Phylogenomic and phenotypic analyses highlight the diversity of antibiotic resistance and virulence in both human and non-humanAcinetobacter baumannii DOI Open Access
Ellen M. E. Sykes, Valeria Mateo-Estrada,

Raelene Engelberg

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 14, 2023

Abstract Acinetobacter baumannii is a Gram-negative opportunistic pathogen causing infections of the immunocompromised. With high incidence muti-drug resistance, carbapenem-resistant A. as priority 1 designated by WHO. The current literature has expertly characterized clinical isolates baumannii. As challenge these recently been classified One Health issue, we set out to explore diversity from human and non-clinical sources such agricultural surface water, urban streams, various effluents wastewater-treatment plants tank milk; and, importantly, came wide geographic distribution. Phylogenomic analysis considering almost 200 showed that our diverse well-differentiated main international clones . We discovered novel sequence types in both hospital settings, five strains overexpress RND efflux pump adeIJK without changes susceptibility. Further, detected bla ADC-79 non-human isolate despite its sensitivity all antibiotics. There was no significant differentiation between virulence profiles Galleria mellonella insect model suggesting neither dependent on origin nor isolation source. Detection antibiotic resistance genes suggests may act genetic reservoir for strains. This endorses notion order combat multi-drug resistant infection caused baumannii, approach required, deeper understanding must be achieved. Importance global crisis silent one. More more bacteria are becoming antibiotics available treatment, leaving options remaining. includes shows frequency many last-resort drugs carbapenem colistin. Research focused origin, but there knowledge gap regarding traits, particularly, how become notorious today. Antibiotic have animals, environmental locations grass soil. such, concern which health humans, animals environment. Thus, truly crisis, need understand gene reservoirs this under continuum. Repositories NCBI GenBank Accession numbers: Bioproject PRJNA819071, Biosamples SAMN26898552 - SAMN26898587.

Language: Английский

Genomic analysis of diverse environmental Acinetobacter isolates identifies plasmids, antibiotic resistance genes, and capsular polysaccharides shared with clinical strains DOI
Liam A. Tobin, Veronica M. Jarocki, Johanna J. Kenyon

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(2)

Published: Jan. 11, 2024

an important pathogen known for its widespread antibiotic resistance, has been the focus of extensive research within genus, primarily involving clinical isolates. Consequently, data on environmental

Language: Английский

Citations

12
Whole-genome sequencing of Acinetobacter baumannii clinical isolates from a tertiary hospital in Terengganu, Malaysia (2011–2020), revealed the predominance of the Global Clone 2 lineage DOI Creative Commons

Nurul Saidah Din,

Farahiyah Mohd. Rani,

Ahmed Ghazi Alattraqchi

et al.

Microbial Genomics, Journal Year: 2025, Volume and Issue: 11(2)

Published: Feb. 5, 2025

Carbapenem-resistant Acinetobacter baumannii is recognized by the World Health Organization (WHO) as one of top priority pathogens. Despite its public health importance, genomic data clinical isolates from Malaysia remain scarce. In this study, whole-genome sequencing was performed on 126 A . collected main tertiary hospital in state Terengganu, Malaysia, over a 10-year period (2011–2020). Antimicrobial susceptibilities determined for 20 antibiotics belonging to 8 classes showed that 77.0% ( n =97/126) were categorized multidrug resistant (MDR), with all MDR being carbapenem resistant. Multilocus sequence typing analysis Terengganu A. into 34 Pasteur and 44 Oxford types (STs), ST2 Global Clone 2 lineage identified dominant ST =76/126; 60.3%). The could be subdivided six STs majority ST195 =35) ST208 =17). Various antimicrobial resistance genes bla OXA-23 -encoded carbapenemase predominant acquired gene =90/126; 71.4%). Plasmid-encoded rep nearly =122/126; 96.8%) Rep_3 family =121). virulence factors identified, highlighting pathogenic nature bacterium. Only 14/126 (11.1%) positive carriage CRISPR-Cas arrays none prevalent harbouring them. This study provided snapshot obtained single healthcare centre predominance closely related lineage, indicating entrenchment clone hospital.

