Contribution of membrane-associated oscillators to biological timing at different timescales

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 9, 2024

Environmental rhythms such as the daily light-dark cycle selected for endogenous clocks. These clocks predict regular environmental changes and provide basis well-timed adaptive homeostasis in physiology behavior of organisms. Endogenous are oscillators that based on positive feedforward negative feedback loops. They generate stable even under constant conditions. Since weak interactions between allow autonomous synchronization, coupling/synchronization provides self-organized physiological timing. Amongst most thoroughly researched circadian clock neurons mammals insects. comprise nuclear clockworks transcriptional/translational loops (TTFL) ∼24 h gene expression entrained to day-night cycle. It is generally assumed this TTFL clockwork drives all oscillations within cells, being any rhythm Instead current gene-based hierarchical model we here a systems view We suggest coupled system posttranslational loop (PTFL) oscillators/clocks run at multiple timescales governs adaptive, dynamic behavior. focus mammalian insect timescales. neuronal plasma membrane-associated signalosomes constitute specific PTFL localized but interlinked membrane potential intracellular messengers with frequencies. In each neuron multiscale form temporally structured oscillatory network common complex frequency-band comprising superimposed oscillations. Coupling oscillator/clock next level complexity an network. This systemic molecular cellular suggested cycles through homeostatic setpoints characteristic hallmark. propose mechanisms plasticity maintain stability these setpoints, whereas Hebbian enables switching via coupling factors, like biogenic amines and/or neuropeptides. reprogram new frequency, setpoint. Our novel hypothesis up experimental challenge.

Language: Английский

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Circadian Rhythm Regulation by Pacemaker Neuron Chloride Oscillation in Flies

Physiology, Journal Year: 2024, Volume and Issue: 39(3), P. 157 - 166

Published: Feb. 27, 2024

Circadian rhythms in physiology and behavior sync organisms to external environmental cycles. Here, circadian oscillation intracellular chloride central pacemaker neurons of the fly, Drosophila melanogaster, is reviewed. Intracellular links SLC12 cation-coupled transporter function with kinase signaling regulation inwardly rectifying potassium channels.

Language: Английский

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Chloride oscillation in pacemaker neurons regulates circadian rhythms through a chloride-sensing WNK kinase signaling cascade

Current Biology, Journal Year: 2022, Volume and Issue: 32(6), P. 1429 - 1438.e6

Published: March 1, 2022

Language: Английский

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High-Frequency Neuronal Bursting is Essential for Circadian and Sleep Behaviors inDrosophila

Journal of Neuroscience, Journal Year: 2020, Volume and Issue: 41(4), P. 689 - 710

Published: Dec. 1, 2020

Circadian rhythms have been extensively studied in Drosophila ; however, still little is known about how the electrical properties of clock neurons are specified. We performed a behavioral genetic screen through downregulation candidate ion channels lateral ventral (LNvs) and show that hyperpolarization-activated cation current I h important for behaviors LNvs influence: temporal organization locomotor activity, analyzed males, sleep, females. Using whole-cell patch clamp electrophysiology we demonstrate small (sLNvs) bursting neurons, necessary to achieve high-frequency firing pattern characteristic both types Since bursts has associated neuropeptide release, hypothesized would be communication. Indeed, herein fundamental recruitment pigment dispersing factor (PDF) filled dense core vesicles (DCVs) terminals at dorsal protocerebrum their timed hence coordination circadian behaviors. SIGNIFICANCE STATEMENT Ion transmembrane proteins with selective permeability specific charged particles. The rich repertoire parameters may gate opening state, such as voltage-sensitivity, modulation by second messengers kinetics, make this protein family determinant neuronal identity. channel structure evolutionary conserved between vertebrates invertebrates, making any discovery easily translatable. Through uncover roles rhythms, identified an player subset fruit fly. need fire action potentials mode peptide transport control behavior.

Language: Английский

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A Robust and Self-Sustained Peripheral Circadian Oscillator Reveals Differences in Temperature Compensation Properties with Central Brain Clocks

iScience, Journal Year: 2020, Volume and Issue: 23(8), P. 101388 - 101388

Published: July 20, 2020

Circadian clocks are characterized by three properties: they run in constant conditions with a period of ∼24 h, synchronize to the environmental cycles light and temperature, temperature compensated, meaning do not speed up temperature. Central brain regulate daily activity rhythms, whereas peripheral dispersed throughout body insects vertebrates. Using set luciferase reporter genes, we show that Drosophila self-sustained but over-compensated, i.e., slow down increasing In contrast, central clock neurons fly brain, both intact flies cultured brains, accurate compensation. Although this suggests neural network properties contribute compensation, circadian neuropeptide Pigment Dispersing Factor (PDF) is required for temperature-compensated oscillations neurons. Our findings reveal fundamental difference between clocks, which likely also applies vertebrate clocks.

Language: Английский

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