Plant organellar RNA maturation

The Plant Cell, Journal Year: 2023, Volume and Issue: 35(6), P. 1727 - 1751

Published: Feb. 21, 2023

Abstract Plant organellar RNA metabolism is run by a multitude of nucleus-encoded RNA-binding proteins (RBPs) that control stability, processing, and degradation. In chloroplasts mitochondria, these post-transcriptional processes are vital for the production small number essential components photosynthetic respiratory machinery—and consequently biogenesis plant survival. Many RBPs have been functionally assigned to individual steps in maturation, often specific selected transcripts. While catalog factors identified ever-growing, our knowledge how they achieve their functions mechanistically far from complete. This review summarizes current taking an RBP-centric approach focusing on mechanistic aspects RBP kinetics involved in.

Language: Английский

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Phase separation of GRP7 facilitated by FERONIA-mediated phosphorylation inhibits mRNA translation to modulate plant temperature resilience

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(3), P. 460 - 477

Published: Feb. 6, 2024

Language: Английский

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Mapping protein–RNA binding in plants with individual-nucleotide-resolution UV cross-linking and immunoprecipitation (plant iCLIP2)

Nature Protocols, Journal Year: 2024, Volume and Issue: 19(4), P. 1183 - 1234

Published: Jan. 26, 2024

Language: Английский

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RNA-binding proteins that preferentially interact with 8-oxoG-modified RNAs: our current understanding

Biochemical Society Transactions, Journal Year: 2024, Volume and Issue: 52(1), P. 111 - 122

Published: Jan. 4, 2024

Cells encounter a variety of stresses throughout their lifetimes. Oxidative stress can occur via myriad factors, including exposure to chemical toxins or UV light. Importantly, these stressors induce changes (e.g. modifications) biomolecules, such as RNA. Commonly, guanine is oxidized form 8-oxo-7,8-hydroxyguanine (8-oxoG) and this modification disrupt plethora cellular processes messenger RNA translation stability. Polynucleotide phosphorylase (PNPase), heterogeneous nuclear ribonucleoprotein D (HNRPD/Auf1), poly(C)-binding protein (PCBP1/HNRNP E1), Y-box binding 1 (YB-1) have been identified four RNA-binding proteins that preferentially bind 8-oxoG-modified over unmodified All are native humans PNPase additionally found in bacteria. Additionally, under oxidative stress, cell survival declines mutants lack PNPase, Auf1, PCBP1, suggesting they critical the response. This mini-review captures current understanding HNRPD/Auf1, YB-1 mechanism has outlined so far by which recognize interact with RNAs.

Language: Английский

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The Glycine-Rich RNA-Binding Protein Is a Vital Post-Transcriptional Regulator in Crops

Plants, Journal Year: 2023, Volume and Issue: 12(19), P. 3504 - 3504

Published: Oct. 9, 2023

Glycine-rich RNA binding proteins (GR-RBPs), a branch of (RBPs), play integral roles in regulating various aspects metabolism regulation, such as processing, transport, localization, translation, and stability, ultimately regulate gene expression cell fate. However, our current understanding GR-RBPs has predominantly been centered on Arabidopsis thaliana, model plant for investigating growth development. Nonetheless, an increasing body literature emerged recent years, shedding light the presence functions GRPs diverse crop species. In this review, we not only delineate distinctive structural domains but also elucidate several contemporary mechanisms post-transcriptional regulation RNA. These encompass intricate processes, including alternative splicing, polyadenylation, miRNA biogenesis, phase separation, translation. Furthermore, offer exhaustive synthesis that fulfill within plants. Our overarching objective is to provide researchers practitioners field agricultural genetics with valuable insights may inform guide application genetic engineering enhanced development sustainable agriculture.

Language: Английский

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Priming the pump: translational dynamics from seed to seedling transition under priming treatment

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Language: Английский

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Unveiling the regulatory role of GRP7 in ABA signal-mediated mRNA translation efficiency regulation

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 26, 2025

Abscisic acid (ABA) is a crucial phytohormone involved in plant growth and stress responses. While the transcriptional regulation triggered by ABA well-documented, its effects on translational have been less studied. Through Ribo-seq RNA-seq analyses, we find that treatment not only influences gene expression at mRNA level but also significantly impacts translation efficiency (TE) Arabidopsis thaliana. inhibits global via core signaling pathway, which includes receptors, protein phosphatase 2Cs (PP2Cs), SNF1-related kinase 2 s (SnRK2s). Upon treatment, Glycine-rich RNA-binding proteins 7 8 (GRP7&8) levels decrease due to both reduced decreased TE, diminishes their association with polysomes leads decline TE. The absence of GRP7&8 results impairment ABA-regulated changes, linking GRP7-dependent modulation translation. GRP7 TE relies direct binding target mRNAs. Moreover, under drought partially dependent ABA-GRP7&8 pathways. Collectively, our study reveals GRP7's role downstream SnRK2s mediating signaling, offering model for ABA-triggered multi-route environmental adaptation.

Language: Английский

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The Regulatory Roles of RNA-Binding Proteins in Plant Salt Stress Response

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1402 - 1402

Published: May 7, 2025

Salt stress is one of the most prominent abiotic stresses. Behind intricate adaptive responses plants to salt stress, regulation gene expression assumes a pivotal role. Complementing transcriptional mechanisms, post-transcriptional performed by RNA-binding proteins provides an additional layer control through sophisticated molecular machinery. RBPs interact with both RNA molecules and protein partners coordinate metabolism and, thus, fine-tune salt-responsive genes, enabling rapidly adapt ionic challenges. This review systematically evaluates functional roles localized in distinct subcellular compartments, including nuclear, cytoplasmic, chloroplastic, mitochondrial systems, mediating regulatory networks under salinity Specific classes are discussed detail, glycine-rich (GR-RBPs), serine/arginine-rich splicing factors (SR proteins), zinc finger domain-containing proteins, DEAD-box helicases (DBRHs), KH Pumilio (PUMs), pentatricopeptide repeat (PPRs), involved cytoplasmic granule formation. By integrating their localization current mechanistic insights, this concludes summarizing knowledge highlighting potential future research directions, aiming inspire further investigations into complex network modulating plant facilitating development strategies enhance tolerance.

Language: Английский

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Traveling with purpose: cell-to-cell transport of plant mRNAs

Trends in Cell Biology, Journal Year: 2023, Volume and Issue: 34(1), P. 48 - 57

Published: June 26, 2023

Language: Английский

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Progress in genome-wide identification of RBPs and their role in mitigating stresses, and growth in plants

South African Journal of Botany, Journal Year: 2023, Volume and Issue: 160, P. 132 - 146

Published: July 7, 2023

Language: Английский

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