A dataset of digital holograms of normal and thalassemic cells

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Jan. 2, 2024

Digital holographic microscopy (DHM) is an intriguing medical diagnostic tool due to its label-free and quantitative nature, providing high-contrast images of phase samples. By capturing both intensity information, DHM enables the numerical reconstruction images. However, lateral resolution limited by diffraction limit, which prompted recent suggestion microsphere-assisted enhance straightforwardly. The use such a technique as requires testing validation proposed assays prove their feasibility viability. This paper publishes 760 609 normal thalassemic red blood cells obtained from male individual, respectively.

Language: Английский

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In-situ detection of microplastics in the aquatic environment: A systematic literature review

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 934, P. 173111 - 173111

Published: May 12, 2024

Microplastics are ubiquitous in the aquatic environment and have emerged as a significant environmental issue due to their potential impacts on human health ecosystem. Current laboratory-based microplastic detection methods suffer from various drawbacks, including lack of standardisation, limited spatial temporal coverage, high costs, time-consuming procedures. Consequently, there is need for development in-situ techniques detect monitor microplastics effectively identify understand sources, pathways, behaviours. Herein, we adopt systematic literature review method assess application experimental field technologies designed monitoring microplastics, without sample preparation. Four scientific databases were searched March 2023, resulting 62 relevant studies. These studies classified into seven sensor categories working principles discussed. The classes include optical devices, digital holography, Raman spectroscopy, other hyperspectral imaging, remote sensing, methods. We also looked at how data these integrated with machine learning models develop classifiers capable accurately characterising physical chemical properties discriminating them particles. This concluded that environments feasible can be achieved accuracy, even though still early stages development. Nonetheless, further research needed enhance microplastics. includes exploring possibility combining developing robust machine-learning classifiers. Additionally, recommendation implementation reviewed effectiveness detecting limitations.

Language: Английский

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Single-shot self-supervised object detection in microscopy

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Dec. 5, 2022

Language: Английский

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A Tutorial on Immersive Video Delivery: From Omnidirectional Video to Holography

IEEE Communications Surveys & Tutorials, Journal Year: 2023, Volume and Issue: 25(2), P. 1336 - 1375

Published: Jan. 1, 2023

Video services are evolving from traditional two-dimensional video to virtual reality and holograms, which offer six degrees of freedom users, enabling them freely move around in a scene change focus as desired. However, this increase translates into stringent requirements terms ultra-high bandwidth (in the order Gigabits per second) minimal latency milliseconds). To realize such immersive services, network transport, well representation encoding, have be fundamentally enhanced. The purpose tutorial article is provide an elaborate introduction creation, streaming, evaluation video. Moreover, it aims lessons learned point at promising research paths enable truly interactive applications toward holography.

Language: Английский

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Holoreact: chemistry experiment game with hologram based to enhance learning on senior high school level

Procedia Computer Science, Journal Year: 2023, Volume and Issue: 216, P. 453 - 461

Published: Jan. 1, 2023

The survey conducted on 104 students from 14 schools through online questionnaires found that considered chemistry as a complex subject. This is also supported by the declining score result chemistry's National Examination, specifically in analytic section for past three years. Based survey, were interested learning an experiment hologram-based game because it more immersive and easier to observe. Hence made using ELEKTRA method, which aims teach high-school concepts chemistry. test showed increase of understanding 47% - 62% both pre post-experiment quiz scores. expert's interview result, game's content has been compatible with current high school curriculum.

Language: Английский

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Transport of intensity and phase: applications to digital holography [Invited]

Applied Optics, Journal Year: 2024, Volume and Issue: 63(10), P. 2436 - 2436

Published: March 1, 2024

We first review transport of intensity and phase show their use as a convenient tool to directly determine the unwrapped an imaged object, either through conventional imaging or using digital holography. For both cases, traditional phase, with modification, viz., electrically controllable can be used. The holography for 3D topographic mapping fingermarks coated columnar thin films is shown illustrative application this versatile technique.

