Exploring the impact of environmental factors on male reproductive health through epigenetics

Reproductive Toxicology, Journal Year: 2025, Volume and Issue: 132, P. 108832 - 108832

Published: Jan. 6, 2025

Language: Английский

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Single-cell transcriptomic atlas of the human testis across the reproductive lifespan

Nature Aging, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Testicular aging is associated with declining reproductive health, but the molecular mechanisms are unclear. Here we generate a dataset of 214,369 single-cell transcriptomes from testicular cells 35 individuals aged 21–69, offering resource for studying and physiology. Machine learning analysis reveals stronger response in somatic compared to germ cells. Two waves aging-related changes identified: first peritubular donors their 30s, marked by increased basement membrane thickness, indicating priming state aging. In 50s, exhibit functional changes, including altered steroid metabolism Leydig immune responses macrophages. Further analyses reveal impact body mass index on spermatogenic capacity as age progresses, particularly after 45. Altogether, our findings illuminate alterations during testis relationship index, providing foundation future research potential diagnostic markers therapeutic targets. To enhance understanding human testis, authors provide map characteristics throughout lifespan. Using samples donors, they identify two examine interactions between index.

Language: Английский

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Male factor infertility and assisted reproductive technologies: indications, minimum access criteria and outcomes

Journal of Endocrinological Investigation, Journal Year: 2023, Volume and Issue: 46(6), P. 1079 - 1085

Published: Jan. 12, 2023

Abstract Background Infertility, which is defined as the inability to conceive after at least 12 months of regular unprotected sexual intercourses, affects about 15–20% couples worldwide and a male factor involved in half cases. The development assisted reproductive technology (ART) made it possible also individuals affected from severe oligospermia or azoospermia. However, impact on embryo development, implantation, prevalence chromosomal abnormalities, genetic epigenetic alterations, clinical obstetric outcomes still controversial. Purpose This narrative review examines indications, minimum access criteria, by individual ART technique relation factor.

Language: Английский

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Endocrine disrupting chemicals and male fertility: from physiological to molecular effects

Frontiers in Public Health, Journal Year: 2023, Volume and Issue: 11

Published: Oct. 10, 2023

The deleterious effects of chemical or non-chemical endocrine disruptors (EDs) on male fertility potential is well documented but still not fully elucidated. For example, the detection industrial chemicals' metabolites in seminal plasma and follicular fluid can affect efficiency gametogenesis, maturation competency gametes has guided scientists to hypothesize that disrupting chemicals (EDCs) may disrupt hormonal homoeostasis by leading a wide range control impairments. EDCs exposure reproductive health are highly dependent factors including type EDCs, duration exposure, individual susceptibility, presence other co-factors. Research continue study these complex interactions. aim this review summarize literature better understand risks France.

Language: Английский

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Male reproductive ageing: a radical road to ruin

Human Reproduction, Journal Year: 2023, Volume and Issue: 38(10), P. 1861 - 1871

Published: Aug. 10, 2023

In modern post-transition societies, we are reproducing later and living longer. While the impact of age on female reproductive function has been well studied, much less is known about intersection male reproduction. Our current understanding that advancing brings forth a progressive decline in fertility accompanied by reduction circulating testosterone levels appearance age-dependent pathologies including benign prostatic hypertrophy erectile dysfunction. Paternal ageing also associated with profound increase sperm DNA damage, multiple epigenetic changes germ line an elevated mutational load offspring. The net result such disease burden carried progeny males, dominant genetic diseases as Apert syndrome achondroplasia, neuropsychiatric conditions autism spontaneous schizophrenia. basis these age-related effects appears to involve two fundamental mechanisms. first positive selection mechanism whereby stem cells containing mutations mitogen-activated protein kinase pathway gain selective advantage over their non-mutant counterparts exhibit significant clonal expansion passage time. second dependent oxidative stress which impairs steroidogenic capacity Leydig cells, disrupts ability Sertoli support normal differentiation functional integrity spermatozoa. Given central importance defining chronological reproduction, there may be role for antioxidants clinical management this process. animal studies supportive strategy, carefully designed trials now needed if realize therapeutic potential approach context.

Language: Английский

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Advanced Paternal Age in Focus: Unraveling Its Influence on Assisted Reproductive Technology Outcomes

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(10), P. 2731 - 2731

Published: May 7, 2024

As global demographics shift toward increasing paternal age, the realm of assisted reproductive technologies (ARTs), particularly in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), faces new challenges opportunities. This study provides a comprehensive exploration implications advanced age on ART outcomes. Background research highlights social, cultural, economic factors driving men later fatherhood, with focus impact delayed paternity Methods involve thorough review existing literature, centering changes testicular function, semen quality, genetic epigenetic shifts associated advancing age. Study results point to intricate associations between father’s outcomes, older being linked diminished potential risks, varied impacts embryo implantation rates, birth The conclusions drawn from current suggest that while presents certain risks challenges, understanding mitigating these through strategies such as cryopreservation, lifestyle modifications, preimplantation testing can optimize Future directions are identified further comprehend mechanisms long-term effects father offspring health. underscores need for approach navigating intricacies fatherhood within context ART, aiming best possible outcomes couples their children.

