Can biocontrol be the game-changer in integrated pest management? A review of definitions, methods and strategies

Journal of Plant Diseases and Protection, Journal Year: 2024, Volume and Issue: 131(2), P. 265 - 291

Published: Feb. 23, 2024

Abstract Global agriculture is heavily dependent on sustainable plant protection. Worldwide, the concept of integrated pest management (IPM) being followed. IPM utilizes a range strategies, with chemical synthetic pesticides employed only as last resort. However, in agricultural practice, farmers continue to rely primarily this option. To further reduce dependence, new strategies are sought strengthen use biological control within approach including identification novel non-synthetic natural compounds. Here, we discuss and report state art research areas such biocontrol agents application ecological principles. These practices can help establish protection systems, greatest impact achieved when they used appropriate combinations. We highlight conditions that currently prevent or hinder increased measures. On background agroecological experiences, why additional advancements imperative more effectively break life cycles pests, diseases weeds. emphasize significance judicious technologies, adapted local conditions. Additionally, key role expertise operators implementing these their knowledge thereof.

Language: Английский

---

Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 25, 2025

In vertebrates and plants, dsRNA plays crucial roles as PAMP a mediator of RNAi. How higher fungi respond to is not known. We demonstrate that Magnaporthe oryzae (Mo), globally significant crop pathogen, internalizes across broad size range 21 about 3000 bp. Incubation fungal conidia with 10 ng/µL dsRNA, regardless or sequence, induced aberrant germ tube elongation, revealing strong sequence-unspecific effect in this fungus. Accordingly, the synthetic analogue poly(I:C) various sizes sequences elicited canonical stress pathways, including nuclear accumulation marker mitogen-activated protein kinase Hog1p production ROS. Leaf application cereal model species Brachypodium distachyon suppressed progression leaf blast disease. Notably, depends on doses, while pure sequence-specific effects were observed at low concentrations ( < 0.03 ng/µL). The protective further enhanced by maintaining gap least seven days between inoculation, stabilising alginate-chitosan nanoparticles. Overall, our study opens up additional possibilities for development use pesticides agriculture.

Language: Английский

---

Exogenous dsRNA-Mediated Control of Rice Blast Fungus Involves Sequence-Specific RNAi and Sequence-Unspecific Fungal Stress Responses

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 30, 2024

In vertebrates and plants, double-stranded (ds)RNA plays crucial roles as a pathogen-associated molecular pattern (PAMP) mediator of RNA interference (RNAi). However, our understanding fungal responses to dsRNA remains limited. Here, we demonstrate that Magnaporthe oryzae (Mo), globally significant crop pathogen, actively internalizes exogenous across broad size range 21 about 3000 bp. Treatment Mo conidia with dsRNA, irrespective or sequence, induces aberrant germ tube elongation. Additionally, application Brachypodium distachyon leaves suppresses disease progression upon infection. Intriguingly, elicits canonical stress pathways in Mo, evidenced by nuclear accumulation the marker mitogen-activated protein kinase Hog1p production reactive oxygen species. these sequence-nonspecific effects are transient, while sequence-specific RNAi activity predominates preventive intervention scenario, when time interval several days between inoculation is maintained, stabilized novel alginate-chitosan nanoparticles. Overall, data show on fungi diseases they cause can be multifaceted complex, which has implications for development pesticides agricultural practice.

Language: Английский

---

The antisense CircRNAVvcircABH controls salt tolerance and the brassinosteroid signaling response by suppressing cognate mRNA splicing in grape

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Summary Soil salinization is a major factor limiting the sustainable development of grape industry. Circular RNAs (circRNAs) are more stable than linear mRNAs and involved in stress responses. However, biological functions molecular mechanisms underlying antisense circRNAs plants remain unclear. We identified circRNA VvcircABH through high‐throughput sequencing. Using genetic transformation methods techniques, we analyzed effects on response to salt its effects. was located nucleus upregulated by stress, while expression level cognate gene VvABH (alpha/beta‐hydrolase) downregulated. overexpression or silencing greatly enhanced tolerance. could bind overlapping region inhibits pre‐mRNA splicing, thereby decreasing . Additionally, repressed additive effect interaction between VvBRI1 (brassinosteroid‐insensitive 1) VvBKI1 (BRI1 kinase inhibitor 1), thus influencing plant's BR, which plays important roles plant conclude that play distinct tolerance brassinosteroid signaling response, govern inhibiting splicing.

Language: Английский

---