Language: Английский

Citations

1
Nosocomial surveillance of multidrug-resistant Acinetobacter baumannii : a genomic epidemiological study DOI Creative Commons
Zhimei Duan, Xuming Li, Song Li

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(2)

Published: Jan. 10, 2024

Acinetobacter baumannii is a major opportunistic pathogen causing hospital-acquired infections, and it imperative to comprehend its evolutionary epidemiological dynamics in hospitals prevent control nosocomial transmission. Here, we present comprehensive genomic study involving the sequencing antibiotic resistance profiling of 634 A. strains isolated from seven intensive care units (ICUs) Chinese general hospital over 2 consecutive years. Our reveals that ST2 highly dominant (90.54%) ICUs, with 98.90% exhibiting multidrug resistant or extensively drug resistant. Phylogenetic analyses newly sequenced genomes public data suggest isolates originated outside but evolved inside. The lineages appear be stable, 9 28 identified epidemic clones infecting 60% affected patients. However, outbreaks two have been observed different hospitals, suggesting significant inter-hospital transmission chains. By coupling patient medical records divergence ST2, found cross-ward transfer played crucial role pathogen's Additionally, 831 potential adaptive loci 44 associated genes by grouping comparing prevalence. Overall, our provides contemporary survey on epidemiology evolution large hospital. These findings shed light offers valuable information for prevention therapy.IMPORTANCEThis delved into scale, spanning both an extended time period largest sample size date. Through molecular investigations based genomics, can directly trace origin pathogen, detecting monitoring infectious diseases timely manner, ensuring health safety. In addition, this also collects amount detection data, which helpful phenotype prediction sequencing. It enables patients receive personalized treatment quickly, helps doctors select antibiotics more accurately, contributes reducing use lowering risk development.

Language: Английский

Citations

7
Phylogenomic and phenotypic analyses highlight the diversity of antibiotic resistance and virulence in both human and non-human Acinetobacter baumannii DOI Creative Commons
Ellen M. E. Sykes, Valeria Mateo-Estrada,

Raelene Engelberg

et al.

mSphere, Journal Year: 2024, Volume and Issue: 9(3)

Published: March 5, 2024

is a Gram-negative, opportunistic pathogen that causes infections in the immunocompromised. With high incidence of muti-drug resistance, carbapenem-resistant

Language: Английский

Citations

6
Acinetobacter baumannii clinical isolates resist complement-mediated lysis by inhibiting the complement cascade and improperly depositing MAC. DOI Creative Commons
Michal Magda,

Wendy Boschloo,

Serena Bettoni

et al.

Journal of Innate Immunity, Journal Year: 2025, Volume and Issue: 17(1), P. 112 - 125

Published: Jan. 22, 2025

Introduction: Acinetobacter baumannii is a gram-negative opportunistic bacterium that causes life-threatening infections in immunocompromised hosts. The complement system critical mechanism of innate immunity protects the human body from bacterial infections. Complement activation leads to deposition membrane attack complex (MAC), which can directly lyse bacteria. However, A. has developed evasion mechanisms protect itself complement. Methods: was investigated by flow cytometry and Western blotting. Soluble MAC formation assessed ELISA. Bacterial serum resistance determined SYTOX Green Assay. Galleria mellonella used as an infection model. Genome sequencing revealed virulence genes carried isolates. Results: We examined clinical isolates found 11 with 5 without deposition. Trypsinization MAC-positive significantly reduced MAC, indicating incorrect insertion, consistent lack lysis these strains. MAC-negative inhibited alternative pathway were more serum-resistant. These strains also virulent G. Whole genome genes, both differed capsule type. Importantly, correlation observed between inhibition type (e.g., locus KL171) bacteria, while KL230) associated increased sensitivity MAC-mediated lysis. Conclusion: Our findings suggest relationship type, resistance, host baumannii.

Language: Английский

Citations

0
Genomic plasticity of extensively drug-resistant and multidrug-resistant Acinetobacter baumannii ST208 isolates from a fatal outbreak DOI Creative Commons
Satoshi Nishida, Yasuo Ono

Journal of Infection and Public Health, Journal Year: 2025, Volume and Issue: unknown, P. 102739 - 102739

Published: March 1, 2025

The prevalence of multidrug-resistant Acinetobacter baumannii (MDRA) has rapidly increased and is linked to severe nosocomial infections. MDRA outbreaks in a Japanese hospital were analysed using whole-genome sequencing. Antibiotic susceptibility testing was performed on clinical isolates from hospitalised patients before during the 2009 2010 outbreaks. Whole-genome sequencing conducted identify acquired antibiotic-resistance genes genetic mutations. Clinical A. resistant β-lactams (broad-spectrum cephalosporins carbapenems), aminoglycosides, chloramphenicol, fosfomycin, fluoroquinolones, tetracyclines, trimethoprim-sulfamethoxazole. harboured aac(6')-Ib-cr, abaF, armA, blaADC-30, blaTEM-1, blaOXA-82, or both blaOXA-66 blaOXA-23, catB8, mphE, msrE, tet(B). blaOXA-82 recombinationally multiplied. Quinolone resistance also associated with gyrA S81L parC S84L belonged Oxford sequence type (ST) 208 Pasteur ST2. Three 15 developed an extensively drug-resistant (XDR) phenotype, two adeS mutation. We identified molecular markers three XDR one MDR isolate provided genomic description virulence, as well origins isolates. are closely related ST208 ST2, Asia Australia. concern community settings Western Pacific region.

