Dmrt1 is necessary for male sexual development in zebrafish

Developmental Biology, Journal Year: 2016, Volume and Issue: 422(1), P. 33 - 46

Published: Dec. 8, 2016

Language: Английский

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Dosage Compensation of the X Chromosome: A Complex Epigenetic Assignment Involving Chromatin Regulators and Long Noncoding RNAs

Annual Review of Biochemistry, Journal Year: 2018, Volume and Issue: 87(1), P. 323 - 350

Published: April 18, 2018

X chromosome regulation represents a prime example of an epigenetic phenomenon where coordinated whole is required. In flies, this achieved by transcriptional upregulation chromosomal genes in males to equalize the gene dosage differences females. Chromatin-bound proteins and long noncoding RNAs (lncRNAs) constituting ribonucleoprotein complex known as male-specific lethal (MSL) or compensation mediate process. MSL members decorate male chromosome, their absence leads lethality. The also enriched with histone H4 lysine 16 acetylation (H4K16ac), indicating that chromatin compaction status plays important role activation. How specifically targeted how mechanistically are central questions for field. Here, we review recent advances, which reveal interplay among lncRNAs, landscape, transcription, conformation fine-tune expression.

Language: Английский

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Engineering the Composition and Fate of Wild Populations with Gene Drive

Annual Review of Entomology, Journal Year: 2020, Volume and Issue: 66(1), P. 407 - 434

Published: Oct. 9, 2020

Insects play important roles as predators, prey, pollinators, recyclers, hosts, parasitoids, and sources of economically products. They can also destroy crops; wound animals; serve vectors for plant, animal, human diseases. Gene drive-a process by which genes, gene complexes, or chromosomes encoding specific traits are made to spread through wild populations, even if these result in a fitness cost carriers-provides new opportunities altering populations benefit humanity the environment ways that species sustainable. drive be used alter genetic composition an existing population, referred population modification replacement, bring about suppression elimination. We describe technologies under consideration, progress has been made, remaining technological hurdles, particularly with respect evolutionary stability our ability control ultimate fate genes introduced into populations.

Language: Английский

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A Wolbachia factor for male killing in lepidopteran insects

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Nov. 14, 2022

Bacterial symbionts, such as Wolbachia species, can manipulate the sexual development and reproduction of their insect hosts. For example, infection induces male-specific death in Asian corn borer Ostrinia furnacalis by targeting host factor Masculinizer (Masc), an essential protein for masculinization dosage compensation lepidopteran insects. Here we identify a protein, designated Oscar, which interacts with Masc via its ankyrin repeats. Embryonic expression Oscar inhibits Masc-induced leads to male killing two insects, O. silkworm Bombyx mori. Our study identifies mechanism induce progeny.

Language: Английский

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Asymmetric interactions between doublesex and tissue- and sex-specific target genes mediate sexual dimorphism in beetles

Nature Communications, Journal Year: 2017, Volume and Issue: 8(1)

Published: Feb. 27, 2017

Abstract Sexual dimorphisms fuel significant intraspecific variation and evolutionary diversification. Yet the developmental-genetic mechanisms underlying sex-specific development remain poorly understood. Here, we focus on conserved sex-determination gene doublesex ( dsx ) by which it mediates in a horned beetle species combining systemic knockdown, high-throughput sequencing of diverse tissues genome-wide analysis Dsx-binding sites. We find that Dsx regulates sex-biased expression predominantly males, Dsx's target repertoires are highly sex- tissue-specific can exercise its regulatory role via two distinct mechanisms: as modulator regulating strictly targets, or switch same genes males females opposite directions. More generally, our results suggest rapidly acquire new to accommodate evolutionarily novel traits, evidenced large unique repertoire identified head horns, recent morphological innovation.

Language: Английский

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Sex Sorting for Pest Control: It’s Raining Men!

