Origin and Evolution of IQD Scaffolds and Assembled Protein Complexes in Plant Cell Division DOI
Pradeep Dahiya, Samuel Haluška, Jonas Buhl

et al.

Published: Jan. 1, 2023

In ancestors of modern-day streptophyte algae, cell division has undergone a switch from cleavage-like mode to an inside-out mechanism, in which new walls are inserted at the center and expand centrifugally, eventually fusing with maternal wall specific cortical region, termed zone (CDZ) 1-3. This involved stepwise evolution two novel cytoskeleton arrays, phragmoplast preprophase band (PPB). The PPB/phragmoplast system possibly provided basis for tunable orientation, enabled 3D development morphological adaptations required successful colonization terrestrial habitats4. How acquired its functions, however, is still largely enigmatic. Our previous work identified IQ67-DOMAIN8 (IQD8) Arabidopsis thaliana as important determinant PPB formation plane positioning5,6. IQD8 intrinsically disordered scaffold protein that interacts core components CDZ7. Here, through phylogenetic functional analyses, we show IQDs emerged last common ancestor Klebsormidiophyceae Phragmoplastophyta algae. Gradual changes motif composition acquisition likely facilitated diversification terms subcellular localization protein-protein interactions. Cross-complementation studies mutants provide evidence evolutionarily conserved functions land-plant key regulators control. summary, our establishes plant-specific proteins, played role rewiring neofunctionalization interaction networks distinct sites facilitate evolutionary apparatus microtubule general.

Language: Английский

Transposon proliferation drives genome architecture and regulatory evolution in wild and domesticated peppers DOI
Kang Zhang, Hailong Yu, Lingkui Zhang

et al.

Nature Plants, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Language: Английский

Citations

1
T2T reference genome assembly and genome-wide association study reveal the genetic basis of Chinese bayberry fruit quality DOI Creative Commons

Shuwen Zhang,

Zheping Yu,

Li Sun

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(3)

Published: Jan. 30, 2024

Abstract Chinese bayberry (Myrica rubra or Morella rubra; 2n = 16) produces fruit with a distinctive flavor, high nutritional, and economic value. However, previous versions of the genome lack sequence continuity. Moreover, to date, no large-scale germplasm resource association analysis has examined allelic genetic variations determining quality traits. Therefore, in this study, we assembled telomere-to-telomere (T2T) gap-free reference for cultivar ‘Zaojia’ using PacBio HiFi long reads. The resulting 292.60 Mb T2T genome, revealed 8 centromeric regions, 15 telomeres, 28 345 genes. This represents substantial improvement continuity integrity bayberry. Subsequently, re-sequenced 173 accessions, identifying 6 649 674 single nucleotide polymorphisms (SNPs). Further, phenotypic analyses 29 quality-related traits enabled genome-wide study (GWAS), which identified 1937 SNPs 1039 genes significantly associated An SNP cluster pertinent color was on Chr6: 3407532 5 153 151 bp region, harboring two MYB (MrChr6G07650 MrChr6G07660), exhibiting differential expression extreme phenotype transcriptomes, linked anthocyanin synthesis. adjacent, closely gene, MrChr6G07670 (MLP-like protein), harbored an exonic missense variant shown increase production tobacco leaves tenfold. cluster, potentially quantitative trait locus (QTL), collectively regulates color. In conclusion, our presented complete uncovered suite related fruit-quality traits, functional that could be harnessed enhance breeding efficiency bayberries.

Language: Английский

Citations

5
Extracellular RNAs released by plant-associated fungi: from fundamental mechanisms to biotechnological applications DOI Creative Commons
An-Po Cheng, Seomun Kwon,

Trusha Adeshara

et al.

Applied Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 107(19), P. 5935 - 5945

Published: Aug. 12, 2023

Abstract Extracellular RNAs are an emerging research topic in fungal-plant interactions. Fungal plant pathogens and symbionts release small that enter host cells to manipulate physiology immunity. This communication via extracellular between fungi plants is bidirectional. On the one hand, encapsulated inside vesicles as a defense response well for intercellular inter-organismal communication. other recent reports suggest also full-length mRNAs transported within fungal EVs into plants, these might get translated cells. In this review article, we summarize current views fundamental concepts of released by plant-associated fungi, discuss new strategies apply crop protection against pathogens. Key points • plant-fungal Fungi utilize colonization. can be engineered protect

Language: Английский

Citations

11
New Advances in the Study of Regulation of Tomato Flowering-Related Genes Using Biotechnological Approaches DOI Creative Commons
Denis Baranov, Sergey Dolgov, Vadim Timerbaev

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(3), P. 359 - 359

Published: Jan. 25, 2024

The tomato is a convenient object for studying reproductive processes, which has become classic. Such complex processes as flowering and fruit setting require an understanding of the fundamental principles molecular interaction, structures genes proteins, construction signaling pathways transcription regulation, including synchronous actions cis-regulatory elements (promoter enhancer), trans-regulatory (transcription factors regulatory RNAs), transposable epigenetic regulators (DNA methylation acetylation, chromatin structure). Here, we discuss current state research on tomatoes (2017–2023) devoted to function that regulate signal regulation systems using genome-editing technologies, RNA interference gene silencing, overexpression, heterologous expression. Although central candidate these components have been identified, complete picture their relationship yet be formed. Therefore, this review summarizes latest achievements related set. This work attempts display interaction scheme better understand events under consideration.

