The application value of bacteriophage in patients with severe drug-resistant bacterial infections DOI
Juan Chen, Man Huang

Journal of Translational Critical Care Medicine, Journal Year: 2024, Volume and Issue: 6(4)

Published: Sept. 19, 2024

The emergence of antimicrobial resistance (AMR) represents one the most significant global public health threats, imposing substantial economic burdens. To address this critical issue resistance, promising alternative strategies for treating resistant bacterial infections is bacteriophage therapy. Bacteriophages exhibit high specificity towards bacteria, capable lysing and killing specific strains. Phage cocktails can broaden spectrum activity, contribute to target multiple types bacteria. Personalized therapy, tailored individual patients, enhances precise targeting pathogens. Increasing evidence supports feasibility phage-antibiotic combinations in multidrug-resistant Furthermore, genetic engineering, including technologies like clustered regularly interspaced short palindromic repeats (CRISPR)-Cas, has advanced development engineering phages, providing novel ways kill hosts. This review discusses research progress refractory caused by advantages limitations phage highlights clinical studies case reports various treatment modalities.

Language: Английский

Phage therapy DOI
Mikael Skurnik,

Sivan Alkalay‐Oren,

Maarten Boon

et al.

Nature Reviews Methods Primers, Journal Year: 2025, Volume and Issue: 5(1)

Published: Feb. 13, 2025

Citations

2
Phage therapy: an alternative treatment modality for MDR bacterial infections DOI

Namrata Pal,

Poonam Sharma, Manoj Kumawat

et al.

Infectious Diseases, Journal Year: 2024, Volume and Issue: 56(10), P. 785 - 817

Published: July 17, 2024

The increasing global incidence of multidrug-resistant (MDR) bacterial infections threatens public health and compromises various aspects modern medicine. Recognising the urgency this issue, World Health Organisation has prioritised development novel antimicrobials to combat ESKAPEE pathogens. Comprising

Language: Английский

Citations

10
Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches DOI Creative Commons
Minyoung Kevin Kim, Gina A. Suh, Grace Cullen

et al.

Journal of Clinical Investigation, Journal Year: 2025, Volume and Issue: 135(5)

Published: March 2, 2025

Bacteriophage (phage) therapy has emerged as a promising solution to combat the growing crisis of multidrug-resistant (MDR) infections. There are several international centers actively engaged in implementation phage therapy, and recent case series have reported encouraging success rates patients receiving personalized, compassionate for difficult-to-treat Nonetheless, substantial hurdles remain way more widespread adoption consistent success. This Review offers comprehensive overview current technologies therapeutic approaches. We first delineate common steps development, from bank establishment clinical administration, examine spectrum approaches, personalized fixed cocktails. Using framework conventional drug development pipeline, we then identify critical knowledge gaps areas such cocktail design, formulation, pharmacology, trial design. conclude that, while holds promise, structured pipeline sustained government support crucial MDR

Language: Английский

Citations

1
Successful phage-antibiotic therapy of P. aeruginosa implant-associated infection in a Siamese cat DOI Creative Commons

Ron Braunstein,

Goran Hubanic,

Ortal Yerushalmy

et al.

Veterinary Quarterly, Journal Year: 2024, Volume and Issue: 44(1), P. 1 - 9

Published: May 10, 2024

Antibiotic-resistant pathogens are a growing global issue, leading to untreatable infectious diseases in both humans and animals. Personalized bacteriophage (phage) therapy, the use of specific anti-bacterial viruses, is currently approach combat antibiotic-resistant infections. The implementation phage therapy has primarily been focused on humans, almost neglecting impact such infections health welfare companion Pets also have potential spread resistant their owners or veterinary staff through zoonotic transmission. Here, we showcase personalized phage-antibiotic treatment cat with multidrug-resistant Pseudomonas aeruginosa implant-associated infection post-arthrodesis surgery. encompassed tailored combination an anti-P. ceftazidime, precisely matched pathogen. was topically applied surgical wound while antibiotic administered intramuscularly. After two courses spanning 7 3 weeks, wound, which had previously remained open for five months, fully closed. To best our knowledge, this first case application felines, provides further evidence effectiveness approach. successful outcome paves way treatments against persistent practice.

Language: Английский

Citations

6
Protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections DOI Creative Commons
Hadil Onallah, Ortal Yerushalmy,

Ron Braunstein

et al.

