Antibiofilm Effects of Plant Extracts Against Staphylococcus aureus

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 454 - 454

Published: Feb. 19, 2025

The global rise in antimicrobial resistance poses a significant threat to public health, necessitating alternative therapeutic options. One critical challenge is treating infections caused by biofilm-forming bacteria, which are notably resistant conventional antibiotics. Staphylococcus aureus, including methicillin-resistant strains (MRSA), major pathogen biofilm-related infections, complicating treatment and leading chronic cases. Plant extracts have emerged as promising alternatives, offering new avenues for effective treatment. This study evaluated the antibacterial antibiofilm activities of commercial Vitis vinifera L. (grape), Camellia sinensis (green tea), Olea europaea (olive), Quercus robur (oak), Coffea arabica (coffee) against S. aureus from ATCC collections clinical isolates. Preliminary screening using disk diffusion test assessed zones inhibition, was followed minimum inhibitory concentration (MIC) determination via broth microdilution, with showing best overall MIC results. results obtained demonstrate strong activity extracts, values ranging 0.2 12.4 mg/mL. Using XTT reduction assay, inhibited biofilm growth 80-85% after 24 h incubation, achieving interesting activities. These findings suggest that investigated plant hold potential agents inhibitors, an approach tackling resistance. Further research needed explore their applications developing novel adjuvant therapies.

Language: Английский

---

Global challenges and microbial biofilms: Identification of priority questions in biofilm research, innovation and policy

Biofilm, Journal Year: 2024, Volume and Issue: 8, P. 100210 - 100210

Published: July 4, 2024

Priority question exercises are increasingly used to frame and set future research, innovation development agendas. They can provide an important bridge between the discoveries, data outputs generated by researchers, information required policy makers funders. Microbial biofilms present huge scientific, societal economic opportunities challenges. In order identify key priorities that will help advance field, here we review questions from a pool submitted international biofilm research community practitioners working across industry, environment medicine. To avoid bias computational approaches group manage voting selection process. The outcome of exercise is 78 unique questions, categorized in six themes: (i) Biofilm control, disruption, prevention, management, treatment (13 questions); (ii) Resistance, persistence, tolerance, role aggregation, immune interaction, relevance infection (10 (iii) Model systems, standards, regulatory, education, interdisciplinary (15 (iv) Polymicrobial, interactions, ecology, microbiome, phage (v) Clinical focus, chronic infection, detection, diagnostics (vi) Matrix, lipids, capsule, metabolism, development, physiology, evolution environment, engineering (14 questions). presented intended highlight opportunities, stimulate discussion focus for funders makers, informing strategy microbial communities.

Language: Английский

---

Nanomedicine against biofilm infections: A roadmap of challenges and limitations

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2024, Volume and Issue: 16(1)

Published: Jan. 1, 2024

Abstract Microbial biofilms are complex three‐dimensional structures where sessile microbes embedded in a polymeric extracellular matrix. Their resistance toward the host immune system as well to diverse range of antimicrobial treatments poses serious health and development threat, being top 10 global public threats declared by World Health Organization. In an effort combat biofilm‐related microbial infections, several strategies have been developed independently eliminate or complement conventional antibiotic therapies. However, their limitations leave room for other treatment alternatives, application nanotechnology biofilm eradication has gained significant relevance recent years. small size, penetration efficiency, design flexibility that they present makes them promising alternative infection treatment, although also set‐backs. This review aims describe main possibilities nanomedicine against biofilms, while covering aspects formation study, current therapies treatment. article is categorized under: Therapeutic Approaches Drug Discovery > Nanomedicine Infectious Disease Toxicology Regulatory Issues Nanomaterials Policy

Language: Английский

---

Antibiotic resistance and biofilm formation of thermoduric Kocuria and Micrococci isolated from the milk samples of healthy cows

DELETED, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Language: Английский

---

The burden of antimicrobial resistance in biofilm forming Staphylococcus spp. from Vernal Keratoconjunctivitis patients eyes

Biofilm, Journal Year: 2025, Volume and Issue: unknown, P. 100278 - 100278

Published: April 1, 2025

Vernal keratoconjunctivitis (VKC) is a chronic allergic ocular surface disease with seasonal recurrences and severe forms showing vision threatening complications. The purpose of the study to understand prevalence diversity biofilm-forming bacteria antimicrobial resistance in VKC compared healthy individuals (HC). For this, conjunctival swab samples were collected from (n = 26) HC 23), which culture positive 77 % 78.26 respectively. 16S rRNA gene sequencing revealed significant increase bacterial (p < 0.05), identifying 16 9 species, Staphylococcus epidermidis emerged as predominant bacterium both groups, relative abundances 52.8 30.2 0.001). Biofilm formation was observed 64.15 species 31 Scanning electron microscopy analysis confirmed temporal biofilm by spp. groups. Minimum inhibitory concentration testing showed that forming exhibited multidrug (>2 antibiotics) more frequently than those HC. Additionally, demonstrated higher fluoroquinolones These findings indicate significantly greater resistant Patients

Language: Английский

---

A pH‐responsive Cascade Nano‐Reactor Elevates ROS Generation by Remodeling Biofilm Microenvironment for Enhanced Antibacterial Treatment

