Microbial risks associated with microplastics in the food chain and possible control measures (literature review). Part 1. Dietary intake and influence on the gut microbiota DOI Open Access
Sheveleva Sa, Yu. M. Markova, N. R. Efimochkina

et al.

Hygiene and Sanitation, Journal Year: 2023, Volume and Issue: 102(12), P. 1334 - 1347

Published: Dec. 28, 2023

Environmental contamination by polymer wastes and microplastics (MPs) has recently become important for health care, due to the emergence of a lot evidence that MPs affect living organisms, including humans. commonly presented in drinking water various groups food products, are found human stool, colon tissue, blood samples. When orally ingested, first object their interaction with organism is gastrointestinal microbiota. Considering essential importance intestinal microbiota health, study negative consequences such contact becomes very important. The results “in vitro” vivo” experimental studies summarized review indicate have effects on microbial community composition barrier state, themselves subject degradation tract. “In studies, entry into intestine accompanied an increase α-diversity microbiota, presumably foreign microorganisms attached particles, those as part biofilms formed surfaces. Competing representatives normal flora, these able enzymatically degrade or overcome mucosal barrier. Simultaneously biofilm matrix associate mucus provides particles retention mucin layer direct apical epitheliocytes. This leads irritation, local inflammation, damage biotransformation products can also systemically host organism, translocating from bloodstream. emphasizes identify characterize risks associated intake food, assessing pathways necessary.

Language: Английский

High-throughput anaerobic screening for identifying compounds acting against gut bacteria in monocultures or communities DOI
Patrick Müller, Jacobo de la Cuesta‐Zuluaga, Michael Kuhn

et al.

Nature Protocols, Journal Year: 2023, Volume and Issue: 19(3), P. 668 - 699

Published: Dec. 13, 2023

Language: Английский

Citations

17
Response, resistance, and recovery of gut bacteria to human-targeted drug exposure DOI Creative Commons
Jacobo de la Cuesta‐Zuluaga, Leonardo Boldt, Lisa Maier

et al.

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(6), P. 786 - 793

Published: June 1, 2024

Survival strategies of human-associated microbes to drug exposure have been mainly studied in the context bona fide pathogens exposed antibiotics. Less well understood are survival non-pathogenic and host-associated commensal communities variety drugs xenobiotics which humans exposed. The lifestyle microbial commensals within complex offers a ways adapt different drug-induced stresses. Here, we review responses employed by gut when drugs-antibiotics non-antibiotics-at individual community level. We also discuss factors influencing recovery establishment new structure following exposure. These key stability resilience microbiome, ultimately overall health well-being host.

Language: Английский

Citations

7
Nutrient competition predicts gut microbiome restructuring under drug perturbations DOI Open Access
Handuo Shi, Daniel Newton, Taylor H. Nguyen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 6, 2024

Human gut commensal bacteria are routinely exposed to various stresses, including therapeutic drugs, and collateral effects difficult predict. To systematically interrogate community-level of drug perturbations, we screened stool-derived

Language: Английский

Citations

2
Emergent survival and extinction of species within gut bacterial communities DOI Creative Commons
Naomi Iris van den Berg, Melanie Tramontano, Rui Guan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 29, 2024

Synthetic communities can help uncover metabolic forces shaping microbial ecosystems. Yet, in case of the gut microbiota, culturing undefined media has prevented detection dependencies. Here we show, using chemically defined media, how species survival is jointly determined by supplied resources and community metabolism. We used 63 representative bacterial strains varied inoculum compositions to assemble stable 14 media. Over 95% showed markedly improved or diminished performance relative monoculture at least one condition, including 153 cases (21%) emergent survival, i.e., incapable surviving on their own but thriving a community, 252 (35%) community-driven extinctions. Through single additions exclusions, metabolomic analysis, ecological modelling, demonstrate inter-species dependencies – especially poor are mediated biotic nutrient supply. Our results highlight communal dividend as key force promoting diversity.

Language: Английский

Citations

1
Long-term consequences of drug usage on the gut microbiome DOI
Oliver Aasmets, Nele Taba, Kertu Liis Krigul

et al.

Published: July 17, 2024

Abstract Medication usage is a significant contributor to the inter-individual variability in gut microbiome. However, drugs are often used long-term and repeatedly, notion yet unaccounted for microbiome studies, which might lead underestimating extent of drug effects. Recently, we others showed that not only antibiotics antidepressants at time sampling but also past consumption associated with This effect can be “additive” - more used, stronger on Here, by utilizing electronic health records Estonian Microbiome cohort metagenomics dataset (n=2,509), systematically evaluate effects human-targeted medications We show microbiome, example, antibiotics, psycholeptics, antidepressants, proton pump inhibitors, beta-blockers detectable several years after usage. Furthermore, analyzing subcohort (n=328) measured similar changes occur treatment initiation, possibly indicating causal

Language: Английский

Citations

1
Off-purpose activity of industrial and agricultural chemicals against human gut bacteria DOI Creative Commons
Anna E. Lindell, Stephan Kamrad, Indra Roux

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Abstract Contamination by industrial and agricultural chemicals like pesticides are a cause of great concern due to the risk human environmental health. While these often considered have restricted activity labelled as such, there concerns over broader toxicity range. Here we report impact 1076 pollutants spanning diverse chemistries indicated applications on 22 prevalent commensal gut bacteria. Our systematic investigation uncovered 588 interactions involving 168 chemicals, majority which were not previously reported antibacterial properties. Fungicides showed largest with circa 30% exhibiting anti-commensal We find that sensitivity chemical across species surprisingly correlates human-targeted drugs, suggesting common susceptibility mechanisms. Using genome-wide chemical-genetic screen, identified membrane transport fatty acid metabolism major modulators off-target chemicals. Mutants resistance include those defective in producing human-health-relevant metabolites branched short-chain acids, indicating chronic exposure could lead selection against production beneficial metabolites. Toxicokinetic modelling suggested bacteria be used more sensitive vitro indicators for than animal models. Together, our data uncovers widespread Impact structure function microbiota should therefore assessing safety.