STAR Protocols, Journal Year: 2024, Volume and Issue: 5(2), P. 102949 - 102949

Published: April 30, 2024

Phage therapy has re-emerged as a promising treatment for non-resolving infections. Given the lack of approved phage treatments, there is need to establish compassionate use pipeline. Here, we present protocol matching, treatment, and monitoring bacteriophage in We describe steps consultation request implementation, evaluating comparing different aspects activity, production. then detail procedures multidisciplinary meetings, ethics approvals, therapy, follow-up. For complete details on execution this protocol, please refer Onallah et al.1,2

Language: Английский

Citations

5
Evaluation of the Ratio of Spot Tests and Plaque-Forming Activity Tests of Bacteriophages of Prevalent Pathogens DOI Creative Commons
Ivan M. Pchelin, Daniil V. Azarov,

V. A. Dedik

et al.

Epidemiology and Vaccinal Prevention, Journal Year: 2025, Volume and Issue: 23(6), P. 129 - 136

Published: Jan. 15, 2025

Relevance . Obtaining clean lysis spots, with tolerable presence of individual colonies secondary growth, in spot tests is a criterion for assessing the effectiveness therapeutic bacteriophages. At same time, number mechanisms are known by which phage bacteria on lawns occurs without being accompanied virus replication. Aim To assess ratio test results and based detection negative bacteriophage colonies. Conclusion Data 43 bacteriophages extracted from 21 articles were analyzed. Within studied sample, observation zones 94% cases corresponded to successful For Escherichia coli bacteriophages, greater "++++" category detected compared strains supporting replication, which, within framework lytic activity bacteriophage, can be characterized as false positive results. In general, clear spots most indicates allows us consider method indicative, but requiring subsequent validation more complex methods characterizing efficiency

Language: Английский

Citations

0
Phage Therapy to Treat Unresponsive Infections: a Primer for the Clinical Microbiology Laboratory Staff DOI Creative Commons

Josephine M. Davey-Young,

Dinuri D. Punchihewa,

Bernadette Ng

et al.

Clinical Microbiology Newsletter, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
‘French Phage Network’ annual conference - eighth meeting report DOI Creative Commons

Carole Armal,

Catherine Arnaud,

Émilie Cenraud

et al.

microLife, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 1, 2025

The French Phage Network organizes a scientific meeting every year in which the community of researchers from academia and industry, as well clinicians participate due to growing interest phage therapy. Although centered on giving exposure future generations scientists with senior investigators invited main speakers, has also welcomed participants other countries. Covering almost aspect bacteriophage biology, is an opportunity not only expose youngest broad range topics, but share their most recent "work progress" without undergoing stringent selection process obtain oral presentation slot. This report reflects dynamism research field bacteriophages across multiple disciplines, including molecular structural ecology, evolution, therapy, biotechnology.

Language: Английский

Citations

0
Clinical Application of Bacteriophage Therapy in Children DOI
Nanda Ramchandar,

John S. Bradley

PEDIATRICS, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Bacteriophages are highly specific in targeting single pathogens the treatment of bacterial infections and thus offer a promising alternative to antibiotic therapies with potential for essentially no impact child’s own microbiome. have been evaluated by investigators several tissue site adults, but data children lacking. The application this therapy pediatrics provides an extraordinary opportunity redesign our approach directed, precision medicine antimicrobial infants both acute chronic infections, particularly those caused multidrug-resistant pathogens. In therapeutic approach, bacteriophage preparation would be selected exclusively limited patient’s pathogen, minimizing collateral damage microbiome, as often demonstrated therapy. following article, we describe these novel anti-infective biological agents, review recent literature, discuss practical considerations limitations, share applications topical systemic administration bacteriophages, either primary or combination traditional antibiotics. Additionally, important areas interest pediatric research discussed.

Language: Английский

Citations

0
Six Novel Pseudomonas aeruginosa Phages: Genomic Insights and Therapeutic Potential DOI Open Access
Amit Rimon, Ortal Yerushalmy, Jonathan Belin

et al.

PHAGE, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Introduction: Pseudomonas aeruginosa is an opportunistic pathogen that causes health care-associated infections. The rise of antibiotic-resistant bacterial strains necessitates alternative treatment strategies, with bacteriophage therapy being a promising approach.

Language: Английский

Citations

0