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Biofilms are the root of most chronic and persistent infections pose a significant threat to human health. Reactive oxygen species (ROS) generation platforms have been used combat biofilm‐associated infections. However, biofilm microenvironments (BME) such as hypoxia overexpressed antioxidants restrict efficacy ROS‐based therapies. To address problem, this study incorporates calcium peroxide (CaO 2 ) berberine (BBR) into Fe Zn containing bimetal metal–organic frameworks (FZ) construct composite ROS nanogenerator (CBFZ), which is able remodel BME further promotes for enhance eradication. CBFZ degrades release CaO , 3+ 2+, BBR in biofilm, where decomposes O H relieve hypoxia, consumes glutathione (GSH). Subsequently, remodeled boosts production ‐dependent BBR‐mediated photodynamic therapy 2+ ‐based chemodynamic therapy, depleted GSH minimizes scavenging meantime, ultimately maintaining high level biofilm. It demonstrated that can effectively eradicate by killing embedded bacteria dispersing matrix. Moreover, exhibits an outstanding therapeutic effect murine model with subcutaneous infection. Overall, work offers propagable strategy antibiofilm therapy.

Language: Английский

---

Repurposing Mouthwashes: Antifungal and Antibiofilm Abilities of Commercially Available Mouthwashes Against Candida spp.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 483 - 483

Published: May 9, 2025

Background/Objectives: The main objective was to evaluate and compare the antifungal efficacy against Candida spp. in commercially available mouthwashes distributed European market. Indeed, solution emerging infectious diseases may no longer lie costly new drug development but rather unlocking untapped potential of existing substances. Materials Methods: Eighteen mouthwashes, chosen based on their composition, were tested vitro ten strains, including clinical isolates oral origin reference both planktonic biofilm forms. susceptibility testing conducted using Committee Antimicrobial Susceptibility Testing (EUCAST) disc diffusion method evaluation kinetic growth Candida. Biofilm reduction determined by minimal eradication concentration (MBEC). Scanning electron microscopy (SEM) analysis performed morphological alterations biofilms. Results: Most effectively reduced biomass production colony-forming unit (CFU) count. Parodontax Extra showed highest efficacy. In assay, Gum Paroex 0.12% exhibited largest average inhibition zone diameter. Some unusual trends data be explained a higher reaction fungal cells release excess during co-incubation mouthwashes. SEM images revealed significant alterations. Conclusion: Mouthwashes containing chlorhexidine digluconate, either alone or combination with cetylpyridinium chloride other active compounds, emerged as common factor among most efficacious formulations. vivo studies will essential validate these findings, serve valuable adjuvant treatment candidiasis.

Language: Английский

---

Ultrasound‐Activated Selenium Nanocarrier: Bactericidal Enhancement and Osseointegration Promotion for Implant‐Associated Infections

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Abstract Implant‐associated infections (IAIs) are common and challenging complications of orthopedic surgery. The physical barrier formed by biofilms the antioxidant defense system bacteria shield them from attack antimicrobial agents immune cells, leading to irreversible bone loss failure osseointegration. To address these challenges enhance osseointegration in presence biofilm infections, a sequential therapy strategy is proposed using an ultrasound‐activated nanocarrier, PLGA@H/Se, designed disrupt bacterial defenses subsequently enhancing osteogenic differentiation. As expected, when activated ultrasound, induces cavitation effect that disrupts outer biofilm, while promoting deep delivery encapsulated SeNPs peptide HHC‐36. target internal H₂S‐based bacteria, thereby synergistically bactericidal Furthermore, sustained release regulates selenoprotein expression, boosts stress responses, activates Wnt/β‐catenin pathway, which helps restore differentiation potential BMSCs impaired oxidative damage, both vitro vivo. Collectively, this ultrasound‐based facilitates functional under pathological conditions, offering practical comprehensive for treating IAIs.

Language: Английский

---

Synergistic Activity of Vancomycin and Gentamicin Against Staphylococcus aureus Biofilms on Polyurethane Surface

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 1119 - 1119

Published: May 13, 2025

Background: Staphylococcus aureus are frequently associated with biofilm formation on intravascular devices. Biofilms limit antimicrobial penetration and promote phenotypic resistance, challenging conventional treatment strategies. Vancomycin (VAN) gentamicin (GEN) have been used clinically, but their combined antibiofilm activity remains underexplored. Objectives: This study evaluates the efficacy of VAN GEN, alone in combination, against biofilms formed by methicillin-resistant (MRSA) methicillin-sensitive S. (MSSA) polyurethane. Methods: MICs were determined for GEN. Biofilm biomass metabolic quantified using crystal violet MTT assays, respectively. viability was assessed through fluorescence microscopy a modified Calgary Device. A continuous-flow peristaltic model developed to test under simulated catheter conditions. Results: While monotherapy or GEN had modest effects, combination significantly reduced activity. 20 mg/L + 8 40 achieved over 70% reduction MRSA complete eradication MBEC assays. Dynamic assays confirmed therapy. Conclusion: The VAN/GEN exhibits synergistic aureus, particularly MRSA. These findings support its potential application salvage strategies, including antibiotic lock

Language: Английский

---

Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing

npj Antimicrobials and Resistance, Journal Year: 2025, Volume and Issue: 3(1)

Published: May 21, 2025

Language: Английский

---