SARS‐CoV‐2 N Gene‐Targeted Anodic Stripping Voltammetry Sensor Using a Novel CoS‐NGQD/Pt@Pd Platform and Au‐DNA‐CdTe QD Probe DOI
Sathish Panneer Selvam, Le Minh Tu Phan, Sungbo Cho

et al.

Advanced Materials Technologies, Journal Year: 2023, Volume and Issue: 8(10)

Published: Jan. 31, 2023

Abstract Rapid screening of individuals infected with severe acute respiratory syndrome‐coronavirus‐2 (SARS‐CoV‐2) is necessary to contain contagion in a large population. Nucleic acid‐based gold standard assays are time‐consuming, and nucleic acid amplification mandatory expensive, impeding the containment coronavirus disease 2019 (COVID‐19) outbreak. To overcome aforementioned disadvantages, this study deals specially designed (Au)‐deoxyribonucleic (DNA)‐cadmium telluride (CdTe) quantum dot (QD) probe target two sections nucleocapsid (N) gene SARS‐CoV‐2 ribonucleic (RNA) three variants (B.1.1.529, B.1.617.2, B.1.351). A duplex‐specific nuclease (DSN)‐assisted highly selective release signaling probes enable higher specificity, an Au‐supported DNA incorporated carry many CdTe QD probes. After dissolution, generated Cd 2+ ions quantified at novel cobalt sulfide (CoS)‐nitrogen‐doped graphene (NGQD)/platinum (Pt)@palladium (Pd) electrode extraordinary sensitivity through square wave anodic stripping voltammetry (SWASV). The developed sensor exhibits wide range detection (10 10 8 copies µL −1 ) lower limit (0.12 ), without any amplification. selectivity tested against MERS HCoV‐NL63, real‐time performed on heat‐inactivated viral samples, which show excellent selectivity.

Language: Английский

Synthesis of Molecularly Imprinted Polymer Nanoparticles for SARS-CoV-2 Virus Detection Using Surface Plasmon Resonance DOI Creative Commons
Aabha Bajaj, Jakob Trimpert, Ibrahim Abdulhalim

et al.

Chemosensors, Journal Year: 2022, Volume and Issue: 10(11), P. 459 - 459

Published: Nov. 5, 2022

COVID-19 caused by a SARS-CoV-2 infection was first reported from Wuhan, China, and later recognized as pandemic on March 11, 2020, the World Health Organization (WHO). Gold standard nucleic acid molecular-based testing have largely satisfied requirements of early diagnosis management this infectious disease; however, these techniques are expensive not readily available for point-of-care (POC) applications. The 21st century has emphasized that medicine is in dire need advanced, rapid, cheap diagnostic tools. Herein, we report molecularly imprinted polymer nanoparticles (MIP-NPs/nanoMIPs) plastic antibodies specific detection employing surface plasmon resonance (SPR) sensor. High-affinity MIP-NPs directed against were manufactured using solid-phase imprinting method. then characterized dynamic light scattering (DLS) atomic force microscopy (AFM) prior to their incorporation into label-free portable SPR device. Detection studied within range 104–106 PFU mL−1. demonstrated good binding affinity (KD = 0.12 pM) selectivity toward SARS-CoV-2. AFM, cyclic voltammetry, square-wave voltammetry studies revealed successful stepwise preparation A cross-reactivity test confirmed specificity For time, study demonstrates potential molecular technology conjunction with miniaturized devices particles high-affinity specificity. Such sensors could help monitor manage risks related virus contamination infections also beyond current pandemic.

Language: Английский

Citations

23
A Single Multiomics Transistor for Electronic Detection of SARS‐Cov2 Variants Antigen and Viral RNA Without Amplification DOI Creative Commons
Deependra Kumar Ban, Reza Hajian, Alexander Winton

et al.