Language: Английский

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Paternal Age Matters: Association with Sperm Criteria’s- Spermatozoa DNA Integrity and Methylation Profile

Journal of Clinical Medicine, Journal Year: 2023, Volume and Issue: 12(15), P. 4928 - 4928

Published: July 27, 2023

Advanced age has been reported to negatively affect sperm parameters and spermatozoa DNA integrity. A decline in criteria was also associated with altered epigenetic marks such as methylation a potential downstream impact on vitro fertilization success clinical outcomes. The aim of the present retrospective study clarify association between advanced paternal (APA) parameters, integrity profile. total 671 patients consulting for infertility underwent analysis, assessment level measurement. principal finding that individuals over 40 years exhibit significant increase fragmentation levels compared younger group (15% versus 9%, respectively,

Language: Английский

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Oxidative Stress Biomarkers in Male Infertility: Established Methodologies and Future Perspectives

Genes, Journal Year: 2024, Volume and Issue: 15(5), P. 539 - 539

Published: April 25, 2024

Male fertility can be affected by oxidative stress (OS), which occurs when an imbalance between the production of reactive oxygen species (ROS) and body's ability to neutralize them arises. OS damage cells influence sperm production. High levels lipid peroxidation have been linked reduced motility decreased fertilization ability. This literature review discusses most commonly used biomarkers measure caused ROS, such as high level in seminal plasma indicator antioxidant activity. The investigated include 8-hydroxy-2-deoxyguanosine acid (8-OHdG), a marker DNA F2 isoprostanoids (8-isoprostanes) produced peroxidation. Furthermore, this focuses on recent methodologies including NGS polymorphisms differentially expressed gene (DEG) analysis, well epigenetic mechanisms ROS during spermatogenesis along with new developed evaluate biomarkers. Finally, addresses valuable insight into male infertility provided these advances how they led treatment possibilities. Overall, use has supplied innovative diagnostic therapeutic approaches, enhancing our understanding mechanisms.

Language: Английский

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H19 Sperm Methylation in Male Infertility: A Systematic Review and Meta-Analysis

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7224 - 7224

Published: April 13, 2023

This systematic review and meta-analysis summarize the difference in methylation of H19 gene patients with abnormal versus normal conventional sperm parameters. It also evaluates effects age concentration on spermatozoa using meta-regression analysis. was performed according to MOOSE guidelines for meta-analyses Systematic Reviews Observational Studies Preferred Reporting Items Review Meta-Analysis Protocols (PRISMA-P). The quality evidence reported studies included assessed Cambridge Quality Checklists. A total 11 articles met our inclusion criteria. Quantitative analysis showed that levels were significantly lower group infertile than fertile controls. reduction much more pronounced oligozoospermia (alone or associated other parameter abnormalities) those recurrent pregnancy loss. Meta-regression results be independent both patient concentration. Therefore, pattern should evaluated among couples accessing assisted reproductive techniques (ART), order gain prognostic information ART outcome offspring health.

Language: Английский

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Paternal effects on fetal programming

Animal Reproduction, Journal Year: 2023, Volume and Issue: 20(2)

Published: Jan. 1, 2023

Paternal programming is the concept that environmental signals from sire's experiences leading up to mating can alter semen and ultimately affect phenotype of resulting offspring. Potential mechanisms carrying paternal effects offspring be associated with epigenetic signatures (DNA methylation, histone modification non-coding RNAs), oxidative stress, cytokines, seminal microbiome. Several opportunities exist for sperm/semen influenced during development; these are within testicle, epididymis, or accessory sex glands. Epigenetic sperm impacted pre-natal pre-pubertal periods, sexual maturity advancing sire age. Sperm susceptible alterations as dictated by their developmental stage at time perturbation, plasma likely have both dependent independent on Research using rodent models has revealed many factors including over/under nutrition, dietary fat, protein, ingredient composition (e.g., macro- micronutrients), exercise, exposure drugs, alcohol, endocrine disruptors all elicit responses evident in phenotype. livestock species also age, fertility level, plane heat stress induce epigenetic, cytokine, microbiome profiles and/or plasma. In addition, recent findings pigs, sheep, cattle indicated blastocysts post-fertilization some continuing into post-natal life Our research group focused understanding common management scenarios nutrition growth rates bulls rams subsequent outcomes. Understanding implication imperative short-term feeding decisions potential impact productivity profitability our herds generations come.

Language: Английский

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