Mechanism of Action of Ribosomally Synthesized and Post-Translationally Modified Peptides

Chemical Reviews, Journal Year: 2022, Volume and Issue: 122(18), P. 14722 - 14814

Published: Sept. 1, 2022

Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a natural product class that has undergone significant expansion due to the rapid growth in genome sequencing data recognition they made by biosynthetic pathways share many characteristic features. Their mode of actions cover wide range biological processes include binding membranes, receptors, enzymes, lipids, RNA, metals as well use cofactors signaling molecules. This review covers currently known modes action (MOA) RiPPs. In turn, mechanisms which these molecules interact with their targets provide rich set molecular paradigms can be used for design or evolution new improved activities given relative ease engineering this review, coverage is limited RiPPs originating from bacteria.

Language: Английский

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A comprehensive review on the utilization of probiotics in aquaculture towards sustainable shrimp farming

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 147, P. 109459 - 109459

Published: Feb. 17, 2024

Language: Английский

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Revisiting the Multifaceted Roles of Bacteriocins

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: Feb. 14, 2024

Abstract Bacteriocins are gene-encoded antimicrobial peptides produced by bacteria. These heterogeneous in terms of structure, activities, biosynthetic clusters, and regulatory mechanisms. widespread nature may contribute to microbial diversity due their capacity target specific Primarily studied as food preservatives therapeutic agents, function natural settings is however less known. This review emphasizes the ecological significance bacteriocins multifunctional exploring bacteriocin distribution, mobility, impact on bacterial population dynamics biofilms.

Language: Английский

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An Insight into Bacteriocins for Combating Microbial Infections and Ensuring Food Safety

International Journal of Peptide Research and Therapeutics, Journal Year: 2025, Volume and Issue: 31(2)

Published: Feb. 6, 2025

Language: Английский

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Escherichia coli has an undiscovered ability to inhibit the growth of both Gram-negative and Gram-positive bacteria

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 28, 2024

Abstract The ability for bacteria to form boundaries between neighboring colonies as the result of intra-species inhibition has been described a limited number species. Here, we report that is more common than previously recognized. We demonstrated swimming four Escherichia coli strains and six other inhibitory zones colonies, which not caused by nutrient depletion. This phenomenon was similarly observed with non-flagellated bacteria. developed square-streaking pattern assay revealed BW25113 inhibits growth E. coli, surprisingly, Gram-positive negative bacteria, including multi-drug resistant clinical isolates. Altogether, our findings demonstrate might be used inhibit Our raise possibility mechanism shared across inhibition. can further explored potential new class antibiotics.

Language: Английский

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Taxonomic distribution of SbmA/BacA and BacA-like antimicrobial peptide transporters suggests independent recruitment and convergent evolution in host–microbe interactions

Microbial Genomics, Journal Year: 2025, Volume and Issue: 11(4)

Published: April 16, 2025

Antimicrobial peptides (AMPs) are often produced by eukaryotes to control bacterial populations in both pathogenic and mutualistic symbioses. Several pathogens nitrogen-fixing legume symbionts depend on transporters called SbmA (or BacA) or BclA (BacA-like) survive exposure AMPs. However, how broadly these distributed amongst bacteria, their evolutionary history, is poorly understood. We used computational approaches, including phylogenetic sequence similarity analyses, examine the distribution of SbmA/BacA proteins across 1,255 species spanning domain Bacteria , leading identification 71 177 proteins, respectively. In vitro sensitivity assays using AMPs several confirmed that AMP transport a common feature homologues. Our analyses indicated homologues encoded only phylum Pseudomonadota primarily found just two orders: Hyphomicrobiales Enterobacterales . somewhat more were clusters four phyla. These included orders phyla Cyanobacteriota order Mycobacteriales (phylum Actinomycetota ) class Negativicutes Bacillota ). Many clades enriched for encoding rich interact with eukaryotic hosts interactions. observations suggest have been repeatedly co-opted facilitate associations allowing bacteria cope host-encoded

Language: Английский

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A Bird’s-Eye View of the Pathophysiologic Role of the Human Urobiota in Health and Disease: Can We Modulate It?

