Standardization of the Agar Plate Method for Bacteriophage Production DOI Creative Commons
Su Jin Jo, Young Min Lee,

K. K. Cho

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 14(1), P. 2 - 2

Published: Dec. 24, 2024

The growing threat of antimicrobial resistance (AMR), exacerbated by the COVID-19 pandemic, highlights urgent need for alternative treatments such as bacteriophage (phage) therapy. Phage therapy offers a targeted approach to combat bacterial infections, particularly those resistant conventional antibiotics. This study aimed standardize an agar plate method high-mix, low-volume phage production, suitable personalized Plaque assays were conducted with double-layer method, and plaque sizes precisely measured using image analysis tools. Regression models developed Minitab software established correlations between size optimizing production while minimizing development. resulting Size Calculation (PSC) model accurately correlated inoculum concentration yield, establishing specific plaque-forming unit (PFU) thresholds optimal production. Using phages targeting pathogens Escherichia, Salmonella, Staphylococcus, Pseudomonas, Chryseobacterium, Vibrio, Erwinia, Aeromonas confirmed model's accuracy across various conditions. validation showed strong inverse correlation minimum-lawn cell clearing PFUs (MCPs; R² = 98.91%) identified density that maximizes yield evolution mutants. These results highlight PSC standardized scalable efficient which is crucial AMR management. Furthermore, its adaptability different conditions positions it potential standard tool rapid precise screening propagation in both clinical industrial settings.

Language: Английский

Addressing the global challenge of bacterial drug resistance: insights, strategies, and future directions DOI Creative Commons
Arun Karnwal,

Amar Yasser Jassim,

Ameer A. Mohammed

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 24, 2025

The COVID-19 pandemic underscored bacterial resistance as a critical global health issue, exacerbated by the increased use of antibiotics during crisis. Notwithstanding pandemic's prevalence, initiatives to address medication have been inadequate. Although an overall drop in worldwide antibiotic consumption, total usage remains substantial, requiring rigorous regulatory measures and preventive activities mitigate emergence resistance. National Action Plans (NAPs) implemented worldwide, significant disparities persist, particularly low- middle-income countries (LMICs). Settings such farms, hospitals, wastewater treatment facilities, agricultural environments include presence Antibiotic Resistant Bacteria (ARB) antibiotic-resistance genes (ARG), promoting propagation Dietary modifications probiotic supplementation shown potential reshaping gut microbiota reducing gene prevalence. Combining with adjuvants or bacteriophages may enhance efficacy development. Novel therapeutic approaches, tailored antibiotics, monoclonal antibodies, vaccines, nanoparticles, offer alternate ways addressing In spite advancements next-generation sequencing analytics, gaps persist comprehending role regulating Effectively tackling requires robust policy interventions targeting root causes while minimizing public risks. This review provides information for developing strategies protocols prevent colonization, microbiome resilience, spread

Language: Английский

Citations

2
Recent advancements in bacterial anti-phage strategies and the underlying mechanisms altering susceptibility to antibiotics DOI
Hao Zou, Xiaoyi Huang, W. Xiao

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 295, P. 128107 - 128107

Published: Feb. 21, 2025

Language: Английский

Citations

1
Current strategies against multidrug-resistant Staphylococcus aureus and advances toward future therapy DOI Creative Commons
Saurabh Kumar, Richa Prasad Mahato,

Sanjay Ch

et al.

The Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 100281 - 100281

Published: Feb. 1, 2025

Language: Английский

Citations

1
Isolation, characterization, and genomic analysis of a novel bacteriophage vB_Kp_XP4 targeting hypervirulent and multidrug-resistant Klebsiella pneumoniae DOI Creative Commons
Xinya Peng,

Junliang Chang,

Hongxia Zhang

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

Introduction Hypervirulent and multidrug-resistant Klebsiella pneumoniae (hvKP MDR-KP) are significant public health threats. This study aimed to isolate a lytic bacteriophage targeting these high-risk strains, systematically characterize its biological properties, genomic features, therapeutic efficacy, establish foundation for clinical phage therapy novel antimicrobial development. Methods The vB_Kp_XP4 was isolated from river water using the double-layer agar plate method with clinically strain P4 as host. Morphology analyzed via transmission electron microscopy (TEM). Host range, pH, thermal stability were assessed spot assays OD 630 measurements. One-step growth curves determined latent period burst size. Whole-genome sequencing phylogenetic analysis performed. Therapeutic efficacy safety evaluated in Galleria mellonella infection model. Results TEM revealed Phage tailed an icosahedral head long, flexible tail. It lysed hvKP (carrying rmp , peg iuc iro genes) MDR-KP (resistant carbapenems, fluoroquinolones, etc.), optimal MOI of 0.1 <10 minutes. Stability maintained at pH 4–11 ≤70°C. linear double-stranded DNA genome 44,344 bp G+C content 53.80%. comprised 54 coding sequences lacked lysogenic, virulence, or antibiotic resistance genes. Phylogenetic positioned species within genus Drulisvirus family Autographiviridae . In model, prolonged survival P4-infected larvae ( P < 0.001) Conclusion exhibits high stability, specificity, potent activity, no undesirable genes, demonstrating effective vivo suggest potential applications against infections. presence multiple halos during plaque formation further enhances research value. complete sequence has been submitted GenBank under accession number PP663283.