Trends in Parasitology, Journal Year: 2019, Volume and Issue: 35(8), P. 649 - 662

Published: June 26, 2019

Sexing Diptera represents a major obstacle to operationalizing vector-control methods based on the mass release of males, such as sterile insect technique or incompatible technique.The recent progress made in gene editing, well growing understanding sex-determination pathways Diptera, offer new perspectives develop sexing systems target species that allow female elimination during rearing.The developmental stage at which sexes are separated, females eliminated, is one key parameters for assessing cost efficiency technology.Challenges remain development sex-separating used early rearing, without genetic defects lack competitiveness produced good social and regulatory acceptability used, considering their operational deployment field. In pursuit better pest- strategies, attention returns an old proven technology, (SIT) related population-suppression methods. A any these approaches involves males separation from rearing stage, order improve cost-efficiency prevent biting disease-vectoring females. This review describes sex-sorting developments dipteran flies with emphasis suitability large-scale male vectors release. Disease-vectoring responsible millions parasitic viral infections humans livestock annually [1.Kassebaum N.J. et al.Global, regional, national disability-adjusted life-years (DALYs) 315 diseases injuries healthy life expectancy (HALE), 1990–2015: systematic analysis Global Burden Disease Study 2015.Lancet. 2016; 388: 1603-1658Abstract Full Text PDF PubMed Scopus (1342) Google Scholar]. As more environmentally friendly alternative broad-spectrum chemical insecticides, many researchers have been inspired by Knipling's proposal 1955 [2.Knipling E.F. Possibilities control eradication through use sexually males.J. Econ. Entomol. 1955; 48: 459-462Crossref Scholar] releasing reduce pest populations. so-called (see Glossary) has effective variety insects, but its implementation slowed down necessity removing before case mosquitoes. addition minimizing health economic risks posed released females, models trials also shown only was much cost-efficient than both Scholar, 3.Rendón P. al.Medfly (Diptera:Tephritidae) sexing: field comparison males-only bisexual fly releases Guatemala.J. 2004; 97: 1547-1553Crossref (198) These savings may arise either reduced mass-rearing insects and/or performance, where will not be distracted coreleased Other methods, including carrying dominant lethal (RIDL) Wolbachia-based (IIT), require consistent Models show small proportion Wolbachia-infected could lead population replacement instead eliminationi. mosquitoes, whose cause nuisance transmit pathogens, very little contamination can tolerated strategy. applications, sex sorting, 'sexing', refers specifically removal rely mechanical natural engineered dimorphic differences, complex technologies modify expression conditionally masculinize kill development. Overall, sex-separation strategies need meet several criteria, summarized 'the 7 Ses' Papathanos colleagues [4.Papathanos P.A. al.Sex strategies: past experience approaches.Malar. J. 2009; 8S5Crossref (94) Scholar]: small, simple, switchable, stable, stringent, sexy, sellable. reviewed numerous times [5.Alphey L. Genetic mosquitoes.Annu. Rev. 2014; 59: 205-224Crossref (254) 6.Gilles J.R.L. al.Towards mosquito programmes: Exploring genetic, molecular, behavioural mosquitoes.Acta Trop. 132: S178-S187Crossref (72) 7.Bernardini F. al.Molecular tools markers generation transgenic strains Anopheline mosquitoes.Parasit. Vectors. 2018; 11: 660Crossref (7) 8.Häcker I. Schetelig M.F. Molecular create vector control.Parasit. 645Crossref (4) Scholar], focusing mostly particular genera, engineering employed. Here, we aim all developed recently Diptera. We chosen focus solely since there number interesting technical other complement mosquitoes disease vectors. With perspective explored advantages disadvantages each innovation context compared what currently being done. Our survey starts 2003, most comprehensive available published 2002 [9.Robinson A.S. Mutations control.Mutat. Res. Mutat. 