Emerging Microbes & Infections,
Journal Year:
2022,
Volume and Issue:
11(1), P. 1281 - 1292
Published: April 19, 2022
Hypervirulent
Klebsiella
pneumoniae
(hvKp)
is
a
notorious
clinical
pathogen
that
more
likely
to
cause
severe
primary
and
metastatic
abscesses.
The
dissemination
of
antimicrobial-resistant
hvKp
isolates
has
been
reported
worldwide,
posing
great
challenge
threat.
However,
the
mechanisms
prevalent
worldwide
are
not
well
precise.
Outer
membrane
vesicles
(OMVs)
secreted
from
gram-negative
bacteria
an
important
vehicle
for
delivering
effector
molecules
inter-
intra-species.
To
explore
whether
OMVs
as
vector
virulence
genes
horizontal
transfer
among
explain
potential
mechanism
development
isolates,
we
isolated
classical
(cKp)
by
sequential
differential
centrifugation,
respectively.
Then,
characteristics
contents
hvKp-OMVs
cKp-OMVs
were
analyzed.
These
contain
genes,
which
could
be
transferred
horizontally
extended-spectrum
beta
lactamase
(ESBL)-producing
cKp,
leading
production
hypervirulent
transformants.
Further
experiments
confirmed
transformants
exhibited
antimicrobial
resistance
phenotypes
in
vitro
vivo.
In
short,
this
work
demonstrated
facilitated
transfer,
allowing
increase
level
ESBL-producing
cKp
providing
new
emergence
isolates.
Virulence,
Journal Year:
2020,
Volume and Issue:
11(1), P. 1215 - 1224
Published: Sept. 12, 2020
Hypervirulent
Klebsiella
pneumoniae(hvKp)
is
an
increasingly
important
pathogen.
Tracking
its
epidemiology
and
evolving
antimicrobial
resistance
will
facilitate
care.
Frontiers in Microbiology,
Journal Year:
2022,
Volume and Issue:
13
Published: Sept. 12, 2022
Carbapenem-resistant
hypervirulent
Klebsiella
pneumoniae
(CR-hvKP),
a
type
of
(KP)
that
exhibits
hypervirulence
and
carbapenem
resistance
phenotypes,
can
cause
severe
infections,
both
hospital-
community-acquired
infections.
CR-hvKP
has
brought
great
challenges
to
global
public
health
is
associated
with
significant
morbidity
mortality.
There
are
many
mechanisms
responsible
for
the
evolution
such
as
horizontal
transfer
plasmid
carrying
gene
(hvKP)
or
carbapenemase-producing
(CRKP)
acquiring
virulence-encoding
gene.
Notably,
KP
evolve
into
by
hybrid
genes.
In
this
review,
we
summarize
evolutionary
plasmid-borne
virulence
well
prevalence
CR-hvKP.
Pathogens and Global Health,
Journal Year:
2022,
Volume and Issue:
117(4), P. 328 - 341
Published: Sept. 11, 2022
For
people
living
in
developed
countries
life
span
is
growing
at
a
faster
pace
than
ever.
One
of
the
main
reasons
for
such
success
attributable
to
introduction
and
extensive
use
clinical
practice
antibiotics
over
course
last
seven
decades.
In
hospital
settings,
Klebsiella
pneumoniae
represents
well-known
commonly
described
opportunistic
pathogen,
typically
characterized
by
resistance
several
antibiotic
classes.
On
other
hand,
broad
wedge
population
Low
and/or
Middle
Income
Countries
increasing
rapidly,
allowing
spread
commensal
bacteria
which
are
transmitted
via
human
contact.
Community
transmission
has
been
original
milieu
K.
isolates
an
outstanding
virulence
(hypervirulent).
These
two
characteristics,
also
defined
as
"pathotypes",
originally
emerged
different
pathways
evolutionary
history
pneumoniae.
long
time,
Sequence
Type
(ST),
combination
alleles
7
housekeeping
genes
Multi-Locus
Typing,
reliable
marker
pathotype:
multidrug-resistant
clones
(e.g.
ST258,
ST147,
ST101)
Western
world
hypervirulent
ST23,
ST65,
ST86)
Eastern.
Currently,
boundaries
separating
pathotypes
fading
away
due
factors,
we
witnessing
worrisome
convergence
certain
high-risk
clones.
Here
review
evidence
available
on
confluence
multidrug-resistance
hypervirulence
specific
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2022,
Volume and Issue:
12
Published: April 27, 2022
Background
Hypervirulent
variants
of
Klebsiella
pneumoniae
(HvKp)
were
typically
associated
with
a
broadly
antimicrobial
susceptible
clone
sequence
type
(ST)
23
at
the
time
its
emergence.
Concerningly,
HvKp
is
now
also
emerging
within
multidrug-resistant
(MDR)
clones,
including
ST11,
ST15,
and
ST147.
