International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 268, P. 131804 - 131804
Published: April 25, 2024
Language: Английский
Nature Plants, Journal Year: 2024, Volume and Issue: 10(4), P. 551 - 566
Published: March 20, 2024
Language: Английский
Citations
33
Nature Genetics, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 26, 2025
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: March 27, 2024
Abstract Telomeres play an important role in chromosome stability and their length is thought to be related organism’s lifestyle lifespan. Telomere variable across plant species between cultivars of the same species, possibly conferring adaptive advantage. However, it not known whether telomere or life span a diverse array due lack information on plants. Here we leverage genomes assembled with long read sequencing data estimate by chromosome. We find that assemblies based Oxford Nanopore Technologies (ONT) accurately predict two model Arabidopsis thaliana Oryza sativa matching lab-based estimates. then different lifestyles lifespans general gymnosperms have shorter telomeres compared eudicots monocots. Crop frequently longer than wild relatives, been maintained clonally such as hemp reflecting this requires term chromosomal stability.
Language: Английский
Citations
5
Horticulture Research, Journal Year: 2024, Volume and Issue: 11(7)
Published: May 7, 2024
Abstract The grape genome is the basis for studies and breeding, also important industries. In last two decades, more than 44 genomes have been sequenced. Based on these genomes, researchers made substantial progress in understanding mechanism of biotic abiotic resistance, berry quality formation, breeding strategies. addition, this work has provided essential data future pangenome analyses. Apart from de novo assembled six whole-genome sequencing projects datasets comprising almost 5000 accessions. datasets, explored domestication origins clarified gene flow that occurred during its dispersed history. Moreover, genome-wide association other methods used to identify 900 genes related quality, developmental phases grape. These findings benefited provide some smart genomic selection breeding. played a great role industry, importance genomics will increase sharply future.
Language: Английский
Citations
4
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: March 2, 2024
Grapevine (Vitis) is the oldest domesticated fruit crop with great cultural and economic importance. Here, we assemble annotate haplotype-resolved genomes of 72 Vitis accessions including 25 wild 47 cultivated grapevines, a complete genome V. vinifera. Coalescent phylogenomics 142 haplotype disentangles mysterious hybridization history revealing enormous genetic diversity among species. Pangenome analysis together phenotyping data reveals that European cultivars, more susceptible to most destructive disease downy mildew (DM), had smaller repertoire resistance genes NLR family. Through extensive structural variation (SV) characterization, phenotyping, transcriptome profiling 113 accessions, SV-eQTL analysis, have identified over 79 SVs their relevant significantly associated DM resistance, exemplified by lysine histidine transporter, VvLHT8. This pangenome genus will accelerate grapevine breeding enrich our understanding evolution biology grapevines.
Language: Английский
Citations
3
Horticultural Plant Journal, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
0
Cell Reports, Journal Year: 2025, Volume and Issue: 44(5), P. 115621 - 115621
Published: May 1, 2025
Tartary buckwheat (TB), Fagopyrum tataricum, is an important medicinal and edible crop with a worldwide distribution. However, reference genomes gaps mutant population scarcity have hindered functional genomics genetic improvement of TB. Here, we present telomere-to-telomere (T2T) gap-free genome assembly the elite TB inbred line Guiku1 its ethyl-methyl-sulfonate (EMS)-induced phenotypically rich mutation library. The spans 453.83 Mb, containing 43,441 predicted protein-coding genes. library includes 751 mutants stably heritable phenotypes. Whole-genome resequencing 320 identified 105,682 single-nucleotide polymorphisms (SNPs) 21,461 insertions/deletions (indels), affecting sequences 25,986 Genes responsible for pink stem petiole trait flavonoid content variation were using forward- reverse-genetics approaches, respectively. Collectively, T2T EMS provide resources studies
Language: Английский
Citations
0
The Plant Journal, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 8, 2024
SUMMARY The implementation of genome editing strategies in grapevine is the easiest way to improve sustainability and resilience while preserving original genotype. Among others, Mildew Locus‐O ( MLO ) genes have already been reported as good candidates develop powdery mildew‐immune plants. A never‐explored target NPR3 , a negative regulator systemic acquired resistance. We report exploitation cisgenic approach with Cre‐lox recombinase technology generate grapevine‐edited plants potential be transgene‐free their genetic background. characterization three edited lines for each demonstrated immunity development against Erysiphe necator MLO6‐7 ‐edited Concomitantly, significant improvement resilience, associated increased leaf thickness specific biochemical responses, was observed defective E. Plasmopara viticola . Transcriptomic analysis revealed that both modulated gene expression profiles, pointing distinct though partially overlapping responses. Furthermore, targeted metabolite highlighted an overaccumulation stilbenes coupled improved oxidative scavenging targets, likely protecting mutants from detrimental pleiotropic effects. Finally, Cre‐loxP allowed recovery one plant complete removal transgene. Taken together, our achievements provide comprehensive understanding molecular adjustments occurring double ‐defective grape In parallel, multiple purposes has demonstrated, raising new questions on its wide role orchestrating biotic stress
Language: Английский
Citations
3
Horticulture Research, Journal Year: 2024, Volume and Issue: 11(6)
Published: April 16, 2024
The economically significant genus Prunus includes fruit and nut crops that have been domesticated for shared specific agronomic traits; however, the genomic signals of convergent divergent selection not elucidated. In this study, we aimed to detect signatures by conducting comparative population analyses apricot-peach-plum-mei (APPM) complex, utilizing a haplotype-resolved telomere-to-telomere (T2T) genome assembly resequencing data. T2T reference plum cultivar was assembled through HiFi Hi-C reads, resulting in two haplotypes 251.25 251.29 Mb size, respectively. Comparative genomics reveals chromosomal translocation ~1.17 apricot genomes compared with peach, plum, mei. Notably, involves D locus, significantly impacting titratable acidity (TA), pH, sugar content. Population genetic analysis detected substantial gene flow between apricot, introgression regions enriched post-embryonic development pollen germination processes. revealed stress tolerance, flower development, ripening, along shaping crop, such as somatic embryogenesis mei, hormone regulation peach. selective sweeps on chromosome 7 coincide collinearity from genomics, key fruit-softening genes PG, regulated ERF RMA1H1. Overall, study provides insights into diversity, evolutionary history, domestication APPM offering valuable implications studies breeding programs crops.
Language: Английский
Citations
2
Agronomy, Journal Year: 2024, Volume and Issue: 15(1), P. 48 - 48
Published: Dec. 27, 2024
Grapevines are widely cultivated and highly nutritious, making them economically significant among plants globally. However, important spring temperature fluctuations frequent frost events in some production areas severely affect grape growth yield. Therefore, enhancing the cold resistance of grapevines is considerable importance for production. This study used less cold-resistant varieties Vitis vinifera cv. Cabernet Sauvignon Chardonnay as test materials. They were treated with antifreeze agents ‘GuoGuang KangXiu,’ ‘Biogas Liquid’, ‘SenHuo’, ‘Ice Shield’ 24 48 h before exposure to a low-temperature treatment −2 °C. Compared control (water), all four significantly reduced relative conductivity malondialdehyde content tender shoots both varieties, increased Fv/Fm ratio, enhanced osmotic adjustment substance levels, improved antioxidant enzyme activity. Moreover, lower, ratio was higher when applied compared h. suggests that effectively improve grapevine resistance, better results observed frost. Additionally, soluble solid berries levels anthocyanins, total phenols, tannins, flavonoids, flavonols berries’ skin. had minimal impact on fruit set rate, cluster weight, shape index, indicating these can quality extent.
Language: Английский
Citations
1