2002; 511: 113-132Crossref (74) Most current-day programs always option. For instance, Australian Thai Bactrocera facilities produce tens fruit per week [10.Fanson B.G. al.A 16 years quality facility producing Queensland fly, tryoni.Entomol. Exp. Appl. 151: 152-159Crossref (53) 11.Orankanok W. al.Area-wide integrated oriental dorsalis guava correcta Thailand.in: Vreysen M.J.B. Area-Wide Control Insect Pests. Springer, 2007: 517-526Crossref (52) Similarly, screwworm Cochliomyia hominivorax targeted weekly 15 million Panama–Colombia border (reviewed [12.Scott M.J. al.Review research advances program over 25 years.Entomol. 2017; 164: 226-236Crossref (38) Scholar]), this scale production, method pupal considered [13.Concha C. male-only strain New World improved using technique.BMC Biol. 14: 72Crossref (47) predicted US$ 1 year. To authors' knowledge, Mediterranean Ceratitis capitata Mexican Anastrepha ludens amongst agricultural importance plants around world, improving greatly efficiencyii [14.Augustinos A.A. al.Ceratitis strains: laboratory evaluation worldwide.Entomol. 305-317Crossref (29) Where mandatory, time-consuming often Culicinae (including genera Aedes Culex) exhibit size sexual dimorphism pupae. Consequently, been, still, mechanically separated [15.Papathanos current status efficient 654Crossref (27) Italy, SIT trial Ae. albopictus initiated 2005 [16.Bellini R. al.Pilot irradiated Italian urban areas.J. Med. 2013; 50: 317-325Crossref (107) Over 4-year period, 2 were sex-sorted metal sieving plates recovered 26–29% still about 1.2%. China, Fay-Morlan glass sorters, size, enabled 197 2016 2017 combined IIT/SIT two river island settings, greater recovery, 0.3% [17.Zheng X. al.Incompatible techniques eliminate mosquitoes..Nature. 2019; https://doi.org/10.1038/s41586-019-1407-9Crossref (236) Similar dimorphism-based deployed Dengue La Reunion Island against albopictusiii French Polynesia aegypti polynesiensisiv,v. Anopheles manual identification, allows 500 pupae hour Scholar]). The working time therefore high, necessary difficult reach. tsetse flies, feed exclusively blood act trypanosomes. Release trypanocide-treated demands low-throughput chilled adults protogyny [18.Bouyer al.Ex-ante benefit-cost Glossina palpalis gambiensis Niayes Senegal.PLoS Negl. Dis. 8e3112Crossref (31) 19.Seck M.T. al.Quality after long distance transport chilled, pupae.PLoS 2015; 9e0004229Crossref (32) During Senegal, 5 protogyny-based sorting [19.Seck Sex-sorting adult requires feeding larvae, latter discarded retain males. Moreover, increasing densities larvae would fitness slow [20.Agnew minimalist approach effects density-dependent competition life-history traits.Ecol. 27: 396-402Crossref (104) Therefore, advantageous avoid between [21.Phuc H.K. al.Late-acting control.BMC 2007; 5: 11Crossref (308) When translates into time, labor, money decreases workers facility. reasons, distinguishing categories: first larval stages later Disruption pathway goal identifying genes essential Drosophila melanogaster system template inquiry cascades. However, even within closely species, exploration primary signal downstream factors revealed differences. Box reviews our signals manipulated male-biased populations insect's (Table 1). Although few them actually applied conditions, classified innovations according outcome, indicator potential efficiency.Box 1The Sex Determination Pathways DipteraSexual controlled diverse mechanisms determine differentiate morphologies. Figure I illustrates known pathways.Sex-determination range familiar many: dosage compensation X Y chromosomes [83.Lucchesi J.C. Kuroda M.I. Dosage Drosophila.Cold Spring Harb. Perspect. 7a019398Crossref (114) homomorphic aegypti, transcription autosomal nix appears maleness (M) factor initiating determination [84.Hall A.B. male-determining aegypti.Science. 348: 1268-1270Crossref (171) Rapid evolution high degree variation function involved, marked differences described [85.Sharma A. al.Male houseflies determined Mdmd, paralog generic splice CWC22.Science. 