MDR-HvKp
either
carry
both
virulence
resistance
plasmids
or
large
hybrid
plasmid
coding
for
determinants.
Here,
we
aimed
to
genetically
characterize
collection
ST2096
isolates
haboring
carrying
(AMR)
genes.
Methods
Nine
K.
isolated
over
1
year
from
blood
sample
hospitalized
patients
in
southern
India
that
MDR
suspected
be
selected.
All
nine
subjected
short-read
whole-genome
sequencing;
subset
(n
=
4)
was
additionally
long-read
sequencing
obtain
complete
genomes
characterization.
Mucoviscosity
assay
performed
phenotypic
assessment.
Results
Among
isolates,
seven
carbapenem-resistant,
two
which
carried
bla
NDM-5
on
an
IncFII
five
OXA-232
ColKP3
plasmid.
The
organisms
confirmed
as
HvKp,
characteristic
genes
(
rmpA2
,
iutA
iucABCD
)
(~320
kbp)
IncFIB–IncHI1B
co-integrate.
This
aadA2
armA
OXA-1
msrE
mphE
sul1
dfrA14
AMR
addition
heavy-metal
showed
about
60%
similarity
IncHI1B
SGH10
~70%
identity
first
identified
pNDM-MAR
Notably,
IV-A3
CRISPR-Cas
system
harbored
spacer
regions
against
traL
IncF
plasmids,
thereby
preventing
their
acquisition.
Conclusion
convergence
clinically
concerning
.
Our
data
highlight
role
ST2096,
suggesting
not
confined
selected
clones;
continued
emergence
such
genotypes
across
species.
occurring
globally
amidst
several
clones
great
concern
public
health.
Antimicrobial Agents and Chemotherapy,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Feb. 28, 2023
Increasing
occurrence
of
multidrug-resistant
(MDR)
and
hypervirulent
(hv)
Klebsiella
pneumoniae
(MDR-hvKp)
convergent
clones
is
being
observed.
Those
strains
have
the
potential
causing
difficult-to-treat
infections
in
healthy
adults
with
an
increased
capacity
for
mortality.
Microbiology Spectrum,
Journal Year:
2023,
Volume and Issue:
11(1)
Published: Jan. 9, 2023
Chromosomal
recombination
events
are
considered
to
contribute
the
genetic
diversification
and
ultimate
success
of
many
bacterial
pathogens.
A
previous
study
unravelled
molecular
evolution
history
ST258
strains,
which
have
been
largely
responsible
for
spread
KPC
in
United
States.
Genome Medicine,
Journal Year:
2023,
Volume and Issue:
15(1)
Published: Dec. 1, 2023
Abstract
Background
Klebsiella
pneumoniae
is
a
notorious
clinical
pathogen
and
frequently
carries
various
plasmids,
which
are
the
main
carriers
of
antimicrobial
resistance
virulence
genes.
In
comparison
to
self-transmissible
conjugative
mobilizable
plasmids
have
received
much
less
attention
due
their
defects
in
elements.
However,
contribution
horizontal
transfer
genes
K.
remains
unclear.
this
study,
transfer,
stability,
cargo
were
examined
via
genetic
experiments
genomic
analysis
.
Methods
Carbapenem-resistant
(CR)
plasmid
pHSKP2
multidrug-resistant
(MDR)
pHSKP3
HS11286,
pRJF293
RJF293
employed
conjugation
assays
assess
ability
plasmids.
Mimic
genetically
modified
constructed
confirm
cotransfer
models.
The
morphology
was
evaluated
through
Xba
I
S1
nuclease
pulsed-field
gel
electrophoresis
and/or
complete
genome
sequencing.
Mobilizable
stability
transconjugants
analyzed
serial
passage
culture.
addition,
silico
3923
1194
completely
sequenced
performed
investigate
distribution
elements,
genes,
targets
CRISPR-Cas
system.
MDR
investigated,
carry
oriT
but
lack
other
Results
Our
results
showed
that
carrying
lacking
relaxase
gene
able
with
helper
CR
across
Escherichia
coli
strains.
rather
than
not
interfered
by
CRISPR–Cas
system
recipient
According
analysis,
about
twenty
percent
acquired
more
seventy-five
pneumoniae.
Conclusions
work
observed
or
lacks
transferred
escaped
from
defence
remained
stable
recipients.
These
highlight
threats
as
vital
vehicles
dissemination
antibiotic
Microbiology Spectrum,
Journal Year:
2023,
Volume and Issue:
11(2)
Published: Feb. 8, 2023
Klebsiella
pneumoniae
is
a
leading
cause
of
sepsis
in
newborns
and
adults.
Among
the
2
major
pathotypes
K.
,
classical
(cKP)
hypervirulent
(hvKP),
hvKP
causes
community-acquired
severe
fatal
invasive
infections
even
healthy
individuals,
as
it
possesses
several
virulence
factors.
