Reviewer #3 (Public Review): A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access

Published: July 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Language: Английский

Neuronal wiring diagram of an adult brain DOI Creative Commons
Sven Dorkenwald, Arie Matsliah, Amy Sterling

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 124 - 138

Published: Oct. 2, 2024

Language: Английский

Citations

134
Connectome-driven neural inventory of a complete visual system DOI Creative Commons
Aljoscha Nern, Frank Loesche, Shin-ya Takemura

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Abstract Vision provides animals with detailed information about their surroundings and conveys diverse features such as colour, form movement across the visual scene. Computing these parallel spatial requires a large network of neurons. Consequently, from flies to humans, regions in brain constitute half its volume. These often have marked structure–function relationships, neurons organized along maps shapes that directly relate roles processing. More than century anatomical studies catalogued detail cell types fly systems 1–3 , behavioural physiological experiments examined capabilities flies. To unravel diversity complex system, careful mapping neural architecture matched tools for targeted exploration this circuitry is essential. Here we present connectome right optic lobe male Drosophila melanogaster acquired using focused ion beam milling scanning electron microscopy. We established comprehensive inventory developed computational framework quantify anatomy. Together, data establish basis interpreting how vision. By integrating analysis connectivity information, neurotransmitter identity expert curation, classified approximately 53,000 into 732 types. are systematically described newly named. Finally, share an extensive collection split-GAL4 lines our neuron-type catalogue. Overall, set unlocks new possibilities systematic investigations vision foundation deeper understanding sensory

Language: Английский

Citations

2
A split-GAL4 driver line resource forDrosophilaneuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 10, 2024

Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

12
eLife assessment: A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access
Tânia Reis

Published: July 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Language: Английский

Citations

4
A split-GAL4 driver line resource for Drosophila neuron types DOI Open Access
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

Published: Jan. 6, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

0
A split-GAL4 driver line resource for Drosophila neuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 24, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

0
A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

Published: July 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Language: Английский

Citations

3
Author response: A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

Published: July 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Language: Английский

Citations

1
Temperature cues are integrated in a flexible circadian neuropeptidergic feedback circuit to remodel sleep-wake patterns in flies DOI Creative Commons

Xin Yuan,

Hailiang Li, Fang Guo

et al.

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(12), P. e3002918 - e3002918

Published: Dec. 2, 2024

Organisms detect temperature signals through peripheral neurons, which relay them to central circadian networks drive adaptive behaviors. Despite recent advances in Drosophila research, how circuits integrate cues with regulate sleep/wake patterns remains unclear. In this study, we used the FlyWire brain electron microscopy connectome map neuronal connections, identifying lateral posterior neurons LPNs as key nodes for integrating information into network. receive input from both and temperature-sensing promoting sleep behavior. Through analysis, genetic manipulation, behavioral assays, demonstrated that LPNs, downstream of thermo-sensitive anterior cells (ACs), suppress activity-promoting dorsal LNds via AstC pathway, inducing Disrupting LPN-LNd communication either AstCR1 RNAi or an mutant significantly impairs heat-induced reduction evening activity peak. Conversely, optogenetic calcium imaging assays revealed cold-activated subsequently stimulate NPF-NPFR signaling, establishing a negative feedback loop. This mechanism limits LNd activation appropriate levels, thereby fine-tuning peak increase at lower temperatures. conclusion, our study constructed comprehensive centered on identified novel peptidergic circuit coordinates signals, offering new insights regulation .

Language: Английский

Citations

1
A split-GAL4 driver line resource for Drosophila CNS cell types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: July 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

0