Exploring the potential of seed inoculation with microbial consortia to mitigate drought stress in maize plants under greenhouse conditions

Plant and Soil, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract Background and aims Plant-beneficial microbes may attenuate climate change-induced stresses on plants such as drought. We investigated the potential of beneficial microbial consortia (BMc) plant growth rhizosphere bacterial/archaeal community under Methods Seeds Zea mays B73 were inoculated with six plant-beneficial bacterial isolates either alone or combined in two three-member (BMc1, BMc2) before sowing loamy sandy substrates greenhouse. A known effective consortium (BMc3) was included positive control. Drought treatment established BMc treatments by omitting watering last five weeks period. The maize single determined. Colony-forming units (CFUs) inoculants evaluated selective plating, effects native assessed using 16S rRNA gene amplicon sequencing basal root tip grown loam. Results In both water conditions, CFUs inoculations higher at roots than tips. Under well-watered seed inoculation a isolate had no effect substrates. resulted shoot (but not root) compared to non-inoculated controls conditions zone most important driver for beta-diversity, followed while showed effect. Conclusion Our study suggests that has drought stress during growth.

Language: Английский

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Plant-Soil Microbial Interaction: Differential Adaptations of Beneficial vs. Pathogenic Bacterial and Fungal Communities to Climate-Induced Drought and Desiccation Stresses

Published: July 16, 2024

Climate change and the increasing frequency severity of drought events pose significant challenges for sustainable agriculture worldwide. Soil microorganisms, both beneficial pathogenic, play a crucial role in mediating plant-environment interactions shaping overall functioning agroecosystems. This review synthesizes current knowledge on contrasting adaptive mechanisms utilized by different groups plant-soil microorganisms focusing pathogenic bacterial fungal communities response to desiccation stresses. The examines common survival strategies employed microbes specifically rhizobacteria arbuscular mycorrhizal fungi, such as production osmoprotectants, altered gene expression, biofilm formation. It also highlights distinct versus mutualistic microbes, with pathogens tending prioritize virulence factors suppress plant growth, while enhance growth stress tolerance. Genetic exchange horizontal transfer (HGT) is identified key mechanism, allowing non-pathogenic acquire traits like tolerance factors. Environmental stressors can promote increased genetic spread within soil microbiome. complex interplay between drought-adapted their plants discussed, emphasizing need deeper understanding microbiome dynamics under climate change. be agricultural practices mitigate impacts health productivity. provides insights into divergent desiccation, managing resilience agroecosystems

Language: Английский

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Plant–Soil Microbial Interaction: Differential Adaptations of Beneficial vs. Pathogenic Bacterial and Fungal Communities to Climate-Induced Drought

Agronomy, Journal Year: 2024, Volume and Issue: 14(9), P. 1949 - 1949

Published: Aug. 29, 2024

Climate change and the increasing frequency severity of drought events pose significant challenges for sustainable agriculture worldwide. Soil microorganisms, both beneficial pathogenic, play a crucial role in mediating plant–environment interactions shaping overall functioning agroecosystems. This review summarizes current knowledge on adaptive mechanisms used by different groups plant-beneficial soil microorganisms—rhizobacteria arbuscular mycorrhizal fungi (AMF)—as well as phytopathogenic bacteria fungi, response to drought. The focuses identifying commonalities differences survival strategies these pathogenic microorganisms under conditions. Additionally, it reviews compares plant defence conditions facilitated rhizobacteria AMF. Special attention is given genetic exchange between through horizontal gene transfer (HGT), which allows them traits. It observed that may favor enhanced spread traits microbiome. will be useful wide range readers better understand dynamics microbiome climate apply this agricultural practices.

Language: Английский

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Effect of various methods of mineral fertilizer application on changes in the abundance, composition, and diversity of culturable fungi in a reduced tillage crop rotation system of winter wheat, soybean, and maize

Agriculture Ecosystems & Environment, Journal Year: 2025, Volume and Issue: 386, P. 109589 - 109589

Published: March 11, 2025

Language: Английский

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Gypsum and Tillage Practices for Combating Soil Salinity and Enhancing Crop Productivity