Language: Английский

Citations

0
Beyond antibiotics: exploring multifaceted approaches to combat bacterial resistance in the modern era: a comprehensive review DOI Creative Commons
Aref Yarahmadi,

Hamide Najafiyan,

Mohammad Hasan Yousefi

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 18, 2025

Antibiotics represent one of the most significant medical breakthroughs twentieth century, playing a critical role in combating bacterial infections. However, rapid emergence antibiotic resistance has become major global health crisis, significantly complicating treatment protocols. This paper provides narrative review current state resistance, synthesizing findings from primary research and comprehensive articles to examine various mechanisms bacteria employ counteract antibiotics. One sources is improper use antibiotics livestock industry. The drug-resistant microorganisms human activities industrial production presented environmental public concerns. Today, resistant nosocomial infections occur following long-term hospitalization patients, causing death many people, so there an urgent need for alternative treatments. In response this non-antibiotic therapeutic strategies have been proposed, including bacteriophages, probiotics, postbiotics, synbiotics, fecal microbiota transplantation (FMT), nanoparticles (NPs), antimicrobial peptides (AMPs), antibodies, traditional medicines, toxin-antitoxin (TA) system. While these approaches offer innovative solutions addressing preserving efficacy therapies, challenges such as safety, cost-effectiveness, regulatory hurdles, large-scale implementation remain. examines potential limitations strategies, offering balanced perspective on their managing mitigating broader impact resistance.

Language: Английский

Citations

0
A One Health Approach Addressing Poultry-Associated Antimicrobial Resistance: Human, Animal and Environmental Perspectives DOI Creative Commons
Samradhi Singh, Mona Kriti,

Aguduri Lakshmi S.

et al.

The Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 100309 - 100309

Published: March 1, 2025

Language: Английский

Citations

0
Deciphering the Gut–Liver Axis: A Comprehensive Scientific Review of Non-Alcoholic Fatty Liver Disease DOI Creative Commons
Samradhi Singh, Mona Kriti, Roberto Catanzaro

et al.

Livers, Journal Year: 2024, Volume and Issue: 4(3), P. 435 - 454

Published: Sept. 12, 2024

Non-alcoholic fatty liver disease (NAFLD) has emerged as a significant global health issue. The condition is closely linked to metabolic dysfunctions such obesity and type 2 diabetes. gut–liver axis, bidirectional communication pathway between the gut, plays crucial role in pathogenesis of NAFLD. This review delves into mechanisms underlying exploring influence gut microbiota, intestinal permeability, inflammatory pathways. also explores potential therapeutic strategies centered on modulating microbiota fecal transplantation; phage therapy; use specific probiotics, prebiotics, postbiotics managing By understanding these interactions, we can better comprehend development advancement NAFLD identify targets.

Language: Английский

Citations

0
Therapeutic and Diagnostic Potential of a Novel K1 Capsule Dependent Phage, JSSK01, and Its Depolymerase in Multidrug-Resistant Escherichia coli Infections DOI Open Access

Naveen Gattuboyena,

Yu-Chuan Tsai,

Ling‐Chun Lin

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12497 - 12497

Published: Nov. 21, 2024

Bacteriophages are viruses that have the potential to combat bacterial infections caused by antimicrobial-resistant strains. In this study, we investigated a novel lytic bacteriophage, vB_EcoS_JSSK01, isolated from sewage in Hualien, Taiwan, which effectively combats multidrug-resistant (MDR)

Language: Английский

Citations

0
Isolation and Characterization of Lytic Bacteriophages Against Multi Drug-Resistant Acinetobacter Baumannii DOI

Namrata Pal,

Poonam Sharma, Samradhi Singh

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Standardization of the Agar Plate Method for Bacteriophage Production DOI Creative Commons
Su Jin Jo, Young Min Lee,

K. K. Cho

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 14(1), P. 2 - 2

Published: Dec. 24, 2024

The growing threat of antimicrobial resistance (AMR), exacerbated by the COVID-19 pandemic, highlights urgent need for alternative treatments such as bacteriophage (phage) therapy. Phage therapy offers a targeted approach to combat bacterial infections, particularly those resistant conventional antibiotics. This study aimed standardize an agar plate method high-mix, low-volume phage production, suitable personalized Plaque assays were conducted with double-layer method, and plaque sizes precisely measured using image analysis tools. Regression models developed Minitab software established correlations between size optimizing production while minimizing development. resulting Size Calculation (PSC) model accurately correlated inoculum concentration yield, establishing specific plaque-forming unit (PFU) thresholds optimal production. Using phages targeting pathogens Escherichia, Salmonella, Staphylococcus, Pseudomonas, Chryseobacterium, Vibrio, Erwinia, Aeromonas confirmed model's accuracy across various conditions. validation showed strong inverse correlation minimum-lawn cell clearing PFUs (MCPs; R² = 98.91%) identified density that maximizes yield evolution mutants. These results highlight PSC standardized scalable efficient which is crucial AMR management. Furthermore, its adaptability different conditions positions it potential standard tool rapid precise screening propagation in both clinical industrial settings.

Language: Английский

Citations

0