Quantitative prediction of disinfectant tolerance inListeria monocytogenesusing whole genome sequencing and machine learning DOI Creative Commons
Alexander Gmeiner, Mirena Ivanova, Patrick Murigu Kamau Njage

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 6, 2023

ABSTRACT Listeria monocytogenes is a potentially severe disease-causing bacteria mainly transmitted through food. This pathogen of great concern for public health and the food industry in particular. Many countries have implemented thorough regulations, some even set ‘zero-tolerance’ thresholds particular products to minimise risk L. outbreaks. emphasises that proper sanitation processing plants utmost importance. Consequently recent years, there has been an increased interest tolerance disinfectants used industry. Even though many studies are focusing on laboratory quantification tolerance, possibility predictive models remains poorly studied. Within this study, we explore prediction minimum inhibitory concentrations (MIC) using whole genome sequencing (WGS) machine learning (ML). We WGS data MIC values quaternary ammonium compound (QAC) from 1649 isolates train different ML predictors. Our study shows promising results predicting QAC learning. were able high-performing classifiers predict with balanced accuracy scores up 0.97±0.02. For values, regressors mean squared error as low 0.07±0.02. also identified several new genes related cell wall anchor domains, plasmids, phages, putatively associated disinfectant . The findings first step towards In future, might be monitor production support conceptualisation more nuanced programs. AUTHOR SUMMARY Microbial contamination challenges safety by transmitting harmful microbes such consumers. example bacteria, which primarily can cause diseases at-risk groups. Fortunately, strict regulations stringent cleaning protocols place prevent transmission However, increase industry, reduce their effectiveness. phenotypic accurately whether individual tolerant selected disinfectants. not only distinguish sensitive/tolerant but degrees Further, report important could give information about possible mechanisms. similar guide disinfection facilitate maximum

Language: Английский

Multi-species biofilms of environmental microbiota isolated from fruit packing facilities promoted tolerance of Listeria monocytogenes to benzalkonium chloride DOI Creative Commons
M. Laura Rolon, Olena Voloshchuk, Katie Bartlett

et al.

Biofilm, Journal Year: 2024, Volume and Issue: 7, P. 100177 - 100177

Published: Jan. 21, 2024

may survive and persist in food processing environments due to formation of complex multi-species biofilms environmental microbiota that co-exists these environments. This study aimed determine the effect selected on biofilm tolerance

Language: Английский

Citations

17
Large-scale phenotypic and genomic analysis of Listeria monocytogenes reveals diversity in the sensitivity to quaternary ammonium compounds but not to peracetic acid DOI Creative Commons
Mirena Ivanova, Martin Laage Kragh, Judit Szarvas

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

ABSTRACT Listeria monocytogenes presents a significant concern for the food industry due to its ability persist in processing environment. One of factors contributing persistence is decreased sensitivity disinfectants. Our objective was assess diversity L. disinfectants by testing response 1,671 L . isolates quaternary ammonium compounds (QACs) and 414 peracetic acid (PAA) using broth microdilution growth curve analysis assays, respectively, categorize into sensitive tolerant. A high phenotype-genotype concordance (95%) regarding tolerance QACs obtained screening genomes presence QAC tolerance-associated genes bcrABC , emrE emrC, qacH Based on this concordance, we assessed genes’ dissemination among publicly available ( n = 39,196). Overall, were found 23% 28% collection study global data set, respectively. most prevalent genes, with being detected gene USA, while dominated Europe. No differences P > 0.05) PAA belonging different lineages, serogroups, clonal complexes, or isolation sources, highlighting limited variation disinfectant. The present work represents largest important at phenotypic genomic level, revealing all showed similar PAA. IMPORTANCE Contamination within environments great challenges eradicating once they become established persistent Genetic markers associated increased certain have been identified, which alongside other biotic abiotic can favor production By employing comprehensive large-scale analysis, significantly enhances understanding genetic determinants tolerance. We provide overview prevalence public sequences their distribution geographical locations. Additionally, our shows that disinfectant be used as lack indicates reliable effect no apparent possibility emergence

Language: Английский

Citations

2
Listeria monocytogenes in food businesses: From persistence strategies to intervention/prevention strategies—A review DOI
Tessa Tuytschaever, Katleen Raes, Imca Sampers

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2023, Volume and Issue: 22(5), P. 3910 - 3950

Published: Aug. 7, 2023

Abstract In 2023, Listeria monocytogenes persistence remains a problem in the food business. A profound understanding of how this pathogen persists may lead to better aimed intervention/prevention strategies. The lack uniform definition makes comparison between studies complex. Harborage sites offer protection against adverse environmental conditions and form ideal habitat for formation biofilms, one major retarded growth rate, disinfectant resistance/tolerance, desiccation protozoan complete list strategies can occur on themselves or combination with biofilms. Based discussed strategies, intervention are proposed. By enhancing focus four precaution principles (cleaning disinfection, infrastructure/hygienic design, technical maintenance, work methodology) as mentioned Regulation (EC) No. 852/2004, risk be decreased. All result obtaining maintaining good general hygiene status throughout establishment at all levels ranging from separate equipment entire building.