Pathophysiology, Journal Year: 2024, Volume and Issue: 31(1), P. 52 - 67

Published: Feb. 1, 2024

For a long time, urine has been considered sterile in physiological conditions, thanks to the particular structure of urinary tract and production uromodulin or Tamm–Horsfall protein (THP) by it. More recently, development use new technologies, i.e., next-generation sequencing expanded culture, identification microbial community urine, so-called urobiota, became possible. Major phyla detected are represented Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria. Particularly, female urobiota is largely Lactobacillus spp., which very active against pathogenic Escherichia (E.) coli (UPEC) strains via generation lactic acid hydrogen peroxide. Gut dysbiosis accounts for recurrent infections (UTIs), gut–bladder axis syndrome with formation intracellular bacterial communities course acute cystitis. However, other chronic caused intestinal derivation. Monomicrobial polymicrobial account outcome UTIs, even including prostatitis pelvic pain. E. isolates have shown be more invasive resistant antibiotics. Probiotics, fecal transplantation, phage therapy, antimicrobial peptides, immune-mediated therapies, vaccines treatment will described.

Language: Английский

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Turbidimetric bioassays: A solution to antimicrobial activity detection in asymptomatic bacteriuria isolates against uropathogenic Escherichia coli

MicrobiologyOpen, Journal Year: 2024, Volume and Issue: 13(3)

Published: May 6, 2024

Abstract Traditional bacteriocin screening methods often face limitations due to diffusion‐related challenges in agar matrices, which can prevent the peptides from reaching their target organism. Turbidimetric techniques offer a solution these issues, eliminating problems and providing an initial quantification of efficacy producer organisms. This study involved cell‐free supernatant (CFS) eight uncharacterized asymptomatic bacteriuria (ABU) isolates Escherichia coli 83972 for antimicrobial activity against clinical uropathogenic E. (UPEC) strains using turbidimetric growth methods. ABU exhibiting five or more UPEC were further characterized (PUTS 37, PUTS 58, 59, S‐07‐4, SK‐106‐1). The inhibition CFS by proteinase K suggested that was proteinaceous nature, potentially bacteriocins. 58 SK‐106‐1 enhanced artificial urine medium, with both inhibiting all UPECs. A putative microcin H47 operon identified SK‐106‐1, along previously V colicin E7 37 respectively. These findings indicate bacteriocin‐producers could serve as viable prophylactics therapeutics increasing antibiotic resistance among uropathogens.

Language: Английский

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Export of Diverse and Bioactive Small Proteins through a Type I Secretion System

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(5)

Published: April 20, 2023

Small proteins perform a diverse array of functions, from microbial competition, to endocrine signaling, building biomaterials. Microbial systems that can produce recombinant small enable discovery new effectors, exploration sequence activity relationships, and have the potential for in vivo delivery. However, we lack simple controlling small-protein secretion Gram-negative bacteria. Microcins are antibiotics secreted by bacteria inhibit growth neighboring microbes. They exported cytosol environment one-step process through specific class type I (T1SSs). relatively little is known about substrate requirements microcin T1SSs. Here, investigate prototypic V T1SS Escherichia coli show it export remarkably wide range natural synthetic proteins. We demonstrate largely independent cargo protein's chemical properties appears be constrained only protein length. varied bioactive sequences, including an antibacterial protein, signaling factor, protease inhibitor, human hormone, all elicit their intended biological effect. Secretion this system not limited E. coli, its function additional species inhabit gastrointestinal tract. Our findings uncover highly promiscuous nature T1SS, which has implications native-cargo capacity use research IMPORTANCE Type transport cytoplasm extracellular single step. In nature, each generally paired with protein. know these transporters how influences secretion. system. Remarkably, our studies composition Furthermore, used colonize These expand understanding uses variety applications.

Language: Английский

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Human microbiota peptides: important roles in human health

Natural Product Reports, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

This review highlights the functions of bioactive peptides derived from human microbiota in various health issues including antimicrobial defense and resistance, thereby facilitating development personalised healthcare strategies.

Language: Английский

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