Assessment of the safety and probiotic properties of Roseburia intestinalis: A potential “Next Generation Probiotic” DOI Creative Commons
Chao Zhang,

Kejia Ma,

Kai Nie

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 8, 2022

Roseburia intestinalis is an anaerobic bacterium that produces butyric acid and belongs to the phylum Firmicutes. There increasing evidence this has positive effects on several diseases, including inflammatory bowel disease, atherosclerosis, alcoholic fatty liver, colorectal cancer, metabolic syndrome, making it a potential “Next Generation Probiotic.” We investigated genomic characteristics, probiotic properties, cytotoxicity, oral toxicity, colonization characteristics of bacterium, its effect gut microbiota. The genome contains few genes encoding virulence factors, three clustered regularly interspaced short palindromic repeat (CRISPR) sequences, two Cas genes, no toxic biogenic amine synthesis essential amino vitamin genes. Seven prophages 41 islands were predicted. In addition bacteriocin (Zoocin A), encodes four gene clusters synthesize short-chain acids 222 carbohydrate-active enzyme modules. This sensitive antibiotics specified by European Food Safety Authority, does not exhibit hemolytic or gelatinase activity, exhibits some resistance. R. adheres intestinal epithelial cells inhibits invasion certain pathogens. vitro experiments showed was cytotoxic. did affect diversity abundance flora. Using fluorescent labelling method, we discovered colonizes cecum mucus colon. An toxicity study reveal any obvious adverse effects. lethal dose (LD)50 exceeded 1.9 × 10 9 colony forming units (CFU)/kg, whereas observed level (NOAEL) derived from 1.32 CFU/kg/day for 28 days. current research shows that, suitable next-generation considering properties safety.

Language: Английский

Toward a Deeper Understanding of Gut Microbiome in Depression: The Promise of Clinical Applicability DOI Creative Commons
Lanxiang Liu, Haiyang Wang,

Hanping Zhang

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(35)

Published: Oct. 26, 2022

The emergence of the coronavirus disease 2019 pandemic has dramatically increased global prevalence depression. Unfortunately, antidepressant drugs benefit only a small minority patients. Thus, there is an urgent need to develop new interventions. Accumulating evidence supports causal relationship between gut microbiota dysbiosis and To advance microbiota-based diagnostics therapeutics depression, comprehensive overview microbial alterations in depression presented identify effector biomarkers. This procedure generated 215 bacterial taxa from humans 312 animal models. Compared controls, shows significant differences β-diversity, but no changes richness diversity. Additionally, species-specific are identified like Eggerthella decreased Acetatifactor rodent Moreover, disrupted microbiome balance functional changes, characterized by enrichment pro-inflammatory bacteria (e.g., Desulfovibrio Escherichia/Shigella) depletion anti-inflammatory butyrate-producing Bifidobacterium Faecalibacterium) consistently shared across species. Confounding effects geographical region, type, intestinal segments also investigated. Ultimately, total 178 species subspecies probiotics alleviate depressive phenotypes. Current findings provide foundation for developing advancing microbiota-oriented precision medicine

Language: Английский

Citations

89
Gut microbial metabolite butyrate improves anticancer therapy by regulating intracellular calcium homeostasis DOI
Yibin Che, Guoyu Chen, Qianqian Guo

et al.

Hepatology, Journal Year: 2023, Volume and Issue: 78(1), P. 88 - 102

Published: Jan. 3, 2023

Gut microbiota are recognized to be important for anticancer therapy, yet the underlying mechanism is not clear. Here, through analysis of clinical samples, we identify by which gut microbial metabolite butyrate inhibits HCC and then explore new strategies treatment.In our study, demonstrate that improves therapy efficacy regulating intracellular calcium homeostasis. Using liquid chromatography-mass spectrometry analysis, found metabolism activated in patients compared with healthy individuals. Butyrate levels lower plasma gas (GC-MS) analysis. supplementation or depletion short-chain Acyl-CoA dehydrogenase (SCAD) gene (ACADS), encoding a key enzyme metabolism, significantly proliferation metastasis. The profiling genes upregulated ACADS knockdown reveals signaling pathway activated, leading dysregulation homeostasis production reactive oxygen species. tyrosine kinase inhibitor sorafenib. On basis these findings, developed sorafenib coencapsulated mPEG-PLGA-PLL nanoparticles coated anti-GPC3 antibody (BS@PEAL-GPC3) prolong retention time drugs enhance drug targeting, high efficacy. BS@PEAL-GPC3 reduce progression. In addition, display excellent targeting safety.In conclusion, findings provide insight into metabolites inhibit progression, suggesting translatable therapeutics approach targeted therapeutic

Language: Английский

Citations

46
Oncomicrobial Community Profiling Identifies Clinicomolecular and Prognostic Subtypes of Colorectal Cancer DOI
Dmitri Mouradov, Paul Greenfield, Shan Li

et al.