Language: Английский

Citations

27
Listeria monocytogenes from Food Products and Food Associated Environments: Antimicrobial Resistance, Genetic Clustering and Biofilm Insights DOI Creative Commons
Adriana Silva, Vanessa Silva, João Paulo Gomes

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(5), P. 447 - 447

Published: May 14, 2024

Listeria monocytogenes, a foodborne pathogen, exhibits high adaptability to adverse environmental conditions and is common in the food industry, especially ready-to-eat foods. L. monocytogenes strains pose safety challenges due their ability form biofilms, increased resistance disinfectants, long-term persistence environment. The aim of this study was evaluate presence genetic diversity related products collected from 2014 2022 assess antibiotic susceptibility biofilm formation abilities. identified 13 out 227 (6%) samples, 7 (meat preparation, cheeses, raw milk) 6 food-processing environments (slaughterhouse-floor catering establishments). All isolates exhibited biofilm-forming capacity testing showed several classes antibiotics, trimethoprim-sulfamethoxazole erythromycin. Genotyping core-genome clustering eight sequence types cluster three very closely ST3 (all food), suggesting contamination source. Whole-genome sequencing (WGS) analysis revealed genes conferring fosfomycin (fosX), lincosamides (lin), fluoroquinolones (norB), tetracycline (tetM). In addition, qacJ gene also detected, disinfecting agents antiseptics. Virulence profiling 92 associated with pathogenicity, adherence, persistence. These findings underscore food-associated environments, demonstrating virulence these but disinfectants Moreover, they emphasize need for continuous surveillance, effective risk assessment, rigorous control measures minimize public health risks severe infections, particularly listeriosis outbreaks. A better understanding complex dynamics pathogens can help improve overall develop more strategies prevent consequences economic losses.

Language: Английский

Citations

10
Determination of an Effective Sanitizing Procedure for Listeria innocua in Personal Protective Equipment Used in Dairy Facilities DOI Creative Commons

Karen Nieto-Flores,

Luis Sabillón, Jayne Stratton

et al.

Journal of Food Protection, Journal Year: 2025, Volume and Issue: unknown, P. 100455 - 100455

Published: Jan. 1, 2025

The presence of Listeria monocytogenes in the dairy environment remains a food safety challenge. source microbial contamination may include employees and their personal protective equipment (PPE). This study investigated effectiveness cleaning protocols (i.e., detergents mechanical action) three chemical sanitizers commonly employed at facilities against innocua on different types gloves, aprons, boots. Coupons made PPE material were inoculated with two-strain cocktail L. suspended either Phosphate Buffered Saline (PBS) or skim milk to determine potential effect organic matter. In general, peroxyacetic acid (0.20% (v/v)) was more effective reducing counts aprons gloves compared chlorine quaternary ammonium 200 ppm. Depending type sanitizer, reductions ranged from 1.95 - 4.72 1.52 4.60 log CFU/in2 respectively. comparison, achieved 0.93 2.32 reduction boot soles, no significant differences observed among sanitizers. PVC (vinyl) lower than nitrile latex gloves. Sanitizers less boots wider deeper lugs those shallow lugs. matter significantly reduced antimicrobial efficacy all (<1 reduction). However, inclusion without action ≥3 PPE. highlights importance scrubbing as an essential step reduce

Language: Английский

Citations

1
A Comprehensive Virulence and Resistance Characteristics of Listeria monocytogenes Isolated from Fish and the Fish Industry Environment DOI Open Access
Arkadiusz Zakrzewski, Monika Kurpas, Anna Zadernowska

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3581 - 3581

Published: Feb. 10, 2023

Listeria monocytogenes is an important pathogen, often associated with fish, that can adapt and survive in products food processing plants, where it persist for many years. It a species characterized by diverse genotypic phenotypic characteristics. Therefore, this study, total of 17 L. strains from fish fish-processing environments Poland were their relatedness, virulence profiles, resistance genes. The Core Genome Multilocus Sequence Typing (cgMLST) analysis revealed the most frequent serogroups IIa IIb; sequence types (ST) ST6 ST121; clonal complexes (CC) CC6 CC121. genome multilocus typing was applied to compare present isolates publicly available genomes recovered Europe humans listeriosis. Despite differential subtypes, had similar antimicrobial profiles; however, some genes located on mobile genetic elements could be transferred commensal or pathogenic bacteria. results study showed molecular clones tested characteristic isolated sources. Nevertheless, worth emphasizing they major public health risk due close relation human

