The MYC2–PUB22–JAZ4 module plays a crucial role in jasmonate signaling in tomato

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(4), P. 598 - 613

Published: Feb. 9, 2024

Jasmonates (JAs), a class of lipid-derived stress hormones, play crucial role across an array plant physiological processes and responses. Although JA signaling is thought to rely predominantly on the degradation specific JAZ proteins by SCFCOI1, it remains unclear whether other pathways are involved in regulation protein stability. Here, we report that PUB22, U-box type E3 ubiquitin ligase, plays critical resistance against Helicoverpa armigera responses tomato. Whereas COI1 physically interacts with JAZ1/2/5/7, PUB22 JAZ1/3/4/6. ubiquitinates JAZ4 promote its via 26S proteasome pathway. Importantly, observed pub22 mutants showreduced H. armigera, whereas jaz4 single jaz1 jaz3 jaz6 quadruple have enhanced resistance. The hypersensitivity herbivores could be partially rescued mutation. Moreover, found expression can transcriptionally activated MYC2, thus forming positive feedback circuit signaling. We noticed PUB22–JAZ4 module also regulates responses, including defense B. cinerea, inhibition root elongation, anthocyanin accumulation. Taken together, these results indicate growth together COI1-regulated signaling, targeting JAZs.

Language: Английский

---

Brassinosteroid signaling regulator BIM1 integrates brassinolide and jasmonic acid signaling during cold tolerance in apple

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 193(2), P. 1652 - 1674

Published: July 1, 2023

Although brassinolide (BR) and jasmonic acid (JA) play essential roles in the regulation of cold stress responses, molecular basis their crosstalk remains elusive. Here, we show a key component BR signaling apple (Malus × domestica), INSENSITIVE1 (BRI1)-EMS-SUPPRESSOR1 (BES1)-INTERACTING MYC-LIKE PROTEIN1 (MdBIM1), increases tolerance by directly activating expression C-REPEAT BINDING FACTOR1 (MdCBF1) forming complex with FACTOR2 (MdCBF2) to enhance MdCBF2-activated transcription cold-responsive genes. Two repressors JA signaling, JAZMONATE ZIM-DOMAIN1 (MdJAZ1) ZIM-DOMAIN2 (MdJAZ2), interact MdBIM1 integrate under stress. MdJAZ1 MdJAZ2 reduce MdBIM1-promoted attenuating transcriptional activation MdCBF1 interfering formation MdBIM1-MdCBF2 complex. Furthermore, E3 ubiquitin ligase ARABIDOPSIS TÓXICOS en LEVADURA73 (MdATL73) decreases targeting for ubiquitination degradation. Our results not only reveal between mediated JAZ-BIM1-CBF module but also provide insights into posttranslational regulatory mechanism signaling.

Language: Английский

---

IbNIEL‐mediated degradation of IbNAC087 regulates jasmonic acid‐dependent salt and drought tolerance in sweet potato

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: 66(2), P. 176 - 195

Published: Jan. 31, 2024

ABSTRACT Sweet potato ( Ipomoea batatas [L.] Lam.) is a crucial staple and bioenergy crop. Its abiotic stress tolerance holds significant importance in fully utilizing marginal lands. Transcriptional processes regulate responses, yet the molecular regulatory mechanisms sweet remain unclear. In this study, NAC (NAM, ATAF1/2, CUC2) transcription factor, IbNAC087 , was identified, which commonly upregulated salt‐ drought‐tolerant germplasms. Overexpression of increased salt drought by increasing jasmonic acid (JA) accumulation activating reactive oxygen species (ROS) scavenging, whereas silencing gene resulted opposite phenotypes. JA‐rich ‐OE (overexpression) plants exhibited more stomatal closure than wild‐type (WT) ‐Ri under NaCl, polyethylene glycol, methyl jasmonate treatments. functions as nuclear transcriptional activator directly activates expression key JA biosynthesis‐related genes lipoxygenase IbLOX ) allene oxide synthase IbAOS ). Moreover, physically interacted with RING‐type E3 ubiquitin ligase NAC087‐INTERACTING LIGASE (IbNIEL), negatively regulating potato. IbNIEL ubiquitinated to promote 26S proteasome degradation, weakened its activation on . The findings provide insights into mechanism underlying IbNIEL‐IbNAC087 module regulation JA‐dependent response candidate for improving crops.

Language: Английский

---

Functions of Basic Helix–Loop–Helix (bHLH) Proteins in the Regulation of Plant Responses to Cold, Drought, Salt, and Iron Deficiency: A Comprehensive Review

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(19), P. 10692 - 10709

Published: May 7, 2024

Abiotic stresses including cold, drought, salt, and iron deficiency severely impair plant development, crop productivity, geographic distribution. Several bodies of research have shed light on the pleiotropic functions BASIC HELIX–LOOP–HELIX (bHLH) proteins in responses to these abiotic stresses. In this review, we mention regulatory roles bHLH TFs response such as salt resistance, deficiency, well enhancing grain yield plants, especially crops. The bind E/G-box motifs target promoter interact with various other factors form a complex network. Through network, they cooperatively activate or repress transcription downstream genes, thereby regulating stress responses. Finally, present some perspectives for future focusing molecular mechanisms that integrate coordinate Understanding is crucial development stress-tolerant

Language: Английский

---

Deciphering the mechanism of E3 ubiquitin ligases in plant responses to abiotic and biotic stresses and perspectives on PROTACs for crop resistance

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: unknown

Published: June 12, 2024

With global climate change, it is essential to find strategies make crops more resistant different stresses and guarantee food security worldwide. E3 ubiquitin ligases are critical regulatory elements that gaining importance due their role in selecting proteins for degradation the ubiquitin-proteasome proteolysis pathway. The of Ub has been demonstrated numerous cellular processes plants responding biotic abiotic stresses. considered a class difficult control by conventional inhibitors, as they lack standard active site with pocket, biological activity mainly protein-protein interactions transient conformational changes. Proteolysis-targeted chimeras (PROTACs) new heterobifunctional molecules have emerged recent years relevant alternatives incurable human diseases like cancer because can target recalcitrant destruction. PROTACs interact system, principally ligase cell, facilitate proteasome turnover interest. PROTAC harness functions proteasomal involved dysfunction. This review examines advances research plant responses It highlights how be applied stress response mitigate pathogenic agents environmental adversities.

