International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(5), P. 4816 - 4816
Published: March 2, 2023
Wucai
(Brassica
campestris
L.)
is
a
leafy
vegetable
that
originated
in
China,
its
soluble
sugars
accumulate
significantly
to
improve
taste
quality
during
maturation,
and
it
widely
accepted
by
consumers.
In
this
study,
we
investigated
the
sugar
content
at
different
developmental
stages.
Two
periods
including
34
days
after
planting
(DAP)
46
DAP,
which
represent
period
prior
accumulation,
respectively,
were
selected
for
metabolomic
transcriptomic
profiling.
Differentially
accumulated
metabolites
(DAMs)
mainly
enriched
pentose
phosphate
pathway,
galactose
metabolism,
glycolysis/gluconeogenesis,
starch
sucrose
fructose
mannose
metabolism.
By
orthogonal
projection
latent
structures-discriminant
s-plot
(OPLS-DA
S-plot)
MetaboAnalyst
analyses,
D-galactose
β-D-glucose
identified
as
major
components
of
accumulation
wucai.
Combined
with
transcriptome,
pathway
interact
network
between
26
DEGs
two
mapped.
CWINV4,
CEL1,
BGLU16,
BraA03g023380.3C
had
positive
correlations
The
lower
expression
BraA06g003260.3C,
BraA08g002960.3C,
BraA05g019040.3C,
BraA05g027230.3C
promoted
ripening
These
findings
provide
insights
into
mechanisms
underlying
commodity
maturity,
providing
basis
breeding
sugar-rich
wucai
cultivars.
Communications Biology,
Journal Year:
2024,
Volume and Issue:
7(1)
Published: Aug. 30, 2024
Sugar
content
is
a
critical
indicator
of
fruit
quality
and
mainly
controlled
by
sugar
transporters.
Sugars
will
eventually
be
exported
transporters
(SWEET)
proteins
play
an
indispensable
role
in
allocation
between
within
plant
organs.
Sucrose
the
major
many
fruits
predominant
form
translocated
peach
(Prunus
persica).
However,
multiple
SWEET
genes
sucrose
to
remains
elusive.
In
this
study,
total
19
candidates
have
been
identified
genome,
two
Clade
III
genes,
PpSWEET9a
PpSWEET14,
are
found
highly
expressed
mature
source
leaves
branches.
Complementation
assays,
transgene
manipulations,
protein
interaction
studies
reveal
that
PpSWEET14
serve
as
efflux
heterooligomer
synergistically
directs
from
fruits.
Our
findings
provide
insights
into
effect
SWEETs
on
accumulation
identify
genetic
for
improving
quality.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(5), P. 4816 - 4816
Published: March 2, 2023
Wucai
(Brassica
campestris
L.)
is
a
leafy
vegetable
that
originated
in
China,
its
soluble
sugars
accumulate
significantly
to
improve
taste
quality
during
maturation,
and
it
widely
accepted
by
consumers.
In
this
study,
we
investigated
the
sugar
content
at
different
developmental
stages.
Two
periods
including
34
days
after
planting
(DAP)
46
DAP,
which
represent
period
prior
accumulation,
respectively,
were
selected
for
metabolomic
transcriptomic
profiling.
Differentially
accumulated
metabolites
(DAMs)
mainly
enriched
pentose
phosphate
pathway,
galactose
metabolism,
glycolysis/gluconeogenesis,
starch
sucrose
fructose
mannose
metabolism.
By
orthogonal
projection
latent
structures-discriminant
s-plot
(OPLS-DA
S-plot)
MetaboAnalyst
analyses,
D-galactose
β-D-glucose
identified
as
major
components
of
accumulation
wucai.
Combined
with
transcriptome,
pathway
interact
network
between
26
DEGs
two
mapped.
CWINV4,
CEL1,
BGLU16,
BraA03g023380.3C
had
positive
correlations
The
lower
expression
BraA06g003260.3C,
BraA08g002960.3C,
BraA05g019040.3C,
BraA05g027230.3C
promoted
ripening
These
findings
provide
insights
into
mechanisms
underlying
commodity
maturity,
providing
basis
breeding
sugar-rich
wucai
cultivars.