356: 642-645Crossref (69) Scholar].What common studied date central regulator doublesex (dsx), female-specific exons transcripts induce sterility lethality [39.Whyard S. al.Silencing buzz: suppression double-stranded RNAs.Parasit. 8: 96Crossref 86.Kyrou K. CRISPR–Cas9 drive targeting causes complete caged gambiae mosquitoes.Nat. Biotechnol. 36: 1062-1066Crossref (350) 87.Clough E. al.Sex- tissue-specific functions genes.Dev. Cell. 31: 761-773Abstract (73) Dsx doublesex/mab-3 family. Recently Kopp [88.Kopp Dmrt dimorphism.Trends Genet. 2012; 28: 175-184Abstract (188) family conserved arthropods, diversity roles dsx plays lineages [89.Verhulst E.C. van de Zande Double nexus – connecting element determination.Brief. Funct. Genomics. 396-406Crossref (46) suggests it frequently coopted roles. Briefly, expressed tissues identity. melanogaster, regulated splicing transformer (tra), another successfully lethality, which, turn, sex-lethal (sxl) muscids date, tra upstream so far, determining (Aedes aegypti) [90.Gomulski L.M. al.The Nix locus male-specific homologue chromosome strong candidate factor.Parasit. 647Crossref (12) yob (An. gambiae) [40.Krzywinska malaria gambiae.Science. 353: 67-69Crossref (70) guy1 stephensi) [91.Criscione al.GUY1 confers stephensi.eLife. 5e19281Crossref presumed regulators dsx, regulating dsx. role tra-2 sperm shown, resulting reduction offspring second RNAi knockdown [38.Hoang K.P. al.Mechanisms transmission ratio distortion aegypti.Parasit. 9: 49Crossref (16) Intriguingly, putative tra/tra2 orthologues appear highly implicated mosquito, although involved ovarian [92.Li al.Two three Transformer-2 required albopictus.Insect Biochem. Mol. 109: 92-105Crossref (9) M-factor tephritid regulate tra's auto-regulatory positive-feedback loop [93.Meccariello al.Maleness-on-the-Y (MoY) orchestrates pests.bioRxiv. (Published online February 7, 2019. http://doi.org/10.1101/533646)Google phlebotomine sandflies, identified self-regulating [94.Petrella V. al.Genomics transcriptomics unravel sand flies.bioRxiv. January 4, https://doi.org/10.1101/510586)Google There likely opportunities distort ratios discovering players evolved Diptera.Table 1Early Acting Methods 2003OutcomeTechniqueaTechniques detailed S1 supplemental information online.Sorting mechanismSpeciesRefsSex distortionInterfering RNASilencing (tra) transformer-2 (tra-2)Ceratitis capitata22.Saccone G. al.New capitata: transforming males.in: 95-102Crossref (25) ScholarVisual separationTransposase-mediated plasmid integrationRFP GFP marker + COPASAnopheles gambiae, arabiensis28.Marois al.High-throughput studies future interventions.Malar. 302Crossref 29.Bernardini al.Site-specific chromosome.Proc. Natl. Acad. Sci. U. 111: 7600-7605Crossref (49) 30.Bernardini al.Cross-species complex.Genetics. 207: 729-740PubMed ScholartTA intron fluorescent markerLucilia cuprina31.Li al.Transgenic sheep blow Lucilia cuprina.Insect 51: 80-88Crossref (44) ScholarFemale lethalityTransposase-mediated integration (piggyBac)tTA driving proapoptotic transgeneAnastrepha suspensa, capitata, cuprina32.Ogaugwu C.E. (Diptera: Tephritidae) embryonic lethality.Insect 43: 1-8Crossref 33.Schetelig Handler A.M. suspensa Tephritidae).Insect 42: 790-795Crossref (75) 34.Yan Y. al.Building early-larval cuprina constitutive promoters.Sci. Rep. 7: 2538Crossref (17) ScholarInterfering tra-2Bactrocera dorsalis, aegypti37.Liu al.RNAi-mediated knock-down generate progeny (Hendel).PLoS One. 10e0128892PubMed 38.Hoang ScholarSilencing dsxAe. aegypti39.Whyard ScholarmRNA injectionOverexpression Yob injecting mRNAAn. arabiensis40.Krzywinska ScholarIntersex femalesTransposase-mediated (piggyBac)Plasmid injection overexpression under vasa2 promoterAn. gambiae41.Krzywinska Krzywinski Effects stable ectopic gambiae.Parasit. 648Crossref (10) ScholarCRISPR-Cas9 knockdownDouble tra-2A. suspensa42.Li CRISPR/Cas9-mediated editing exogenous transgene endogenous Caribbean suspensa.Gene. 691: 160-166Crossref (8) Scholara Techniques online. Open table tab Sexual pathways. Sex-determination What th

