Reactive oxygen species are regulated by immune deficiency and Toll pathways in determining the host specificity of honeybee gut bacteria

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(33)

Published: Aug. 9, 2023

Host specificity is observed in gut symbionts of diverse animal lineages. But how hosts maintain while rejecting their close relatives remains elusive. We use eusocial bees and codiversified bacteria to understand host regulation driving symbiotic specificity. The cross-inoculation bumblebee Gilliamella induced higher prostaglandin the honeybee gut, promoting a pronounced response through immune deficiency (IMD) Toll pathways. Gene silencing vitamin C treatments indicate that reactive oxygen species (ROS), not antimicrobial peptides, acts as effector inhibiting non-native strain. Quantitative PCR RNAi further reveal regulatory function IMD pathways, which Relish dorsal-1 may regulate Dual Oxidase ( Duox ) for ROS production. Therefore, maintains by creating hostile environment exotic bacteria, differential its system, reflecting co-opting existing machinery evolved combat pathogens.

Language: Английский

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Strain diversity and host specificity of the gut symbiont Gilliamella in Apis mellifera, Apis cerana and Bombus terrestris

Microbiological Research, Journal Year: 2025, Volume and Issue: 293, P. 128048 - 128048

Published: Jan. 6, 2025

Language: Английский

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Lactic Acid Bacteria: A Probiotic to Mitigate Pesticide Stress in Honey Bee

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

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Chinese sacbrood virus mediates m6A modification to target and suppress the expression of hemolymph maintenance gene AF9, exacerbating bee infections

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

ABSTRACT The Chinese sacbrood virus (CSBV) severely threatens the beekeeping industry, wherein 3- to 5-day-old larvae in critical differentiation stage are highly susceptible low levels of CSBV exposure. Once infected, cannot undergo normal pupation, but pathogenic mechanism remains unclear. Previous studies have shown that m6A modification plays an important regulatory role larval development during stage. However, it is unknown whether infection affects pupation honeybee by altering modification. Here, a novel immunoregulatory factor, AF9, was identified through combined methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-seq analysis following infection. Enzyme-linked immunosorbent assay (ELISA) quantification methylation total MeRIP-qPCR further revealed inhibits expression AF9 via modification, thereby hindering host innate immune response promoting replication. then used demonstrate AcMETTL3 targets modifies mRNA, inhibiting expression. Homology functional human-derived (MLLT3) suggested exerted similar effect as MLLT3 on hemolymph functioning. dsRNA fed silence genes, followed extraction from hemolymph. Downregulation led decreased numbers live cells reduction phenoloxidase activity, response. Finally, Apis mellifera model constructed explore antiviral activities associated with AmAF9. AmAF9 similarly significant against deformed wing (DWV) acute bee paralysis (ABPV) infections pupae. These results indicate promotes overall targeting, leading immunosuppression exacerbating Similarly, stably expressed exhibits same effect, making broad-spectrum target viruses. IMPORTANCE poses serious threat health cerana colonies, yet its specific This study shows can enhance suppress targeting AcMETTL3, Further analyses indicated functions mammalian homologous gene maintaining Moreover, also significantly inhibit common In summary, new detailed here which escapes host’s enhancing . provides insights into mechanisms viruses responses suggests may serve potential bees.

Language: Английский

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Type VI secretion systems promote intraspecific competition and host interactions in a bee gut symbiont

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(44)

Published: Oct. 23, 2024

The Type VI Secretion System (T6SS) is a sophisticated mechanism utilized by gram-negative bacteria to deliver toxic effector proteins into target cells, influencing microbial community dynamics and host interactions. In this study, we investigated the role of T6SSs in Snodgrassella alvi wkB2, core bacterial symbiont honey bee gut microbiota. We generated single- double-knockout mutants targeting essential genes ( tssD tssE ) both T6SS-1 T6SS-2 assessed their colonization competition capabilities vivo. Our results indicate that are nonessential for gut, although mutant strains exhibited significantly lower levels compared wild-type (WT) strain. Further, defined experiment showed S. wkB2 do not impact interspecific among bacteria. However, cocolonization experiments with closely related demonstrated plays mediating intraspecific competition. Transcriptomic analysis guts monocolonized WT or T6SS revealed differential expression immunity-related relative microbiota-deprived bees, such as upregulation antimicrobial peptide apidaecin presence defensin , suggesting contribute shaping immune responses. These findings provide insight ecological roles microbiota, emphasizing importance maintaining competitive host–bacterial