Language: Английский

---

E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against Pseudomonas syringae pv. actinidiae

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1930 - 1930

Published: Feb. 5, 2024

Kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) is the most serious disease threatening kiwifruit production. Our previous study found genes encoding U-box containing proteins were significantly regulated Psa infection. Here, we report a type E3 ubiquitin ligase PUB23 in which acts as negative regulator of immune responses against Psa. was to physically interact with GT1, trihelix transcription factor, vitro and vivo. The expression GT1 up-regulated PUB23-silenced plants, indicating that interacting may directly or indirectly suppress expression. silencing led enhanced PAMP-triggered immunity (PTI), including higher level defense marker PR1 RIN4, increased accumulation hydrogen peroxide superoxide anion. results reveal role plays regulate gene GT1.

Language: Английский

---

Understanding cold stress response mechanisms in plants: an overview

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 6, 2024

Low-temperature stress significantly impacts plant growth, development, yield, and geographical distribution. However, during the long-term process of evolution, plants have evolved complicated mechanisms to resist low-temperature stress. The cold tolerance trait is regulated by multiple pathways, such as Ca 2+ signaling cascade, mitogen-activated protein kinase (MAPK) inducer CBF expression 1 (ICE1)-C-repeat binding factor (CBF)-cold-reulated gene (COR) transcriptional reactive oxygen species (ROS) homeostasis regulation, hormone signaling. specific responses these pathways their interactions are not fully understood. This review summarizes response from four aspects, including signal perception transduction, ICE1-CBF-COR transcription cascade ROS regulation regulation. It also elucidates mechanism transduction in plants, proposes important roles factors (TFs), post-translational modifications (PTMs), light signals, circadian clock factors, interaction proteins cascade. Additionally, we analyze importance response, explore cross interconnections among homeostasis, comprehensive enhances our understanding provides a molecular basis for genetic strategies improve tolerance.

Language: Английский

---

Apple E3 ligase MdPUB23 mediates ubiquitin-dependent degradation of MdABI5 to delay ABA-triggered leaf senescence

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(4)

Published: Jan. 30, 2024

ABSCISIC ACID-INSENSITIVE5 (ABI5) is a core regulatory factor that mediates the ABA signaling response and leaf senescence. However, molecular mechanism underlying synergistic regulation of senescence by ABI5 with interacting partners homeostasis in remain to be further investigated. In this study, we found accelerated effect MdABI5 on partly dependent MdbHLH93, an activator apple. directly interacted MdbHLH93 improved transcriptional activation senescence-associated gene MdSAG18 MdbHLH93. MdPUB23, U-box E3 ubiquitin ligase, physically delayed ABA-triggered Genetic biochemical analyses suggest MdPUB23 inhibited MdABI5-promoted premature targeting for ubiquitin-dependent degradation. conclusion, our results verify accelerates through MdABI5-MdbHLH93-MdSAG18 module, responsible dynamic modulating MdABI5.

Language: Английский

---

Genome-Wide Identification and Expression Analysis of the PUB Gene Family in Zoysia japonica under Salt Stress

Plants, Journal Year: 2024, Volume and Issue: 13(6), P. 788 - 788

Published: March 10, 2024

The U-box protein family of ubiquitin ligases is important in the biological processes plant growth, development, and biotic abiotic stress responses. Plants genus Zoysia are recognized as excellent warm-season turfgrass species with drought, wear salt tolerance. In this study, we conducted genome-wide identification (PUB) genes japonica based on domain searching. total, 71 ZjPUB were identified, a tree was constructed AtPUBs, OsPUBs, ZjPUBs, clustered into five groups. gene structures, characteristics, cis-elements interaction prediction network analyzed. There mainly ABRE, ERE, MYB MYC distributed promoter regions ZjPUBs. ZjPUBs predicted to interact PDR1 EXO70B1, related abscisic acid signaling pathway. To better understand roles under stress, expression levels 18 detected using transcriptome data qRT-PCR analysis, revealing that 16 upregulated roots treatment. This indicates might participate Z. response. research provides insight PUB may support genetic improvement molecular breeding salt-tolerant zoysiagrass varieties.

Language: Английский

---

Pepper U‐Box E3 ubiquitin ligase 24, CaPUB24, negatively regulates drought stress response

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(2)

Published: March 1, 2024

Abstract Under stress conditions, plants modulate their internal states and initiate various defence mechanisms to survive. The ubiquitin‐proteasome system is one of the critical modules in these mechanisms, Plant U‐Box proteins play an important role this process as E3 ubiquitin ligases. Here, we isolated U‐box 24 gene CaPUB24 ( C apsicum a nnuum P lant U ‐ B ox ) from pepper characterized its functions response drought stress. We found that, compared other CaPUBs same group, expression was significantly induced by also that localized nucleus cytoplasm had ligase activity. To investigate biological further, generated ‐silenced ‐overexpressing Arabidopsis transgenic plants. exhibited enhanced tolerance control due reduced transpirational water loss increased abscisic acid (ABA) sensitivity. In contrast, ABA‐insensitive phenotypes. Our findings suggest negatively modulates ABA‐dependent manner.

Language: Английский

---