Language: Английский

Citations

4
Molecular breeding of tomato: Advances and challenges DOI Creative Commons
Minmin Du, Chuanlong Sun, Lei Deng

et al.

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

ABSTRACT The modern cultivated tomato ( Solanum lycopersicum ) was domesticated from pimpinellifolium native to the Andes Mountains of South America through a “two‐step domestication” process. It introduced Europe in 16th century and later widely worldwide. Since late 19th century, breeders, guided by genetics, breeding science, statistical theory, have improved tomatoes into an important fruit vegetable crop that serves both fresh consumption processing needs, satisfying diverse consumer demands. Over past three decades, advancements molecular technologies, represented marker technology, genome sequencing, editing, significantly transformed paradigms. This article reviews research progress field breeding, encompassing sequencing germplasm resources, identification functional genes for agronomic traits, development key technologies. Based on these advancements, we also discuss major challenges perspectives this field.

Language: Английский

Citations

0
Autophagy positively regulates ethylene‐induced colouration in citrus fruits DOI
Ye Guo, Jinli Gong, Ran Hu

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(1)

Published: March 31, 2025

SUMMARY Autophagy is an evolutionarily conserved process in eukaryotes that regulates metabolic reprogramming and organelle recycling response to various environmental signals developmental cues. However, little known about its regulatory mechanism during fruit colouration ripening, which also undergo dramatic cellular alterations. Here, we demonstrate the autophagy pathway activated citrus colouration, colour transition of significantly delayed when blocked. Furthermore, revealed ethylene, a plant hormone crucial for activates through ethylene‐responsive factor, CsERF061. Further analysis CsERF061 directly binds promoter CsATG8h expression, thereby promoting suggesting key determinant ethylene. These findings enhance our understanding offer potential method improve quality future applications.

Language: Английский

Citations

0
VAP27-1 interacts with KCS6 and CER2 to facilitate the biosynthesis of very- long-chain fatty acids DOI
Haiyan Wang, Yifan Li,

Qinyao Wang

et al.

Plant Science, Journal Year: 2025, Volume and Issue: unknown, P. 112489 - 112489

Published: March 1, 2025

Language: Английский

Citations

0
The IQ67‐domain protein IQD1 regulates fruit shape through complex multiprotein interactions in pepper (Capsicum annuum L.) DOI Creative Commons
Lianzhen Mao, Yiyu Shen, Qingzhi Cui

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Summary Natural genetic variation can be used to improve important crop agronomic traits, and understanding the basis of natural in fruit shape help breeders develop pepper varieties that meet market demand. In this study, we identified a QTL controlling length–width ratio by conventional mapping, encoding previously uncharacterized gene CaIQD1 . Reduced expression resulted short wide fruits pepper, whereas heterologous overexpression narrower tomato. Further experiments suggested regulates affecting cell proliferation, expansion morphological changes. also has direct protein interaction with CaOFP20 CaTRM‐like‐CaOFP20. caused become elongated curved, reduced CaTRM‐like led formation rounder fruits. These changes had significant effect on genes related cycle expansion. The CaTRM‐like‐CaOFP20‐CaIQD1 module may thus represent conserved regulatory pathway for shape. showed interactions calmodulin CaCaM7, tubulin CaMAP70‐2 microtubule motor CaKLCR1, suggesting regulation is dynamics mediated Ca 2+ ‐CaM. We found interacts several homologues typically regulate other plant species. summary, our results show acts as core hub regulating through multiple proteins.

Language: Английский

Citations

0
The mutation of CsSUN, an IQD family protein, is responsible for the short and fat fruit (sff) in cucumber (Cucumis sativus L.) DOI

Zhengao Zhang,

Haiqiang Zhang, Jun‐Yan Liu

et al.

Plant Science, Journal Year: 2024, Volume and Issue: 346, P. 112177 - 112177

Published: July 2, 2024

Language: Английский

Citations

2
Comprehensive genetic diversity and genome-wide association studies revealed the genetic basis of avocado fruit quality traits DOI Creative Commons
Jin Li, Shamseldeen Eltaher,

Barbie Freeman

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 13, 2024

Introduction Avocado ( Persea americana ) is a highly nutritious fruit gaining worldwide popularity. However, its cultivation currently reliant on limited number of cultivars with restricted genetic diversity. This study aims to investigate the diversity and population structure avocado germplasm identify loci associated key quality traits that influence customer preference. Methods A panel 110 accessions was analyzed using 4,706 high-quality single nucleotide polymorphisms (SNPs). Genetic were pairwise FST, AMOVA, admixture analysis, phylogenetic analysis. Genome-wide association studies (GWAS) conducted targeting nine two models: General Linear Model (GLM) Principal Component Analysis (PCA) Mixed (MLM) PCA kinship (PCA + K). Results The analysis revealed three distinct populations corresponding ecotypes: Guatemalan, West Indian, Mexican. Phylogenetic indicated closer relationship between Guatemalan Indian races compared Mexican race in our Florida collection. GWAS led identification 12 markers within 11 genomic regions significantly such as color, shape, taste, skin texture. These explained 14.84% 43.96% phenotypic variance, an average 24.63%. Annotation these unveiled candidate genes potentially responsible for controlling traits. Discussion findings enhance understanding germplasm. identified provide valuable insights into basis traits, aiding breeding programs developing improved cultivars. Marker-assisted selection can accelerate development new varieties, promoting more diverse resilient market.

Language: Английский

Citations

2