The pivotal roles of gut microbiota in insect plant interactions for sustainable pest management

npj Biofilms and Microbiomes, Journal Year: 2023, Volume and Issue: 9(1)

Published: Sept. 21, 2023

The gut microbiota serves as a critical "organ" in the life cycle of animals, particularly intricate interplay between herbivorous pests and plants. This review summarizes pivotal functions mediating insect-plant interactions, encompassing their influence on host insects, modulation plant physiology, regulation third trophic level species within ecological network. Given these significant functions, it is plausible to harness interactions underlying mechanisms develop novel eco-friendly pest control strategies. In this context, we also outline some emerging methods based intestinal or bacteria-mediated such symbiont-mediated RNAi paratransgenesis, albeit are still nascent stages confront numerous challenges. Overall, both opportunities challenges coexist exploration microbiota-mediated insect plants, which will not only enrich fundamental knowledge plant-insect but facilitate development sustainable

Language: Английский

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Enhanced capacity of a leaf beetle to combat dual stress from entomopathogens and herbicides mediated by associated microbiota

Integrative Zoology, Journal Year: 2024, Volume and Issue: 19(6), P. 1092 - 1104

Published: Feb. 20, 2024

Herbicides have demonstrated their impact on insect fitness by affecting associated microbiota or altering the virulence of entomopathogenic fungi toward insects. However, limited research has explored implications herbicide stress intricate tripartite interaction among insects, bacterial communities, and entomopathogens. In this study, we initially that bacteria confer a leaf beetle, Plagiodera versicolora, with capability to resist fungus Aspergillus nomius infection, sustained even under glyphosate stress. Further analysis revealed significant alteration in abundance composition due treatment. The dominant bacterium, post A. infection following combination treatments, exhibited strong suppressive effects fungal growth. Additionally, markedly inhibited pathogenic bacterium Pseudomonas though it P. versicolora's immunity, ultimately enhancing beetle's tolerance nomius. summary, our findings suggest bestow an augmented resilience against dual stressors both entomopathogen glyphosate. These results provide insight into residues interactions bacteria, fungi, holding for pest control ecosystem assessment.

Language: Английский

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Insights into plant–microbe interactions in the rhizosphere to promote sustainable agriculture in the new crops era

New Crops, Journal Year: 2023, Volume and Issue: 1, P. 100004 - 100004

Published: Nov. 18, 2023

Microbes accompany plants throughout their lives, from the seed to ripe fruit. Plant-microbe interactions have long been a focus of research, leading thousands articles that demonstrate importance for agriculture. Here, we review these previous findings, and discuss future directions prospects application plant-microbe various perspectives: community composition, pathways interaction, influencing factors interactions, methods techniques studying potential applications interactions. We propose exploiting utilizing core beneficial microbes, artificial assembly microbiota, in situ regulation microbiome function will all become essential aspects

Language: Английский

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Unraveling plant–microbe interactions: can integrated omics approaches offer concrete answers?

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 75(5), P. 1289 - 1313

Published: Nov. 9, 2023

Abstract Advances in high throughput omics techniques provide avenues to decipher plant microbiomes. However, there is limited information on how integrated informatics can help deeper insights into plant–microbe interactions a concerted way. Integrating multi-omics datasets transform our understanding of the microbiome from unspecified genetic influences interacting species specific gene-by-gene interactions. Here, we highlight recent progress and emerging strategies crop research review key aspects integration host microbial omics-based be used comprehensive outline complex crop–microbe We describe these technological advances have helped unravel crucial genes pathways that control beneficial, pathogenic, commensal identify knowledge gaps synthesize current limitations approaches. studies which multi-omics-based approaches led improved models community structure function. Finally, recommend holistic integrating achieve precision efficiency data analysis, for biotic abiotic stress contribution microbiota shaping fitness.

Language: Английский

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Heat Stress and Plant–Biotic Interactions: Advances and Perspectives

Plants, Journal Year: 2024, Volume and Issue: 13(15), P. 2022 - 2022

Published: July 23, 2024

Climate change presents numerous challenges for agriculture, including frequent events of plant abiotic stresses such as elevated temperatures that lead to heat stress (HS). As the primary driving factor climate change, HS threatens global food security and biodiversity. In recent years, have negatively impacted physiology, reducing plant's ability maintain disease resistance resulting in lower crop yields. Plants must adapt their priorities toward defense mechanisms tolerate challenging environments. Furthermore, selective breeding long-term domestication higher yields made varieties vulnerable multiple stressors, making them more susceptible events. Studies on predict concurrent biotic will become severe future, potentially occurring simultaneously or sequentially. While most studies focused singular effects systems examine how plants respond specific stresses, simultaneous occurrence pose a growing threat agricultural productivity. Few explored interactions between plant-biotic interactions. Here, we aim shed light physiological molecular (bacteria, fungi, oomycetes, nematodes, insect pests, pollinators, weedy species, parasitic plants), well combined impact growth We also advances designing developing various strategies address multi-stress scenarios related factors.

