Microbiome, Journal Year: 2024, Volume and Issue: 12(1)
Published: Dec. 21, 2024
Language: Английский
Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(33)
Published: Aug. 9, 2023
Host specificity is observed in gut symbionts of diverse animal lineages. But how hosts maintain while rejecting their close relatives remains elusive. We use eusocial bees and codiversified bacteria to understand host regulation driving symbiotic specificity. The cross-inoculation bumblebee Gilliamella induced higher prostaglandin the honeybee gut, promoting a pronounced response through immune deficiency (IMD) Toll pathways. Gene silencing vitamin C treatments indicate that reactive oxygen species (ROS), not antimicrobial peptides, acts as effector inhibiting non-native strain. Quantitative PCR RNAi further reveal regulatory function IMD pathways, which Relish dorsal-1 may regulate Dual Oxidase ( Duox ) for ROS production. Therefore, maintains by creating hostile environment exotic bacteria, differential its system, reflecting co-opting existing machinery evolved combat pathogens.
Language: Английский
Citations
34
Microbiological Research, Journal Year: 2025, Volume and Issue: 293, P. 128048 - 128048
Published: Jan. 6, 2025
Language: Английский
Citations
2
Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown
Published: March 17, 2025
Language: Английский
Citations
2
Journal of Virology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 3, 2025
ABSTRACT The Chinese sacbrood virus (CSBV) severely threatens the beekeeping industry, wherein 3- to 5-day-old larvae in critical differentiation stage are highly susceptible low levels of CSBV exposure. Once infected, cannot undergo normal pupation, but pathogenic mechanism remains unclear. Previous studies have shown that m6A modification plays an important regulatory role larval development during stage. However, it is unknown whether infection affects pupation honeybee by altering modification. Here, a novel immunoregulatory factor, AF9, was identified through combined methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA-seq analysis following infection. Enzyme-linked immunosorbent assay (ELISA) quantification methylation total MeRIP-qPCR further revealed inhibits expression AF9 via modification, thereby hindering host innate immune response promoting replication. then used demonstrate AcMETTL3 targets modifies mRNA, inhibiting expression. Homology functional human-derived (MLLT3) suggested exerted similar effect as MLLT3 on hemolymph functioning. dsRNA fed silence genes, followed extraction from hemolymph. Downregulation led decreased numbers live cells reduction phenoloxidase activity, response. Finally, Apis mellifera model constructed explore antiviral activities associated with AmAF9. AmAF9 similarly significant against deformed wing (DWV) acute bee paralysis (ABPV) infections pupae. These results indicate promotes overall targeting, leading immunosuppression exacerbating Similarly, stably expressed exhibits same effect, making broad-spectrum target viruses. IMPORTANCE poses serious threat health cerana colonies, yet its specific This study shows can enhance suppress targeting AcMETTL3, Further analyses indicated functions mammalian homologous gene maintaining Moreover, also significantly inhibit common In summary, new detailed here which escapes host’s enhancing . provides insights into mechanisms viruses responses suggests may serve potential bees.
Language: Английский
Citations
1
Insect Science, Journal Year: 2024, Volume and Issue: unknown
Published: April 9, 2024
Honeybees and bumblebees play a crucial role as essential pollinators. The special gut microbiome of social bees is key factor in determining the overall fitness health host. Although harbor relatively simple microbial communities at genus level, recent studies have unveiled significant genetic divergence variations gene content within each bacterial genus. However, comprehensive refined genomics-based taxonomic database specific to bee microbiomes remains lacking. Here, we first provided an overview current knowledge on distribution function bacteria, well factors that influence population dynamics. We then consolidated all available genomes bacteria species-level taxonomy, by constructing maximum-likelihood core genome phylogeny calculating genome-wide pairwise average nucleotide identity. On basis species constructed curated genomic reference database, named microbe sequence (BGM-GDb). To evaluate species-profiling performance BGM-GDb, retrieved series metagenomic data inferred composition using intra-species diversity analysis system (MIDAS), compared results with those obtained from prebuilt MIDAS database. found default BGM-GDb excelled aligned read counts richness. Overall, this high-resolution precise will facilitate research understanding community structure bees.