Agriculture, Journal Year: 2025, Volume and Issue: 15(6), P. 658 - 658

Published: March 20, 2025

Soil salinity is a major global challenge, reducing fertility and crop productivity. This study evaluated the effects of various soil management practices on physical, chemical, microbial properties saline soils. Six treatments, combining loosening, ploughing, disking, gypsum amendment, were applied to solonetzic meadow with high sodium levels. penetration resistance was measured using Penetronik penetrometer, while chemical analyses included pH, total salt content, calcium carbonate (CaCO3), humus, exchangeable cations (Na+, K+, Ca2+, Mg2+). Microbial composition determined through DNA extraction nanopore sequencing. The results showed that level A had lowest (333 ± 200 N/m2), indicating better conditions for plant growth. Gypsum loosening treatment significantly improved (141 N/m2, p < 0.001), amendment enhanced (p 0.05, 0.01, 0.001). application balanced parameters influenced communities. Reduced tillage favored functionally important genera but did not support fungal diversity > 0.05). These findings highlight effectiveness practices, like in mitigating stress fostering beneficial Combining these methods proved most effective enhancing health, offering insights sustainable environments.

Language: Английский

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Fragile foundations: Succession patterns of bacterial communities in fine woody debris and soil under long-term microclimate influence

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Background Fine woody debris (FWD) is a crucial yet often overlooked component of forest ecosystems, providing dynamic habitat for microbial communities and playing key role in carbon nutrient cycling. In managed forests with low deadwood stocks, FWD decomposition enhances soil fertility by facilitating Climate change increases the prevalence disturbances enhancing area early succession canopy cover, but consequences on related processes insufficiently understood. Results Here we conducted ten-year experiment manipulating cover to examine Fagus sylvatica Abies alba. Our study revealed that openness significantly affected bacterial diversity decomposing wood as well surrounding soil. While community structure was primarily influenced time, tree species density also played role. We identified taxa associated carbohydrate utilization, fungal biomass degradation, nitrogen fixation, highlighting diverse functional roles bacteria Bacterial almost completely decomposed remains clearly distinct from communities. Conclusions Complex ecological interactions shape The interplay between species, microclimatic variability influences dynamics, acting more stable decomposer compared previously studied fungi. This stability may be critical sustaining turnover despite environmental fluctuations global change.

Language: Английский

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Environmental DNA as a tool for soil health monitoring and unveiling new ecological frontiers

Ecological Indicators, Journal Year: 2025, Volume and Issue: 174, P. 113438 - 113438

Published: April 9, 2025

Language: Английский

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Impacts of long-term irrigation of municipally-treated wastewater to the soil microbial and nutrient properties

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 959, P. 178143 - 178143

Published: Dec. 24, 2024

Language: Английский

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Prolonged water limitation shifts the soil microbiome from copiotrophic to oligotrophic lifestyles in Scots pine mesocosms

Environmental Microbiology Reports, Journal Year: 2023, Volume and Issue: 16(1)

Published: Nov. 22, 2023

Abstract Reductions in soil moisture due to prolonged episodes of drought can potentially affect whole forest ecosystems, including microorganisms and their functions. We investigated how the composition microbial communities is affected by water limitation. In a mesocosm experiment with Scots pine saplings natural maintained at different levels content over 2 years, we assessed shifts prokaryotic fungal related these changes plant development properties. Prolonged limitation induced progressive community composition. The dissimilarity between increased time regardless recurrent seasons, while were less Under low contents, desiccation‐tolerant groups outcompeted adapted, lifestyle taxa shifted from copiotrophic oligotrophic. While abundance saprotrophic ligninolytic alongside an accumulation dead material, symbiotic nutrient‐cycling decreased, likely impairing trees. Overall, appeared continuously alter structure communities, pointing potential loss critical functions provided microbiome.

Language: Английский

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Water limitation intensity shifts carbon allocation dynamics in Scots pine mesocosms

Plant and Soil, Journal Year: 2023, Volume and Issue: 490(1-2), P. 499 - 519

Published: June 17, 2023

Abstract Background and aims Tree species worldwide suffer from extended periods of water limitation. These conditions not only affect the growth vitality trees but also feed back on cycling carbon (C) at plant-soil interface. However, impact progressing loss soils transfer assimilated C belowground remains unresolved. Methods Using mesocosms, we assessed how increasing levels deficit Pinus sylvestris saplings performed a 13 C-CO 2 pulse labelling experiment to trace pathway into needles, fine roots, soil pore CO 2, phospholipid fatty acids microbial groups. Results With limitation, partitioned more biomass expense aboveground growth. Moderate limitation barely affected uptake label transit time needles . Comparatively, severe increased fraction that allocated roots fungi while lower was readily respired soil. Conclusions When becomes largely unavailable, within slower, may accumulate in or be transferred associated microorganisms without being metabolically used.

Language: Английский

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