Language: Английский

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A sex-specific switch between visual and olfactory inputs underlies adaptive sex differences in behavior

Current Biology, Journal Year: 2021, Volume and Issue: 31(6), P. 1175 - 1191.e6

Published: Jan. 27, 2021

Although males and females largely share the same genome nervous system, they differ profoundly in reproductive investments require distinct behavioral, morphological, physiological adaptations. How can while bound by both developmental biophysical constraints, produce these sex differences behavior? Here, we uncover a novel dimorphism Drosophila melanogaster that allows deployment of completely different behavioral repertoires with minimum changes to circuit architecture. Sexual differentiation only small number higher order neurons brain leads change connectivity related primary needs sexes—courtship pursuit communal oviposition females. This study explains how an apparently similar generates two sexes presents fundamental principle neural organization may be extended other species.

Language: Английский

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Population suppression by release of insects carrying a dominant sterile homing gene drive targeting doublesex in Drosophila

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 14, 2024

Language: Английский

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Wolbachia symbionts control sex in a parasitoid wasp using a horizontally acquired gene

Current Biology, Journal Year: 2024, Volume and Issue: 34(11), P. 2359 - 2372.e9

Published: April 30, 2024

Host reproduction can be manipulated by bacterial symbionts in various ways. Parthenogenesis induction is the most effective type of manipulation for their transmission. Insect sex determined regulation doublesex (dsx) splicing through transformer2 (tra2) and transformer (tra) interaction. Although parthenogenesis has been studied since 1970s, its underlying molecular mechanism unknown. Here we identify a Wolbachia parthenogenesis-induction feminization factor gene (piff) that targets sex-determining genes causes female-producing haplodiploid parasitoid Encarsia formosa. We found elimination repressed expression female-specific dsx enhanced male-specific dsx, which led to production wasp haploid male offspring. Furthermore, E. formosa tra truncated non-functional, functional homolog, termed piff, with an insect origin. PIFF colocalize interact TRA2. Moreover, piff coordinated tra2 Our results demonstrate symbiont acquired manipulate host determination cascade induce wasps. This study reveals insect-to-bacteria horizontal transfer drives evolution animal systems, elucidating striking insect-microbe symbiosis.

Language: Английский

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Zygosity-based sex determination in a butterfly drives hypervariability of Masculinizer

Science Advances, Journal Year: 2024, Volume and Issue: 10(18)

Published: May 3, 2024

Nature has devised many ways of producing males and females. Here, we report on a previously undescribed mechanism for Lepidoptera that functions without female-specific gene. The number alleles or allele heterozygosity in single Z-linked gene ( BaMasc ) is the primary sex-determining switch Bicyclus anynana butterflies. Embryos carrying develop into WZ (or Z0) females, those two distinct ZZ males, while (ZZ) homozygotes initiate female development, have mismatched dosage compensation, die as embryos. Consequently, selection against favored evolution spectacular allelic diversity: 205 different coding sequences were detected sample 246 structural similarity hypervariable region (HVR) to HVR Apis mellifera csd suggests molecular convergence between deeply diverged insect lineages. Our discovery this highlights fascinating diversity mechanisms underlying evolutionary drivers.

Language: Английский

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