Hydrogels loaded with MSC‐derived small extracellular vesicles: A novel cell‐free tissue engineering system for diabetic wound management

View, Journal Year: 2024, Volume and Issue: 5(4)

Published: July 18, 2024

Abstract With the aging and obesity era, increasing incidence of diabetes diabetic complications, especially non‐healing wounds, imposes a serious economic burden on both patients society. The complex microenvironments, including hyperglycemia, bacterial infection, ischemia, nerve damage, lead to prolonged inflammation proliferation phase wounds. Mesenchymal stem cell‐derived small extracellular vesicles (MSC‐sEVs), which contain rich variety therapeutic molecules, have been chased for decades because their potential roles in cellular communication, tissue regeneration, drug delivery. As powerful tools controlled‐sustained release sEVs, biocompatible hydrogels applied wide range biomedical applications. Herein, we first summarize pathological features such as angiopathy, neuropathy, immune cell dysfunction. Then, discuss biological properties, performance, stability pure MSC‐sEVs. After that, components, application patterns, responsiveness hydrogels. Next, loading avenues MSC‐sEVs into hydrogel, behaviors sEVs from hydrogels, influence crosslinking method hydrogel‐sEV composites. Finally, provide an overview current applications loaded with novel cell‐free engineering system managing wounds propose critical unsolved issues. This review is expected meaningful guidance developing wound management.

Language: Английский

The Rejuvenation and Functional Restoration of Aged Adipose Stem Cells by DUXAP10 Knockdown via the Regulation of the miR-214-3p/RASSF5 Axis

Stem Cells Translational Medicine, Journal Year: 2024, Volume and Issue: 13(5), P. 462 - 476

Published: March 9, 2024

Abstract Adipose stem cell (ASC)-based therapies provide an encouraging option for tissue repair and regeneration. However, the function of these cells declines with aging, which limits their clinical transformation. Recent studies have outlined involvement long non-coding RNAs in aging. Here, we reanalyzed our published RNA sequencing (RNA-seq) data profiling differences between ASCs from young old donors identified a lncRNA named double homeobox A pseudogene 10 (DUXAP10) as significantly accumulated aged ASCs. Knocking down DUXAP10 promoted proliferation migration halted senescence secretion proinflammatory cytokines. In addition, was located cytoplasm functioned decoy miR-214-3p. miR-214-3p downregulated ASCs, its overexpression rejuvenated reversed harm caused by DUXAP10. Furthermore, Ras Association Domain Family Member 5 (RASSF5) target upregulated Overexpressing inhibiting both enhanced RASSF5 content while knockdown therapeutic ability skin wound healing. Overall, this study offers new insights into mechanism age-related ASC dysfunction names potential targets energizing cells.

Language: Английский