Comparative genomics identifies key adaptive traits of sponge‐associated microbial symbionts DOI Creative Commons
Paul A. O’Brien, Steven J. Robbins, Shangjin Tan

et al.

Environmental Microbiology, Journal Year: 2024, Volume and Issue: 26(9)

Published: Sept. 1, 2024

Abstract Sponge microbiomes are often highly diverse making it difficult to determine which lineages important for maintaining host health and homeostasis. Characterising genomic traits associated with symbiosis can improve our knowledge of have adapted their what functions they might provide. Here we examined five microbial families sponges that previously shown evidence cophylogeny, including Endozoicomonadaceae, Nitrosopumilaceae, Spirochaetaceae, Microtrichaceae Thermoanaerobaculaceae , better understand the mechanisms behind symbiosis. We compared sponge‐associated genomes found in other environments sponge‐specific clades were enriched genes encoding many known symbiont survival, such as avoiding phagocytosis defence against foreign genetic elements. expand on previous show glycosyl hydrolases sulfatases sulfotransferases likely form multienzyme degradation pathways break remodel sulfated polysaccharides reveal an enrichment superoxide dismutase may prevent damage from free oxygen radicals produced by host. Finally, identified novel symbionts, urea metabolism Spirochaetaceae was be rare phylum Spirochaetota. These results identify putative symbionts living association sponges.

Language: Английский

Reactive oxygen species are regulated by immune deficiency and Toll pathways in determining the host specificity of honeybee gut bacteria DOI Creative Commons

Lizhen Guo,

Junbo Tang, Min Tang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(33)

Published: Aug. 9, 2023

Host specificity is observed in gut symbionts of diverse animal lineages. But how hosts maintain while rejecting their close relatives remains elusive. We use eusocial bees and codiversified bacteria to understand host regulation driving symbiotic specificity. The cross-inoculation bumblebee Gilliamella induced higher prostaglandin the honeybee gut, promoting a pronounced response through immune deficiency (IMD) Toll pathways. Gene silencing vitamin C treatments indicate that reactive oxygen species (ROS), not antimicrobial peptides, acts as effector inhibiting non-native strain. Quantitative PCR RNAi further reveal regulatory function IMD pathways, which Relish dorsal-1 may regulate Dual Oxidase ( Duox ) for ROS production. Therefore, maintains by creating hostile environment exotic bacteria, differential its system, reflecting co-opting existing machinery evolved combat pathogens.

Language: Английский

Citations

34
Strain diversity and host specificity of the gut symbiont Gilliamella in Apis mellifera, Apis cerana and Bombus terrestris DOI

Nihong Zhou,

Qi Zheng,

Yao Liu

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 293, P. 128048 - 128048

Published: Jan. 6, 2025

Language: Английский

Citations

1
A review on recent taxonomic updates of gut bacteria associated with social bees, with a curated genomic reference database DOI
Chengfeng Yang,

Jiawei Hu,

Qinzhi Su

et al.

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: April 9, 2024

Honeybees and bumblebees play a crucial role as essential pollinators. The special gut microbiome of social bees is key factor in determining the overall fitness health host. Although harbor relatively simple microbial communities at genus level, recent studies have unveiled significant genetic divergence variations gene content within each bacterial genus. However, comprehensive refined genomics-based taxonomic database specific to bee microbiomes remains lacking. Here, we first provided an overview current knowledge on distribution function bacteria, well factors that influence population dynamics. We then consolidated all available genomes bacteria species-level taxonomy, by constructing maximum-likelihood core genome phylogeny calculating genome-wide pairwise average nucleotide identity. On basis species constructed curated genomic reference database, named microbe sequence (BGM-GDb). To evaluate species-profiling performance BGM-GDb, retrieved series metagenomic data inferred composition using intra-species diversity analysis system (MIDAS), compared results with those obtained from prebuilt MIDAS database. found default BGM-GDb excelled aligned read counts richness. Overall, this high-resolution precise will facilitate research understanding community structure bees.

Language: Английский

Citations

4
Temporospatial dynamics and host specificity of honeybee gut bacteria DOI Creative Commons
Shiqi Luo, Xue Zhang, Xin Zhou

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(7), P. 114408 - 114408

Published: June 26, 2024

Honeybees are important pollinators worldwide, with their gut microbiota playing a crucial role in maintaining health. The bacteria of honeybees consist primarily five core lineages that spread through social interactions. Previous studies have provided basic understanding the composition and function honeybee microbiota, recent advancements focusing on analyzing diversity at strain level changes bacterial functional genes. Research across different regions globally has insights into microbial ecology. Additionally, findings shed light mechanisms host specificity bacteria. This review explores temporospatial dynamics discussing reasons behind these fluctuations. synopsis provides host-microbe interactions is invaluable for

Language: Английский

Citations

4
Type VI secretion systems promote intraspecific competition and host interactions in a bee gut symbiont DOI Creative Commons
Erick V. S. Motta, Patrick J. Lariviere, Korin Rex Jones

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(44)

