The connectome of an insect brain

Science, Journal Year: 2023, Volume and Issue: 379(6636)

Published: March 10, 2023

Brains contain networks of interconnected neurons and so knowing the network architecture is essential for understanding brain function. We therefore mapped synaptic-resolution connectome an entire insect ( Drosophila larva) with rich behavior, including learning, value computation, action selection, comprising 3016 548,000 synapses. characterized neuron types, hubs, feedforward feedback pathways, as well cross-hemisphere brain-nerve cord interactions. found pervasive multisensory interhemispheric integration, highly recurrent architecture, abundant from descending neurons, multiple novel circuit motifs. The brain’s most circuits comprised input output learning center. Some structural features, multilayer shortcuts nested loops, resembled state-of-the-art deep architectures. identified provides a basis future experimental theoretical studies neural circuits.

Language: Английский

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Neuronal wiring diagram of an adult brain

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 124 - 138

Published: Oct. 2, 2024

Language: Английский

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Neuronal wiring diagram of an adult brain

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 30, 2023

Abstract Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains reconstruct local connectivity maps that are highly informative, yet inadequate for understanding function more globally. Here, we present the first neuronal wiring diagram a whole adult brain, containing 5×10 7 chemical synapses ∼130,000 reconstructed from female Drosophila melanogaster . The resource also incorporates annotations cell classes types, nerves, hemilineages, predictions neurotransmitter identities. Data products available download, programmatic access, interactive browsing made interoperable with other fly data resources. We show how derive projectome, map projections regions, connectome. demonstrate tracing synaptic pathways analysis information flow inputs (sensory ascending neurons) outputs (motor, endocrine, descending neurons), across both hemispheres, central optic lobes. Tracing subset photoreceptors all way motor illustrates structure uncover putative circuit mechanisms underlying sensorimotor behaviors. technologies open ecosystem FlyWire Consortium set stage future large-scale connectome projects in species.

Language: Английский

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Volume electron microscopy

Nature Reviews Methods Primers, Journal Year: 2022, Volume and Issue: 2(1)

Published: July 7, 2022

Language: Английский

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Neural signal propagation atlas of Caenorhabditis elegans

Nature, Journal Year: 2023, Volume and Issue: 623(7986), P. 406 - 414

Published: Nov. 1, 2023

Abstract Establishing how neural function emerges from network properties is a fundamental problem in neuroscience 1 . Here, to better understand the relationship between structure and of nervous system, we systematically measure signal propagation 23,433 pairs neurons across head nematode Caenorhabditis elegans by direct optogenetic activation simultaneous whole-brain calcium imaging. We sign (excitatory or inhibitory), strength, temporal causal direction these create functional atlas. find that differs model predictions are based on anatomy. Using mutants, show extrasynaptic signalling not visible anatomy contributes this difference. identify many instances dense-core-vesicle-dependent signalling, including timescales less than second, evoke acute transients—often where no wired connection exists but relevant neuropeptides receptors expressed. propose that, such cases, extrasynaptically released serve similar classical neurotransmitters. Finally, our measured atlas predicts dynamics spontaneous activity do models conclude both synaptic drive short timescales, measurements evoked crucial for interpreting function.

Language: Английский

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Automated synapse-level reconstruction of neural circuits in the larval zebrafish brain

Nature Methods, Journal Year: 2022, Volume and Issue: 19(11), P. 1357 - 1366

Published: Oct. 24, 2022

Abstract Dense reconstruction of synaptic connectivity requires high-resolution electron microscopy images entire brains and tools to efficiently trace neuronal wires across the volume. To generate such a resource, we sectioned imaged larval zebrafish brain by serial block-face at voxel size 14 × 25 nm 3 . We segmented resulting dataset with flood-filling network algorithm, automated detection chemical synapses validated results comparisons transmission microscopic light-microscopic reconstructions. Neurons their connections are stored in form queryable expandable digital address book. reconstructed 208 neurons involved visual motion processing, most them located pretectum, which had been functionally characterized same specimen two-photon calcium imaging. Moreover, mapped all 407 presynaptic postsynaptic partners two superficial interneurons tectum. The resource developed here serves as foundation for synaptic-resolution circuit analyses nervous system.

Language: Английский

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Neuroscience Needs Network Science

Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 43(34), P. 5989 - 5995

Published: Aug. 23, 2023

The brain is a complex system comprising myriad of interacting elements, posing significant challenges in understanding its structure, function, and dynamics. Network science has emerged as powerful tool for studying such intricate systems, offering framework integrating multiscale data complexity. Here, we discuss the application network study brain, addressing topics models metrics, connectome, role dynamics neural networks. We explore opportunities multiple streams transitions from development to healthy function disease, potential collaboration between neuroscience communities. underscore importance fostering interdisciplinary through funding initiatives, workshops, conferences, well supporting students postdoctoral fellows with interests both disciplines. By uniting communities, can develop novel network-based methods tailored circuits, paving way towards deeper functions.

Language: Английский

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Whole-body integration of gene expression and single-cell morphology

Cell, Journal Year: 2021, Volume and Issue: 184(18), P. 4819 - 4837.e22

Published: Aug. 10, 2021

Animal bodies are composed of cell types with unique expression programs that implement their distinct locations, shapes, structures, and functions. Based on these properties, assemble into specific tissues organs. To systematically explore the link between cell-type-specific gene morphology, we registered an atlas to a whole-body electron microscopy volume nereid Platynereis dumerilii. Automated segmentation cells nuclei identifies major classes establishes activation, chromatin topography, nuclear size. Clustering segmented according reveals spatially coherent tissues. In brain, genetically defined groups neurons match ganglionic projections. Besides interneurons, uncover sensory-neurosecretory in mushroom bodies, which thus qualify as sensory They furthermore resemble vertebrate telencephalon by molecular anatomy. We provide integrated browser Fiji plugin for remote exploration all available multimodal datasets.

Language: Английский

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The Nereid on the rise: Platynereis as a model system

EvoDevo, Journal Year: 2021, Volume and Issue: 12(1)

Published: Sept. 27, 2021

The Nereid Platynereis dumerilii (Audouin and Milne Edwards (Annales des Sciences Naturelles 1:195-269, 1833) is a marine annelid that belongs to the Nereididae, family of errant polychaete worms. shows pelago-benthic life cycle: as general characteristic for superphylum Lophotrochozoa/Spiralia, it has spirally cleaving embryos developing into swimming trochophore larvae. larvae then metamorphose benthic worms living in self-spun tubes on macroalgae. used model genetics, regeneration, reproduction biology, development, evolution, chronobiology, neurobiology, ecology, ecotoxicology, most recently also connectomics single-cell genomics. Research started with studies eye development spiralian embryogenesis nineteenth early twentieth centuries. Transitioning molecular era, research focused posterior growth neuroendocrinology, circadian lunar cycles, fertilization, oocyte maturation. Other work covered segmentation, photoreceptors other sensory cells, nephridia, population dynamics. Most recently, unique advantages young worm whole-body volume electron microscopy sequencing became apparent, enabling tracing all neurons its rope-ladder-like central nervous system, construction multimodal cellular atlases. Here, we provide an overview current topics methodologies P. dumerilii, aim stimulating further interest our expanding active vibrant community.

Language: Английский

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Connectomics of the Octopus vulgaris vertical lobe provides insight into conserved and novel principles of a memory acquisition network

eLife, Journal Year: 2023, Volume and Issue: 12

Published: July 6, 2023

Here, we present the first analysis of connectome a small volume

Language: Английский

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