Language: Английский

Citations

0
Structure of the K141 capsular polysaccharide produced by Acinetobacter baumannii isolate KZ1106 that carries KL141 at the chromosomal K locus DOI Creative Commons
Anastasiya A. Kasimova,

Anna S. Kolganova,

Alexander S. Shashkov

et al.

Carbohydrate Research, Journal Year: 2024, Volume and Issue: 538, P. 109097 - 109097

Published: March 24, 2024

The structure of the K141 type capsular polysaccharide (CPS) produced by Acinetobacter baumannii KZ1106, a clinical isolate recovered from Kazakhstan in 2016, was established sugar analyses and one- two-dimensional

Language: Английский

Citations

3
Whole genome sequencing and genomic characteristics analysis of carbapenem-resistant Acinetobacter baumannii clinical isolates in two hospitals in China DOI Creative Commons

Chongmei Tian,

Lingfang Di,

Su Dong

et al.

Infection Genetics and Evolution, Journal Year: 2024, Volume and Issue: 123, P. 105642 - 105642

Published: July 14, 2024

Nosocomial outbreaks caused by carbapenem-resistant Acinetobacter baumannii (CRAB) strains are rapidly emerging worldwide and cause for concern. Herein, we aimed to describe the genomic characteristics of CRAB isolated from two hospitals in China 2023. The A. isolates were mainly collected ICU sputum (71.43%, 15/21), followed urine (14.29%, 3/21). Twenty-one possessed a multidrug-resistant (MDR) profile, whole-genome sequencing showed that they all carried bla

Language: Английский

Citations

3
Multidrug-Resistant and Extensively Drug-Resistant Acinetobacter baumannii Causing Nosocomial Meningitis in the Neurological Intensive Care Unit DOI Creative Commons
Nadezhda K. Fursova, Mikhail V. Fursov,

Evgeny I. Astashkin

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(8), P. 2020 - 2020

Published: Aug. 6, 2023

Acinetobacter baumannii is one of the significant healthcare-associated meningitis agents characterized by multidrug resistance and a high mortality risk. Thirty-seven A. strains were isolated from thirty-seven patients Moscow neuro-ICU with in 2013–2020. The death rate was 37.8%. Strain susceptibility to antimicrobials determined on Vitek-2 instrument. Whole-genome sequencing conducted using Illumina technology; sequence types (ST), capsular (KL), lipooligosaccharide outer core locus (OCL), antimicrobial genes, virulence genes identified. prevalent ST ST2, belonging international clone IC2, rarer, ST1, ST19, ST45, ST78, ST106, ST400, prevalence KL9 OCL1. Twenty-nine belonged multidrug-resistant (MDR) eight extensively drug-resistant (XDR) categories. Genes conferring beta-lactams (blaPER, blaGES, blaADC, blaCARB, blaCTX-M, blaTEM, blaOXA-types), aminoglycosides (aac, aad, ant, aph, arm), tetracyclines (tet), macrolides (msr mph), phenicols (cml, cat, flo), sulfonamides (dfr sul), rifampin (arr), antiseptics (qac) Virulence nine groups (Adherence, Biofilm formation, Enzymes, Immune evasion, Iron uptake, Regulation, Serum resistance, Stress adaptation, Antiphagocytosis) detected. study highlights heterogeneity genetic clones, variability among meningitis, dominant IC2.

Language: Английский

Citations

9
The Acinetobacter baumannii K70 and K9 capsular polysaccharides consist of related K-units linked by the same Wzy polymerase and cleaved by the same phage depolymerases DOI Creative Commons
Anastasiya A. Kasimova, Nowshin Shyara Sharar, Stephanie J. Ambrose

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(6)

Published: Nov. 17, 2023

Bacteriophage show promise for the treatment of Acinetobacter baumannii infections that resist all therapeutically suitable antibiotics. Many tail-spike depolymerases encoded by phage are able to degrade A. capsular polysaccharide (CPS) exhibit specificity linkage present between K-units make up CPS polymers. This is formed a specific Wzy polymerase, and ability predict this using sequence-based methods identify at K locus could assist with selection therapy. However, little known about polymerase enzymes. Here, we describe can accommodate two different but similar sugars as one residues it links cleave both types bond forms.

Language: Английский

Citations

8