review of their role in combating COVID-19

Advances in Physics X, Journal Year: 2025, Volume and Issue: 10(1)

Published: Jan. 15, 2025

The COVID-19 pandemic has ignited a search for innovative solutions to tackle viral transmission and ensure early detection. Graphene its derivatives have emerged as promising materials in this fight, showcasing impressive potential across diverse range of applications. From frontline defenses like antiviral PPE with enhanced filtration breathability microscopic detective work via highly sensitive FET biosensors, graphene offers multifaceted attack on the SARS-CoV-2 virus. Its impact extends beyond diagnostics, empowering energy storage disinfection technologies while revolutionizing drug delivery air purification through virus-capturing filters. Antiviral coatings vigilant nano-sensors further bolster arsenal, offering comprehensive protection at surface levels. Moreover, graphene-based masks, their reduce pollution exposure, may even offer an additional layer against polluted areas. In summary, emerges versatile guardian COVID-19, poised revolutionize fight beyond. applications, coupled ongoing research, paint compelling picture future where one atom time, rewrites narrative defense.

Language: Английский

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Amplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samples

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(11)

Published: March 10, 2025

Rapid and accurate detection of DNA from disease-causing pathogens is essential for controlling the spread infections administering timely treatments. While traditional molecular diagnostics techniques like PCR are highly sensitive, they include nucleic acid amplification many need to be performed in centralized laboratories, limiting their utility point-of-care settings. Recent advances CRISPR-based (CRISPR-Dx) have demonstrated potential specific detection, but sensitivity often constrained by slow trans-cleavage activity Cas enzymes, necessitating preamplification target acids. In this study, we present a CRISPR-Cascade assay that overcomes these limitations integrating positive feedback loop enables amplification-free pathogenic at atto-molar levels achieves signal-to-noise ratio greater than 1.3 within just 10 min. The versatility through bloodstream infection pathogens, including Methicillin-Sensitive Staphylococcus aureus (MSSA), Methicillin-Resistant (MRSA), Escherichia coli , Hepatitis B Virus (HBV) spiked whole blood samples. Additionally, introduce multiplexing OR-function logic gate, further enhancing rapid clinical Our findings highlight ability provide sensitive paving way advanced applications beyond.

Language: Английский

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Wireless, battery-free, multifunctional integrated bioelectronics for respiratory pathogens monitoring and severity evaluation

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 20, 2023

The rapid diagnosis of respiratory virus infection through breath and blow remains challenging. Here we develop a wireless, battery-free, multifunctional pathogenic system (PIDS) for diagnosing SARS-CoV-2 symptom severity by within 110 s 350 s, respectively. accuracies reach to 100% 92% evaluating the 42 participants, PIDS realizes simultaneous gaseous sample collection, biomarker identification, abnormal physical signs recording machine learning analysis. We transform into other miniaturized wearable or portable electronic platforms that may widen diagnostic modes at home, outdoors public places. Collectively, demonstrate general-purpose technology rapidly blow, alleviating technical bottleneck saliva nasopharyngeal secretions. serve as complementary tool point-of-care techniques guide symptomatic treatment viral infections.

Language: Английский

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Graphene Transistors for In Vitro Detection of Health Biomarkers

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(31)

Published: May 10, 2023

Abstract Biomarkers are primary indicators for precise diagnosis and treatment. The early identification of health biomarkers has been sustained by the evolutionary success in sensor technologies. Among them, graphene field‐effect transistor (GFET) biosensors have exhibited major advantages such as an ultrashort response time, high sensitivity, easy operation, capability integration, label‐free detection. Owing to atomic thickness, restricts charge carrier flow merely at material surface responds foreign stimuli directly, leading effective signal acquisition transmission. Here, this review summarizes latest advances GFET a comprehensive manner that contains device design, working principle, functionalization, proof‐of‐concept applications. It provides survey with regard biomarker analysis single‐device level integrated prototypes include wearable sensors, biomimetic systems, healthcare electronics, diagnostic platforms. Moreover, there is discussion on long‐standing research efforts outlook future development systems from lab fab.

Language: Английский

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Aptamer-based assembly systems for SARS-CoV-2 detection and therapeutics

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(13), P. 6830 - 6859

Published: Jan. 1, 2024

This review presents the recent progress on aptamers that have been explored for SARS-CoV-2 detection and therapeutics, wherein construction principles characteristics of aptamer-based assembly systems are systematically summarized.

Language: Английский

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Non-invasive Monitoring of α-Synuclein in Saliva for Parkinson’s Disease Using Organic Electrolyte-Gated FET Aptasensor

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(8), P. 3116 - 3126

Published: July 28, 2023

Parkinson's disease (PD) currently affects more than 1 million people in the US alone, with nearly 8.5 suffering from worldwide, as per World Health Organization. However, there remains no fast, pain-free, and effective method of screening for ageing population, which also happens to be most susceptible this neurodegenerative disease. αSynuclein (αSyn) is a promising PD biomarker, demonstrating clear delineations between levels αSyn monomer extent aggregation saliva patients healthy controls. In work, we have demonstrated laboratory prototype soft fluidics integrated organic electrolyte-gated field-effect transistor (OEGFET) aptasensor platform capable quantifying saliva. The relies on recently reported synthetic aptamer selectively binds bio-recognition molecule within fluidic channel biosensor. produced sensor label-free, reusable, good selectivity only target its form. novelty these devices fully isolated semiconductor, extends shelf life, novel microfluidic channels, simplify loading testing. OEGFET has limit detection 10 fg/L spiked supernatant solutions, linear range 100 μg/L. covers physiological patients. Our biosensors demonstrate desirably low detection, an extended range, microchannels sample handling, making them non-invasive point-of-care testing PD.

Language: Английский

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Role of Outer Surface Probes on Bullet-Shaped Asymmetric Solid-State Nanochannels for Lysozyme Protein Sensing

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(6), P. 2445 - 2454

Published: Jan. 31, 2024

Artificial solid-state nanochannels featuring precise partitions present a highly promising platform for biomarker detection. While the significance of probes on outer surface (POS) has been relatively overlooked in past, our research highlights their crucial role biosensing. Furthermore, contribution POS bullet-shaped asymmetric not extensively explored until now. Here, we fabricated series nanochannels, each distinct structure characterized by different tip- and base-pore diameters. These were further modified with explicit distributions at inner wall (PIW), (POS), combination (POS + PIW) lysozyme sensing. The impact diameters, structural asymmetry, charge density sensing efficacy PIW was thoroughly examined through experimental investigations numerical simulations. demonstrates great individual performance within broad concentration range, spanning from 10 nM to 1 mM. it improves sensitivity when combined PIW, particularly smaller diameter, resulting 2-fold increase compared nM. findings are substantiated simulations that closely align parameters. contributions notably amplified presence base pores higher degree asymmetry nanochannels. elucidate mechanism underlying open up new avenues manipulating enhancing efficiency.

Language: Английский

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