Journey of the Probiotic Bacteria: Survival of the Fittest

Microorganisms, Journal Year: 2022, Volume and Issue: 11(1), P. 95 - 95

Published: Dec. 30, 2022

This review aims to bring a more general view of the technological and biological challenges regarding production use probiotic bacteria in promoting human health. After brief description current concepts, for at an industrial level are presented from physiology central metabolism ability face main forms stress process. Once produced, these cells processed be commercialized suspension or dried added food matrices. At this stage, maintenance cell viability vitality is paramount quality product. Powder products requires development strategies that ensure integrity components cellular functions allow complete recovery time consumption. Finally, once consumed, must very powerful set physicochemical mechanisms within body, which include enzymes, antibacterial molecules sudden changes pH. Understanding action agents induction tolerance fundamental selection increasingly efficient strains order survive colonization intestinal tract promote desired health benefits.

Language: Английский

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Molecular Targets in Campylobacter Infections

Biomolecules, Journal Year: 2023, Volume and Issue: 13(3), P. 409 - 409

Published: Feb. 22, 2023

Human campylobacteriosis results from foodborne infections with Campylobacter bacteria such as jejuni and coli, represents a leading cause of bacterial gastroenteritis worldwide. After consumption contaminated poultry meat, constituting the major source pathogenic transfer to humans, infected patients develop abdominal pain diarrhea. Post-infectious disorders following acute enteritis may occur affect nervous system, joints or intestines. Immunocompromising comorbidities in favor bacteremia, vascular inflammation septicemia. Prevention human infection is achieved by hygiene measures focusing on reduction food contamination. Molecular targets for treatment prevention include pathogenicity virulence factors involved motility, adhesion, invasion, oxygen detoxification, acid resistance biofilm formation. This repertoire intervention has recently been completed drugs dampening pro-inflammatory immune responses induced endotoxin lipo-oligosaccharide. Novel pharmaceutical strategies will combine anti-pathogenic anti-inflammatory effects reduce risk both anti-microbial post-infectious sequelae enteritis. actual trends combat are presented this review, alongside molecular applied strategies.

Language: Английский

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Assessment of probiotic Bacillus velezensis supplementation to reduce Campylobacter jejuni colonization in chickens

Poultry Science, Journal Year: 2024, Volume and Issue: 103(8), P. 103897 - 103897

Published: May 23, 2024

Campylobacter jejuni continues to be a major public health issue worldwide. Poultry are recognized as the main reservoir for this foodborne pathogen. Implementing measures decrease C. colonization on farms has been regarded most effective strategy control incidence of campylobacteriosis. The probiotics supplementation an attractive approach against in chickens. Here inhibitory effects one probiotic B. velezensis isolate CAU277 was evaluated vitro and vivo. antimicrobial activity showed that supernatant exhibited pronounced strains compared other bacterial species. When co-cultured with velezensis, growth reduced significantly from 7.46 log10 CFU/mL (24 h) 1.02 (48 h). Further, remained stable under broad range temperature, pH, protease treatments. vivo experiments demonstrated oral administration by 2.0 CFU/g feces chicken cecum at 15 days post-inoculation. In addition, supplementary increased microbial species richness diversity ileum, especially enhanced population Alistipes Christensenellaceae, decreased existence Lachnoclostridium. Our study presents possesses activities promotes microbiota intestines. These findings indicate potential develop additive infection chicken.

Language: Английский

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Prospective Application of Nanoencapsulated Bacillus amyloliquefaciens on Broiler Chickens’ Performance and Gut Health with Efficacy against Campylobacter jejuni Colonization