Advanced Materials Technologies, Journal Year: 2023, Volume and Issue: 8(11)

Published: April 4, 2023

Abstract The SARS‐CoV‐2 pandemic caused a public health crisis throughout the world and highlighted need for rapid sensitive testing as countermeasure. A specific biosensor platform is developed detection of antigen RNA SARS‐CoV‐2, its variant (B1.1.529). demonstrated combines unique protein catalyzed capture bioreceptors (PCCs) chimeric (RNA‐DNA) probe using LwaCas13a collateral cleavage activity atop graphene field effect transistors (gFETs). reported able to differentiate unprocessed 10 4 pfu m −1 samples from Influenza Rhinovirus. limit (LOD) calculated 3 in buffer PFU mL 10% saliva, while LOD ≈65 viral isolate without amplification. To provide high reliability detection, role internal external factors with respect gate voltage further analyzed by Principal Component Analysis (PCA). Based on PCA analysis, authors are classify pathogen positive or negative ( Y > 0: Positive pathogen, < Negative pathogen). can be quickly adapted multi‐omics multiplexed diagnosis continuously evolving biothreats global pandemics.

Language: Английский

Citations

14
Advances in nanobiosensors during the COVID-19 pandemic and future perspectives for the post-COVID era DOI Creative Commons
Young Jun Kim, Junhong Min

Nano Convergence, Journal Year: 2024, Volume and Issue: 11(1)

Published: Jan. 11, 2024

Abstract The unprecedented threat of the highly contagious virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes exponentially increased infections disease 2019 (COVID-19), highlights weak spots current diagnostic toolbox. In midst catastrophe, nanobiosensors offer a new opportunity as an alternative tool to fill gap among molecular tests, rapid antigen and serological tests. Nanobiosensors surpass potential tests because their enhanced sensitivity, thus enabling us see antigens stable easy-to-access targets. During first three years COVID-19 pandemic, substantial number studies have reported for detection SARS-CoV-2 antigens. articles on exceeds amount nanobiosensor research detecting previous infectious diseases, from influenza SARS-CoV MERS-CoV. This publishing pace also implies significance present pandemic. this review, 158 reporting are collected discuss challenges using criteria point-of-care (POC) diagnostics along with COVID-specific issues. These advances lessons during pandemic pave way preparing post-COVID era upcoming diseases. Graphical

Language: Английский

Citations

6
Interplay of graphene–DNA interactions: Unveiling sensing potential of graphene materials DOI
Yanjing Gao, Yichun Wang

Applied Physics Reviews, Journal Year: 2024, Volume and Issue: 11(1)

Published: Jan. 26, 2024

Graphene-based materials and DNA probes/nanostructures have emerged as building blocks for constructing powerful biosensors. possess exceptional properties, including two-dimensional atomically flat basal planes biomolecule binding. probes serve excellent selective probes, exhibiting specific recognition capabilities toward diverse target analytes. Meanwhile, nanostructures function placement scaffolds, enabling the precise organization of molecular species at nanoscale positioning complex biomolecular assays. The interplay graphene-based has fostered creation intricate hybrid with user-defined architectures. This advancement resulted in significant progress developing novel biosensors detecting DNA, RNA, small molecules, proteins, well sequencing. Consequently, a profound understanding interactions between is key to these biological devices. In this review, we systematically discussed current comprehension interaction materials, elucidated latest advancements probe–graphene-based Additionally, concisely summarized recent research endeavors involving deposition on explored imminent biosensing applications by seamlessly integrating materials. Finally, delineated primary challenges provided prospective insights into rapidly field. We envision that review will aid researchers gaining deeper insight mechanisms DNA–graphene-based biosensors, designing desired applications.

Language: Английский

Citations

6
Advances in field-effect biosensors towards point-of-use DOI Creative Commons
Sihan Chen, Rashid Bashir

Nanotechnology, Journal Year: 2023, Volume and Issue: 34(49), P. 492002 - 492002

Published: Aug. 25, 2023

The future of medical diagnostics calls for portable biosensors at the point care, aiming to improve healthcare by reducing costs, improving access, and increasing quality-what is called 'triple aim'. Developing point-of-care sensors that provide high sensitivity, detect multiple analytes, real time measurements can expand access all. Field-effect transistor (FET)-based have several advantages, including ultrahigh label-free amplification-free detection, reduced cost complexity, portability, large-scale multiplexing. They also be integrated into wearable or implantable devices continuous, real-time monitoring analytesin vivo, enabling early detection biomarkers disease diagnosis management. This review analyzes advances in parallelization, reusability FET biosensors, benchmarks limit state art, discusses challenges opportunities applications.

