Construction of a diagnostic model utilizing m7G regulatory factors for the characterization of diabetic nephropathy and the immune microenvironment

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 17, 2025

Diabetic nephropathy (DN), a prevalent and severe complication of diabetes, is associated with poor prognosis limited treatment options. N7-Methylguanosine (m7G) modification plays crucial role in regulating RNA structure function, linking it closely to metabolic disorders. However, despite its biological significance, the interplay between m7G methylation immune status DN remains largely unexplored. Leveraging data from GEO database, we conducted consensus clustering regulators patients identify distinct molecular subtypes. To construct validate m7G-related prognostic features risk scores, integrated multiple machine learning approaches, including Support Vector Machine-Recursive Feature Elimination, Random Forest, LASSO, Cox regression, ROC curves analysis. In addition, employed GSVA, ssGSEA, CIBERSORT, Gene Set Enrichment Analysis investigate pathways landscape, providing deeper insights into DN. Based on expression levels 18 regulatory factors, identified nine key regulators. Through techniques, four significant (METTL1, CYFIP2, EIF3D, NUDT4). Consensus classified these genes two clusters. characterize subtypes, infiltration analysis, differential enrichment uncovering differences Additionally, developed an scoring model using PCA algorithm. The was further validated through vivo experiments, reinforcing their potential disease progression. METTL1, NUDT4 may serve as diagnostic biomarkers for DN, new mechanisms landscape.

Language: Английский

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Mechanisms of gut microbiota-immune-host interaction on glucose regulation in type 2 diabetes

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 20, 2023

Intestinal absorption of food is one the sources glucose. Insulin resistance and impaired glucose tolerance caused by lifestyle diet are precursors type 2 diabetes. Patients with diabetes have trouble controlling their blood sugar levels. For long-term health, strict glycemic management necessary. Although it thought to be well correlated metabolic diseases like obesity, insulin resistance, diabetes, its molecular mechanism still not completely understood. Disturbed microbiota triggers gut immune response reshape homeostasis. This interaction only maintains dynamic changes intestinal flora, but also preserves integrity barrier. Meanwhile, establishes a systemic multiorgan dialog on gut-brain gut-liver axes, high-fat affects host’s feeding preference metabolism. Intervention in can combat decreased sensitivity linked both centrally peripherally. Moreover, pharmacokinetics oral hypoglycemic medications influenced microbiota. The accumulation drugs drug efficacy, composition function them, thus may help explain individual therapeutic variances pharmacological efficacy. Regulating through healthy dietary patterns or supplementing pro/prebiotics provide guidance for interventions people poor control. Traditional Chinese medicine used as complementary effectively regulate becoming new target against diseases, so more evidence needed elucidate intricate microbiota-immune-host relationship, explore potential targeting

Language: Английский

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Clinical Perspectives of Gut Microbiota in Patients with Chronic Kidney Disease and End-Stage Kidney Disease: Where Do We Stand?

Biomedicines, Journal Year: 2023, Volume and Issue: 11(9), P. 2480 - 2480

Published: Sept. 7, 2023

The gut microbiota (GM) plays a vital role in human health, with increasing evidence linking its imbalance to chronic kidney disease and end-stage disease. Although the exact methods underlying kidney-GM crosstalk are not fully understood, interventions targeting GM were made lay three aspects: diagnostic, predictive, therapeutic interventions. While these show promising results reducing uremic toxins inflammation, challenges remain form of patient-specific variability, potential side effects, safety concerns. Our understanding GMs is still evolving, necessitating further research elucidate causal relationship mechanistic interactions. Personalized focusing on specific signatures could enhance patient outcomes. However, comprehensive clinical trials needed validate approaches’ safety, efficacy, feasibility.

Language: Английский

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Pathogenesis and therapy of radiation enteritis with gut microbiota

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: March 31, 2023

Radiotherapy is widely used in clinic due to its good effect for cancer treatment. But radiotherapy of malignant tumors the abdomen and pelvis easy cause radiation enteritis complications. Gastrointestinal tract contains numerous microbes, most which are mutualistic relationship with host. Abdominal results gut microbiota dysbiosis. Microbial therapy can directly target reverse dysbiosis, hence relieving intestinal inflammation. In this review, we mainly summarized pathogenesis novel radiation-induced injury dysbiosis envision opportunities challenges therapy.

