New Insights on the Role of ß-Cyanoalanine Synthase CAS-C1 in Root Hair Elongation through Single-Cell Proteomics DOI Creative Commons
Lucía Arenas-Alfonseca, Masashi Yamada, Luís C. Romero

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(23), P. 4055 - 4055

Published: Dec. 2, 2023

(1) Background: Root hairs are specialized structures involved in water and plant nutrient uptake. They elongate from epidermal cells following a complex developmental program. ß-cyanoalanine synthase (CAS), which is mainly hydrogen cyanide (HCN) detoxification Arabidopsis thaliana, plays role root hair elongation, as evidenced by the fact that cas-c1 mutants show severe defect shape. In addition to hairs, CAS C1 expressed quiescent center meristem. (2) Methods: To identify its formation, we conducted single-cell proteomics analysis isolating using Fluorescence-activated Cell Sorting (FACS) wild-type mutants. We also analyzed presence of S-cyanylation, protein post-translational modification (PTM) mediated HCN affecting cysteine residues activity proteins wild type (3) Results Conclusions: have found mutation has no visible effect on or meristem tissue, both control nutrient-deprivation conditions. identified more than 3900 several development, related receptor kinase FERONIA signaling DNA methylation, modified S-cyanylation.

Language: Английский

Plant Cyanogenic-Derived Metabolites and Herbivore Counter-Defences DOI Creative Commons
Manuel Martínez, Isabel Dı́az

Plants, Journal Year: 2024, Volume and Issue: 13(9), P. 1239 - 1239

Published: April 29, 2024

The release of cyanide from cyanogenic precursors is the central core plant defences based on cyanogenesis process. Although formed as a coproduct some metabolic routes, its production mostly due to degradation cyanohydrins originating glycosides in plants and 4-OH-ICN route Brassicaceae. Cyanohydrins are then hydrolysed reversible reaction generating cyanide, being both, toxic compounds with potential defensive properties against pests pathogens. Based cyanogenic-derived molecules response damage caused by herbivore infestation, this review, we compile actual knowledge events plant–pest context. Besides potential, mode action, targets combat phytophagous insects acari, special attention has been paid arthropod responses strategies overcome impact cyanogenesis. Physiological behavioural adaptations, well detoxification β-cyanoalanine synthases, rhodaneses, cyanases common ways arthropods produced plants. Much experimental work needed further understand complexities specificities defence–counter-defence system be applied breeding programs.

Language: Английский

Citations

8
Regulation of mammalian cellular metabolism by endogenous cyanide production DOI Creative Commons
Karim Zuhra, Maria Petrosino, Lucia Janickova

et al.

Nature Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Abstract Small, gaseous molecules such as nitric oxide, carbon monoxide and hydrogen sulfide are produced signalling in mammalian cells. Here, we show that low concentrations of cyanide generated endogenously various tissues We detect several cellular compartments human cells the blood mice. Cyanide production is stimulated by glycine, occurs at pH lysosomes requires peroxidase activity. When a specific rate, exerts stimulatory effects on mitochondrial bioenergetics, cell metabolism proliferation, but impairs bioenergetics high concentrations. can modify cysteine residues via protein S -cyanylation, which detectable basally mice, increases response to glycine. Low-dose supplementation exhibits cytoprotective hypoxia reoxygenation models vitro vivo. Conversely, pathologically elevated nonketotic hyperglycinaemia detrimental Our findings indicate should be considered part same group endogenous regulatory gasotransmitters sulfide.

Language: Английский

Citations

1
Aluminum-doped T-graphene: An innovative platform for enhanced sensitivity in hydrogen cyanide detection DOI
Mounir M. Salem‐Bekhit, Mustafa Mudhafar,

Zainab Ahmed Abass

et al.

Chemical Physics, Journal Year: 2025, Volume and Issue: unknown, P. 112648 - 112648

Published: Feb. 1, 2025

Language: Английский

Citations

0
Proteomic and Functional Analysis of Hydrogen Cyanide-Mediated Defense and Primed Response to Pseudomonas in Arabidopsis DOI
Luís C. Romero, P. Díaz-Rueda,

Carlos P. Sosa

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Gases define redox signalling: NO, H2S, O2 … and cyanide DOI
Zachary W. Grimmett, Joseph C. Schindler, Jonathan S. Stamler

et al.

Nature Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Language: Английский

Citations

0
The application and development of cyanating reagents in asymmetric cyanation reactions DOI
Ruirui Hua, Chukai Shao, Fu‐Xue Chen

et al.