Language: Английский

Citations

16
Environmental persistence of Listeria monocytogenes and its implications in dairy processing plants DOI Open Access

Bhaswati Chowdhury,

Sanjeev Anand

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2023, Volume and Issue: 22(6), P. 4573 - 4599

Published: Sept. 7, 2023

Listeriosis, an invasive illness with a fatality rate between 20% and 30%, is caused by the ubiquitous bacterium Listeria monocytogenes. Human listeriosis has long been associated foods. This because nature of bacteria renders it common food contaminant, posing significant risk to processing sector. Although several sophisticated stress coping mechanisms have identified as contributing factors toward pathogen's persistence, complete understanding underlying persistence across various strains remains limited. Moreover, aside from genetic aspects that promote ability cope stress, environmental exist in manufacturing plants could also contribute pathogen. The objective this review provide insight into challenges faced dairy industry pathogens' persistence. Additionally, aims emphasize diverse adaptation response utilized L. monocytogenes evade stressors. environment poses serious threat safety public health. emergence areas high levels contamination facilitate transmission through aerosols, potentially leading recontamination food, particularly floors drains, when sanitation implemented alongside product manufacturing. Hence, produce safe products reduce frequency outbreaks listeriosis, crucial understand pathogen implement efficient control strategies.

Language: Английский

Citations

15
Sensitivity of 240 Listeria monocytogenes isolates to common industrial biocides is more dependent on the presence of residual organic matter or biofilm than on genetic determinants DOI Creative Commons
Martin Laage Kragh, Mirena Ivanova, Paw Dalgaard

et al.

Food Control, Journal Year: 2023, Volume and Issue: 158, P. 110244 - 110244

Published: Dec. 4, 2023

Listeria monocytogenes continues to be a problem in ready-to-eat foods. Tolerance and adaptation biocides have been proposed contribute the persistence of L. food processing industry. This study aimed determine if sensitivity common varies among 240 isolates. The impact residual organic matter (soils), biofilm, ability adapt was also evaluated. Food related isolates (n = 240) were whole genome sequenced assigned sequence types 53) clonal complexes 32). Isolates screened for genetic elements associated with biocide tolerance tested their minimum inhibitory concentration (MIC) benzalkonium chloride (BC), peracetic acid (PAA), sodium hypochlorite (SH) ethanol (ET). Biocide under soiled conditions, biofilm broth suspension test (BST) further 19 representative Adaptation sub-MICs BC, PAA or SH (18 days, 15 °C) MIC (62 mg/l) showed no variation, while (47–94 mg/L) ET (4.7–9.4% v/v) values differed 2 × For an 8 difference (0.3–2.5 observed. Eighty-seven 88 BC tolerant (MIC 2.5 harbored known genes. Soiled conditions increased MIC-values 8–33 but not ET. Biofilm bactericidal concentrations 4–8 SH. Survival genes significantly (p > 0.05) improved BSTs PAA, BC. Lineage I 7 19) < better survival than lineage II 12) when exposed (100 BSTs. (8 increase) occurred exclusively originally sensitive isolates, In conclusion, limited variation assays. However, less efficacy affected by and/or demonstrating need proper cleaning prior use being perturbed.

Language: Английский

Citations

13
High Disinfectant Tolerance in Pseudomonas spp. Biofilm Aids the Survival of Listeria monocytogenes DOI Creative Commons
Gunn Merethe Bjørge Thomassen, Thorben Reiche,

Martinus Hjørungnes

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1414 - 1414

Published: May 27, 2023

Pseudomonas spp. are the most commonly found bacteria in food-processing environments due to properties such as a high growth rate at low temperatures, tolerance of antimicrobial agents, and biofilm formation. In this study, set isolates originating from cleaned disinfected surfaces salmon processing facility were screened for formation 12 °C. A variation between was observed. Selected isolates, both planktonic states, tested resistance/tolerance used disinfectant (peracetic acid-based) antibiotic florfenicol. Most showed much higher state than state. multi-species experiment with five strains without Listeria monocytogenes strain, appeared aid survival L. cells after disinfection, underscoring importance controlling bacterial load environments.

Language: Английский

Citations

12
Exploring the Intricacies of Antimicrobial Resistance: Understanding Mechanisms, Overcoming Challenges, and Pioneering Innovative Solutions DOI

Shahbaz Ul Haq,

Lingchong Wang, Amjad Islam Aqib

et al.

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177511 - 177511

Published: March 1, 2025

Language: Английский

Citations

0