Language: Английский

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Molecular mechanisms of gut microbiota in diabetic nephropathy

Diabetes Research and Clinical Practice, Journal Year: 2024, Volume and Issue: 213, P. 111726 - 111726

Published: June 4, 2024

Language: Английский

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Effects of probiotics on non-alcoholic fatty liver disease: a review of human clinical trials

Frontiers in Nutrition, Journal Year: 2023, Volume and Issue: 10

Published: June 30, 2023

Non-alcoholic fatty liver disease (NAFLD) is a global public health issue, of which the prevalence about 25% worldwide. The incidence NAFLD increasing in patients with obesity, type 2 diabetes (T2DM) and metabolic syndrome. crosstalk between gut microbiota metabolism-related diseases has been raised great concern. Patients NAPLD were observed disruption microbiota. Several researches showed that was determination progression by experiments using fecal transplants. application probiotics, as one most important strategies for regulation disorder, have explored whether it beneficial to gut-related intestine-distal organs. Some probiotics improve parameters phenotype NAFLD. oral intake them might become effective management prevention treatment In this review, we summarized human clinical trials focusing on effects give some evidential reference administration

Language: Английский

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Inflammation and Oxidative Stress Induced by Obesity, Gestational Diabetes, and Preeclampsia in Pregnancy: Role of High-Density Lipoproteins as Vectors for Bioactive Compounds

Antioxidants, Journal Year: 2023, Volume and Issue: 12(10), P. 1894 - 1894

Published: Oct. 23, 2023

Inflammation and oxidative stress are essential components in a myriad of pathogenic entities that lead to metabolic chronic diseases. Moreover, inflammation its different phases is necessary for the initiation maintenance healthy pregnancy. Therefore, an equilibrium between necessary/pathologic level during pregnancy needed avoid disease development. High-density lipoproteins (HDL) important good neonatal outcome. Their role fetal development challenging situations vital maintaining equilibrium. However, certain conditions, such as obesity, diabetes, other cardiovascular diseases, it has been observed HDL loses protective properties, becoming dysfunctional. Bioactive compounds have widely studied mediators but their mechanisms action still unknown. Nonetheless, these agents, which obtained from functional foods, increase concentration HDL, TRC, antioxidant activity. this review first summarizes several participation stress. Second, gives insight into how may act vector bioactive compounds. Third, describes relationships process Consequently, databases were used, including MEDLINE, PubMed, Scopus, where scientific articles published English language up 2023 identified.

Language: Английский

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The role and mechanism of the gut microbiota in the development and treatment of diabetic kidney disease

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: April 21, 2023

Diabetic kidney disease (DKD) is a common complication in patients with diabetes mellitus (DM). Increasing evidence suggested that the gut microbiota participates progression of DKD, which involved insulin resistance, renin-angiotensin system (RAS) activation, oxidative stress, inflammation and immunity. Gut microbiota-targeted therapies including dietary fiber, supplementation probiotics or prebiotics, fecal transplantation diabetic agents modulate microbiota, such as metformin, glucagon-like peptide-1 (GLP-1) receptor agonists, dipeptidyl peptidase-4 (DPP-4) inhibitors, sodium-glucose transporter-2 (SGLT-2) inhibitors. In this review, we summarize most important findings about role pathogenesis DKD application therapies.

Language: Английский

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The role of probiotic supplementation in inflammatory biomarkers in adults: an umbrella meta-analysis of randomized controlled trials

Inflammopharmacology, Journal Year: 2023, Volume and Issue: 31(5), P. 2253 - 2268

Published: Sept. 12, 2023

Language: Английский

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Hypomagnesemia as a Risk Factor and Accelerator for Vascular Aging in Diabetes Mellitus and Chronic Kidney Disease

Metabolites, Journal Year: 2023, Volume and Issue: 13(2), P. 306 - 306

Published: Feb. 19, 2023

The age-old axiom that one is as old his or her vessels are, calls for ongoing critical re-examination of modifiable risk factors accelerated vascular ageing in chronic kidney diseases. Attempts to modulate with cholesterol-lowering agents have largely failed advanced disease (CKD). In addition nitrogen waste products, many pathological biochemical processes also play a role calcification damage. Magnesium, cation vital the body, may substantially reduce cardiovascular diseases’ and progression. This narrative review aimed address relationship between hypomagnesemia calcification, which promotes further complications diabetes, aging, CKD. Articles predefined keywords were searched PubMed Google Scholar databases specific inclusion exclusion criteria. We hypothesized decrease serum magnesium levels contributes increased thereby increases mortality. summary, based on existing evidence literature, it appears simple inexpensive oral supplementation mortality patients who are already severely affected by such diseases; this context, concept ‘normal’ vs. ‘ideal’ should be carefully re-examined.

Language: Английский

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