Published: Oct. 23, 2024

The Type VI Secretion System (T6SS) is a sophisticated mechanism utilized by gram-negative bacteria to deliver toxic effector proteins into target cells, influencing microbial community dynamics and host interactions. In this study, we investigated the role of T6SSs in Snodgrassella alvi wkB2, core bacterial symbiont honey bee gut microbiota. We generated single- double-knockout mutants targeting essential genes ( tssD tssE ) both T6SS-1 T6SS-2 assessed their colonization competition capabilities vivo. Our results indicate that are nonessential for gut, although mutant strains exhibited significantly lower levels compared wild-type (WT) strain. Further, defined experiment showed S. wkB2 do not impact interspecific among bacteria. However, cocolonization experiments with closely related demonstrated plays mediating intraspecific competition. Transcriptomic analysis guts monocolonized WT or T6SS revealed differential expression immunity-related relative microbiota-deprived bees, such as upregulation antimicrobial peptide apidaecin presence defensin , suggesting contribute shaping immune responses. These findings provide insight ecological roles microbiota, emphasizing importance maintaining competitive host–bacterial

Language: Английский

Citations

4
Effects of Silibinin on Delaying Aging in Drosophila melanogaster DOI Creative Commons
Kai Zhu,

Hang Ni,

Eqra Hafeez

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 147 - 147

Published: Jan. 27, 2025

Aging is an inevitable physiological process, but delaying aging has always been enduring human pursuit. Silibinin (SIL), derived from the seeds of milk thistle plant, exhibits a broad spectrum pharmacological properties, including anti-tumor effects, liver protection, inhibition apoptosis, and alleviation inflammation. However, whether it anti-aging effects remains unclear. The SIL dietary supplement to Drosophila melanogaster prolonged lifespan, improved climbing ability, ameliorated age-associated intestinal barrier disruption, enhanced resistance oxidative stress, increased enzyme activities superoxide dismutase (SOD) catalase (CAT). Furthermore, RNA-seq results showed that addition significantly upregulated 74 genes downregulated 50 compared with control. KEGG (Kyoto Encyclopedia genomes) analysis demonstrated these differentially expressed were primarily involved in Toll signaling pathway endoplasmic reticulum proteins processing, six among which, IM2, IM3, Drsl3, CG7556, GCS1, TRAM, particularly regulation by supplementation. indicate enhancing antioxidant capacity regulating aging-related pathways. Therefore, shows potential application regimens.

Language: Английский

Citations

0
Chinese sacbrood virus mediates m6A modification to target and suppress the expression of hemolymph maintenance gene AF9, exacerbating bee infections DOI
Hua Bai,

Yueyu Ma,

Huitong Qiu

et al.

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

ABSTRACT The Chinese sacbrood virus (CSBV) severely threatens the beekeeping industry, wherein 3- to 5-day-old larvae in critical differentiation stage are highly susceptible low levels of CSBV exposure. Once infected, cannot undergo normal pupation, but pathogenic mechanism remains unclear. Previous studies have shown that m6A modification plays an important regulatory role larval development during stage. However, it is unknown whether infection affects pupation honeybee by altering modification. Here, a novel immunoregulatory factor, AF9, was identified through combined methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-seq analysis following infection. Enzyme-linked immunosorbent assay (ELISA) quantification methylation total MeRIP-qPCR further revealed inhibits expression AF9 via modification, thereby hindering host innate immune response promoting replication. then used demonstrate AcMETTL3 targets modifies mRNA, inhibiting expression. Homology functional human-derived (MLLT3) suggested exerted similar effect as MLLT3 on hemolymph functioning. dsRNA fed silence genes, followed extraction from hemolymph. Downregulation led decreased numbers live cells reduction phenoloxidase activity, response. Finally, Apis mellifera model constructed explore antiviral activities associated with AmAF9. AmAF9 similarly significant against deformed wing (DWV) acute bee paralysis (ABPV) infections pupae. These results indicate promotes overall targeting, leading immunosuppression exacerbating Similarly, stably expressed exhibits same effect, making broad-spectrum target viruses. IMPORTANCE poses serious threat health cerana colonies, yet its specific This study shows can enhance suppress targeting AcMETTL3, Further analyses indicated functions mammalian homologous gene maintaining Moreover, also significantly inhibit common In summary, new detailed here which escapes host’s enhancing . provides insights into mechanisms viruses responses suggests may serve potential bees.

Language: Английский

Citations

0
The multifaceted roles of gut microbiota in insect physiology, metabolism, and environmental adaptation: implications for pest management strategies DOI

Kamran Haider,

Dilawar Abbas, José Galián

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(3)

Published: Feb. 27, 2025

Language: Английский

Citations

0
Bacterial-induced Duox-ROS regulates the Imd immune pathway in the gut by modulating the peritrophic matrix DOI Creative Commons

Shuai Bai,

Zhichao Yao, Zhaohui Cai

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(3), P. 115404 - 115404

Published: March 1, 2025

The Duox-reactive oxygen species (ROS) system and the immune deficiency (Imd) pathway play a major role in insect gut immunity. However, their interaction to accomplish an effective response is still unclear. Here, we show that Duox regulates peritrophic matrix (PM) further affects Imd pathogens Bactrocera dorsalis. This regulation requires nuanced ROS balance: low H2O2 increases PM permeability, while higher damages during infection. Importantly, found commensal bacteria ensured proper Duox-dependent production stability, thus preventing overactivation pathogens. We conclude bacteria-induced Duox-ROS crucial for maintaining structural homeostasis, PM, turn, activation protects intestinal epithelial cells. Thus, our study reveals crosstalk between barrier Imd-mediated antibacterial function ensure host defense gut.

Language: Английский

Citations

0
Lactic Acid Bacteria: A Probiotic to Mitigate Pesticide Stress in Honey Bee DOI

S. Pradeep,

JOHNSON THANGARAJ EDWARD Y.S,

A. Suganthi

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

Citations

0