Animals, Journal Year: 2023, Volume and Issue: 13(5), P. 775 - 775

Published: Feb. 21, 2023

Probiotics as novel antibiotics’ substitutes are verified to provide barriers for hindering the colonization of enteric bacterial pathogens with nutritional benefits. For enhancement probiotics’ effectiveness, their integration within nanomaterials is a paramount tool support progress new compounds functional features. Therefore, we addressed impact effective delivery probiotics (Bacillus amyloliquefaciens) loaded nanoparticles (BNPs) on performance and Campylobacter jejuni (C. jejuni) shedding in poultry. Two hundred Ross broiler chickens were divided into four groups fed various BNP levels: BNPs I, II, III, BNPs-free diets 35 days. Nanoparticles improved growth reflected by higher body weight gain superior feed conversion ratio, especially II- III-fed groups. In parallel, mRNA expression levels digestive enzymes encoding genes (AMY2a, PNLIP, CELA1, CCK) achieved peaks group (1.69, 1.49, 1.33, 1.29-fold change, respectively) versus control one. Notably, increasing BNPs, abundance beneficial microbiota, such Bifidobacterium Lactobacillus species, was favored over harmful ones, including Clostridium species Enterobacteriaceae. Birds displayed significant improvement barrier functions-linked DEFB1, FABP-2, MUC-2 alongside substantial reduction cecal fecal C. jejuni. From aforementioned positive effects concluded potential roles promoters preventive aids infection

Language: Английский

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Therapeutic and protective approaches to combat Campylobacter jejuni infections

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: May 6, 2025

Campylobacter jejuni is a typical zoonotic bacterium, colonizing the gut of many bird species as commensal. In humans, C. major foodborne pathogen. Infection humans causes campylobacteriosis in small intestine, constituting main source bacteria-dependent gastroenteritis cases worldwide. particular, ingestion under-cooked rooster meat, raw milk and contaminated water, well cross-contamination ready-to-eat food after handling chicken are responsible for majority infections. As consequence, infected individuals may acquire watery and/or bloody diarrhea associated with abdominal pain, eventually post-infection illnesses neural system joints, including Guillain-Barré, Miller Fisher Reiter syndromes. One therapeutic strategy to reduce colonization farms using vaccination, bacteriocins phage therapy protocols. Prevention approaches during poultry meat processing comprise compliance high hygiene standards. Furthermore, substantial progress has been also made recent years combat established mouse vitro cell model systems. this regard, specific colonization- pathogenicity-associated components were considered favored treatment structures, targeting bacterial movement, host interaction, intracellular survival, propagation spread bacteria. This complemented by number pharmaceutical compounds jejuni-induced epithelial damage, inflammation apoptosis mice. Here we review these novel prevention "One World - Health" that aim diminish consequences acute sequelae humans.

Language: Английский

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Exploring the Contribution of Campylobacter jejuni to Post-Infectious Irritable Bowel Syndrome: A Literature Review

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(8), P. 3373 - 3373

Published: April 17, 2024

This comprehensive review investigates the specific impact of foodborne pathogen Campylobacter jejuni (C. jejuni) on gastrointestinal health, focusing its connection to post-infectious irritable bowel syndrome (PI-IBS). examines pathogen’s pathophysiology, clinical implications and epidemiological trends using recent research data highlight prevalence association with PI-IBS. A detailed literature analysis synthesizes current illuminate Campylobacter’s long-lasting effects gut microbiota intestinal function. It provides a shed light C. jejuni’s long-term The findings suggest need for multifaceted prevention treatment approaches considering individual, microbial factors, thus contributing more nuanced understanding PI-IBS following infection.

Language: Английский

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Isolation of Bacillus licheniformis and its protective effect on liver oxidative stress and apoptosis induced by aflatoxin B1

Poultry Science, Journal Year: 2024, Volume and Issue: 103(10), P. 104079 - 104079

Published: July 10, 2024

Aflatoxin B1 (AFB1) is one of the most toxic mycotoxins. The use probiotics an effective approach to reduce aflatoxins content in foods. To find efficient bacterial species that can eliminate or detoxify AFB1, a strain S51 capable degrading AFB1 was isolated from chicken intestine and soil samples by using culture medium containing coumarin as sole carbon source. Based on results 16S rRNA gene sequence analysis, this isolate (strain S51) identified Bacillus licheniformis QT338. Further characterization showed it could degrade 61.3% after incubation at 30°C for 72 h. Additional studies demonstrated promoted good growth performance treated chickens, no hemolytic activity, carried few drug resistance genes, exhibited certain level tolerance acid bile salts. Furthermore, verify whether exerts protective effect AFB1-induced liver injury chickens elucidate underlying mechanism, toxicity model induced with (100 μg/kg BW) S51(1×10

