Unveiling Novel Insights in Helminth Proteomics: Advancements, Applications, and Implications for Parasitology and Beyond DOI Creative Commons

Nooshinmehr Soleymani,

Soheil Sadr, Cinzia Santucciu

et al.

Biologics, Journal Year: 2024, Volume and Issue: 4(3), P. 314 - 344

Published: Sept. 19, 2024

Helminths have developed intricate mechanisms to survive and evade the host’s immune responses. Hence, understanding excretory-secretory products (ESPs) by helminths is crucial for developing control tools, including drug targets, vaccines, potential therapies inflammatory metabolic disorders caused them. Proteomics, large-scale analysis of proteins, offers a powerful approach unravel complex proteomes gain insights into their biology. as science that delves functions has revolutionize clinical against parasitic infections anthelminthic resistance. Proteomic technologies lay framework accompanying genomic, reverse genetics, pharmacokinetic approaches provide more profound or broader coverage cellular underlie response anthelmintics. With development vaccines helminth infections, proteomics brought major change parasitology. The proteome can be analyzed comprehensively, revealing network proteins enable parasite survival pathogenicity. Furthermore, it reveals how parasites interact with hosts’ systems. current article reviews latest advancements in highlights valuable contributions search vaccines.

Language: Английский

Evaluation of the In-Vitro Effects of Albendazole, Mebendazole, and Praziquantel Nanocapsules against Protoscolices of Hydatid Cyst DOI Creative Commons

Nooshinmehr Soleymani,

Soheil Sadr, Cinzia Santucciu

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(9), P. 790 - 790

Published: Sept. 12, 2024

Cystic echinococcosis still remains a serious health and economic problem worldwide. The etiologic agent is Echinococcus granulosus sensu lato, giving origin to fluid-filled cystic lesion. Therapy faces several challenges. Nanodrugs have shown promise as chemotherapeutics against hydatid cysts. present study evaluated highly safe lipid nano-polymeric capsule for its superior efficacy ability overcome drug resistance. Nanocapsule drugs were formulated into six groups: Albendazole, mebendazole, praziquantel, albendazole + praziquantel mebendazole. protoscolicidal effects of these groups assessed at 10, 60, 120 min in three concentrations (1, 0.5, 0.25 mg/mL). Drug formulations via zeta potential, droplet size, solubility, particle size analyzer (PSA), scanning electron microscopy. According the PSA results, mean nanocapsules was 193.01 nm, mebendazole 170.40 180.44 nm. Albendazole showed greatest activity concentration 1 mg/mL after min. In contrast, each drug’s single-dose times least With right application nanotechnology, it possible produce effective drugs, such polymeric combination which has promising implications.

Language: Английский

Citations

4
Investigating the Therapeutic Effects of Albendazole, Mebendazole, and Praziquantel Nanocapsules in Hydatid Cyst-Infected Mice DOI Creative Commons

Nooshinmehr Soleymani,

Soheil Sadr, Cinzia Santucciu

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(3), P. 240 - 240

Published: March 1, 2025

Drug resistance is the main challenge in treating parasitic diseases, including cystic echinococcosis (CE). Hence, current study aims to investigate effect of nanocapsules containing albendazole (ABZ), mebendazole (MBZ), and praziquantel (PZQ) on hydatid cysts mice using these high-potency drugs. A total 78 female white laboratory (BALB/C mice), 8 weeks old weighing 25 g, were intraperitoneally injected with 1500 live protoscoleces Echinococcus granulosus. The first group received ABZ nanocapsules, 2 MBZ 3 PZQ 4 + 5 6 nanocapsules. Each also had a control group, which non-nanocapsulated drugs (group 7–12). Group 13 no treatment served as negative control, just receiving phosphate-buffered saline (PBS). thorough examination cysts’ physical properties, size, quantity, weight, was carried out. According our results, polymeric are sphere-like different sizes. number all nanocapsule groups significantly decreased compared group. In weight cysts, least cyst showing that use medicinal combination better penetration reduction cysts. conclusion, findings showed ABZ, MBZ, reduced mouse model used this study.

Language: Английский

Citations

0
An inclusive assessment of apoptosis mechanisms in Leishmania species: A narrative literature review DOI Creative Commons
Soheil Sadr, Iraj Sharifi, Solmaz Morovati

et al.

Current Research in Parasitology and Vector-Borne Diseases, Journal Year: 2025, Volume and Issue: unknown, P. 100260 - 100260

Published: April 1, 2025

Language: Английский

Citations

0
Advancing gut microbiome insights: Graphene quantum dot nanobiosensors for microbial metabolite detection DOI
Soheil Sadr,

Shakiba Nazemian,

Shiva Dianaty

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162954 - 162954

Published: April 1, 2025

Citations

0
Establishment of Echinococcus granulosus EgM123 Recombinant Gene Rabies Virus SRV9 and Identification of Its Biological Characteristics DOI Creative Commons

Yueqi Yang,

Mengdan Hou,

Guicheng Su

et al.

Viruses, Journal Year: 2024, Volume and Issue: 17(1), P. 30 - 30

Published: Dec. 28, 2024

Canids act as a crucial intermediary in the transmission of rabies and Echinococcus granulosus, serving co-infection hosts pathogen carriers for both hydatid disease (HD) transmitted from animals to humans. Therefore, an effective efficient bivalent oral vaccine preventing HD is urgently required reduce economic losses husbandry resulting HD. In this study, full-length plasmid (pcDNA4-NPM+GΔCD+EgM123+eGFP+L) carrying granulosus EgM123 gene fluorescence reporter genes eGFP four auxiliary transfection plasmids virus SRV9 (pcDNA4-N, pcDNA4-P, pcDNA4-G, pcDNA-L) were established by reverse genetics approaches co-transfected BSR cells electrotransfection. The showed green 48 h after recombinant was exposed sixth-generation blind passage, with N, P, G, amplified via RT-PCR, yielding targeted strips. rescued virus-infected characterized TEM, results indicated that bullet-like viral particles average size 148.47 nm cyst structure present cytoplasm cells; expression levels continuously cultivated 9th-, 10th-, 11th-, 12th-, 13th-generation viruses quantified qRT-PCR, mRNA upregulated. LD50 titer suckling rats measured be 10-1.4. synthesized can function strain development "Rabies-HD vaccine", therefore aiding prevention management animals.

Language: Английский

Citations

1
Unveiling Novel Insights in Helminth Proteomics: Advancements, Applications, and Implications for Parasitology and Beyond DOI Creative Commons

Nooshinmehr Soleymani,

Soheil Sadr, Cinzia Santucciu

et al.

Biologics, Journal Year: 2024, Volume and Issue: 4(3), P. 314 - 344

Published: Sept. 19, 2024

Helminths have developed intricate mechanisms to survive and evade the host’s immune responses. Hence, understanding excretory-secretory products (ESPs) by helminths is crucial for developing control tools, including drug targets, vaccines, potential therapies inflammatory metabolic disorders caused them. Proteomics, large-scale analysis of proteins, offers a powerful approach unravel complex proteomes gain insights into their biology. as science that delves functions has revolutionize clinical against parasitic infections anthelminthic resistance. Proteomic technologies lay framework accompanying genomic, reverse genetics, pharmacokinetic approaches provide more profound or broader coverage cellular underlie response anthelmintics. With development vaccines helminth infections, proteomics brought major change parasitology. The proteome can be analyzed comprehensively, revealing network proteins enable parasite survival pathogenicity. Furthermore, it reveals how parasites interact with hosts’ systems. current article reviews latest advancements in highlights valuable contributions search vaccines.

Language: Английский

Citations

0