Gastroenterology, Journal Year: 2023, Volume and Issue: 165(1), P. 104 - 120

Published: March 16, 2023

Language: Английский

Citations

45
Robust reactive oxygen species modulator hitchhiking yeast microcapsules for colitis alleviation by trilogically intestinal microenvironment renovation DOI Creative Commons

Jin-Tao Li,

Jian Song, Zhichao Deng

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 36, P. 203 - 220

Published: March 5, 2024

Ulcerative colitis (UC) is characterized by chronic inflammatory processes of the intestinal tract unknown origin. Current treatments lack understanding on how to effectively alleviate oxidative stress, relieve inflammation, as well modulate gut microbiota for maintaining homeostasis synchronously. In this study, a novel drug delivery system based metal polyphenol network (MPN) was constructed via coordination between epigallocatechin gallate (EGCG) and Fe

Language: Английский

Citations

28
Application of next-generation sequencing to identify different pathogens DOI Creative Commons

Aljuboori M. Nafea,

Yuer Wang,

Duanyang Wang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 29, 2024

Early and precise detection identification of various pathogens are essential for epidemiological monitoring, disease management, reducing the prevalence clinical infectious diseases. Traditional pathogen techniques, which include mass spectrometry, biochemical tests, molecular testing, culture-based methods, limited in application time-consuming. Next generation sequencing (NGS) has emerged as an technology identifying pathogens. NGS is a cutting-edge method with high throughput that can create massive volumes sequences broad prospects field diagnosis. In this review, we introduce detail, summarizes different pathogens, including bacteria, fungi, viruses, analyze challenges outlook using to identify Thus, work provides theoretical basis studies evidence support distinguishing

Language: Английский

Citations

25
Engineered probiotic ameliorates ulcerative colitis by restoring gut microbiota and redox homeostasis DOI
Peilin Guo, Wenjing Wang, Qian Xiang

et al.

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(9), P. 1502 - 1518.e9

Published: Aug. 27, 2024

Language: Английский

Citations

19
Integration of 168,000 samples reveals global patterns of the human gut microbiome DOI Creative Commons
Richard J. Abdill, Samantha P. Graham, Vincent Rubinetti

et al.

Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The factors shaping human microbiome variation are a major focus of biomedical research. While other fields have used large sequencing compendia to extract insights requiring otherwise impractical sample sizes, the field has lacked comparably sized resource for 16S rRNA gene amplicon commonly quantify composition. To address this gap, we processed 168,464 publicly available gut samples with uniform pipeline. We use compendium evaluate geographic and technical effects on variation. find that regions such as Central Southern Asia differ significantly from more thoroughly characterized microbiomes Europe Northern America composition alone can be predict sample's region origin. also strong associations between primers DNA extraction. anticipate growing work, Human Microbiome Compendium, will enable advanced applied methodological

Language: Английский

Citations

3
SPIRE: a Searchable, Planetary-scale mIcrobiome REsource DOI Creative Commons
Thomas Schmidt, Anthony Fullam, Pamela Ferretti

et al.

Nucleic Acids Research, Journal Year: 2023, Volume and Issue: 52(D1), P. D777 - D783

Published: Oct. 28, 2023

Abstract Meta’omic data on microbial diversity and function accrue exponentially in public repositories, but derived information is often siloed according to type, study or sampled environment. Here we present SPIRE, a Searchable Planetary-scale mIcrobiome REsource that integrates various consistently processed metagenome-derived modalities across habitats, geography phylogeny. SPIRE encompasses 99 146 metagenomic samples from 739 studies covering wide array of environments augmented with manually-curated contextual data. Across total assembly 16 Tbp, comprises 35 billion predicted protein sequences 1.16 million newly constructed metagenome-assembled genomes (MAGs) medium high quality. Beyond mapping the high-quality genome reference provided by proGenomes3 (http://progenomes.embl.de), these novel MAGs form 92 134 species-level clusters, majority which are unclassified at species level using current tools. enables taxonomic profiling clusters via an updated, custom mOTUs database (https://motu-tool.org/) includes several layers functional annotation, as well crosslinks (micro-)biological databases. The resource accessible, searchable browsable http://spire.embl.de.

Language: Английский

Citations

28
Inactivation of the antidiabetic drug acarbose by human intestinal microbial-mediated degradation DOI
Jinzhong Tian, Chong Li,

Zhixiang Dong

et al.

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(5), P. 896 - 909

Published: May 8, 2023

Language: Английский

Citations

27
Effects of microbial-derived biotics (meta/pharma/post-biotics) on the modulation of gut microbiome and metabolome; general aspects and emerging trends DOI
Alireza Sadeghi, Maryam Ebrahimi, Mohammad Saeed Kharazmi

et al.

Food Chemistry, Journal Year: 2023, Volume and Issue: 411, P. 135478 - 135478

Published: Jan. 12, 2023

Language: Английский

Citations

26