Language: Английский
Citations
5
Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(44)
Published: Oct. 23, 2024
The Type VI Secretion System (T6SS) is a sophisticated mechanism utilized by gram-negative bacteria to deliver toxic effector proteins into target cells, influencing microbial community dynamics and host interactions. In this study, we investigated the role of T6SSs in Snodgrassella alvi wkB2, core bacterial symbiont honey bee gut microbiota. We generated single- double-knockout mutants targeting essential genes ( tssD tssE ) both T6SS-1 T6SS-2 assessed their colonization competition capabilities vivo. Our results indicate that are nonessential for gut, although mutant strains exhibited significantly lower levels compared wild-type (WT) strain. Further, defined experiment showed S. wkB2 do not impact interspecific among bacteria. However, cocolonization experiments with closely related demonstrated plays mediating intraspecific competition. Transcriptomic analysis guts monocolonized WT or T6SS revealed differential expression immunity-related relative microbiota-deprived bees, such as upregulation antimicrobial peptide apidaecin presence defensin , suggesting contribute shaping immune responses. These findings provide insight ecological roles microbiota, emphasizing importance maintaining competitive host–bacterial
Language: Английский
Citations
5
Cell Reports, Journal Year: 2024, Volume and Issue: 43(7), P. 114408 - 114408
Published: June 26, 2024
Honeybees are important pollinators worldwide, with their gut microbiota playing a crucial role in maintaining health. The bacteria of honeybees consist primarily five core lineages that spread through social interactions. Previous studies have provided basic understanding the composition and function honeybee microbiota, recent advancements focusing on analyzing diversity at strain level changes bacterial functional genes. Research across different regions globally has insights into microbial ecology. Additionally, findings shed light mechanisms host specificity bacteria. This review explores temporospatial dynamics discussing reasons behind these fluctuations. synopsis provides host-microbe interactions is invaluable for
Language: Английский
Citations
4
Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(36)
Published: Aug. 26, 2024
The gut microbiome plays an important role in honeybee hormonal regulation and growth, but the underlying mechanisms are poorly understood. Here, we showed that depletion of bacteria resulted reduced expression insulin-like peptide gene ( ilp ) head, accompanied by metabolic syndromes resembling those Type 1 diabetes humans: hyperglycemia, impaired lipid storage, decreased metabolism. These symptoms were alleviated bacterial inoculation. Gut metabolite profiling revealed succinate, produced Lactobacillus Firm-5, played deterministic roles activating regulating metabolism honeybees. Notably, demonstrated succinate modulates host through stimulating gluconeogenesis, a mechanism humans. This study presents evidence for modulating contributes to understanding interactions between bee hosts.
Language: Английский
Citations
3
Journal of Natural Pesticide Research, Journal Year: 2024, Volume and Issue: unknown, P. 100110 - 100110
Published: Dec. 1, 2024
Language: Английский
Citations
3
Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 147 - 147
Published: Jan. 27, 2025
Aging is an inevitable physiological process, but delaying aging has always been enduring human pursuit. Silibinin (SIL), derived from the seeds of milk thistle plant, exhibits a broad spectrum pharmacological properties, including anti-tumor effects, liver protection, inhibition apoptosis, and alleviation inflammation. However, whether it anti-aging effects remains unclear. The SIL dietary supplement to Drosophila melanogaster prolonged lifespan, improved climbing ability, ameliorated age-associated intestinal barrier disruption, enhanced resistance oxidative stress, increased enzyme activities superoxide dismutase (SOD) catalase (CAT). Furthermore, RNA-seq results showed that addition significantly upregulated 74 genes downregulated 50 compared with control. KEGG (Kyoto Encyclopedia genomes) analysis demonstrated these differentially expressed were primarily involved in Toll signaling pathway endoplasmic reticulum proteins processing, six among which, IM2, IM3, Drsl3, CG7556, GCS1, TRAM, particularly regulation by supplementation. indicate enhancing antioxidant capacity regulating aging-related pathways. Therefore, shows potential application regimens.
Language: Английский
Citations
0