Language: Английский

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Single-shot wavelength-multiplexed digital holography for 3D fluorescent microscopy and other imaging modalities

Applied Physics Letters, Journal Year: 2020, Volume and Issue: 117(3)

Published: July 20, 2020

We propose a holographic technique by which multiwavelength three-dimensional spatial information is obtained with both single-shot exposure of monochrome image sensor and an in-line multiplexing scheme. develop specially designed wavelength-dependent phase-modulation array, used to obtain the required for proposed technique. The applicable not only laser holography but also spatially temporally incoherent holography. Its validity experimentally demonstrated constructing color-multiplexed fluorescence digital microscope multicolor system white-light source.

Language: Английский

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Single-shot common-path off-axis digital holography: applications in bioimaging and optical metrology [Invited]

Applied Optics, Journal Year: 2020, Volume and Issue: 60(4), P. A195 - A195

Published: Oct. 15, 2020

The demand for single-shot and common-path holographic systems has become increasingly important in recent years, as such offer various advantages compared to their counterparts. Single-shot systems, example, reduce computational complexity only a single hologram with the object information required process, making them more suitable investigation of dynamic events; are less vibration-sensitive, compact, inexpensive, high temporal phase stability. We have developed off-axis digital setup based on beam splitter pinhole. In this paper, we present concise review proposed system several applications, including quantitative imaging investigate morphological parameters, metrological tool testing micro-optics, industrial inspection measurement, sound field visualization.

Language: Английский

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Holography applications toward medical field: An overview

Indian journal of radiology and imaging - new series/Indian journal of radiology and imaging/Indian Journal of Radiology & Imaging, Journal Year: 2020, Volume and Issue: 30(3), P. 354 - 354

Published: Jan. 1, 2020

3D Holography is a commercially available, disruptive innovation, which can be customised as per the requirements and supporting Industry 4.0. The purpose of this paper to study potential applications holography in medical field. This explores concept its significant benefits field.The derived through various research papers on tries identify direction &development see how innovative technology used effectively for better treatment patients.Holography uses digital imaging inputs provides an extensive visualisation data training doctors, surgeons students. converts information about body into format has inform, promote entertain students doctors. However, it needs large amount space storage software support analysis skills customising. seems good solve variety issues by storing using patient developing holograms, are useful assist successful surgery. It providing flexible solutions area research. Finally, identifies 13 field discusses them appropriately.The holographic due capability image processing. Holographic images non-contact having depth. A physician now zoom view part. create advancements diagnosis process, improve practice. helps quick detection problems organs like brain, heart, liver, kidney etc. By technology, practitioners colourful at multiple angles with accuracy. opens up way planning, testing procedures diagnosis. With technological developments, compact hardware available help related applications.

Language: Английский

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Combining multi-marker metabarcoding and digital holography to describe eukaryotic plankton across the Newfoundland Shelf

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: July 29, 2022

The planktonic diversity throughout the oceans is vital to ecosystem functioning and linked environmental change. Plankton monitoring tools have advanced considerably with high-throughput in-situ digital cameras genomic sequencing, opening new challenges for high-frequency observations of community composition, structure, species discovery. Here, we combine multi-marker metabarcoding based on nuclear 18S (V4) plastidial 16S (V4-V5) rRNA gene amplicons a in-line holographic microscope provide synoptic survey eukaryotic plankton along Newfoundland Shelf (Canada) during winter transition phase North Atlantic bloom phenomenon. Metabarcoding revealed rich unidentifiable in imaging samples, confirming presence ecologically important saprophytic protists which were unclassifiable matching images, detecting groups unobserved or taxonomically unresolved similar sequencing campaigns Northwest Ocean. In turn, analysis provided quantitative widely prevalent from every trophic level. Despite contrasting compositions portrayed by each sampling method, both capture broad spatial differences between northern southern sectors suggest complementary estimations features assemblages. Future tasks will involve standardizing wider use consistent, comparable ocean observations.

Language: Английский

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