Language: Английский

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A review on recent taxonomic updates of gut bacteria associated with social bees, with a curated genomic reference database

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: April 9, 2024

Honeybees and bumblebees play a crucial role as essential pollinators. The special gut microbiome of social bees is key factor in determining the overall fitness health host. Although harbor relatively simple microbial communities at genus level, recent studies have unveiled significant genetic divergence variations gene content within each bacterial genus. However, comprehensive refined genomics-based taxonomic database specific to bee microbiomes remains lacking. Here, we first provided an overview current knowledge on distribution function bacteria, well factors that influence population dynamics. We then consolidated all available genomes bacteria species-level taxonomy, by constructing maximum-likelihood core genome phylogeny calculating genome-wide pairwise average nucleotide identity. On basis species constructed curated genomic reference database, named microbe sequence (BGM-GDb). To evaluate species-profiling performance BGM-GDb, retrieved series metagenomic data inferred composition using intra-species diversity analysis system (MIDAS), compared results with those obtained from prebuilt MIDAS database. found default BGM-GDb excelled aligned read counts richness. Overall, this high-resolution precise will facilitate research understanding community structure bees.

Language: Английский

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Temporospatial dynamics and host specificity of honeybee gut bacteria

Cell Reports, Journal Year: 2024, Volume and Issue: 43(7), P. 114408 - 114408

Published: June 26, 2024

Honeybees are important pollinators worldwide, with their gut microbiota playing a crucial role in maintaining health. The bacteria of honeybees consist primarily five core lineages that spread through social interactions. Previous studies have provided basic understanding the composition and function honeybee microbiota, recent advancements focusing on analyzing diversity at strain level changes bacterial functional genes. Research across different regions globally has insights into microbial ecology. Additionally, findings shed light mechanisms host specificity bacteria. This review explores temporospatial dynamics discussing reasons behind these fluctuations. synopsis provides host-microbe interactions is invaluable for

Language: Английский

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The adaptation of bumblebees to extremely high elevation associated with their gut microbiota

mSystems, Journal Year: 2024, Volume and Issue: 9(3)

Published: Feb. 8, 2024

ABSTRACT Bumblebees are among the most abundant and important pollinators for sub-alpine alpine flowering plant species in Northern Hemisphere, but little is known about their adaptations to high elevations. In this article, we focused on two bumblebee species, Bombus friseanus prshewalskyi , respective gut microbiota. The distributed through Hengduan Mountains of southwestern China, show replacement at different We performed genome sequencing based 20 worker bee samples each species. Applying evolutionary population genetics metagenomic approaches, detected genes under selection analyzed functional pathways between bumblebees microbes. found clear genetic differentiation host significant differences Species occurred both hosts bacteria ( Snodgrassella ) with an increase elevation. These extremely high-elevation evidence positive related diverse biological processes. Positively selected involved immune systems probably contributed microbiota changes, while butyrate generated by may influence energy metabolism systems. This suggests a close association genomes microbiomes some degree natural selection. IMPORTANCE Two closely dominant elevations showed genomic signature adaptation elevation molecular level Bumblebees’ higher associated processes: response. Information allowing us understand adaptive mechanisms extreme conditions implicit if conserve them as environments change.

Language: Английский

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A systemic study of cyenopyrafen in strawberry cultivation system: Efficacy, residue behavior, and impact on honeybees (Apis mellifera L.)

Environmental Pollution, Journal Year: 2024, Volume and Issue: 346, P. 123601 - 123601

Published: Feb. 17, 2024

Language: Английский

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Effects of abamectin nanocapsules on bees through host physiology, immune function, and gut microbiome

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 930, P. 172738 - 172738

Published: April 24, 2024

Language: Английский

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