Language: Английский

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Entomopathogenic Fungus-Related Priming Defense Mechanisms in Cucurbits Impact Spodoptera littoralis (Boisduval) Fitness

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(8)

Published: July 13, 2023

Entomopathogenic fungi (EPF) exhibit direct and indirect mechanisms to increase plant resistance against biotic abiotic stresses. Plant responses these stresses are interconnected by common regulators such as ethylene (ET), which is involved in both iron (Fe) deficiency induced systemic responses. In this work, the roots of cucurbit seedlings were primed with Metarhizium brunneum (EAMa 01/58-Su strain), relative expression levels 18 genes related jasmonic acid (JA), salicylic (SA) synthesis, well pathogen-related (PR) protein genes, studied reverse transcription-quantitative PCR (qRT-PCR). Effects priming on Spodoptera littoralis feeding larvae for 15 days control plants. Genes showed upregulation species; however, highest was observed shoots plants Fe deficiency, demonstrating complexity overlapping degree regulatory network.

Language: Английский

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The sophisticated evolution of Trichoderma to control insect pests

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(12)

Published: March 13, 2023

Microbial communities are found throughout the biosphere, from human guts to glaciers, soil activated sludge. Understanding statistical properties of such diverse can pave way elucidate common mechanisms ...Multiple ecological forces act together shape composition microbial communities. Phyloecology approaches—which combine phylogenetic relationships between species with community ecology—have potential disentangle but often ...

Language: Английский

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Plant growth-promoting bacteria potentiate antifungal and plant-beneficial responses of Trichoderma atroviride by upregulating its effector functions

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(3), P. e0301139 - e0301139

Published: March 22, 2024

Trichoderma uses different molecules to establish communication during its interactions with other organisms, such as effector proteins. Effectors modulate plant physiology colonize roots or improve ’s mycoparasitic capacity. In the soil, these fungi can relationships growth–promoting bacteria (PGPBs), thus affecting their overall benefits on fungal prey, and possibly, role of The aim this study was determine induction atroviride gene expression coding for proteins interaction PGPBs, Arabidopsis phytopathogen Fusarium brachygibbosum , whether PGPBs potentiates beneficial effects T . During F genes epl1 tatrx2 tacfem1 increased expression, especially consortia bacteria. increased, mainly consortium formed Pseudomonas fluorescens UM270, Bacillus velezensis AF12, B halotolerans AF23. Additionally, by R badensis SER3 stimulated A thaliana PR1:GUS LOX2:GUS SA- JA-mediated defence responses. Finally, better at inhibiting pathogen growth, but UM270 promoting growth. These results showed that biocontrol capacity growth-promoting traits spp. be potentiated stimulating functions.

Language: Английский

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Gut bacteria of lepidopteran herbivores facilitate digestion of plant toxins

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(42)

Published: Oct. 11, 2024

Lepidopterans commonly feed on plant material, being the most significant insect herbivores in nature. Despite resistance to herbivory, such as producing toxic secondary metabolites, have developed mechanisms encoded their genomes tolerate or detoxify defensive compounds. Recent studies also highlight role of gut microbiota mediating detoxification herbivores; however, convincing evidence supporting contribution symbionts is rare Lepidoptera. Here, we show that growth various lepidopteran species was inhibited by a mulberry-derived metabolite, 1-deoxynojirimycin (DNJ); expected, specialist silkworm Bombyx mori grew well, but interestingly, early-instar silkworms affected DNJ level, and several bacterial responded positively enriched DNJ. Among these, strain isolated from ( Pseudomonas fulva ZJU1) can degrade utilize sole energy source, after inoculation into nonspecialists (e.g., beet armyworm Spodoptera exigua ), P. ZJU1 increased host significantly promoted growth. We used genomic transcriptomic analyses identify genes potentially involved degradation, CRISPR-Cas9-mediated mutagenesis verified function ilvB , key binding protein, metabolizing Furthermore, deletion mutant, exhibiting normal growth, could no longer enhance nonspecialist performance, degradation vivo. Therefore, our study demonstrated causality between microbiome chemical defense Lepidoptera, facilitating mechanistic understanding host–microbe relationships across this complex, abundant group.

Language: Английский

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The Sordariomycetes: an expanding resource with Big Data for mining in evolutionary genomics and transcriptomics

Frontiers in Fungal Biology, Journal Year: 2023, Volume and Issue: 4

Published: June 30, 2023

Advances in genomics and transcriptomics accompanying the rapid accumulation of omics data have provided new tools that transformed expanded traditional concepts model fungi. Evolutionary flourished with use classical newer fungal models facilitate study diverse topics encompassing biology development. Technological advances also created opportunity to obtain mine large datasets. One such continuously growing dataset is Sordariomycetes, which exhibit a richness species, ecological diversity, economic importance, profound research history on amenable models. Currently, 3,574 species this class been sequenced, comprising nearly one-third available ascomycete genomes. Among these genomes, multiple representatives genera

Language: Английский

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