Tetrahedron Letters, Journal Year: 2025, Volume and Issue: unknown, P. 155549 - 155549

Published: March 1, 2025

Language: Английский

Citations

0
Sensitivity and responses of sorghum hybrid lines to wheat aphid (Sitobion avenae Fabricius) infestation DOI

Ivelina Nikolova

Annals of Applied Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Abstract Sorghum bicolor (L.) Moench is a resilient cereal crop that thrives in arid conditions and produces substantial yields following periods of drought. However, sorghum productivity threatened by insect pests, such as Sitobion avenae Fabricius, common aphid species on sorghum. The research included seven hybrid lines between 2021 2023. study aimed to evaluate the cyanogenic glycoside content these lines, their sensitivity infestation, effect S. attack integrity plastid pigments alteration leaf chlorophyll fluorescence parameters. Aphid infestation occurred under natural field conditions. revealed classified very low‐sensitive hybrids (L №305 Whiteseed) exhibited significantly lower density , attributable substantially more elevated concentration glycosides non‐infested plants significant decrease infested plants. Changes carotenoid contents parameters showed slight reduction. This led stronger protective antibiotic host weaker performance. medium‐sensitive №302 L №256) higher because considerably violent hybrids. resulted considerable reduction levels pronounced photosynthetic sensitive plants' stress response invasion. study's findings enhance understanding how impact essential for identifying selecting resistant varieties. These traits are pivotal determining yield, thus serving crucial indicators future endeavours concerning resistance

Language: Английский

Citations

0
Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco DOI Creative Commons

Luhua Yang,

Yuan Guo, Hui Yang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 1, 2024

The plant microbiota is believed to be an accessory genome that extends functions, forming holobionts together with the host plant. Plant disease resistance, therefore, inextricably linked microbiota, which play important roles in growth and health. To explore relationship between we investigated tobacco microbiome of two varieties contrasting disease-resistance levels bacterial wilt black shank diseases. Comparative analysis indicated resistant variety assembled a distinct higher network complexity diversity. While Pseudomonas Ensifer, contain biocontrol beneficial members, were enriched rhizosphere variety, Ralstonia , genus including known causative pathogen, was susceptible variety. Metagenome sequencing revealed such as hydrogen cyanide synthase, pyochelin biosynthesis, arthrofactin-type cyclic lipopeptide synthetase, more abundant Further contigs encoding corresponding genes mostly assigned . Among all metagenome-assembled genomes, positive selection suggested only search biosynthetic gene clusters non-ribosomal peptide compound brabantamide A, antimicrobial activity. Collectively, our study suggests might involved microbe-mediated resistance. Particularly, results highlight disease-resistant promising candidate. Our may facilitate further screening isolates targeted design microbial communities.

Language: Английский

Citations

3
The case for sporadic cyanogenic glycoside evolution in plants DOI Creative Commons
Raquel Sánchez‐Pérez, Elizabeth Heather Jakobsen Neilson

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 81, P. 102608 - 102608

Published: July 31, 2024

Cyanogenic glycosides are α-hydroxynitrile glucosides present in approximately 3000 different plant species. Upon tissue disruption, cyanogenic hydrolyzed to release toxic hydrogen cyanide as a means of chemical defense. Over 100 have been reported, with structural diversity dependent on the precursor amino acid, and subsequent modifications. represent prime example sporadic metabolite evolution, metabolic trait arising multiple times throughout lineage evidenced by recruitment enzyme families for biosynthesis. Here, we review latest developments within glycoside biosynthesis, argue possible factors driving evolution including shared intermediates crossovers other pathways crossovers, multifunctionality beyond

Language: Английский

Citations

2
Germination of 10 midland plant species from the eastern Mediterranean Basin: effects of smoke, syringaldehyde, karrikinolide and cyanohydrin DOI
Şükrü Serter Çatav, Kenan Akbaş, Yaren Fulya Kaplan

et al.

Nordic Journal of Botany, Journal Year: 2024, Volume and Issue: 2024(10)

Published: July 26, 2024

The beneficial effects of smoke and its constituents, karrikinolide (KAR 1 ) cyanohydrin glyceronitrile, on the germination Mediterranean lowland species are well‐documented. However, very little is known about role these signals plants at higher altitudes. In addition, lignin‐derived chemicals, such as syringaldehyde (SAL), have recently been proposed overlooked cues for smoke‐induced germination. To address gaps in literature, we investigated smoke‐water SAL 10 midland growing serpentine soils. We also sought to determine whether interacts with KAR and/or mandelonitrile (MAN, a cyanohydrin) enhance seed results show that significantly improved three ( Barbarea duralii , Digitalis cariensis Turritis laxa ). SAL, other hand, had no positive effect tested. Furthermore, smoke‐sensitive Verbascum cariense responded MAN. Finally, did not exert any synergistic interaction conclusion, provide evidence an important cue species. Moreover, play stimulating species, either independently or combination chemicals.

Language: Английский

Citations

1