Language: Английский

Citations

1
Non-antibiotic drugs break colonization resistance against pathogenicGammaproteobacteria DOI Creative Commons
Anne Grießhammer, Jacobo de la Cuesta‐Zuluaga, Taiyeb Zahir

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 6, 2023

Summary Non-antibiotic drugs can alter the composition of gut microbiome, with largely undefined implications for human health. Here we compared susceptibility commensal and pathogenic bacteria to non-antibiotic found that pathogens show higher drug resistance, which could favor their expansion after treatment. We then developed a model system screen drug-microbiome interactions increase risk enteropathogenic infections. Approximately 35% >50 tested increased abundance Salmonella Typhimurium in synthetic stool-derived microbial communities. This was due direct effects non-antibiotics on individual commensals, altered within communities potential exploit different metabolic niches. favored vitro also promoted other enteric loads gnotobiotic conventional mice. These findings may inform future strategies control pathogen proliferation assess microbiota-drug-pathogen risks infection.

Language: Английский

Citations

2
Proton-pump inhibitors increaseC. difficileinfection risk by altering pH rather than by affecting the gut microbiome based on a bioreactor model DOI

Julia Schumacher,

Patrick Müller, Johannes Sulzer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 10, 2024

Abstract Clostridioides difficile infections often occur after antibiotic use, but they have also been linked to proton-pump inhibitor (PPI) therapy. The underlying mechanism—whether infection risk is due a direct effect of PPIs on the gut microbiome or changes in gastrointestinal pH—has remained unclear. To disentangle both possibilities, we studied impact omeprazole and pH key members human stool-derived microbial communities from different donors vitro . We then developed custom multiple-bioreactor system grow model community chemostat mode tested effects exposure, changes, their combination C. growth within this community. Our findings show that significantly affect community’s biomass abundances strains, leading increased However, treatment alone did not result such effects. These imply higher following therapy probably because alterations rather than interaction between drug microbiome. This understanding paves way for reducing risks

Language: Английский

Citations

0
Emergent metabolic interactions in resistance toClostridioides difficileinvasion DOI Creative Commons
Achuthan Ambat, Naomi Iris van den Berg, Francisco Zorrilla

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Commensal gut bacteria are key contributors to the resilience against pathogen invasion. This is exemplified by success of fecal microbiota transplantation in treating recurrent Clostridioides difficile infection. Yet, characteristics communities that can confer colonization resistance and underlying mechanisms remain largely unknown. Here we use a synthetic community 14 commensal uncover inter-species interactions metabolic pathways underpinning emergent C. We challenged this as well fecal-matter-derived with antibiotic treatment continuous flow bioreactor. Using generalized Lotka-Volterra genome-scale modelling, identified between Escherichia coli Bacteroides/Phocaeicola sp. pathogen's suppression. Metabolomics analysis further revealed fructooligosaccharide metabolism, vitamin B3 biosynthesis, competition for Stickland metabolism precursors contribute Analysis metagenomics data from patient cohorts clinical trials attested vivo relevance ratio Bacteroides successful resistance. The latter was found be much stronger discriminator than commonly used alpha diversity metrics. Our study uncovers microbial implications rational design bacteriotherapies.

Language: Английский

Citations

0
Microbial risks associated with microplastics in the food chain and possible control measures (literature review). Part 1. Dietary intake and influence on the gut microbiota DOI Open Access
Sheveleva Sa, Yu. M. Markova, N. R. Efimochkina

et al.

Hygiene and Sanitation, Journal Year: 2023, Volume and Issue: 102(12), P. 1334 - 1347

Published: Dec. 28, 2023

Environmental contamination by polymer wastes and microplastics (MPs) has recently become important for health care, due to the emergence of a lot evidence that MPs affect living organisms, including humans. commonly presented in drinking water various groups food products, are found human stool, colon tissue, blood samples. When orally ingested, first object their interaction with organism is gastrointestinal microbiota. Considering essential importance intestinal microbiota health, study negative consequences such contact becomes very important. The results “in vitro” vivo” experimental studies summarized review indicate have effects on microbial community composition barrier state, themselves subject degradation tract. “In studies, entry into intestine accompanied an increase α-diversity microbiota, presumably foreign microorganisms attached particles, those as part biofilms formed surfaces. Competing representatives normal flora, these able enzymatically degrade or overcome mucosal barrier. Simultaneously biofilm matrix associate mucus provides particles retention mucin layer direct apical epitheliocytes. This leads irritation, local inflammation, damage biotransformation products can also systemically host organism, translocating from bloodstream. emphasizes identify characterize risks associated intake food, assessing pathways necessary.

Language: Английский

Citations

1