Language: Английский

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Deciphering the Association between Campylobacter Colonization and Microbiota Composition in the Intestine of Commercial Broilers

Microorganisms, Journal Year: 2023, Volume and Issue: 11(7), P. 1724 - 1724

Published: June 30, 2023

is a major food safety concern and transmitted mainly via poultry meat. We previously found that some commercial broiler farms consistently produced

Language: Английский

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Gut microbiota profiling in aged dogs after feeding pet food contained Hericium erinaceus

Journal of Animal Science and Technology, Journal Year: 2022, Volume and Issue: 64(5), P. 937 - 949

Published: Sept. 1, 2022

Health concern of dogs is the most important issue for pet owners. People who have companied long-term provide utmost cares their well-being and healthy life. Recently, it was revealed that population types gut microbiota affect metabolism immunity host. However, there little information on microbiome dogs. Hericium erinaceus (H. erinaceus; HE) one well-known medicinal mushrooms has multiple bioactive components including polyphenol, β-glucan, polysaccharides, ergothioneine, hericerin, erinacines, etc. Here we tested a food contained H. improvement in environment aged A total 18 dogs, each 11 years old, were utilized. For sixteen weeks, fed with 0.4 g (HE-L), or 0.8 (HE-H), without (CON) per body weight (kg) daily diets (n = 6 group). Taxonomic analysis performed using metagenomics to investigate difference microbiome. Resulting from principal coordinates (PCoA) confirm distance between groups, significant HE-H CON due weighted Unique fraction metric (Unifrac) (p 0.047), but HE-L did not statistical compared CON. Additionally, result Linear discriminate effect size (LEfSe) showed phylum Bacteroidetes its order Bacteroidales increased, CON, Firmicutes HE-H, genera (Streptococcus, Tyzzerella) reduced. Furthermore, at family level, Campylobacteraceae genus Campylobacter decreased Summarily, our data demonstrated intake can regulate microbial community an adequate supply HE would possibly improve anti-obesity gut-microbiota

Language: Английский

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Predicting foodborne pathogens and probiotics taxa within poultry-related microbiomes using a machine learning approach

Animal Microbiome, Journal Year: 2023, Volume and Issue: 5(1)

Published: Nov. 15, 2023

Microbiomes that can serve as an indicator of gut, intestinal, and general health humans animals are largely influenced by food consumed contaminant bioagents. Microbiome studies usually focus on estimating the alpha (within sample) beta (similarity/dissimilarity among samples) diversities. This study took a combinatorial approach applied machine learning to microbiome data predict presence disease-causing pathogens their association with known/potential probiotic taxa. Probiotics beneficial living microorganisms capable improving host organism's digestive system, immune function ultimately overall health. Here, 16 S rRNA gene high-throughput Illumina sequencing temporal pre-harvest (feces, soil) samples 42 pastured poultry flocks (poultry in this entire work solely refers chickens) from southeastern U.S. farms was used generate relative abundance operational taxonomic units (OTUs) input. Unique genera OTUs were predictors prevalence foodborne (Salmonella, Campylobacter Listeria) at different stages growth (START (2-4 weeks old), MID (5-7 END (8-11 old)), farm management practices physicochemical properties.While we did not see any significant associations between known probiotics Salmonella or Listeria, observed negative correlations (Bacillus Clostridium) mid-time point sample collection. Our indicates correlation potential both early end-time points Furthermore, our model prediction shows changes operations such how often houses moved pasture, age which chickens introduced diet composition other could favorably increase activity reduce prevalence.Integration using provided insights transmission along farm-to-fork continuum. Altering support proliferation pathogen identified here constitute complementary method existing but ineffective interventions vaccination bacteriophage cocktails usage. Study findings also corroborate bacterial Caloramator, DA101, Parabacteroides Faecalibacterium probiotics.

Language: Английский

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