Language: Английский

Citations

12
In pursuit of degenerative brain disease diagnosis: Dementia biomarkers detected by DNA aptamer-attached portable graphene biosensor DOI Creative Commons
Tyler Bodily, Anirudh Ramanathan,

Shanhong Wei

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(47)

Published: Nov. 13, 2023

Dementia is a brain disease which results in irreversible and progressive loss of cognition motor activity. Despite global efforts, there no simple reliable diagnosis or treatment option. Current involves indirect testing commonly inaccessible biofluids low-resolution imaging. We have developed portable, wireless readout-based Graphene field-effect transistor (GFET) biosensor platform that can detect viruses, proteins, small molecules with single-molecule sensitivity specificity. report the detection three important amyloids, namely, Amyloid beta (Aβ), Tau (τ), α-Synuclein (αS) using DNA aptamer nanoprobes. These amyloids were isolated, purified, characterized from autopsied tissues Alzheimer’s Disease (AD) Parkinson’s (PD) patients. The limit (LoD) sensor 10 fM, 1–10 pM, 10–100 fM for Aβ, τ, αS, respectively. Synthetic as well brain-derived showed statistically significant response respect to derived thresholds, confirming ability define diseased vs. nondiseased states. each amyloid was specific their aptamers; αS peptides when tested, respectively, aptamers nonspecific them insignificant cross-reactivity. Thus, aptamer-based GFET has high precision across range epidemiologically AD PD variants. This portable diagnostic system would allow at-home POC neurodegenerative diseases globally.

Language: Английский

Citations

11
Field effect transistor biosensors for healthcare monitoring DOI Creative Commons
Zhicheng Zhang, Jingjing Hu,

Shijun Lin

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

Abstract The burgeoning demand for healthcare monitoring has brought field effect transistor (FET) biosensors into the spotlight as a highly efficient detection technology. FET offer inherent advantages including high sensitivity, rapid response times, operational simplicity, integrability, and label‐free detection. These characteristics render them particularly well‐suited detecting diverse array of physiological parameters biomarkers, thereby furnishing real‐time data crucial personalized medicine disease prevention. This review aims to elucidate recent advancements in within realm management encompassing several facets. Initially, this systematically analyzes device architecture, sensing mechanisms, performance evaluation methods gain an in‐depth understanding their principles features. Subsequently, it focuses on application health‐related biomarkers nucleic acids, proteins, exosomes, viruses, etc. Lastly, presents engineered medical sensor prototypes predicated biosensors, such point‐of‐care testing devices, wearable sensors, implantable underscoring practical utility potential health management. In addition, addresses critical issues prospects using monitoring, aiming provide some references insights research innovation field.

Language: Английский

Citations

4
Revolutionizing SARS-CoV-2 omicron variant detection: Towards faster and more reliable methods DOI
Dan Li, Cai Sun,

Pengfei Zhuang

et al.

Talanta, Journal Year: 2023, Volume and Issue: 266, P. 124937 - 124937

Published: July 12, 2023

Language: Английский

Citations

11
Nanopot plasmonic sensor platform for broad spectrum virus detection DOI
Young-Kyu Hwang, Zhi‐Jun Zhao,

Sangho Shin

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159484 - 159484

Published: Jan. 1, 2025

Language: Английский

Citations

0
Protein Detection Based on Field-Effect Transistor Biosensors for Diagnosing Diseases DOI
Zhicheng Zhang, Haiyang Li, Ning Zhou

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Proteins have been one of the most important biomarkers for diagnosing diseases, and field-effect transistor (FET) biosensors possess high sensitivity; are label-free; feature real-time detection, rapidity, easy integration protein detection. FET mainly made up parts, such as channel materials, bio receptors. This Tutorial provides an in-depth exploration detection from composition perspective discusses commercialization point-of-care diagnostics proteins based on biosensors.

Language: Английский

Citations

0